BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0179.p
         (259 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro...    71   9e-14
gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr...    55   4e-09
gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil...    55   6e-09

>gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone
           hydrolase/4-carboxymuconolactone decarboxylase
           [Herbaspirillum seropedicae SmR1]
          Length = 316

 Score = 70.9 bits (172), Expect = 9e-14,   Method: Compositional matrix adjust.
 Identities = 61/227 (26%), Positives = 103/227 (45%), Gaps = 4/227 (1%)

Query: 20  VLCIHGLGGSSNTWTPILPALAGR-KVIRLDLPGSGRSKLPAQALTIEAYVLAVRQVLKA 78
           V+  H LG     W  +   LA   +VI  D  G G S+      ++        ++L+ 
Sbjct: 77  VVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLADDAARLLRE 136

Query: 79  LKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVAPPEPARPAIRARAELARQGA 138
           L    V  +  SMG +V Q  A+ +P  V +LVL     A P+ AR A + R    R  A
Sbjct: 137 LDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQRIATVR--A 194

Query: 139 EKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCEALASAQSAP-IEE 197
           E ++ IADA++     +  +++    +   R+ ++     GY   C A+ +  +A  +  
Sbjct: 195 EGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVDTAARLGS 254

Query: 198 IAVQTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFEK 244
           I V TL++ G+ D   P A   A+   I G+++ V+ G  H +  E+
Sbjct: 255 IRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQ 301


>gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone
           hydrolase [Herbaspirillum seropedicae SmR1]
          Length = 261

 Score = 55.5 bits (132), Expect = 4e-09,   Method: Compositional matrix adjust.
 Identities = 63/232 (27%), Positives = 103/232 (44%), Gaps = 12/232 (5%)

Query: 9   IAVEVEGDGA-PVLCI-HGLGGSSNTWTPILPA-LAGRKVIRLDLPGSGRSKLPAQALTI 65
           +  ++EGD + PVL + + LG S + W P +P  L   +V+R D  G G+S++     +I
Sbjct: 11  LHYQLEGDASLPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQSEVTPGECSI 70

Query: 66  EAYVLAVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLF--GPLVAPPEPA 123
                 V  +L  L I        SMG       AV +P  +  L+L   G  +   E  
Sbjct: 71  AQLGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNTGAKIGTAE-- 128

Query: 124 RPAIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQS 183
             A  +R E  R+  E +  IA A+V+   + E   ++P  +  +   ++  P EGYA +
Sbjct: 129 --AWNSRIETVRR--EGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEGYAAA 184

Query: 184 CEALASAQ-SAPIEEIAVQTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLE 234
           C A+        I  I   TL++ G  D   P A+   M   I G++ +  +
Sbjct: 185 CAAVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQYVEFD 236


>gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 289

 Score = 55.1 bits (131), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 43/122 (35%), Positives = 59/122 (48%), Gaps = 6/122 (4%)

Query: 17  GAPVLCIHGLGGSSNTWTPILPALA-GRKVIRLDLPGSGRSKLPAQA-LTIEAYVLAVRQ 74
           G P+L +HG+ G   T+  +   L  G + I LD  G G+S   A A    +AYV  V  
Sbjct: 25  GFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYTDAYVADVEA 84

Query: 75  VLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVL--FGPLVAPPEPARPAIRARAE 132
           ++  L +   D+L HSMG I A  +A  +P  V  L++   GP      P   A R R E
Sbjct: 85  LVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPG--ATRIRRE 142

Query: 133 LA 134
           LA
Sbjct: 143 LA 144


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.131    0.369 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 718,383
Number of extensions: 26928
Number of successful extensions: 155
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 157
Number of HSP's successfully gapped: 3
Length of query: 259
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 176
Effective length of database: 1,226,865
Effective search space: 215928240
Effective search space used: 215928240
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 129 (54.3 bits)