BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0014.0001.p (498 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [He... 820 0.0 gi|300312286|ref|YP_003776378.1| signal-transducing histidine ki... 306 3e-84 gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [He... 239 5e-64 gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbas... 222 4e-59 gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Her... 181 8e-47 >gi|300309957|ref|YP_003774049.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 725 Score = 820 bits (2119), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/525 (81%), Positives = 453/525 (86%), Gaps = 29/525 (5%) Query: 1 VEADIPEAAAMDAESCYLGVEICLASDSTRQTLEDVFEFVRDDSRIDILPPQSPLAAYAT 60 VE+ IPEAAAMDAESCYLG+E+CLASDST QTLE+VFEFVRDDSRIDILPP++PL+AYA Sbjct: 203 VESAIPEAAAMDAESCYLGMEVCLASDSTLQTLENVFEFVRDDSRIDILPPRAPLSAYAE 262 Query: 61 VLQRLAPDDAARLAAQWRSLDLFNEAQWRCLGAA--TLADAAAPAV-PAAPGGGGGR--- 114 L RLAP DAARLAA WR L LF++ QW+ L AA T +DA A AV P A G R Sbjct: 263 RLARLAPQDAARLAAAWRGLALFDQTQWQSLEAASATPSDALAAAVEPMAAEGVLQRPRA 322 Query: 115 ---------------------XIDLVGELVIAGASARLIARRRKDSQFEEATQAIDSLME 153 IDLVGELVIAGASARLIARRRKDSQFEEATQAID+LME Sbjct: 323 LDERRAQEQKFIKIEVSKLDQLIDLVGELVIAGASARLIARRRKDSQFEEATQAIDTLME 382 Query: 154 QIRDAALTLRMVQINEVFQRFPRIVRDIARDLDKDIDLTMTGSETELDKSMVERLADPLM 213 QIRDAALTLRMVQINEVFQRFPRIVRDIARDL KDIDL MTGS+TELDKSMVERLADPLM Sbjct: 383 QIRDAALTLRMVQINEVFQRFPRIVRDIARDLGKDIDLVMTGSDTELDKSMVERLADPLM 442 Query: 214 HIVRNAIDHGIEPAAERIAAGKPAKASLRLNAMHESGSVLVEVMDDGRGMDRTRILEKAI 273 HIVRNAIDHGIEPAAER+AAGKPAKASL+LNA+HESGSV++EVMDDGRGMDR IL KA+ Sbjct: 443 HIVRNAIDHGIEPAAERLAAGKPAKASLQLNAIHESGSVVIEVMDDGRGMDREAILAKAV 502 Query: 274 AQGLTTPEAELSDAEIYRFVFEPGFSTAEQVTELSGRGVGMDVVKRNIDALQGEVAIDTE 333 AQGL +P+AELSDAEIYRFVFEPGFSTA QVTELSGRGVGMDVV+RNI+AL GEV+IDT Sbjct: 503 AQGLASPDAELSDAEIYRFVFEPGFSTARQVTELSGRGVGMDVVRRNIEALHGEVSIDTG 562 Query: 334 PGRGTVVRIRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPRQNGSHIVAVRGEPL 393 GRGTVVR+RLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPP+Q GSHIV VR EPL Sbjct: 563 RGRGTVVRLRLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPQQPGSHIVNVRDEPL 622 Query: 394 PFVPLRELFDLPAREQARARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKG 453 PFVPLRELFDLPARE R R SLVIVQYG LRAGLLVDGLLGECQAVIKPLGRLFGKVKG Sbjct: 623 PFVPLRELFDLPARE--RGRNSLVIVQYGPLRAGLLVDGLLGECQAVIKPLGRLFGKVKG 680 Query: 454 LSGSTILGDGRVALILDIPHLVQHTQQQEQGNTAAYAERSVASAP 498 LSGSTILGDGRVALILDI HLV HTQQQEQG+ AYAER VASAP Sbjct: 681 LSGSTILGDGRVALILDIAHLVHHTQQQEQGSAQAYAERGVASAP 725 >gi|300312286|ref|YP_003776378.1| signal-transducing histidine kinase CheA protein [Herbaspirillum seropedicae SmR1] Length = 697 Score = 306 bits (783), Expect = 3e-84, Method: Compositional matrix adjust. Identities = 164/373 (43%), Positives = 240/373 (64%), Gaps = 9/373 (2%) Query: 116 IDLVGELVIAGASARLIARRRKDSQFEEATQAIDSLMEQIRD---AALTLRMVQINEVFQ 172 I+LVGELVI A + E ++ L RD + +++RM+ ++ VF Sbjct: 318 INLVGELVITQAMIEQRVGKLDPMTHEHLLNSVSQLSRNTRDLQESVMSIRMMPMDYVFS 377 Query: 173 RFPRIVRDIARDLDKDIDLTMTGSETELDKSMVERLADPLMHIVRNAIDHGIEPAAERIA 232 RFPR+VRD+A L K ++ G+ TELDK ++ER+ DPL H+VRN+IDHGIE R A Sbjct: 378 RFPRMVRDLAHKLGKQVEFVTHGAATELDKGLIERIVDPLTHLVRNSIDHGIELPDARRA 437 Query: 233 AGKPAKASLRLNAMHESGSVLVEVMDDGRGMDRTRILEKAIAQGLTTPEAELSDAEIYRF 292 +GK +L L+A H+ G++++EV DDG G++R +IL KA QG+ + LSDAE+++ Sbjct: 438 SGKSEAGTLSLSAEHQGGNIIIEVTDDGGGLNREKILAKAKQQGMPVTD-NLSDAEVWQL 496 Query: 293 VFEPGFSTAEQVTELSGRGVGMDVVKRNIDALQGEVAIDTEPGRGTVVRIRLPLTLAIIS 352 +FEPGFSTAEQVT++SGRGVGMDVVKRNI A+ G V I + G GT + I LPLTLAI+ Sbjct: 497 IFEPGFSTAEQVTDVSGRGVGMDVVKRNILAMGGAVDIRSSKGFGTTISISLPLTLAILD 556 Query: 353 GFQVVVGNAVFVIPLDMVVECI-----DMPPRQNGSHIVAVRGEPLPFVPLRELFDLPAR 407 G + +G V+++PL VVE + D+ ++ VRGE LP +PL ++F++ Sbjct: 557 GMSIRIGEEVYILPLGYVVESLQPLAQDIKEISGQGQVLRVRGEYLPLIPLYQIFNIEPS 616 Query: 408 EQARARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILGDGRVAL 467 ++ +VI++ +A L VD L+G+ Q V+K L + KV G+SG+TILGDG VAL Sbjct: 617 FTDPSQGLVVILESDGKKAALFVDDLIGQQQIVVKNLESNYRKVAGISGATILGDGGVAL 676 Query: 468 ILDIPHLVQHTQQ 480 I+D+ L++ ++Q Sbjct: 677 IVDVAALLRSSRQ 689 >gi|300311033|ref|YP_003775125.1| chemotaxis histidine kinase [Herbaspirillum seropedicae SmR1] Length = 837 Score = 239 bits (609), Expect = 5e-64, Method: Compositional matrix adjust. Identities = 136/374 (36%), Positives = 228/374 (60%), Gaps = 18/374 (4%) Query: 114 RXIDLVGELVIAGASARLIARRRKDS--------QFEEATQAIDSLMEQIRDAALTLRMV 165 R ++L+GE+V++ + +A+R +D + + I+ + E+++DA + +RM+ Sbjct: 460 RLMNLIGEMVVSKNALPYLAQRAEDQFGVRELAREIKAQYAVINRIAEEMQDAIMQVRMM 519 Query: 166 QINEVFQRFPRIVRDIARDLDKDIDLTMTGSETELDKSMVERLADPLMHIVRNAIDHGIE 225 ++ VFQRFPR+VRDI+R L K++ L + G ETE DK+++E LADPL+HIVRN++DHG+E Sbjct: 520 PVSVVFQRFPRLVRDISRKLGKEVQLVLVGEETEADKNIIEALADPLIHIVRNSLDHGLE 579 Query: 226 PAAERIAAGKPAKASLRLNAMHESGSVLVEVMDDGRGMDRTRILEKAIAQGLTTPEAEL- 284 +R A+GKPA SL ++A HE V++ + DDG+G+D I KA +GL EA+L Sbjct: 580 GPEQRRASGKPALGSLTISAAHEGDQVVIVIEDDGKGIDPALIKRKAYEKGLID-EAQLE 638 Query: 285 --SDAEIYRFVFEPGFSTAEQVTELSGRGVGMDVVKRNIDALQGEVAIDTEPGRGTVVRI 342 SD + VF GFSTAE V++LSGRGVGMDVV+ ++ + G +A+ + G+GT ++I Sbjct: 639 RISDQDAVNLVFSSGFSTAEVVSDLSGRGVGMDVVRNAVEKVNGSLALHSVSGKGTRLQI 698 Query: 343 RLPLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPRQ----NGSHIVAVRGEPLPFVPL 398 LPL++A+ + +F +P++ V+E + +P N S + +RG L + Sbjct: 699 SLPLSMAVTKVMIIESDGQLFGVPMEHVMETVRVPRSSLHFANSSAKMVLRGHILGLKGI 758 Query: 399 RELFDLPAREQARA--RKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLSG 456 ++ + A ++ A ++++V+ G GL++D + KPL + G + SG Sbjct: 759 NQILGIAAEQKTNADGEVAVLVVRVGGQSVGLVIDDFRETVDVIQKPLLGILGGLSLYSG 818 Query: 457 STILGDGRVALILD 470 S ++GDG V ++L+ Sbjct: 819 SALMGDGSVLMVLN 832 >gi|300312396|ref|YP_003776488.1| chemotaxis CheA protein [Herbaspirillum seropedicae SmR1] Length = 638 Score = 222 bits (566), Expect = 4e-59, Method: Compositional matrix adjust. Identities = 133/382 (34%), Positives = 218/382 (57%), Gaps = 23/382 (6%) Query: 116 IDLVGELVIAGASARLIARRRKDSQFEEATQAIDSLM-----------EQIRDAALTLRM 164 +++ GE V A A ++ + + +FE A+ S + +++ A L+ RM Sbjct: 262 LEVAGESVQAANQAAVLLEKLQQFKFEGTAAALMSTLAETLGRASRYSSELQRATLSTRM 321 Query: 165 VQINEVFQRFPRIVRDIARDLDKDIDLTMTGSETELDKSMVERLADPLMHIVRNAIDHGI 224 + +FQ+FPR+VR++A+DL KD+DL +TG+ETE+D+ +V+ L DPL+H++RN++DHGI Sbjct: 322 QPVGRLFQKFPRLVRELAKDLGKDVDLVITGAETEVDRVVVDSLYDPLVHMLRNSLDHGI 381 Query: 225 EPAAERIAAGKPAKASLRLNAMHESGSVLVEVMDDGRGMDRTRILEKAIAQGLTTPEAEL 284 E +R+AAGKP KA++ LNA SV++EV DDG+GMD + KA+A+GL L Sbjct: 382 E--VDRVAAGKPTKATISLNAWQAGSSVMIEVFDDGKGMDPDMLRSKALAKGLINEHQAL 439 Query: 285 SDAEIYRFVFEPGFSTAEQVTELSGRGVGMDVVKRNIDALQGEVAIDTEPGRGTVVRIRL 344 + E + VF PGFST E + +SGRGVGMDVVK ++ +G + I++ G+GT IRL Sbjct: 440 TADEALQLVFLPGFSTKEVASSVSGRGVGMDVVKTAVEKHRGTIRIESTIGKGTKFSIRL 499 Query: 345 PLTLAIISGFQVVVGNAVFVIPLDMVVECIDMPPRQN---GSHIVAVRGEPLPFVPLREL 401 P+ L+II V A +P+ +V +++P + G+ ++ +G PL P+ L Sbjct: 500 PIELSIIPTMLVRCAGAPLALPMAVVERVVELPENFDEVGGAPVLRDQGRPL---PVNSL 556 Query: 402 FDLPAREQARARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLSGSTILG 461 + E R +V+ G V+ + G VIKPL + G++G+ Sbjct: 557 AGVLGYEPGNERVGIVMAVPHPYILG--VESVEGTADLVIKPLTAI--HTAGITGTARSA 612 Query: 462 DGRVALILDIPHLVQHTQQQEQ 483 +G + L++ + L+ + E+ Sbjct: 613 EGELVLVVGLSFLLDGCRDMER 634 >gi|300313133|ref|YP_003777225.1| CheA-like histidine kinase [Herbaspirillum seropedicae SmR1] Length = 744 Score = 181 bits (460), Expect = 8e-47, Method: Compositional matrix adjust. Identities = 113/315 (35%), Positives = 177/315 (56%), Gaps = 9/315 (2%) Query: 163 RMVQINEVFQRFPRIVRDIARDLDKDIDLTMTGSETELDKSMVERLADPLMHIVRNAIDH 222 RMV+ N + R +VR +A + K + L ++ ++D++++ERL PL H++RNA H Sbjct: 412 RMVKFNSMEVRMQHLVRQVAAETGKPLALDLSNCRLQIDRAILERLMGPLEHLLRNAAVH 471 Query: 223 GIEPAAERIAAGKPAKASLRLNAMHESGSVLVEVMDDGRGMDRTRILEKAIAQGLTTPEA 282 GIE A +R+A GKPA L + A HE ++ V DDGRG+D I +KA+ G A Sbjct: 472 GIESAEQRLAEGKPAVGRLTVQATHEGNEAVIRVSDDGRGLDLEAIRQKALELG-AAGLA 530 Query: 283 ELSDAEIYRFVFEPGFSTAEQVTELSGRGVGMDVVKRNIDALQGEVAIDTEPGRGTVVRI 342 E S A + +F+PG ST+ QV+ L+GRG+GMDVV+ + AL G + + ++PG+G + Sbjct: 531 EASQARLADLIFQPGLSTSPQVSALAGRGIGMDVVRSEVAALGGWMRVHSQPGQGVQFTL 590 Query: 343 RLPLTLAIISGFQVVVGNAVFVIP---LDMVVECIDMPPRQN-GSHIVAVRGEPLPFVPL 398 LPL+LA+ + G F IP +D +V+ RQ + +G L P Sbjct: 591 YLPLSLAVQHVCLLRHGQQHFAIPSMLVDSIVQLRGPQARQALAERSLEHQGSRLALHPW 650 Query: 399 RELFD---LPAREQARARKSLVIVQYGQLRAGLLVDGLLGECQAVIKPLGRLFGKVKGLS 455 L D PA E + + L+I + L A +L D + G + V+KP+G + G+ Sbjct: 651 HCLLDEAPEPAGEGSDSAYVLLIKRDDGLMA-VLADHVSGNREVVVKPVGPQLATLAGVV 709 Query: 456 GSTILGDGRVALILD 470 G+T+LGDG++ LI++ Sbjct: 710 GATVLGDGQIVLIIN 724 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,420,772 Number of extensions: 60245 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 310 Number of HSP's successfully gapped: 7 Length of query: 498 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 409 Effective length of database: 1,198,455 Effective search space: 490168095 Effective search space used: 490168095 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)