BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0162.p
         (279 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate hydratase/2...   137   7e-34
gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578...    93   2e-20
gi|300310344|ref|YP_003774436.1| fumarylacetoacetate hydrolase f...    57   2e-09

>gi|300310596|ref|YP_003774688.1| 2-keto-4-pentenoate
           hydratase/2-oxohepta-3-ene-1,7-dioic acid hydratase
           [Herbaspirillum seropedicae SmR1]
          Length = 281

 Score =  137 bits (346), Expect = 7e-34,   Method: Compositional matrix adjust.
 Identities = 72/202 (35%), Positives = 111/202 (54%), Gaps = 2/202 (0%)

Query: 71  KILCVGINYLSHVKETGRELPKHPMIFARFADSQTGHLQPIIRPHASEKLDFEGELAVVI 130
           K +C+G+NY  H  E+   +P  P++F ++  +  G    +  P  S+K D+E EL V+I
Sbjct: 72  KFICIGLNYADHAAESNLPIPAEPVVFNKWTSAVVGPNDNVKIPRGSKKTDWEVELGVII 131

Query: 131 GRKARHIKAAEALDYVAGYACYNDGSVRDWQ-KHTIQFIPGKNFPQTGGFGPWLVTPDEA 189
           G+   +I   +A+ +VAGY   ND S R++Q +    +  GK     G  GPWLVT DE 
Sbjct: 132 GKGGSYIDEKDAMSHVAGYCVVNDVSEREYQIERGGTWDKGKGCDTFGPIGPWLVTRDEV 191

Query: 190 GDPSQLSLVTRLNGTVVQEARTDDLIFPIPALIEYCSTFTELNPGDVIITGTTGGVGAFR 249
            DP +L +   ++G   Q   T  +IF +  ++ Y S F  L PGDVI TGT  GVG   
Sbjct: 192 ADPQKLGMWLEVDGKRYQNGNTSTMIFNVAHIVSYLSRFMSLQPGDVISTGTPPGVGMGV 251

Query: 250 EP-PLWMKPGDVVEVEISGLGK 270
           +P  ++++ G  + + I GLG+
Sbjct: 252 KPEAVYLRAGQTIRLGIDGLGE 273


>gi|300312873|ref|YP_003776965.1| hypothetical protein Hsero_3578
           [Herbaspirillum seropedicae SmR1]
          Length = 227

 Score = 93.2 bits (230), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 67/204 (32%), Positives = 99/204 (48%), Gaps = 13/204 (6%)

Query: 70  EKILCVGINYLSHVKETGRELPKHP-MIFARFADSQTGHLQPIIRPHASEKLDFEGELAV 128
            +I CVG NY +H +E G +  + P   F + AD+       +  P A+     E EL V
Sbjct: 24  RRIYCVGRNYAAHAREMGHDPDREPPFFFMKPADAVVPTDSVVPYPVATSDYHHEIELVV 83

Query: 129 VIGRKARHIKAAEALDYVAGYACYNDGSVRDWQKHTIQ----FIPGKNFPQTGGFGPWLV 184
            + +  R+I   +AL+ V GYA   D + RD Q    +    +   K F Q+    P +V
Sbjct: 84  ALAKGGRNIPVEQALELVFGYAVGLDMTRRDIQSQAKKLGRPWDMAKGFDQSAPCAP-IV 142

Query: 185 TPDEAGDPSQLSLVTRLNGTVVQEARTDDLIFPIPALIEYCSTFTELNPGDVIITGTTGG 244
              +AG P Q ++  ++NG V Q+    DLI+ +   I Y S   EL PGD+I TGT  G
Sbjct: 143 PVAQAGHPDQGAVWLKVNGEVRQQGDLADLIWSVAETISYLSGLVELFPGDLIFTGTPEG 202

Query: 245 VGAFREPPLWMKPGDVVEVEISGL 268
           VGA  +       GD ++  + GL
Sbjct: 203 VGAVVK-------GDKLQGHVDGL 219


>gi|300310344|ref|YP_003774436.1| fumarylacetoacetate hydrolase
           family protein [Herbaspirillum seropedicae SmR1]
          Length = 327

 Score = 56.6 bits (135), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 46/158 (29%), Positives = 70/158 (44%), Gaps = 12/158 (7%)

Query: 94  PMIFARFADSQTGHLQPIIRPHASEKLDFEGELAVVIGRKARHIKAAEALDYVAGYACYN 153
           P+++   +D   G  Q +  P   + +DFEGE  V++G     ++A++AL  V      N
Sbjct: 111 PVMYQGASDDFLGPYQDVALPSEEDGIDFEGEFGVIVGPVPMGVQASQALQAVRLLVQIN 170

Query: 154 DGSVRDWQKHTIQ----FIPGKNFPQTGGFGPWLVTPDEAGDP-----SQLSLVTRLNGT 204
           D S+R    H ++    F+  K  P T  F P  VTPDE G        Q+ L  + NG 
Sbjct: 171 DWSLRALGPHEMKTGFGFLQAK--PST-AFAPMAVTPDELGPAWRDGRVQMRLQVQWNGQ 227

Query: 205 VVQEARTDDLIFPIPALIEYCSTFTELNPGDVIITGTT 242
                   ++ F    LI + +    L  G VI +GT 
Sbjct: 228 PFGHPHGGEMNFSFGELIAHAARSRRLTAGTVIGSGTV 265


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.319    0.138    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 877,649
Number of extensions: 34439
Number of successful extensions: 101
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 98
Number of HSP's successfully gapped: 3
Length of query: 279
Length of database: 1,619,870
Length adjustment: 84
Effective length of query: 195
Effective length of database: 1,222,130
Effective search space: 238315350
Effective search space used: 238315350
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 130 (54.7 bits)