BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0026.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein... 462 e-131 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 105 5e-24 gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum s... 100 1e-22 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 94 1e-20 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 89 4e-19 >gi|300312114|ref|YP_003776206.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 462 bits (1190), Expect = e-131, Method: Compositional matrix adjust. Identities = 240/262 (91%), Positives = 252/262 (96%) Query: 1 MDLGIKGKTALVCASSKGLGRACALSLAREGVHVTLLARGAEALEATAQAIRTETAATGA 60 MDL + GKTALVCASSKGLGRACA SLAREGVHVT+LARG EALEA AQ IR E AATGA Sbjct: 1 MDLRLNGKTALVCASSKGLGRACAHSLAREGVHVTMLARGREALEAAAQEIRAEVAATGA 60 Query: 61 IITTVACDITTPEGRALALAACPAPDILVTNAGGPKPGDFREWTRDDWIAAVDANMLTPI 120 IITTV+CDITTPEGRALALAACPAPDILVTNAGGPKPGDFR+W+R+DWIAAVDANMLTPI Sbjct: 61 IITTVSCDITTPEGRALALAACPAPDILVTNAGGPKPGDFRDWSREDWIAAVDANMLTPI 120 Query: 121 ELIKATVDGMIACGFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 ELIKATVDGMIA FGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT Sbjct: 121 ELIKATVDGMIARRFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARKTVAHNVT 180 Query: 181 INNLLPGPFETDRLRSTATGAAKESGRTVDEVLADRRKLNPAGRFGDPAEFGDACAYLCS 240 INNLLPGPFETDRLR+TA+GAA+ESGR+VDE+LA+RRKLNPAGRFGDPAEFGDACAYLCS Sbjct: 181 INNLLPGPFETDRLRATASGAAQESGRSVDEILAERRKLNPAGRFGDPAEFGDACAYLCS 240 Query: 241 AQAGFITGQNLLMDGGAYPGTF 262 AQAGFITGQNLLMDGGAYPGTF Sbjct: 241 AQAGFITGQNLLMDGGAYPGTF 262 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 60/177 (33%), Positives = 92/177 (51%), Gaps = 15/177 (8%) Query: 86 DILVTNAGGPKPGDFREWTRDDWIAAVDANMLTPIELIKATVDGMIACGFGRIVNITSSA 145 D+LV NAG + G FR+ ++DDW A +D N+ + + K ++GM GFGRI+NI+S Sbjct: 83 DVLVNNAGITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEGMCERGFGRIINISS-- 140 Query: 146 VRAPIDILGLSN--GARSGLTGFVAGLARKTVAHNVTINNLLPGPFETDRLRSTATGAAK 203 V G +N A++G+ GF LA++ VT+N + PG TD +R+ Sbjct: 141 VNGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGTDMVRAIRP---- 196 Query: 204 ESGRTVDEVLADRRKLNPAGRFGDPAEFGDACAYLCSAQAGFITGQNLLMDGGAYPG 260 EVL P R G+P E A++ S++ G+ TG + ++GG Y G Sbjct: 197 -------EVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGLYMG 246 >gi|300313237|ref|YP_003777329.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 100 bits (249), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 83/269 (30%), Positives = 128/269 (47%), Gaps = 13/269 (4%) Query: 1 MDLGIKGKTALVCASSKGLGRACALSLAREGVHVTLLARGAEALEATAQAIRTETAATGA 60 M+L ++GK A+V S G+GRA A +LAREGV V ++AR LE A A+ + +TG Sbjct: 1 MELKLQGKAAVVTGGSLGIGRAVAEALAREGVRVAIVARSKGKLEEVASAL---SQSTGT 57 Query: 61 IITTVACDITTPEGRALALAACPAP----DILVTNA---GGPKPGDFREWTRDDWIAAVD 113 + VA D++ E A+A DILV A GG + + + + + ++ Sbjct: 58 EVIAVAADVSNTEQVEAAVAQAAQHFGRIDILVNGAAHPGGLVRSEIQHASPEGLLEDIN 117 Query: 114 ANMLTPIELIKATVDGMIACGFGRIVNITSSAVRAPIDILGLSNGARSGLTGFVAGLARK 173 ++ + KA M A G+GRI+NI R + G+ N A LT L+ + Sbjct: 118 IKVVGYMRFAKAVAPHMRAGGYGRIINIGGLTGRGSKQLSGMRNVAICHLT---KTLSDQ 174 Query: 174 TVAHNVTINNLLPGPFETDRLRSTATGAAKESGRTVDEVLADRRKLNPAGRFGDPAEFGD 233 +T+N + PG ET + AK G T ++V + K P R E D Sbjct: 175 LGPDGITVNVIHPGVVETPHIHELYEKEAKLQGLTPEQVEQNYAKATPIRRVLQVEEIAD 234 Query: 234 ACAYLCSAQAGFITGQNLLMDGGAYPGTF 262 +L S +A ITG+++ +DGG G + Sbjct: 235 VVLFLASPRAAAITGESIAVDGGITRGIY 263 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 94.0 bits (232), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 84/265 (31%), Positives = 123/265 (46%), Gaps = 28/265 (10%) Query: 5 IKGKTALVCASSKGLGRACALSLAREGVHVTLLARGAEALEATAQAIRTETAATGAIITT 64 ++G+ ALV +S+G+GRA A LAR+G V G E+ A AI AA G Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVI----GTATSESGAAAITEYLAALGPEAGR 61 Query: 65 VACDITTPEGRALALAACPAPD-----ILVTNAGGPKPGDFREWTRDDWIAAVDANMLTP 119 A ++AL D ILV NAG + ++W + + N+ Sbjct: 62 GAVLNVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAV 121 Query: 120 IELIKATVDGMIACGFGRIVNITSSAVRA--PIDILGLSN--GARSGLTGFVAGLARKTV 175 L +A + GM+ GRI+NITS A P G N A++G+ G LAR+ Sbjct: 122 GRLSRAVLRGMMKARTGRIINITSVVGEAGNP----GQMNYAAAKAGVAGMSRALAREIG 177 Query: 176 AHNVTINNLLPGPFETDRLRSTATGAAKESGRTVDEVLADRRKLNPAGRFGDPAEFGDAC 235 + N+T+N + PG +TD R+ ++S + ++ P GR G E A Sbjct: 178 SRNITVNCVAPGFIDTDMTRALNE---QQSAAILQQI--------PLGRLGAAEEIAYAV 226 Query: 236 AYLCSAQAGFITGQNLLMDGGAYPG 260 A+L S QA +ITG L ++GG Y G Sbjct: 227 AFLASPQAAYITGTTLNVNGGMYMG 251 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 89.0 bits (219), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 85/266 (31%), Positives = 117/266 (43%), Gaps = 39/266 (14%) Query: 5 IKGKTALVCASSKGLGRACALSLAREGVHVTLLARGAEALEATAQAIRTETAATGAIITT 64 ++GK AL+ +S G+GRA AL AR G + L AR +ALE A I A G + Sbjct: 4 LQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALI----CAQGGRASV 59 Query: 65 VACDI----TTPEGRALALAACPAPDILVTNAGGP---KPGDFREWTRDDWIAAVDANML 117 VA D+ T G A+AL DI + NAG KP E + W + AN+ Sbjct: 60 VAGDVGEAATHERGVAMALDEFGGLDIAINNAGAVGACKP--LAEISPQQWQDTLHANLT 117 Query: 118 TPIELIKATVDGMIACGFGRIVNITSSAVRAPIDILGLS--NGARSGLTGFVAGLARKTV 175 + + + M+A G G IV TSS V + + G++ A++GL G V G+A Sbjct: 118 SAFLGARCQIPAMLARGAGAIV-FTSSFVGSSAGLPGMAAYGAAKAGLMGLVKGIAADYA 176 Query: 176 AHNVTINNLLPGPFETD-----RLRSTATGAAKESGRTVDEVLADRRKLNPAGRFGDPAE 230 + N LLPG +TD + + A G L+ R P E Sbjct: 177 VQGIRANALLPGGVDTDMGGDPQQKQWAAG------------------LHAMKRIAQPEE 218 Query: 231 FGDACAYLCSAQAGFITGQNLLMDGG 256 A +L S A F+TG L DGG Sbjct: 219 IAAAALFLASPMASFVTGTALYADGG 244 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 849,578 Number of extensions: 35565 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 212 Number of HSP's successfully gapped: 31 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)