BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0014.0017.p
         (218 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300309973|ref|YP_003774065.1| glutathione S-transferase [Herb...   448   e-127
gi|300313568|ref|YP_003777660.1| glutathione S-transferase [Herb...   173   1e-44
gi|300313883|ref|YP_003777975.1| glutathione S-transferase [Herb...    56   2e-09

>gi|300309973|ref|YP_003774065.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 218

 Score =  448 bits (1152), Expect = e-127,   Method: Compositional matrix adjust.
 Identities = 216/218 (99%), Positives = 217/218 (99%)

Query: 1   MPKTTLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH 60
           MPKTTLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH
Sbjct: 1   MPKTTLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLH 60

Query: 61  HGDVLVWDTLAIAEYLNEIRPGAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG 120
           HGDVLVWDTLAIAEYLNEIRP AKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG
Sbjct: 61  HGDVLVWDTLAIAEYLNEIRPAAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKG 120

Query: 121 HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRTMADAMYAPVVTRFMTYDVKL 180
           HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRT+ADAMYAPVVTRFMTYDVKL
Sbjct: 121 HFPNFKVWSRAEADIVRITTIWKECLAKYGGPFLFGEQRTVADAMYAPVVTRFMTYDVKL 180

Query: 181 DPICTAYAKRIMAMPEMQEWITAAKKEPEDIDELDVEF 218
           DPICTAYAKRIMAMPEMQEWITAAKKEPEDIDELDVEF
Sbjct: 181 DPICTAYAKRIMAMPEMQEWITAAKKEPEDIDELDVEF 218


>gi|300313568|ref|YP_003777660.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 233

 Score =  173 bits (438), Expect = 1e-44,   Method: Compositional matrix adjust.
 Identities = 93/206 (45%), Positives = 126/206 (61%), Gaps = 2/206 (0%)

Query: 5   TLRITSKNYSSWSLRGWLMARFAGLSFEEDIVPPDDPSARAEILLLSSSILVPSLHHGDV 64
           TL I +KNYSSWS+R W+      + F+E  +  D     AEI   + S  VP L  GD+
Sbjct: 23  TLVIANKNYSSWSMRPWVAMVAFDIPFKEVRLLLDQADTAAEIARYTGSGRVPVLLDGDI 82

Query: 65  LVWDTLAIAEYLNEIRPGAKLLPADLAARAHCRSVCGEMHSGFSALRAALPMNLKGHFPN 124
            +WD+L+I EYL E  P   L PA   ARA  RS+C EMHSGF+ LR+A+ M+++G +P 
Sbjct: 83  AIWDSLSICEYLAEQFPDRHLWPARTDARAIARSICAEMHSGFTGLRSAMSMDIRGRYPG 142

Query: 125 FKVWSRAEADIVRITTIWKECLAKYG-GPFLFGEQRTMADAMYAPVVTRFMTYDVKLDPI 183
               + A+ADI R+  IW+ECL+++G   FLFG+  ++ADA YAPVV RF++Y V L P 
Sbjct: 143 QGRTAEAQADIARVCEIWEECLSQFGHHDFLFGD-FSIADAFYAPVVMRFISYQVPLAPA 201

Query: 184 CTAYAKRIMAMPEMQEWITAAKKEPE 209
             AYA R+   P +  WI  A  E E
Sbjct: 202 LQAYADRVREHPAVARWIAEALAEKE 227


>gi|300313883|ref|YP_003777975.1| glutathione S-transferase
           [Herbaspirillum seropedicae SmR1]
          Length = 225

 Score = 55.8 bits (133), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 46/152 (30%), Positives = 69/152 (45%), Gaps = 5/152 (3%)

Query: 56  VPSLHHGDVLVWDTLAIAEYLNEIRPGAKLLPADLAARAHCRSVCGEMHSGFSALRAALP 115
           VP L HG + + ++ AI EYL+E+  G +L P D  ARA  R V   + S   ALR    
Sbjct: 74  VPMLRHGALTLSESSAITEYLDEVFAGPRLYPQDAVARARARQVQAWLRSDLMALREE-- 131

Query: 116 MNLKGHFPNFKVWSRAEADIVRITTIWKEC--LAKYGGPFLFGEQRTMADAMYAPVVTRF 173
            N +  F   K  + +E        ++     L   G   LFG Q  +AD   A ++ R 
Sbjct: 132 RNTQVVFYGAKKAALSEEASAAAQRLFHFAGQLLPVGADSLFG-QWCIADVDLALMLNRL 190

Query: 174 MTYDVKLDPICTAYAKRIMAMPEMQEWITAAK 205
             +  ++ P    YA+R    P +Q W+T  +
Sbjct: 191 ALHGDRVPPHLEDYARRQWQRPSVQAWLTQPR 222


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.322    0.135    0.423 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 683,309
Number of extensions: 24604
Number of successful extensions: 63
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 62
Number of HSP's successfully gapped: 3
Length of query: 218
Length of database: 1,619,870
Length adjustment: 82
Effective length of query: 136
Effective length of database: 1,231,600
Effective search space: 167497600
Effective search space used: 167497600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 128 (53.9 bits)