BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0005.p (157 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylester... 177 5e-46 gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylester... 105 2e-24 gi|300311029|ref|YP_003775121.1| protein-glutamate methylesteras... 66 1e-12 gi|300312077|ref|YP_003776169.1| response regulator containing a... 60 6e-11 gi|300310532|ref|YP_003774624.1| virulence regulator protein [He... 59 1e-10 >gi|300309961|ref|YP_003774053.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 381 Score = 177 bits (448), Expect = 5e-46, Method: Compositional matrix adjust. Identities = 106/136 (77%), Positives = 121/136 (88%) Query: 1 MNAPSAIRVMVVDDSSVVRQLVQQLLAQTADIRVIGTAPDPVFAMRKMQQEWPDVILLDI 60 M+ P+ IRVMVVDDSSVVRQL+QQ+LAQT DIRVIGTAPDPVFA+RKMQQ+WPDVI+LDI Sbjct: 1 MSTPAMIRVMVVDDSSVVRQLMQQVLAQTPDIRVIGTAPDPVFALRKMQQQWPDVIVLDI 60 Query: 61 EMPRMDGITFLRQIMASRPTPVIMCSARVGGAGDITAEALAAGAVSVIARPQLGVRDFLH 120 +MPRMDG++FLRQIMA+RPTPVIMCSA VG + AEALAAGAVSVIARPQLGVRDFL Sbjct: 61 KMPRMDGVSFLRQIMATRPTPVIMCSAHVGPDNALAAEALAAGAVSVIARPQLGVRDFLQ 120 Query: 121 EAVTELACAILESLSA 136 +AV ELA AI ++ A Sbjct: 121 QAVEELASAIRAAMQA 136 >gi|300311338|ref|YP_003775430.1| chemotaxis-specific methylesterase [Herbaspirillum seropedicae SmR1] Length = 359 Score = 105 bits (262), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 47/124 (37%), Positives = 81/124 (65%) Query: 7 IRVMVVDDSSVVRQLVQQLLAQTADIRVIGTAPDPVFAMRKMQQEWPDVILLDIEMPRMD 66 I+V++VDDS+++R ++++++ D+ V+G APDP+ A ++Q PDV+ LD+EMPRMD Sbjct: 3 IKVLIVDDSALIRSVMREIIGSQPDMEVVGVAPDPIIARELIKQTNPDVLTLDVEMPRMD 62 Query: 67 GITFLRQIMASRPTPVIMCSARVGGAGDITAEALAAGAVSVIARPQLGVRDFLHEAVTEL 126 G+ FL ++M RP PV+M S+ +IT AL GA+ + +P++ ++ + E + Sbjct: 63 GLDFLEKLMRLRPMPVVMVSSLTERGSEITLRALELGAIDFVTKPKISIQSGMQEYADMI 122 Query: 127 ACAI 130 A I Sbjct: 123 ADKI 126 >gi|300311029|ref|YP_003775121.1| protein-glutamate methylesterase [Herbaspirillum seropedicae SmR1] Length = 353 Score = 66.2 bits (160), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 41/117 (35%), Positives = 64/117 (54%), Gaps = 5/117 (4%) Query: 7 IRVMVVDDSSVVRQLVQQLLAQTA--DIRVIGTAPDPVFAMRKMQQEWPDVILLDIEMPR 64 I+++++DDS+++R+ + Q+ Q DI + + V R PDV+ LDI MP Sbjct: 2 IKLLIIDDSALMRRQLMQVFEQAGGFDICLGRNGREAVELNRSFA---PDVVTLDINMPE 58 Query: 65 MDGITFLRQIMASRPTPVIMCSARVGGAGDITAEALAAGAVSVIARPQLGVRDFLHE 121 MDG++ L +M RP PV+M S+ T EA+ GAV +A+P + LHE Sbjct: 59 MDGLSALSLMMLDRPVPVVMVSSLTEQGALATFEAMNLGAVDYVAKPSGTISLSLHE 115 >gi|300312077|ref|YP_003776169.1| response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 217 Score = 60.5 bits (145), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 36/107 (33%), Positives = 63/107 (58%), Gaps = 3/107 (2%) Query: 5 SAIRVMVVDDSSVVRQLVQQLLAQTADIRVIGTAPDPVFAMRKMQQEWPDVILLDIEMPR 64 +AI+V++ DD ++VR+ ++Q+ A + DI V G A + + A++ ++ DV+LLDI +P Sbjct: 7 AAIKVLIADDHAIVREGLRQICAGSKDIMVAGNAANGLEAIKLAREGNGDVLLLDIVLPD 66 Query: 65 MDGITFLRQIMASRPT-PVIMCSARVGGAGDITAEALAAGAVSVIAR 110 +GI L+QI +P PV++ S + AL AGA + + Sbjct: 67 RNGIDILKQIRKEQPELPVLILS--IHREDQYAIRALKAGAAGFLNK 111 >gi|300310532|ref|YP_003774624.1| virulence regulator protein [Herbaspirillum seropedicae SmR1] Length = 209 Score = 59.3 bits (142), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 43/132 (32%), Positives = 67/132 (50%), Gaps = 15/132 (11%) Query: 8 RVMVVDDSSVVRQLVQQLLAQTADIRVIGTAPDPVFAMRKMQ-QEWPDVILLDIEMPRMD 66 +++VVDD +VVR V +++ + + G A A+R ++ Q+W D++LLDI MP Sbjct: 3 KILVVDDHAVVRAGVHHFISEIPSMEIGGEASTAEEAIRLVRTQDW-DIVLLDIAMPDKS 61 Query: 67 GITFLRQIMASRPT-PVIMCSA-----------RVGGAGDITAEALAAGAVSVIARPQLG 114 G+ L+QI +P PV+M S R G +G + EALA V+ I G Sbjct: 62 GVEVLKQIKREKPELPVLMLSMHPESRYAVQVLRSGASGYVQKEALATELVNAINTILRG 121 Query: 115 VRDFLHEAVTEL 126 ++ V EL Sbjct: 122 -HKYISYGVAEL 132 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.132 0.371 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 369,269 Number of extensions: 12164 Number of successful extensions: 126 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 143 Number of HSP's successfully gapped: 9 Length of query: 157 Length of database: 1,619,870 Length adjustment: 78 Effective length of query: 79 Effective length of database: 1,250,540 Effective search space: 98792660 Effective search space used: 98792660 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 126 (53.1 bits)