BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0015.p (475 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309971|ref|YP_003774063.1| hypothetical protein Hsero_0636... 952 0.0 gi|300309976|ref|YP_003774068.1| hypothetical protein Hsero_0641... 278 9e-76 >gi|300309971|ref|YP_003774063.1| hypothetical protein Hsero_0636 [Herbaspirillum seropedicae SmR1] Length = 475 Score = 952 bits (2462), Expect = 0.0, Method: Compositional matrix adjust. Identities = 461/475 (97%), Positives = 466/475 (98%) Query: 1 MAHFFDEMYSGGAGSEAGQVRQHYAEFASWLSAQSPEIIARKRAESDLIFRRVGITFAVY 60 MAHFFDEMYSG + QVRQHYAEFA+WLSAQS E IARKRAESDLIFRRVGITFAVY Sbjct: 1 MAHFFDEMYSGESHGNEAQVRQHYAEFANWLSAQSAETIARKRAESDLIFRRVGITFAVY 60 Query: 61 GNDAGTERLIPFDIIPRIIHKAEWAKLEAGLVQRVKALNMFIHDIYHEQKIVKAGIIPPE 120 GNDAGTERLIPFDIIPRIIHKAEWA+LEAGLVQRV+ALNMFIHDIYHEQKIVKAGIIPPE Sbjct: 61 GNDAGTERLIPFDIIPRIIHKAEWARLEAGLVQRVRALNMFIHDIYHEQKIVKAGIIPPE 120 Query: 121 QIFQNAQYRREMQDVNVVNDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM 180 QIFQNAQYRREMQDVNVVNDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM Sbjct: 121 QIFQNAQYRREMQDVNVVNDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKM 180 Query: 181 MMRLFPDLFKRHKIAPVDHYPDLLLDNLRSVAPAGVTDPTVVVMTPGMYNSAYFEHAFLA 240 MMRLFPDLF RHKIAPVDHYPDLLLDNLRSVAPAGVTDPTVVVMTPGMYNSAYFEHAFLA Sbjct: 181 MMRLFPDLFTRHKIAPVDHYPDLLLDNLRSVAPAGVTDPTVVVMTPGMYNSAYFEHAFLA 240 Query: 241 QQMGVELVEGQDLFVKDNVVYMRTTRGPKRVDVIYRRIDDDFLDPLAFRPDSALGVPGLL 300 QQMGVELVEGQDLFVKDNVVYMRTTRGPKRVDVIYRRIDDDFLDPLAFRPDSALGVPGLL Sbjct: 241 QQMGVELVEGQDLFVKDNVVYMRTTRGPKRVDVIYRRIDDDFLDPLAFRPDSALGVPGLL 300 Query: 301 QAYRAGKVTLTNAIGTGVADDKSIYPYVPDMVRFYLSEDPILNNVPTYQCRKKEDLSYTL 360 QAYRAGKVTLTNAIGTGVADDKSIYPYVPDMVRFYLSEDPILNNVPTYQCRKKEDLSYTL Sbjct: 301 QAYRAGKVTLTNAIGTGVADDKSIYPYVPDMVRFYLSEDPILNNVPTYQCRKKEDLSYTL 360 Query: 361 ANLEKLVVKEVHGAGGYGMLVGPASTKQEIEDFRARIIAKPDGYIAQPTLALSVCPTYVE 420 ANLEKLVVKEVHGAGGYGMLVGPAST+QEIEDFRARIIAKPDGYIAQPTLALSVCPTYVE Sbjct: 361 ANLEKLVVKEVHGAGGYGMLVGPASTRQEIEDFRARIIAKPDGYIAQPTLALSVCPTYVE 420 Query: 421 SGIAPRHLDLRPFVLSGKQITMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK 475 SGIAPRHLDLRPFVLSGKQITMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK Sbjct: 421 SGIAPRHLDLRPFVLSGKQITMVKGGLTRVALKEGSLVVNSSQGGGTKDTWILEK 475 >gi|300309976|ref|YP_003774068.1| hypothetical protein Hsero_0641 [Herbaspirillum seropedicae SmR1] Length = 833 Score = 278 bits (710), Expect = 9e-76, Method: Compositional matrix adjust. Identities = 161/482 (33%), Positives = 254/482 (52%), Gaps = 22/482 (4%) Query: 5 FDEMYSGGAGSEAGQVRQHYAEFASWLSAQSPEIIARKRAESDLIFRRVGITFAVYGNDA 64 +DEM + G++R H+ L SP+++ R+ E G+T+ VY + Sbjct: 16 YDEMLAAD-----GRLRPHWRTLIDQLENLSPDMLRRRANEVRDAIASDGVTYNVYADPK 70 Query: 65 GTERLIPFDIIPRIIHKAEWAKLEAGLVQRVKALNMFIHDIYHEQKIVKAGIIPPEQIFQ 124 G R D++P ++ EW LE + QR + +N + DIY Q ++ G++PP +F Sbjct: 71 GANRPWELDLLPHLVSSEEWDFLERAVGQRARLMNGLLSDIYGPQTLIAEGLLPPALVFG 130 Query: 125 NAQYRREMQDVNVVNDIYAHIAGVDIVRAGEGEFYVLEDNLRVPSGVSYMLENRKMMMRL 184 Y ++ H +D+ R+ +G ++V+ D + PSG Y L+NR++M R Sbjct: 131 QHGYLMPCHGWQPPGGVHLHAYAIDLARSVDGTWWVVSDRTQGPSGTGYALQNRQIMARA 190 Query: 185 FPDLFKRHKIAPVDHYPDLLLDNLRSVAPAGVTDPTVVVMTPGMYNSAYFEHAFLAQQMG 244 P+ + + Y L L +APA P +V++TPG YN Y EHAFLA +G Sbjct: 191 MPEALRDMHVESPHRYFHTLRAMLTRLAPAQGEVPLIVLLTPGPYNETYSEHAFLAHTLG 250 Query: 245 VELVEGQDLFVKDNVVYMRTTRGPKRVDVIYRRIDDDFLDPLAFRPDSALGVPGLLQAYR 304 LVEG DL V+ V+++T G +RV I RR+DDD+ DPL R DSALGVPGL QA R Sbjct: 251 FPLVEGVDLTVRGEQVFLKTLNGLRRVHAILRRMDDDYCDPLELRADSALGVPGLTQAAR 310 Query: 305 AGKVTLTNAIGTGVADDKSIYPYVPDMVRFYLSEDPILNNVPTYQCRKKEDLSYTLANLE 364 G V + N++G+GV + +++ ++P + E +L +V ++ C +K L YTLA+ + Sbjct: 311 LGNVLIANSLGSGVLESTALHGFLPAINERLYGEPLLLPSVASWWCGEKPALDYTLAHFD 370 Query: 365 KLVV----KEVHGAGGYGMLVGPASTKQEIEDFRARIIAKPDGYIAQPTLALSVCPTYVE 420 +LV+ + +G V A+ ++ IE + +P Y AQ + LS P Sbjct: 371 ELVIVPAFSSMRMQPVFGHSVTGAARRRLIESLQ----LQPHAYAAQEWVRLSQAPVMSR 426 Query: 421 SG---IAPRHLDLRPFVLS----GKQITMVKGGLTRVALKEGSLVVNSSQGGGTKDTWIL 473 SG + R + LR F ++ G Q+ + GGLTRVA ++ VV+ QGG +KD W+L Sbjct: 427 SGDYRLMNRTISLRVFAVADGEGGYQV--MPGGLTRVAPRQRRDVVSMQQGGTSKDVWVL 484 Query: 474 EK 475 + Sbjct: 485 RE 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.139 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,533,057 Number of extensions: 64356 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 2 Number of HSP's gapped: 147 Number of HSP's successfully gapped: 2 Length of query: 475 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 386 Effective length of database: 1,198,455 Effective search space: 462603630 Effective search space used: 462603630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)