BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0013.0016.p (463 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase... 795 0.0 gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillu... 74 3e-14 gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Her... 71 2e-13 gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptid... 59 1e-09 >gi|300309506|ref|YP_003773598.1| membrane-bound metallopeptidase [Herbaspirillum seropedicae SmR1] Length = 470 Score = 795 bits (2052), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/449 (94%), Positives = 439/449 (97%) Query: 15 VQAQPITERTRQKQAAEKEQADLQQRLTTLKRDIDQTETAKDNAADALAASEQAISKANR 74 VQ+QPITER RQKQAAEKE+ADLQQRL LKRDID TETAKDNAADALAASEQAISKANR Sbjct: 22 VQSQPITERARQKQAAEKERADLQQRLANLKRDIDHTETAKDNAADALAASEQAISKANR 81 Query: 75 SLRDLAQEQQATEARLAQLVKQLNELTALVNAQQAQLSKLLREQYVAGNEDRIKLLLSGD 134 SLRDLAQEQQ TEARL QL++QLNELTALVNAQQAQLSKLLREQYVAGNEDRIKLLLSGD Sbjct: 82 SLRDLAQEQQQTEARLNQLIRQLNELTALVNAQQAQLSKLLREQYVAGNEDRIKLLLSGD 141 Query: 135 NPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKADTQNAKFELDEIAQEEQEQKKV 194 NPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKADTQNAKFEL+EIAQEE EQKKV Sbjct: 142 NPNRINRELQYMGYVSQAQAKLIETLRANQQAIRANKADTQNAKFELEEIAQEELEQKKV 201 Query: 195 LEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSLVDKLAQIIEQQKKAEAEAREK 254 LEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSLVDKLAQIIEQQKKAEAEAREK Sbjct: 202 LEREKGRRQTLLSQLSTKLNAQRQEAGRIEQDQQRLSSLVDKLAQIIEQQKKAEAEAREK 261 Query: 255 RRQEQLAKAKAEREKRLAEQRARAEKRAAEAARAAKEGKPAPKMPDPADAIDDDEPPQQA 314 RRQEQLAKAKAEREKRLAEQRARAEKRAAEAA+AAKEGKPAPKMPDPADAIDDDEPPQQA Sbjct: 262 RRQEQLAKAKAEREKRLAEQRARAEKRAAEAAKAAKEGKPAPKMPDPADAIDDDEPPQQA 321 Query: 315 SLGRNDTLPEPQADNFGKPFASLRGQLHLPVRGELVSKFGSRRGDGPTSRGLFLRAQEGA 374 SLGRNDT+PEPQ DNFGKPFASLRGQLHLPV+G+L++KFGSRRGDGPTSRGLF+RA EGA Sbjct: 322 SLGRNDTVPEPQPDNFGKPFASLRGQLHLPVKGDLMAKFGSRRGDGPTSRGLFIRAHEGA 381 Query: 375 EVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVKTGETIATAGN 434 EVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVK G+TIATAGN Sbjct: 382 EVRAVAAGRVVFADWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVKAGDTIATAGN 441 Query: 435 SGGNEQSGLYFEMRYQGRAFDPQGWVTSR 463 SGGNEQSGLYFEMRYQGRAFDPQGWVTSR Sbjct: 442 SGGNEQSGLYFEMRYQGRAFDPQGWVTSR 470 >gi|300313095|ref|YP_003777187.1| NlpD lipoprotein [Herbaspirillum seropedicae SmR1] Length = 254 Score = 73.9 bits (180), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 36/93 (38%), Positives = 59/93 (63%), Gaps = 2/93 (2%) Query: 364 RGLFLRAQEGAEVRAVAAGRVVFA-DWLRGFGNLIIVDHGNQYMTIYGNNQSVLKRAGDL 422 +G+ + EG V A AAG+V +A +RG+GN++I+ H Q +++Y +N+++L + G Sbjct: 155 KGIDIIGSEGQSVVAAAAGKVTYAGHGIRGYGNMVIIKHTPQLLSVYAHNKTILVKEGQT 214 Query: 423 VKTGETIATAGNSGGNEQSGLYFEMRYQGRAFD 455 V G+ IAT GNS N+ LYFE+R G+ + Sbjct: 215 VTRGQQIATMGNSDTNKVK-LYFEIRRNGKPIN 246 >gi|300312264|ref|YP_003776356.1| NlpD precursor lipoprotein [Herbaspirillum seropedicae SmR1] Length = 332 Score = 70.9 bits (172), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 39/122 (31%), Positives = 68/122 (55%), Gaps = 8/122 (6%) Query: 343 LPVRGELVSKFGSRRGDGPTSRGLFLRAQEGAEVRAVAAGRVVFA-DWLRGFGNLIIVDH 401 P G++V F + +G+ + + G V A AG+V++A +RG+GNL+I+ H Sbjct: 218 WPAEGKVVGTFEGGK------KGVDIAGKAGQAVLAAGAGKVMYAGSGIRGYGNLVIIKH 271 Query: 402 GNQYMTIYGNNQSVLKRAGDLVKTGETIATAGNSGGNEQSGLYFEMRYQGRAFDPQGWVT 461 + ++ Y +NQ++L + G V G+ IA G S ++ L+FE+R QG+ DP ++ Sbjct: 272 TSNLLSAYAHNQTILVKEGQSVTKGQKIAEMGKS-DSDTVKLHFEIRQQGKPVDPSKYLP 330 Query: 462 SR 463 R Sbjct: 331 PR 332 >gi|300309687|ref|YP_003773779.1| transmembrane metalloendopeptidase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 58.5 bits (140), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 25/68 (36%), Positives = 45/68 (66%) Query: 393 FGNLIIVDHGNQYMTIYGNNQSVLKRAGDLVKTGETIATAGNSGGNEQSGLYFEMRYQGR 452 FGN++ +DHGN +T Y + + + GD+VK G+ +A G++G + + L+FE+R +G Sbjct: 238 FGNMLEIDHGNNIITRYAHASRLFVKVGDIVKRGQHVADVGSTGRSTGAHLHFEVRIRGI 297 Query: 453 AFDPQGWV 460 A DP+ ++ Sbjct: 298 AQDPRKFL 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.127 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 979,742 Number of extensions: 33229 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 4 Length of query: 463 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 374 Effective length of database: 1,198,455 Effective search space: 448222170 Effective search space used: 448222170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)