BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0017.p (546 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 647 0.0 gi|300309505|ref|YP_003773597.1| 2,3-bisphosphoglycerate-depende... 435 e-123 gi|300312025|ref|YP_003776117.1| bifunctional glutathione-depend... 190 3e-49 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 131 1e-31 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 91 2e-19 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 647 bits (1670), Expect = 0.0, Method: Compositional matrix adjust. Identities = 320/340 (94%), Positives = 332/340 (97%) Query: 1 MKIQAAVLREMTEAYPFAVSQPLKIEELDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRP 60 MKIQAAVLREM++A+PFAVSQPLKIEELDLDPPGPGEVLV+MKAAGLCHSDLSVITGVRP Sbjct: 1 MKIQAAVLREMSDAHPFAVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDLSVITGVRP 60 Query: 61 RPVPMALGHEASAEVVEVGSGVSDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 RPVPMALGHEASAEVVEVGSGV+DLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN Sbjct: 61 RPVPMALGHEASAEVVEVGSGVNDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATN 120 Query: 121 AVGELLSGQRRLHDRAQHVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT 180 VGELLSGQRRL D+ Q VHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT Sbjct: 121 TVGELLSGQRRLRDKTQPVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLT 180 Query: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCTMLGALASGAREVIAVDLHDSKLEVARALGAT 240 GVGAAVNTAEVKAGTTAAVLGLGGVGLC MLGALASGAREVIAVDLHDSKL +AR+LGAT Sbjct: 181 GVGAAVNTAEVKAGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGAT 240 Query: 241 ATVNARDPDAVEKVKALTKGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPNQNW 300 ATVNARDPDAVEKV+ALT+GGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAP Q+W Sbjct: 241 ATVNARDPDAVEKVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHW 300 Query: 301 PLQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKL 340 LQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGL+QAGKL Sbjct: 301 ALQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLFQAGKL 340 >gi|300309505|ref|YP_003773597.1| 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase [Herbaspirillum seropedicae SmR1] Length = 248 Score = 435 bits (1118), Expect = e-123, Method: Compositional matrix adjust. Identities = 208/211 (98%), Positives = 210/211 (99%) Query: 336 QAGKLLKEAGFSFDLAYTSVLKRAIRTLWTTLDEMDQMYIPIKNDWRLNERHYGALQGLN 395 QAGKLLKEAGF+FDLAYTSVLKRAIRTLWTTLDEMDQMYIPIKNDWRLNERHYGALQGLN Sbjct: 38 QAGKLLKEAGFTFDLAYTSVLKRAIRTLWTTLDEMDQMYIPIKNDWRLNERHYGALQGLN 97 Query: 396 KAETAAQYGDEQVLVWRRSYDTPPNPLTPGEERDAFGDARYAGLSREQVPLTECLKDTVA 455 KAETAAQYGDEQVLVWRRSYDTPPNPLTPGEERDAFGD RYAGLSREQVPLTECLKDTVA Sbjct: 98 KAETAAQYGDEQVLVWRRSYDTPPNPLTPGEERDAFGDPRYAGLSREQVPLTECLKDTVA 157 Query: 456 RVLPAWNDSIAPAIRAGKQIIISAHGNSLRALIKYLDGISDNDIVGLNIPNGQPLVYELD 515 RVLPAWND+IAPAIRAGKQIIISAHGNSLRALIKYLDGISDNDIVGLNIPNGQPLVYELD Sbjct: 158 RVLPAWNDAIAPAIRAGKQIIISAHGNSLRALIKYLDGISDNDIVGLNIPNGQPLVYELD 217 Query: 516 ADLKPIKSYYLGDQSAIEAALKAVANQGKSK 546 ADLKPIKSYYLGDQSAIEAALKAVANQGKSK Sbjct: 218 ADLKPIKSYYLGDQSAIEAALKAVANQGKSK 248 >gi|300312025|ref|YP_003776117.1| bifunctional glutathione-dependent formaldehyde dehydrogenase/alcohol dehydrogenase class III oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 368 Score = 190 bits (482), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 120/343 (34%), Positives = 180/343 (52%), Gaps = 12/343 (3%) Query: 1 MKIQAAVLREMTEAYPFAVSQPLKIEELDLDPPGPGEVLVKMKAAGLCHSDLSVITGVRP 60 MK +AAV + +PL IEE++L P GEVLV++KA G+CH+D ++G P Sbjct: 1 MKTRAAV--------AWQAGKPLTIEEVELGGPKAGEVLVEIKATGICHTDYYTLSGADP 52 Query: 61 RPV-PMALGHEASAEVVEVGSGVSDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAAT 119 + P LGHE + VV+VG GV L+ D V+ ++ P C C C+ + LC+ AT Sbjct: 53 EGIFPAILGHEGAGVVVDVGPGVKSLKKDDHVIPLYTPECRECKFCLSQKTNLCQAIRAT 112 Query: 120 NAVGELLSGQRRLHDRAQHVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVL 179 G + R + + H++G S F+++ VV + KI +D + GC V Sbjct: 113 QGRGLMPDATSRFSLDGKPLFHYMGTSTFSNYIVVPEIALAKIRSDAPFDKVCYIGCGVT 172 Query: 180 TGVGAAVNTAEVKAGTTAAVLGLGGVGLCTMLGALASGAREVIAVDLHDSKLEVARALGA 239 TGVGA + TA+V+AG V GLGG+GL + A GA ++I VDL+ ++ +AR G Sbjct: 173 TGVGAVLFTAKVEAGANVVVFGLGGIGLNVIQAAKMVGANKIIGVDLNPAREAMARKFGM 232 Query: 240 TATVNARD-PDAVEKVKALTKGGVDYAFEMAGSVQAMEAAYRMTRRG-GMTVTAGLPAPN 297 T +N +D P+ V+ + +T GG DY+FE G+ M + +G G ++ G+ A Sbjct: 233 TDFINPKDVPNVVDAIIGMTDGGADYSFECIGNTTTMRQSLECCHKGWGQSIIIGVAAAG 292 Query: 298 QNWPLQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKL 340 Q + LV R KGS G D+P+ + Y GKL Sbjct: 293 QEISTRPFQLVT-GRVWKGSAFGGARGRTDVPKIVDWYMEGKL 334 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 131 bits (330), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 110/351 (31%), Positives = 176/351 (50%), Gaps = 44/351 (12%) Query: 3 IQAAVLREMTEAYPFAVSQPLKIEELDLDPPGPGEVLVKMKAAGLCHSDLSVITG---VR 59 ++AAV+RE +PL IE++ + P PG++LVK +A+G+CH+DL G V+ Sbjct: 5 MKAAVVREF--------GKPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVK 56 Query: 60 PRPVPMALGHEASAEVVEVGSGVSDLRAGDLVVLVFV-PSCGHCLPCMEGRPALCEPGAA 118 P P P GHE + V VG+GV ++ GD V + ++ +CG C PC G LC A Sbjct: 57 PTP-PFIPGHEGTGYVAAVGAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLC---AE 112 Query: 119 TNAVGELLSGQRRLHDRAQHVHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAV 178 G ++G +FA++ + + + ++D AA CA Sbjct: 113 QQNTGYSVNG------------------SFAEYGLADPKFVGHLPDNLDFGPAAPVLCAG 154 Query: 179 LTGVGAAVNTAEVKAGTTAAVLGLGGVGLCTMLGALASGAREVIAVDLHDSKLEVARALG 238 +T V + EV+ G + G+GG+G + A A G V+A D+H+ KL +A+ LG Sbjct: 155 VT-VYKGLKETEVRPGEWVVISGIGGLGHMAVQYAKAMG-MHVVAADIHEDKLALAKKLG 212 Query: 239 ATATVNARDPDAVEKVKALTKGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPNQ 298 A TV+ R+ +AV +V+ + GG A A S +AME A+ R G GLP + Sbjct: 213 ADLTVDGRNHNAVAEVQRII-GGAHGALVTAVSPKAMEQAFGFLRARGTMALVGLPPGDI 271 Query: 299 NWPLQQVSLVAEERTVKGSYIGSCVPVRDIPRYIGLYQAGKLLKEAGFSFD 349 + P+ + V + TV+GS +G+ +D+ + GK+ A F++D Sbjct: 272 SLPV--FNTVLKRITVRGSIVGT---RQDLEESLVFAAEGKV--AAHFTWD 315 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 91.3 bits (225), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 86/300 (28%), Positives = 129/300 (43%), Gaps = 25/300 (8%) Query: 23 LKIEELDLDPPGPGEVLVKMKAAGLCHSDLSVI----TGVRPRPVPMALGHEASAEVVEV 78 L+I+ LD P ++ VK+KA G+C SDL GV PM LGHE S ++ EV Sbjct: 13 LRIQALDTGALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEV 72 Query: 79 GSGVSDLRAGDLVVLVFVPSCGHCLPCMEGRPALCEPGAATNAVGELLSGQRRLHDRAQH 138 G+ V+D+ G + + CG C C EG+ C R + A Sbjct: 73 GTAVTDIAPGTRIAISPSRPCGACKYCQEGKQNHCL--------------DMRFYGSAMR 118 Query: 139 VHHHLGVSAFADHAVVSRRSCVKIEADIDPVQAALFGCAVLTGVGAAVNTAEVKAGTTAA 198 H G AF ++ R + + +AA+ L+ AV A G Sbjct: 119 TPHVQG--AFRQEIIIERSQAHVVADHVSDGEAAM--AEPLSVALHAVRRAGPLVGKRVL 174 Query: 199 VLGLGGVGLCTMLGALASGAREVIAVDLHDSKLEVARALGATATVNARDPDAVEKVKALT 258 V G G +G ++ A +GA EV+ D+ L+ A +GA + +N + A K A Sbjct: 175 VTGCGPIGALVVVAARRAGALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAAD 234 Query: 259 KGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPNQNWPLQQVSLVAEERTVKGSY 318 KG D FE +G+ +A+ A R + V GL Q +P LV +E +G++ Sbjct: 235 KGTFDVLFEASGNERALVGALEALRPQAIIVQVGLGGDIQ-FPAN--LLVGKELEWRGAF 291 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,670,606 Number of extensions: 70805 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 267 Number of HSP's successfully gapped: 9 Length of query: 546 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 456 Effective length of database: 1,193,720 Effective search space: 544336320 Effective search space used: 544336320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)