BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0019.p (408 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depo... 731 0.0 gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] d... 364 e-102 gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthet... 84 3e-17 >gi|300309974|ref|YP_003774066.1| poly-beta-hydroxyalkanoate depolymerase [Herbaspirillum seropedicae SmR1] Length = 408 Score = 731 bits (1888), Expect = 0.0, Method: Compositional matrix adjust. Identities = 359/408 (87%), Positives = 382/408 (93%) Query: 1 MINTYQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFARTQLTHA 60 MI TYQLYQNYADATDPLRACARMMA LGATWPG+PV+P RKMASACEVFARTQLTHA Sbjct: 1 MIPTYQLYQNYADATDPLRACARMMAQALGATWPGIPVHPYWRKMASACEVFARTQLTHA 60 Query: 61 RPPFEIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKESSAVQPKVLVVAPMSGHFATLLR 120 RPPF I V + G TI+VHEEE+ ATPFCGLLHFRK+S+ VQPKVLVVAPMSGHFATLLR Sbjct: 61 RPPFGIATVEEEGCTISVHEEEIHATPFCGLLHFRKDSATVQPKVLVVAPMSGHFATLLR 120 Query: 121 GTVKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLLAVCQPTV 180 GTV+TLLRDHDVYITDWRNARDVA HGRFGLDEYVSH+IDFL +LGPGAHLLAVCQPTV Sbjct: 121 GTVRTLLRDHDVYITDWRNARDVATAHGRFGLDEYVSHIIDFLGVLGPGAHLLAVCQPTV 180 Query: 181 AALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNMISTVPAR 240 AALTAA+VMA D HPAQPR++TLMAGP+DTRVNPTAVNALAKSKPI+WFEKNMISTVPAR Sbjct: 181 AALTAAAVMAADGHPAQPRSMTLMAGPIDTRVNPTAVNALAKSKPIAWFEKNMISTVPAR 240 Query: 241 HGGAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYEEYFAMAD 300 H GAGRRVYPGFVQL+AFMNMNL+RHV+AF KLYHHLV+GE A+A QIKDFYEEYFAMAD Sbjct: 241 HAGAGRRVYPGFVQLAAFMNMNLSRHVEAFGKLYHHLVDGEHARADQIKDFYEEYFAMAD 300 Query: 301 LPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICAVGQTVAA 360 LPAEFYLETVRTVFQEHALPLGKL+Y R VEPRAIRRTALFTIEGEKDDICAVGQT+AA Sbjct: 301 LPAEFYLETVRTVFQEHALPLGKLSYAGRPVEPRAIRRTALFTIEGEKDDICAVGQTLAA 360 Query: 361 QEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 QE+CS IRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR Sbjct: 361 QELCSGIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFINMHHR 408 >gi|300310951|ref|YP_003775043.1| poly[D(-)-3-hydroxyalkanoate] depolymerase [Herbaspirillum seropedicae SmR1] Length = 411 Score = 364 bits (935), Expect = e-102, Method: Compositional matrix adjust. Identities = 186/405 (45%), Positives = 255/405 (62%), Gaps = 6/405 (1%) Query: 5 YQLYQNYADATDPLRACARMMAPLLGATWPGVPVNPALRKMASACEVFARTQLTHARPPF 64 YQL++ +P+ A A L + P +++A+ E+ R + +P F Sbjct: 3 YQLHELNRAFLNPMMQWAETSAKLFSDPVSPLAHTPFSQRIAAGYELLYRLSKEYEKPQF 62 Query: 65 EIGEVLDGGRTIAVHEEEVSATPFCGLLHFRKESSA------VQPKVLVVAPMSGHFATL 118 I V G+++ + EE V PFC L+HFRK+ SA QP VLVVAP+SGH +TL Sbjct: 63 NIDAVPVDGKSVGIVEEVVEEKPFCRLIHFRKDLSARQATALKQPTVLVVAPLSGHHSTL 122 Query: 119 LRGTVKTLLRDHDVYITDWRNARDVAVEHGRFGLDEYVSHVIDFLDMLGPGAHLLAVCQP 178 LR TV+ LL++HDVYITDW +AR V VE G F L +YV +V DF+ LGP H+++VCQP Sbjct: 123 LRETVRALLQEHDVYITDWTDARMVPVEQGAFHLHDYVYYVQDFIRTLGPDVHVISVCQP 182 Query: 179 TVAALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTAVNALAKSKPISWFEKNMISTVP 238 TV L A S+MA N P P+++T+M GP+D R +PTAVN LA KP SWFE +I +VP Sbjct: 183 TVPVLAAISLMASANDPMLPKSMTMMGGPIDARKSPTAVNDLATEKPYSWFENTVIYSVP 242 Query: 239 ARHGGAGRRVYPGFVQLSAFMNMNLNRHVDAFRKLYHHLVEGEEAQAAQIKDFYEEYFAM 298 A + G GR+VYPGF+Q + F+ MN RH + Y HL +G++A A + + FY+EY A+ Sbjct: 243 ANYPGFGRKVYPGFLQHAGFVAMNPRRHAQSHWDFYMHLRDGDDASAEEHRKFYDEYNAV 302 Query: 299 ADLPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRAIRRTALFTIEGEKDDICAVGQTV 358 D+PAEFYLET++ VFQ+ L G ++V P+ I+ ALFTIEGE DDI GQT Sbjct: 303 LDMPAEFYLETIKVVFQDFNLARGTWEIEGQLVRPQDIKSVALFTIEGELDDISGSGQTQ 362 Query: 359 AAQEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEIYPRLRDFI 403 AAQE+CS+I + H GHYG+F+GRRW + P++ +FI Sbjct: 363 AAQELCSSIPKARKQHFTVPKAGHYGIFSGRRWREIVCPKIGEFI 407 >gi|300309607|ref|YP_003773699.1| poly(3-hydroxyalkanoate)synthetase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 83.6 bits (205), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 77/308 (25%), Positives = 139/308 (45%), Gaps = 34/308 (11%) Query: 106 LVVAPMSGHFATLL-----RGTVKTLLR---DHDVYITDWRNARDVAVEHGRFGLDEYVS 157 L+VAP +GH + + + V+TLL DH V + DW +A + + +D Y++ Sbjct: 89 LIVAPYAGHTSVIADYDQGQSLVETLLGNGVDH-VLLIDWHSA---SADMKDLEIDNYLA 144 Query: 158 HVIDFLDMLGPGAHLLAVCQPT-VAALTAASVMAEDNHPAQPRTLTLMAGPLDTRVNPTA 216 + +D LG L+ +CQ ++A+ AA P + L L P+D Sbjct: 145 DLCVVIDELGGRVRLVGLCQGGWISAMIAA------RFPEKVARLVLAGSPIDAGAGEGP 198 Query: 217 VNALAKSKPISWFEKNMISTVPARHGGAGRRVYPGFVQLSAFMNMNLNRH-VDAFRKLYH 275 + + + P+ ++E + R GG R G L + NM+ + H + +L Sbjct: 199 LKQMVQRLPLRFYEDLV------RSGGGLMR---GSFMLQGWKNMHPDEHYFNEHLELLQ 249 Query: 276 HLVEGEEAQAAQIKDFYEEYFAMADLPAEFYLETVRTVFQEHALPLGKLTYRERVVEPRA 335 H+ + A A+ + F Y DLP +YL+ + +F+++ L GK + ++ R Sbjct: 250 HI--DDPAYLAKRETFAAWYETTVDLPGRWYLQAIGHIFKDNLLARGKYVALGQQLDLRQ 307 Query: 336 IRRTALFTIEGEKDDICAVGQTVAAQEMCSNIRPYMRLHHVQTAVGHYGVFNGRRWDNEI 395 I L+ + GE+DDI Q + A + P ++ GH G+F G R E+ Sbjct: 308 I-TCPLYLLAGERDDITTPEQVLNAAALVGT--PAAQITQKIVPGGHIGLFMGSRTLAEV 364 Query: 396 YPRLRDFI 403 +P++ ++ Sbjct: 365 WPQIATWL 372 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,326,958 Number of extensions: 55624 Number of successful extensions: 221 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 3 Length of query: 408 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 321 Effective length of database: 1,207,925 Effective search space: 387743925 Effective search space used: 387743925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 132 (55.5 bits)