BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0160.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter t... 291 1e-79 gi|300310481|ref|YP_003774573.1| major facilitator superfamily p... 221 1e-58 gi|300310645|ref|YP_003774737.1| major facilitator superfamily b... 196 4e-51 gi|300310615|ref|YP_003774707.1| major facilitator superfamily p... 110 2e-25 gi|300310666|ref|YP_003774758.1| major facilitator superfamily p... 101 1e-22 >gi|300310449|ref|YP_003774541.1| 4-hydroxybenzoate transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 447 Score = 291 bits (744), Expect = 1e-79, Method: Compositional matrix adjust. Identities = 170/435 (39%), Positives = 260/435 (59%), Gaps = 9/435 (2%) Query: 7 IQVSEVIDARPVGMVQWSAIGTCILIAVLDGFDTQTIGMLAPAMSRELGIPMARFGPIFS 66 + V +V++ G Q+ + C L ++DGFD Q +G +APA+ + G+ A GP+F Sbjct: 12 VHVDQVVEQGRFGAFQFGLLLLCGLCLIIDGFDVQAMGYVAPAIIADWGVSKASLGPVFG 71 Query: 67 AGLLGMLVGAMILGPLADRFGRKTMVIASTLAFGSMSLLTALAASHDDLLLLRFLTGIGL 126 AGL GML+G+++L P+ DR+GR+ ++I STL F L T + + D LL+LRF+TG GL Sbjct: 72 AGLFGMLLGSLVLTPVGDRYGRRPVLILSTLFFALCMLATPMVTTLDQLLVLRFITGFGL 131 Query: 127 GGALPNALALASEYSPRRHARVIVATLMCGMPLGAVLGGIVTSLLLPSHGWHAVFIVGGA 186 G +PNA+AL E+SP + + CG +GA GG V++ L+P++GWHAVF VGGA Sbjct: 132 GSIMPNAMALVGEFSPSSSRVTRMMLVSCGFTVGAAAGGFVSAALIPAYGWHAVFWVGGA 191 Query: 187 LPIAVALFALVFMPESARFLLVRGKHRARLQALMRRIAPELGADQ--DLAAPPPQAPGVP 244 +P+ + L LV++PES +FL++ + R ++ +R++ P + D ++ +A G+P Sbjct: 192 VPLVLGLAMLVWLPESIQFLVLHRRPRTQVARWLRKLDPNIVIDDKTEVVVKETKAEGMP 251 Query: 245 VAELFRHGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLMGAHHPVTVGITAISCFSAGGV 304 VA LFR GR TVLLW+ FMNL+ LYF+ +W+P ++ A + ++ + + GGV Sbjct: 252 VAALFRDGRAGVTVLLWLISFMNLINLYFLSNWLPTLIRDAGYSTSMAVLIGTSLQVGGV 311 Query: 305 LGSLLQGWLMNALGVRRTL--VLELALYALLALG-LANWPQSEWNVIVASSLMGVVIQGA 361 +G+L G + G R L LA ++ +G LA P + VA + G I G Sbjct: 312 IGTLSLGRFIQRFGFTRVLGSCFLLACLSIALIGKLATVPAL---LFVAVIVAGFCIVGG 368 Query: 362 QAGLNVLATEIYPTAMRATGIGFALGIGRIGSICGPLAGSLMLGWQMDVKHIFLMGITPA 421 Q +N LA YPT +R+TGIG+ALGIGRIGS+ GP+ G ++ Q +FL PA Sbjct: 369 QPAVNALAGTFYPTTLRSTGIGWALGIGRIGSVVGPVIGGQLIALQWTNASLFLAAAVPA 428 Query: 422 VIAGLAIV-FNRRAE 435 +I+ L I +R A+ Sbjct: 429 LISALTIARLHRTAQ 443 >gi|300310481|ref|YP_003774573.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 460 Score = 221 bits (562), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 134/377 (35%), Positives = 210/377 (55%), Gaps = 8/377 (2%) Query: 34 VLDGFDTQTIGMLAPAMSRELGIPMARFGPIFSAGLLGMLVGAMILGPLADRFGRKTMVI 93 V+DGFD Q IG P++ +E GI F P +AGL+GM +G+ G ADRFGR+ +I Sbjct: 39 VMDGFDGQLIGFAIPSLIKEWGITRNAFAPAVAAGLVGMGIGSAFAGLFADRFGRRMAII 98 Query: 94 ASTLAFGSMSLLTALAASHDDLLLLRFLTGIGLGGALPNALALASEYSP-RRHARVIVAT 152 S FG + L A + + +LRF G+G+GGALP++ + +E+SP RR + AT Sbjct: 99 GSVFVFGVATSLIAFSPDVTTIAILRFCAGLGIGGALPSSTTMTAEFSPARRRTLAVTAT 158 Query: 153 LMCGMPLGAVLGGIVTSLLLPSHGWHAVFIVGGALPIAVALFALVFMPESARFLLVRGKH 212 ++C +PLG +L G+ + +LP +GW +F++GG LP+A ++ +PES R+ L R H Sbjct: 159 IVC-VPLGGMLAGLFANAVLPHYGWRGLFLIGGLLPVAFSVLLWFVIPESPRY-LARHPH 216 Query: 213 R-ARLQALMRRIAPELGADQDLAAPPPQA--PGVPVAELFRHGRWKDTVLLWIPYFMNLV 269 R L+ L++R++ + AD A QA LF G+ +DT+ LW+ +FMNL+ Sbjct: 217 RWDELRRLLQRMSRPVSADTGFADAREQAIETRAGFGALFASGQARDTMALWVAFFMNLL 276 Query: 270 VLYFIVSWMPAVLMGAHHPVTVGITAISCFSAGGVLGSLLQGWLMNALGVRRTLVLELAL 329 +Y SW+P +L G +V ++ ++ GGV G+LL + G R ++L Sbjct: 277 AVYSAFSWLPTMLAGEGLSPSVAGAGLTAYNFGGVFGALLCALFITRFGSRLPMLLCCLA 336 Query: 330 YALLALGLANWPQSEWN--VIVASSLMGVVIQGAQAGLNVLATEIYPTAMRATGIGFALG 387 AL L + + ++V L G+ + Q+ + L IYPT +RATG AL Sbjct: 337 ATASALFLRSLDVASQTTLLVVGIGLHGLFVNAVQSTMYALCAYIYPTQVRATGTAAALA 396 Query: 388 IGRIGSICGPLAGSLML 404 GR+G+I AG+L++ Sbjct: 397 FGRLGAILSAFAGALVI 413 >gi|300310645|ref|YP_003774737.1| major facilitator superfamily benzoate permease [Herbaspirillum seropedicae SmR1] Length = 455 Score = 196 bits (497), Expect = 4e-51, Method: Compositional matrix adjust. Identities = 135/427 (31%), Positives = 229/427 (53%), Gaps = 11/427 (2%) Query: 7 IQVSEVIDARPVGMVQWSAIGTCILIAVLDGFDTQTIGMLAPAMSRELGIPMARFGPIFS 66 + VS++ID W + C L+ V DG+D G + P + +E + + G + S Sbjct: 4 LDVSQIIDGARFTKFHWMVMCLCALLLVFDGYDLFIYGAVLPVLMKEWSLSPVQAGALGS 63 Query: 67 AGLLGMLVGAMILGPLADRFGRKTMVIASTLAFGSMSLLTALAASHDDLLLLRFLTGIGL 126 L GM+ GA I GPLADR GRK V S + F + L+ A S + + RFL G+G Sbjct: 64 YALFGMMFGAFIFGPLADRIGRKKGVAISFVLFSVSTFLSGFATSPTEFGVYRFLAGLGC 123 Query: 127 GGALPNALALASEYSPRRHARVIVATLMCGMPLGAVLGGIVTSLLLPSHGWHAVFIVGGA 186 GG +PNA+AL +EY+P+R +VA + G LG VL + +LP+ GW ++F A Sbjct: 124 GGLMPNAVALMNEYAPKRLRSTLVAIMFSGYSLGGVLCAGLGIWMLPNFGWQSMFF-AAA 182 Query: 187 LPIAVALFALVFMPESARFLLVRGK-HRARLQALMRRIAPE--LGADQDLAAPPPQAPGV 243 +P+ + L +PES FLL +G+ +AR A++ ++ P+ + D L + G Sbjct: 183 VPLVLLPVILWKLPESVGFLLRQGRLEQAR--AMLTQVDPQARIATDTQLVQADVKGAGA 240 Query: 244 PVAELFRHGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLMGAHHPVTVGITAISCFSAGG 303 P+ LF+ R T+++W+ +F L+++Y + SW+P ++ A + + ++ + + GG Sbjct: 241 PMLHLFKQDRAVGTLMIWVSFFCCLLMVYALGSWLPKLMASAGYSLGSSLSFLLALNFGG 300 Query: 304 VLGSLLQGWLMNALGVRRTLV--LELALYALLALGLANWPQSEWNVIVASSLMGVVIQGA 361 V+G++ GWL + + + +V + A+ LG + + +I+ + G G Sbjct: 301 VVGAIGGGWLGDRFTLPKVVVSFFTVGTVAIALLGFNSPTPVLYLLIIVA---GATTIGT 357 Query: 362 QAGLNVLATEIYPTAMRATGIGFALGIGRIGSICGPLAGSLMLGWQMDVKHIFLMGITPA 421 Q L + YP A+R+TG+G+A G+GR G+I GPL G ++ + +K FL+ P Sbjct: 358 QILLYANTAQFYPLAIRSTGLGWASGVGRSGAIVGPLLGGALMAAALPLKMNFLVFAIPG 417 Query: 422 VIAGLAI 428 ++A LA+ Sbjct: 418 LVAALAM 424 >gi|300310615|ref|YP_003774707.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 478 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 114/377 (30%), Positives = 183/377 (48%), Gaps = 35/377 (9%) Query: 34 VLDGFDTQTIGMLAPAMSRELGIPMARFGPIFSAGLLGMLVGAMILGPLADRFGRKTMVI 93 + D F ++ + P + + A+ G + ++G LG ++GA+ G +A+R+GR V Sbjct: 54 LFDAFHALSLAFVLPVLVGLWQLSAAQIGLLIASGYLGQVIGALFFGWMAERYGR---VP 110 Query: 94 ASTLAFGSMSLLT---ALAASHDDLLLLRFLTGIGLGGALPNALALASEYSPRRHAR--- 147 ++++ G MS+L+ AL S L L RFL GIG+GG +P A +E S + H R Sbjct: 111 SASVTVGLMSVLSIVCALTGSFHMLFLCRFLQGIGVGGEVPVAATYINELS-QAHGRGKF 169 Query: 148 VIVATLMCGMPLGAVLGGIVTSLLLPSHGWHAVFIVGGALPIAVALFALVFMPESARFLL 207 ++ L+ PLG + V + L+P GW +F+VGG I VAL + +PES R+L+ Sbjct: 170 FVLYELI--FPLGLLAAAQVGAFLVPRFGWEYMFLVGGIPGILVALL-ITRLPESPRWLI 226 Query: 208 VRGK--HRARLQALMRRIAPE--LGADQDLAAPPPQAPGVPVAELFRH-GRWKD------ 256 RG+ +L + P+ L A D A + + R G WK+ Sbjct: 227 ARGRLDDAEKLIRTLEASTPKRNLDAQTDKQAIEQRINALAAGAGKRQRGSWKELFSPVY 286 Query: 257 ---TVLLWIPYFMNLVVLYFIVSWMPAVLMGAHH-PVTVGITAIS---CFSAGGVLGSLL 309 T+++W+ + + V I +W+P++ +H P+ + S SA VLG L Sbjct: 287 RGRTIIVWLLWASSYFVANGINNWLPSLYKTVYHLPLQESLRMASVSNVLSACAVLGCAL 346 Query: 310 QGWLMNALGVRRTLVLE-LALYALLALGLANWPQSEWNVIVASSLMGVVIQGAQAGLNVL 368 L++ +G RR + + ALLAL +S W+V+ +S V+ L + Sbjct: 347 ---LIDRVGRRRWAIASFILSGALLALLGVLKAESAWSVMFLASSAYAVMGTTTVLLYLY 403 Query: 369 ATEIYPTAMRATGIGFA 385 EIYPT MRA G G A Sbjct: 404 TPEIYPTRMRAIGTGLA 420 >gi|300310666|ref|YP_003774758.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 438 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 117/413 (28%), Positives = 191/413 (46%), Gaps = 36/413 (8%) Query: 3 SATPIQVSEVIDARPVGMVQWSAIGTCILIAVLDGFDTQTIGMLAPAMSRELG--IPMA- 59 S P V + + A VG Q+ L+ + D +IG AP +++ G +P A Sbjct: 2 STLPASVEQGLQAAGVGKFQYRLFVIFGLVWLADAMQVLSIGFSAPTIAKTFGLTVPQAL 61 Query: 60 RFGPIFSAGLLGMLVGAMILGPLADRFGRKTMVIASTLAFGSMSLLTALAASHDDLLLLR 119 + G +F +GML+GA + G LADR GR+ +++ + + + +A A LL LR Sbjct: 62 QTGTMF---FVGMLLGAFVFGRLADRIGRRPVLMGAVVIDACFGVASAFAPDFTWLLALR 118 Query: 120 FLTGIGLGGALPNALALASEYSPR-RHARVIV---ATLMCGMPLGAVLGGIVTSLLLPSH 175 LTGIG+GG LP + +E+ P R R +V + G AVL + S + Sbjct: 119 LLTGIGVGGTLPVDYTMMAEFLPSARRGRWLVMLESFWAIGTICLAVLALVAVS--WGND 176 Query: 176 GWHAVFIVGGALPIAVALFALVFMPESARFLLVRGKHRARLQALMRRIAPELGADQDLAA 235 W +F V G LP V + ++PES +L GK A Q L R+A G+ + Sbjct: 177 AWRVIFFVTG-LPALVGVVLRFYVPESPMYLNRSGKSDAARQVL-ERVAKVNGSSTPI-- 232 Query: 236 PPPQAPGV----PVAELFRHGRWKDTVLLWIPYFMNLVVLYFIVSWMPAVLMGAHHPVTV 291 PP P V P++ LF + ++ L++ + + + Y + ++P L Sbjct: 233 -PPLQPEVVERKPMSALFSAALRRRSLSLYLAWGLISIAYYGVFVYLPVKLGSEGFGFMR 291 Query: 292 GITAISCFSAGGVLGSLLQ--GWLMNALGVRR-----TLVLELALYALLALGLANWPQSE 344 G + + +L+Q G+ ++A GV R TLV L L A+ + L + + Sbjct: 292 GQIFL-------IFLALVQLPGYALSAYGVERWGRKPTLVGFLVLSAVGCM-LYSLGKDP 343 Query: 345 WNVIVASSLMGVVIQGAQAGLNVLATEIYPTAMRATGIGFALGIGRIGSICGP 397 V+ ++ L+ + G A L E+YPT +RA+G+G A + R G + P Sbjct: 344 MLVVGSTLLLSFSLLGTWAALYAFTPEVYPTDLRASGMGTAGAVARFGGLFAP 396 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,243,867 Number of extensions: 50167 Number of successful extensions: 462 Number of sequences better than 1.0e-08: 32 Number of HSP's gapped: 435 Number of HSP's successfully gapped: 33 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)