BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0172.p (254 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 61 1e-10 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 60 1e-10 gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydroge... 60 1e-10 gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein... 59 3e-10 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 59 3e-10 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 60.8 bits (146), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 63/242 (26%), Positives = 107/242 (44%), Gaps = 10/242 (4%) Query: 18 LNGLKIFIFGAGSGMGAAIAKMAAARGAKIALAGRNLEKLKSVAQQLGQAVIGTYSVDLA 77 LNG + G SG+G A A+ AA+GA++ + GR +L + +G A G + D + Sbjct: 5 LNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA-DAS 63 Query: 78 DQKRVEEVLAE----HGPYDHIVTTAAELTFTPFVDLSDEQISRMLSAKFWGPINIGRAA 133 ++ V A+ G D + A P +++E R+ G + + A Sbjct: 64 VLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTVQKA 123 Query: 134 SRHLNAQGSALFFSGLAAYRQAPGASLVGVLNIGLESLAAALAIELKPK--RFNVISPGV 191 L S + S + S+ + + A + A++LK + R NV+SPG Sbjct: 124 LPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVSPGP 183 Query: 192 VDADTWAAM-PEDARSAFFQQVSNTLPVGRVGSVTDAAHAA--LAVLENGFINGTVINVD 248 + + P+DAR F +++ +P+GR+G + A LA F+NG + VD Sbjct: 184 IRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIELFVD 243 Query: 249 GG 250 GG Sbjct: 244 GG 245 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 60.5 bits (145), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 62/246 (25%), Positives = 107/246 (43%), Gaps = 17/246 (6%) Query: 18 LNGLKIFIFGAGSGMGAAIAKMAAARGAKI-------ALAGRNLEKLKSVAQQLGQAVIG 70 L G + GA G+G AIA A +GAK+ + A E L ++ + G+ + Sbjct: 6 LQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESGAAAITEYLAALGPEAGRGAVL 65 Query: 71 TYSVDLADQKRVEEVLAEHGPYDHIVTTAAELTFTPFVDLSDEQISRMLSAKFWGPINIG 130 + A VE+V + G +V A + + DE+ +++ + Sbjct: 66 NVNDAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLS 125 Query: 131 RAASRHLNA--QGSALFFSGLAAYRQAPGASLVGVLNIGLESLAAALAIELKPKRF--NV 186 RA R + G + + + PG G+ ++ ALA E+ + N Sbjct: 126 RAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNC 185 Query: 187 ISPGVVDADTWAAMPEDARSAFFQQVSNTLPVGRVGSVTDAAHAA--LAVLENGFINGTV 244 ++PG +D D A+ E +A QQ+ P+GR+G+ + A+A LA + +I GT Sbjct: 186 VAPGFIDTDMTRALNEQQSAAILQQI----PLGRLGAAEEIAYAVAFLASPQAAYITGTT 241 Query: 245 INVDGG 250 +NV+GG Sbjct: 242 LNVNGG 247 >gi|300310400|ref|YP_003774492.1| 2-deoxy-D-gluconate 3-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 241 Score = 60.5 bits (145), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 66/239 (27%), Positives = 104/239 (43%), Gaps = 14/239 (5%) Query: 20 GLKIFIFGAGSGMGAAIAKMAAARGAKIALAGRNLEKLKSVAQQLGQAVIGTYSVDLADQ 79 G + I GAG G+G A A + A RGA++ R L + +++G VI +VDL+D Sbjct: 5 GKTVIITGAGRGIGRACALLLAQRGAQVIALARTASDLARLQEEIGARVI---AVDLSDP 61 Query: 80 KRVEEVLAEHGPYDHIVTTAAELTFTPFVDLSDEQISRMLSAKFWGPINIGRAASRHLNA 139 +AE G D ++ +A +++SDE +L + +A +R A Sbjct: 62 AAARRAMAEAGTADFLINSAGINVLESVLEMSDEGYEAVLGVNLRAALITCQAFARMRIA 121 Query: 140 Q---GSALFFSGLAAYRQAPGASLVGVLNIGLESLAAALAIELKPK--RFNVISPGVVDA 194 Q G+ + + +A +R GLE LA EL P R N ++P + Sbjct: 122 QGGGGAMVNITSIAGHRGFEQHLCYAASKAGLEGATRVLARELGPHGIRVNAVAPTITLT 181 Query: 195 D-TWAAMPEDARSAFFQQVSNTLPVGRVGSVTDAAHAALAVL--ENGFINGTVINVDGG 250 + AA + A+S P R S D A + +L + +NG V+ VDGG Sbjct: 182 ELAEAAWSDPAKSGPMMARH---PSARFASPEDVARSIAMLLSPDAAMLNGAVVPVDGG 237 >gi|300310391|ref|YP_003774483.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 283 Score = 59.3 bits (142), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 54/184 (29%), Positives = 88/184 (47%), Gaps = 6/184 (3%) Query: 18 LNGLKIFIFGAGSGMGAAIAKMAAARGAKIALAGRNLEKLKSVAQQLGQAVIGTYSVDLA 77 L G +I++ GA SG+GA +A+ A A GA++AL+ R E L+ VA +A I V Sbjct: 36 LRGRRIWLIGASSGIGAELARQALAAGARVALSARRAEVLEEVAGAHPEAFIAPLDVLCH 95 Query: 78 DQKRVE--EVLAEHGPYDHIVTTAAELTFTPFVDLSDEQISRMLSAKFWGPINIGRAASR 135 D R + ++ G D ++ AA ++ +++ + + AA Sbjct: 96 DAWRDQHARIVEAFGGVDLLIFCAARYRPERSWEVQEDEAEHTIRTNLASVYSALGAALP 155 Query: 136 HLNAQGSA--LFFSGLAAYRQAPGASLVGVLNIGLESLAAALAIELKPKRFNV--ISPGV 191 + A+GS + +A Y PGA++ G L +LA L +L+PK NV ++PG Sbjct: 156 DMLARGSGGIALVASVAGYVGLPGATVYGPGKAALINLAEILYSDLRPKGVNVYLVNPGF 215 Query: 192 VDAD 195 V D Sbjct: 216 VKTD 219 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 59.3 bits (142), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 64/244 (26%), Positives = 114/244 (46%), Gaps = 23/244 (9%) Query: 23 IFIFGAG-SGMGAAIAKMAAARGAKIALAGRNLEKLKSVAQQLGQAVIGTYSVDLADQKR 81 I GAG +G+G + A++ AA+GA+I + + A +LG +G + D+ D+ Sbjct: 9 IITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVA-DVTDKAA 67 Query: 82 VEE----VLAEHGPYDHIVTTAAELTFTPFVDLSDEQISRMLSAKFWGPINIGRAASRHL 137 E VL + G D +V A ++++ + R+L G + + +A + Sbjct: 68 CEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQAVLPAM 127 Query: 138 NAQ--GSALFFSGLAAYRQAPGASLVG-----VLNIGLESLAAALAIELKPK--RFNVIS 188 AQ GS + S ++A R G ++G G+ LA A+A E P+ R N I+ Sbjct: 128 QAQQSGSIVCISSVSAQR---GGGILGGPHYSAAKAGVLGLARAMAREFGPQGIRVNCIT 184 Query: 189 PGVVDADTWAAMPEDARSAFFQQVSNTLPVGRVGSVTDAAHAA--LAVLENGFINGTVIN 246 PG++ D + + A +++ T+P+GR+G D LA + + G ++ Sbjct: 185 PGLISTDINKDILSAEKKA---EIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTGVTLD 241 Query: 247 VDGG 250 V+GG Sbjct: 242 VNGG 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 683,908 Number of extensions: 23305 Number of successful extensions: 117 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 119 Number of HSP's successfully gapped: 14 Length of query: 254 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 171 Effective length of database: 1,226,865 Effective search space: 209793915 Effective search space used: 209793915 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)