BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0006.p (220 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 351 3e-98 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 186 1e-48 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 113 1e-26 gi|300312321|ref|YP_003776413.1| protein-glutamate methylesteras... 110 8e-26 gi|300312675|ref|YP_003776767.1| protein-glutamate methylesteras... 80 2e-16 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 351 bits (900), Expect = 3e-98, Method: Compositional matrix adjust. Identities = 172/198 (86%), Positives = 181/198 (91%) Query: 1 MLHDDGWAAVLQRHTADAVLAAPGPEDGYRPLTARIVAIGASTGGPQALERVLKPLTGRC 60 MLHD W AVLQR+TADAVL PGPEDG RP TARIVAIGASTGGPQALERVL L+ RC Sbjct: 161 MLHDAAWGAVLQRYTADAVLQPPGPEDGLRPATARIVAIGASTGGPQALERVLGKLSQRC 220 Query: 61 PGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHHDLVLPGRVLIAPGGRHLVLRRDGQQ 120 PGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEH D +LPGRVLIAPGGRHLV+RR+G Q Sbjct: 221 PGIVVVQHMPQRFTRSFAERLHRISGAEVKEAEHLDRILPGRVLIAPGGRHLVVRREGLQ 280 Query: 121 YYVETLDGPLVSRHKPSVDVLFRSAAKAAGSNAVGIIMTGMGDDGARGMREMADCGAATY 180 YYVETLDGPLVSRHKPSVDVLFRS AKAAGSNAVGIIMTGMGDDGARGMRE+ADCG ATY Sbjct: 281 YYVETLDGPLVSRHKPSVDVLFRSLAKAAGSNAVGIIMTGMGDDGARGMRELADCGGATY 340 Query: 181 AQDEASSVVFGMPRWSVH 198 AQDEASSVVFGMP+ ++ Sbjct: 341 AQDEASSVVFGMPKEAIQ 358 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 186 bits (473), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 93/172 (54%), Positives = 121/172 (70%), Gaps = 2/172 (1%) Query: 30 RPLTA--RIVAIGASTGGPQALERVLKPLTGRCPGIVVVQHMPQRFTRSFAERLHRISGA 87 PLT+ +++ IGASTGG +A++ L + CPGI++ QHMP+ FTRSFA+RL+ + Sbjct: 158 NPLTSSEKLIIIGASTGGTEAIKEFLIRMPSDCPGILITQHMPEGFTRSFAQRLNNLCKI 217 Query: 88 EVKEAEHHDLVLPGRVLIAPGGRHLVLRRDGQQYYVETLDGPLVSRHKPSVDVLFRSAAK 147 V E+ ++ VLPG IAPG HL L R G Y + GP V+RH+PSVDVLF+SAA Sbjct: 218 SVVESAGNERVLPGHAYIAPGHSHLKLVRSGANYMTQLDQGPPVNRHRPSVDVLFQSAAT 277 Query: 148 AAGSNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPRWSVHV 199 AG NAVG+I+TGMG DGA+GM EM + GA +AQDEAS VVFGMPR ++ V Sbjct: 278 CAGKNAVGVILTGMGKDGAQGMLEMRNAGAYNFAQDEASCVVFGMPREAIAV 329 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 113 bits (282), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 81/205 (39%), Positives = 108/205 (52%), Gaps = 9/205 (4%) Query: 20 LAAPGPEDGYR--PLTARIVAIGASTGGPQALERVLKPLTGRCP-GIVVVQHMPQRFTRS 76 LAAP E R P + +V IG STGGP LE +L L P I+V QHMP FTRS Sbjct: 148 LAAPVAEAARRGRPASDGLVLIGVSTGGPSTLEEILPALPASFPWPILVAQHMPAAFTRS 207 Query: 77 FAERLHRISGAEVKEAEHHDLVLPGRVLIAPGGRHLVLRRDGQQYYVETLDGPLVSRH-- 134 FAER+ ++ V E +V G V IA GG +++ + G V L P H Sbjct: 208 FAERMDKLCALNVLEVAAPTVVQGGHVYIARGGADMLVAKRGSHLSV--LPKPESPTHLW 265 Query: 135 KPSVDVLFRSAAKA-AGSNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMP 193 PSV++L RSA ++ A +G+++TGMG+DGA E+ G T A+ E+S VVFGMP Sbjct: 266 HPSVELLARSALQSMAARQLIGVMLTGMGNDGAAAFAEICGDGGRTIAESESSCVVFGMP 325 Query: 194 RWSVHV-STTVPCSASTACRCCRTW 217 + + +V AS R W Sbjct: 326 KDLIDRGGASVVLPASKIATQLRVW 350 >gi|300312321|ref|YP_003776413.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 110 bits (276), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 71/184 (38%), Positives = 101/184 (54%), Gaps = 8/184 (4%) Query: 21 AAPGPEDGYRPLTARIVAIGASTGGPQALERVLKPL-TGRCPGIVVVQHMPQRFTRSFAE 79 A P D PL VA+GAS GGP AL +LK + G +V+VQH+ F A+ Sbjct: 149 AQPARADSLPPL----VAVGASAGGPAALATLLKAIPQGFAGAVVIVQHIDAAFAPGMAQ 204 Query: 80 RLHRISGAEVKEAEHHDLVLPGRVLIAPGGRHLVLRRDGQQYYVETLDGPLVSRHKPSVD 139 L + S +V A+ G VL+A RHLV++ G+ YVE G ++PSVD Sbjct: 205 WLQQQSALKVGLAQEGQAPQRGAVLLAASNRHLVVKEGGRLGYVE---GAPNDVYRPSVD 261 Query: 140 VLFRSAAKAAGSNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPRWSVHV 199 V F+S + G+++TGMG DGA G++ + D GA T AQD+ S V+GMP+ +V + Sbjct: 262 VFFQSIVRHWPGKVAGLLLTGMGRDGAAGLKALRDSGAHTMAQDQKSCAVYGMPKAAVAL 321 Query: 200 STTV 203 + V Sbjct: 322 NAAV 325 >gi|300312675|ref|YP_003776767.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 211 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 60/180 (33%), Positives = 89/180 (49%), Gaps = 11/180 (6%) Query: 23 PGPEDGYRPLTARIVAIGASTGGPQALERVLKPLT-GRCPGIVVVQHMPQR----FTRSF 77 P PE RP+ A + IGAS GG +AL +L L G +VV H+P+ + F Sbjct: 5 PLPELAARPIEA--IVIGASAGGVEALSEILPALRRGAAVATMVVLHLPRERPSLLAQIF 62 Query: 78 AERLHRISGAEVKEAEHHDLVLPGRVLIAPGGRHLVLRRDGQQYYVETLDGPLVSRHKPS 137 A+R + V EA+ + G + AP HL++ R + + LV+ +P+ Sbjct: 63 ADRCRQ----PVVEAQDKEPAQAGVIHFAPPDYHLLVDRSEDGPILAMSNDELVNYSRPA 118 Query: 138 VDVLFRSAAKAAGSNAVGIIMTGMGDDGARGMREMADCGAATYAQDEASSVVFGMPRWSV 197 +DVLF SAA G +GI++TG DGA GM+ +A G T Q + V MP ++ Sbjct: 119 IDVLFESAADVYGPRLLGILLTGGNHDGAAGMQAIARAGGVTVVQSPQQAQVPYMPAQAL 178 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 788,346 Number of extensions: 34843 Number of successful extensions: 108 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 5 Length of query: 220 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 138 Effective length of database: 1,231,600 Effective search space: 169960800 Effective search space used: 169960800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)