BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0020.p (388 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbasp... 714 0.0 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 181 1e-46 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 180 1e-46 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 158 5e-40 gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbasp... 105 5e-24 >gi|300312109|ref|YP_003776201.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 388 Score = 714 bits (1842), Expect = 0.0, Method: Compositional matrix adjust. Identities = 354/388 (91%), Positives = 375/388 (96%) Query: 1 MSYQAIPDTYTDLRDAVRDLCQTFPAEYWRKIDEARGYPEQFVDALTKAGWMAALIPQEY 60 MSY D++ DLRDAVRDLC+TFP EYWRKIDEARGYPEQFVDALTKAGW+AALIPQEY Sbjct: 1 MSYPTASDSHADLRDAVRDLCKTFPPEYWRKIDEARGYPEQFVDALTKAGWLAALIPQEY 60 Query: 61 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSERQKQQYLPKIASGELRL 120 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGS QK++YLP+IASGELRL Sbjct: 61 GGSGLGLTEASVIMEEINRTGGNSGACHGQMYNMGTLLRHGSPEQKERYLPRIASGELRL 120 Query: 121 QSMGVTEPTTGTDTTRVKTVAVKKGDRYVVNGQKVWISRVQHSDLMILLARTTPLDQVKK 180 QSM VTEPTTGTDTTR+KTVAVKKGDRYV+NGQKVWISRVQHSDLMILLARTTPL+QV + Sbjct: 121 QSMAVTEPTTGTDTTRIKTVAVKKGDRYVINGQKVWISRVQHSDLMILLARTTPLEQVSR 180 Query: 181 KSEGMSIFLVDLREAIGKGLEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL 240 KSEGMSIFLVDL+EAIG G+EVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL Sbjct: 181 KSEGMSIFLVDLKEAIGHGMEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYIL 240 Query: 241 DGLNAERTLIAAECIGDGYWFIDKASAYAKERVVFDRPIGMNQGVQFPIADAYIEIEAAN 300 DGLNAERTLIAAECIGDGYWFIDKA+ YA+ERVVFDRPIGMNQGVQFP+A+AYIE+EAAN Sbjct: 241 DGLNAERTLIAAECIGDGYWFIDKATQYARERVVFDRPIGMNQGVQFPLAEAYIEVEAAN 300 Query: 301 LMRYQACALFDAHQNCGAQANMAKYLAAKASWEAANVCLQTHGGFGFACEYDVERKFRET 360 LMRYQACALFDA Q+CGAQANMAKYLAAKA+WEAANVCLQTHGGFGFACEYDVERKFRET Sbjct: 301 LMRYQACALFDAQQSCGAQANMAKYLAAKAAWEAANVCLQTHGGFGFACEYDVERKFRET 360 Query: 361 RLYQVAPISTNLIFSYVAEHILGLPRSF 388 RLYQVAPISTNLIFSYVAEH+LGLPRSF Sbjct: 361 RLYQVAPISTNLIFSYVAEHVLGLPRSF 388 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 181 bits (458), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 120/372 (32%), Positives = 194/372 (52%), Gaps = 21/372 (5%) Query: 13 LRDAVRDLCQTFPAEYWRKIDEARGYPEQFVDALTKAGWMAALIPQEYGGSGLGLTEASV 72 LR+AV + A +ID + +P L G + + +EYGG+GLG + Sbjct: 18 LREAVAAFAHSEIAPRAAEIDRSDQFPMDLWKKLGDLGVLGITVSEEYGGAGLGYLAHII 77 Query: 73 IMEEINRTGGNSGACHGQMYNM--GTLLRHGSERQKQQYLPKIASGELRLQSMGVTEPTT 130 MEEI+R + G +G N+ + R+G+E QK++YLP++ SG+ + ++ ++EP Sbjct: 78 AMEEISRASASVGLSYGAHSNLCVNQIKRNGNEEQKRKYLPRLISGDF-IGALAMSEPNA 136 Query: 131 GTDTTRVKTVAVKKGDRYVVNGQKVWISRVQHSDLMILLARTTPLDQVKKKSEGMSIFLV 190 G+D +K A KKGDRYV+NG K+WI+ +D++++ A+T ++ + GM+ FLV Sbjct: 137 GSDVVSMKLRADKKGDRYVLNGSKMWITNGPDADVLVVYAKT----DLEAGARGMTAFLV 192 Query: 191 DLREAIGKGLEVRPILN---MVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAER 247 E KG V L+ M T EL F + E+P EN++G GRG ++ GL+ ER Sbjct: 193 ---EKGYKGFSVAQKLDKLGMRGSHTGELVFQDCEVPEENVLGGVGRGVNVLMSGLDFER 249 Query: 248 TLIAAECIGDGYWFIDKASAYAKERVVFDRPIGMNQGVQFPIADAYIEIEAANLMRY--- 304 ++++ +G +D Y +R F + IG Q +Q +AD Y + A Y Sbjct: 250 SVLSGGPLGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVG 309 Query: 305 QACALFDAHQNCGA----QANMAKYLAAKASWEAANVCLQTHGGFGFACEYDVERKFRET 360 QAC D+ A A Y A KA+W A +Q+ GG G+ EY V R +R+ Sbjct: 310 QACDRADSADKVRALRKDAAGAILYSAEKATWMAGE-AIQSLGGNGYINEYPVGRLWRDA 368 Query: 361 RLYQVAPISTNL 372 +LY++ ++ + Sbjct: 369 KLYEIGAGTSEI 380 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 119/367 (32%), Positives = 194/367 (52%), Gaps = 31/367 (8%) Query: 13 LRDAVRDLCQTFPAEYWRKIDEARGYPEQFVDALTKAGWMAALIPQEYGGSGLGLTEASV 72 +RDA+R Q + D +P + + L + G M +P+++GG+G+ + Sbjct: 10 IRDAMRHFAQERLLPFAADWDRNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVL 69 Query: 73 IMEEINRTGG---------NSGACHGQMYNMGTLLRHGSERQKQQYLPKIASGELRLQSM 123 +EEI G NS AC G ++GS QK+Q+L +A GE+ L Sbjct: 70 ALEEIAAGDGATSTIVSVQNSLAC-------GITEKYGSPTQKEQWLKPLARGEM-LGCF 121 Query: 124 GVTEPTTGTDTTRVKTVAVKKGDRYVVNGQKVWISRVQHSDLMILLARTTPLDQVKKKSE 183 +TEP TG+D ++T A + GD +++NG K +I+ +H+ + I+ A T D+ K + Sbjct: 122 CLTEPHTGSDAAAIRTRAERDGDDFILNGTKQFITSGKHAGVAIVFAVT---DRSAGK-K 177 Query: 184 GMSIFLVDLRE---AIGKGLEVRPILNMVNH--ETNELFFDNLEIPAENLIGEEGRGFRY 238 G+S FLV +G+ E M H +T ++ DN +PA L+G+EG G+R Sbjct: 178 GISCFLVPCDTPGFVVGRSEE-----KMGQHASDTVQIMLDNCRVPATALLGKEGEGYRI 232 Query: 239 ILDGLNAERTLIAAECIGDGYWFIDKASAYAKERVVFDRPIGMNQGVQFPIADAYIEIEA 298 L L A R IAA+ +G + A +YA++R F PI +Q V F +AD ++A Sbjct: 233 ALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMNTLLDA 292 Query: 299 ANLMRYQACALFDAHQNCGAQANMAKYLAAKASWEAANVCLQTHGGFGFACEYDVERKFR 358 A LM ++A L D + C +A+MAK A++A+ + A+ +Q HGG G+ ++ VER +R Sbjct: 293 ARLMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGYTSDFPVERIYR 352 Query: 359 ETRLYQV 365 + R+ Q+ Sbjct: 353 DVRICQI 359 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 158 bits (400), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 112/373 (30%), Positives = 186/373 (49%), Gaps = 10/373 (2%) Query: 13 LRDAVRDLCQTFPAEYWRKIDEARGYPEQFVDALTKAGWMAALIPQEYGGSGLGLTEASV 72 + + RD A + K DE +P + + G+ P+ GG GL +A+V Sbjct: 12 FQQSARDFAAGEMAPFAAKWDEEAHFPLDVIAKAGELGFCGLYTPEADGGLGLSRLDATV 71 Query: 73 IMEEINRTGGNSGACHGQMYNMGTLL--RHGSERQKQQYLPKIASGELRLQSMGVTEPTT 130 + EE+ R S A + ++NM + + G K Q+ ++A+G ++ S +TEP + Sbjct: 72 VFEELARAC-TSTAAYLTIHNMVSWMIASWGQPSLKAQWCEQLAAGR-KIGSYCLTEPGS 129 Query: 131 GTDTTRVKTVAVKKGDRYVVNGQKVWISRVQHSDLMILLARTTPLDQVKKKSEGMSIFLV 190 G+D +KT A + YV+NG K +IS +D+++++ART + + G+S +V Sbjct: 130 GSDAASLKTTARLEDGHYVLNGSKAFISGAGSTDVLVVMARTG-----GEGARGVSAIVV 184 Query: 191 DLREAIGKGLEVRPILNMVNHETNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTLI 250 + + + T + FDN+++PA++L+G+EG+GF + + GL+ R I Sbjct: 185 PGNAPGVSYGKKESKMGWNSQPTRTISFDNVKVPADHLLGQEGQGFIFAMKGLDGGRINI 244 Query: 251 AAECIGDGYWFIDKASAYAKERVVFDRPIGMNQGVQFPIADAYIEIEAANLMRYQACALF 310 A +G +D A AY KER F RPI Q +QF +AD E+ AA M A Sbjct: 245 ATCSVGAAQAALDAAHAYMKERKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATKL 304 Query: 311 DA-HQNCGAQANMAKYLAAKASWEAANVCLQTHGGFGFACEYDVERKFRETRLYQVAPIS 369 DA MAK LA + N LQ HGG+G+ EY +ER FR+ R++Q+ + Sbjct: 305 DAGSPEATTYCAMAKRLATDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGT 364 Query: 370 TNLIFSYVAEHIL 382 ++ ++ +L Sbjct: 365 NEIMRVIISRKLL 377 >gi|300310270|ref|YP_003774362.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 415 Score = 105 bits (263), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 78/297 (26%), Positives = 140/297 (47%), Gaps = 22/297 (7%) Query: 31 KIDEARGYPEQFVDALTKAGWMAALIPQEYGGSGLGLTEASVIMEEINRTGGN-SGACHG 89 K DE F A+ AG++ AL+P+ +GG+ L + ++ EE+ N A G Sbjct: 38 KPDERFYATRPFYQAMADAGFIKALLPESFGGTALSTLDFALAAEELTAVDVNVPSALLG 97 Query: 90 QMYNMGTLLRHGSERQKQQYLPKIASGELRLQSMGVTEPTTGTD------TTRVKTVAVK 143 + ++ HG+ QKQ++LP RL ++ TE T G + + V+T A Sbjct: 98 TGLGLHAVVHHGTPEQKQRFLPDFIEDGSRLAALAFTEVTGGANYDSTDPSVGVQTFARL 157 Query: 144 KGDRYVVNGQKVWISRVQHSD-----LMILLARTTPLDQVKKKSEGMSIFLVDLREAIGK 198 +GD +V+NG K + + D L ++ RT P + +++ +V Sbjct: 158 EGDEWVINGAKHYTTNGTGWDGKGAHLYSVICRTDP---ALPPQQSLAVIMV---PGNAP 211 Query: 199 GLEVRPILNMVNHE---TNELFFDNLEIPAENLIGEEGRGFRYILDGLNAERTLIAAECI 255 G+E+ +L+ + H + + F+N+ +PA N++G+ G G + + LI + C+ Sbjct: 212 GIEIVGLLDTMGHRATISPRVHFNNVRVPASNIVGKPGDGISMLEAAFSWTAALIGSACV 271 Query: 256 GDGYWFIDKASAYAK-ERVVFDRPIGMNQGVQFPIADAYIEIEAANLMRYQACALFD 311 G D A +A+ +R P+ +Q V F +AD + IEAA + +++C D Sbjct: 272 GVMRTAFDIALRFAQSDRRSGTIPVIEHQNVGFMLADIKMRIEAARYLTWKSCHQLD 328 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,186,866 Number of extensions: 48637 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 151 Number of HSP's successfully gapped: 9 Length of query: 388 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 301 Effective length of database: 1,207,925 Effective search space: 363585425 Effective search space used: 363585425 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)