BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0008.p (161 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309963|ref|YP_003774055.1| 2-amino-4-hydroxy-6-hydroxymeth... 265 1e-72 >gi|300309963|ref|YP_003774055.1| 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophosphokinase [Herbaspirillum seropedicae SmR1] Length = 161 Score = 265 bits (678), Expect = 1e-72, Method: Compositional matrix adjust. Identities = 130/161 (80%), Positives = 145/161 (90%) Query: 1 VESSSTLAWIGIGGNLGDARATVEEAIAHLARLPESQLLRSSSLYRTAPIDSSGDDYVNA 60 +E +STLAWIGIGGNLGDARATVE AIA LA LP++QLLRSSSLYRTAP+D+SGDDYVNA Sbjct: 1 MEPASTLAWIGIGGNLGDARATVETAIARLAALPQTQLLRSSSLYRTAPVDASGDDYVNA 60 Query: 61 VALVSTSLSALELLHALQAIELQHGRERPYRNAPRTLDLDVLMYGEAQLEDEKLIVPHPR 120 VALVST+L AL+LLHALQAIELQHGRERPYRNAPRTLDLDVLMYGEA++ D++L VPHPR Sbjct: 61 VALVSTTLPALDLLHALQAIELQHGRERPYRNAPRTLDLDVLMYGEARIADQELTVPHPR 120 Query: 121 MAQRAFVLVPMLEIDAQVRIPGLGAARDFLDAITDQPISRL 161 M QRAFVL P+LEID IPG+GAAR LDAITDQPI R+ Sbjct: 121 MVQRAFVLAPLLEIDENAVIPGVGAARPLLDAITDQPIRRI 161 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 502,172 Number of extensions: 18996 Number of successful extensions: 60 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 60 Number of HSP's successfully gapped: 1 Length of query: 161 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 83 Effective length of database: 1,250,540 Effective search space: 103794820 Effective search space used: 103794820 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)