BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0158.p (492 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 285 6e-78 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 263 2e-71 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 246 3e-66 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 242 5e-65 gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrog... 237 2e-63 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 285 bits (729), Expect = 6e-78, Method: Compositional matrix adjust. Identities = 191/469 (40%), Positives = 266/469 (56%), Gaps = 11/469 (2%) Query: 20 GQWRDGTAASLLQDHDPYTGQLLTEIRQASLADLDAAYAAAAQAQQAWAAALPAERAALF 79 GQW AA +P TG+ LT++ AS D+ A+ A AQ AW A P ERA +F Sbjct: 19 GQWV--AAAQGATVLEPATGRPLTQVGLASPDDVSASVELAIAAQAAWVAMPPRERADIF 76 Query: 80 LRAAAIMDQRHTEIVDWLIRESGSTRIKAEIEWASVKSVMLAATALPGRVHGRILPIDIP 139 RAA + Q E+ + RE+G K E E ++ A +P + G++LP P Sbjct: 77 RRAAGLFHQHFDELARMVARETGGVLFKGEHEVREAITLCHLAAGMPLQAQGQLLP-STP 135 Query: 140 GRESRVYRRPLGVVGVISPWNWPMQLSNRSIAPALAVGNAVVVKPADDTPVTGGLLLAKI 199 GR S R PLGV+GVISP+N+P+ L+ R++APALA GNAVVVKP TPV+GG +LA+I Sbjct: 136 GRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPDLRTPVSGGFMLARI 195 Query: 200 YEEAGLPPGLLNIVVGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTMKRVG 259 +E+AGLP GLL+++ G +E G+A P I+FTGS VGR IG LA G +K++ Sbjct: 196 FEQAGLPAGLLHVLPGG-AETGEALVTAPGVPMIAFTGSPAVGRRIGELA--GRHLKKLS 252 Query: 260 LELGGNAPCVVLDDADLERAVDGAILGRFLHQGQICMSSNRIIVDASLYPRFVDAFVEKA 319 LELGG V+L+DADL+ A A G +LHQGQICM++NRI+V ASL + +KA Sbjct: 253 LELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILVHASLEEGLLQRLADKA 312 Query: 320 RALKHGNPNDPDTLIGPLINERQLQAAQGRLDAARQAGQRQVLGGEAEGLVLPPQVFVDV 379 R L G+ +GP+I+ERQLQ + + AG + V GG E L P V V Sbjct: 313 RHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLFYQPTVLGGV 372 Query: 380 TNDSPLAQAEQFAPIAPLIKAADEAEALHLANQTEYGLSSCVFTRDEARGVRFAQGIDAG 439 + + E F P+A +I+ ++ +A+ LAN+ GL++ V + R + A + G Sbjct: 373 RPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAGVLSPSVGRAMAVAARLRQG 432 Query: 440 MTHVNAISPCDDPNNMFGGEKNSGIGRF---NSDWIVAEMTMEHWVSIQ 485 M HVN + D+ N FGG +G G +DW E T W +I+ Sbjct: 433 MVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADW--EEYTQWRWTTIR 479 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 263 bits (673), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 164/473 (34%), Positives = 243/473 (51%), Gaps = 11/473 (2%) Query: 17 YIGGQWRDGTAASLLQDHDPYTGQLLTEIRQASLADLDAAYAAAAQAQQAWAAALPAERA 76 ++ GQW D L H+P G+LL + A+ A AA A AW ER+ Sbjct: 15 FVNGQWCDADQGERLAVHNPANGELLGHVPLMGAAETRRAIEAANAAWPAWKKKTAKERS 74 Query: 77 ALFLRAAAIMDQRHTEIVDWLIRESGSTRIKAEIEWASVKSVMLAATALPGRVHGRILPI 136 A+ R +M ++ + E G +A E S + R +G +P Sbjct: 75 AILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEEGKRTYGDTIPS 134 Query: 137 DIPGRESRVYRRPLGVVGVISPWNWPMQLSNRSIAPALAVGNAVVVKPADDTPVTGGLLL 196 P V + +GV I+PWN+P + R PALA G +V+KPA+ TP + L L Sbjct: 135 PSPSNRIVVIKEAIGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA-LAL 193 Query: 197 AKIYEEAGLPPGLLNIVVGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTMK 256 A + E AG+P G+ ++V G IG T +P + ISFTGST VG+ + + + ++K Sbjct: 194 AVLAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLL--MQQSASSIK 251 Query: 257 RVGLELGGNAPCVVLDDADLERAVDGAILGRFLHQGQICMSSNRIIVDASLYPRFVDAFV 316 ++ LELGGNAP +V DDADL+ AV+GAI ++ + GQ C+ +NRI V +Y F V Sbjct: 252 KLSLELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLV 311 Query: 317 EKARALKHGNPNDPDTLIGPLINERQLQAAQGRLDAARQAGQRQVLGGEAEGL---VLPP 373 E + K G + GPLINE+ +Q + + A G R +LGG+ L P Sbjct: 312 EAVKKFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEP 371 Query: 374 QVFVDVTNDSPLAQAEQFAPIAPLIKAADEAEALHLANQTEYGLSSCVFTRDEARGVRFA 433 V DVT +A+ E F P+APL + + E L LAN TE+GL+S ++RD R R A Sbjct: 372 TVLADVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVA 431 Query: 434 QGIDAGMTHVNA--ISPCDDPNNMFGGEKNSGIGRFNSDWIVAEMTMEHWVSI 484 +G+++GM +N IS P FGG K SG+GR S + + + + ++ + Sbjct: 432 EGLESGMVGINTGLISNEVAP---FGGVKQSGLGREGSHYGIDDYLVVKYLCM 481 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 246 bits (628), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 169/457 (36%), Positives = 234/457 (51%), Gaps = 17/457 (3%) Query: 17 YIGGQWRDGTAASLLQDHDPYTGQLLTEIRQASLADLDAAYAAAAQAQQAWAAALPAERA 76 YI G+W+ + P T + + EI + AD+D A AA +A AWAA P ERA Sbjct: 19 YIDGKWQPPQGSERAAVVAPATEERVCEIALGNAADVDQAVQAARRAIPAWAATSPLERA 78 Query: 77 ALFLRAAAIMDQRHTEIVDWLIRESGSTRIKAEIEWASVKSVMLAATALP-GRVHGRILP 135 AL R A++ +R L E G A I +A V LAA L R + P Sbjct: 79 ALLGRVHALLLERSELFAQALTLEMG-----APISYARTAHVPLAAEHLRVARDNLADYP 133 Query: 136 IDIPGRESRVYRRPLGVVGVISPWNWPMQLSNRSIAPALAVGNAVVVKPADDTPVTGGLL 195 P + + R P+GV +I+PWNWP+ + PALA G VV+KP++ +P++ LL Sbjct: 134 FIRPRGTTAIVREPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA-LL 192 Query: 196 LAKIYEEAGLPPGLLNIVVGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTM 255 A+I EAG+P G+ N+V G+ +E+G A + HP IS TGSTR G + A T+ Sbjct: 193 FAEIVHEAGVPAGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAV--TV 250 Query: 256 KRVGLELGGNAPCVVLDDADLERAVDGAILGRFLHQGQICMSSNRIIVDASLYPRFVDAF 315 KRV ELGG +P +VL DADLERA+ + + GQ C + R+IV + R + Sbjct: 251 KRVAQELGGKSPNLVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELA 310 Query: 316 VEKARALKHGNPNDPDTLIGPLINERQLQAAQGRLDAARQAGQRQVLGGE------AEGL 369 + +K G+PND T GPL N Q Q ++A Q G R + GG A+G Sbjct: 311 LATLAQMKVGDPNDAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGY 370 Query: 370 VLPPQVFVDVTNDSPLAQAEQFAPIAPLIKAADEAEALHLANQTEYGLSSCVFTRDEARG 429 P +F +V D +AQ E F P+ ++ EA+ +AN T YGL + V RD+ + Sbjct: 371 YARPTIFSEVHTDMVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQV 430 Query: 430 VRFAQGIDAGMTHVNAISPCDDPNNMFGGEKNSGIGR 466 A I +G H+N P DP FGG K SG GR Sbjct: 431 RAVAARIQSGQVHLN--YPAWDPQAPFGGYKQSGNGR 465 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 242 bits (618), Expect = 5e-65, Method: Compositional matrix adjust. Identities = 171/472 (36%), Positives = 255/472 (54%), Gaps = 6/472 (1%) Query: 18 IGGQWRDGTAASLLQDHDPYTGQLLTEIRQASLADLDAAYAAAAQAQQAWAAALPAERAA 77 I G+ R T A+ + +P + A++AD AA AAAA A AWAA P +R A Sbjct: 8 INGERRGATGAAYFERRNPLDHSVAARAPAATVADARAAVAAAAAAFPAWAAIGPGQRRA 67 Query: 78 LFLRAAAIMDQRHTEIVDWLIRESGSTRIKAEIEWASVKSVMLAATALPGRVHGRILPID 137 L AA + R + + E+G++ A + A A+ ++ G ++P D Sbjct: 68 LLTHAADALQARADDFAQAMAAETGASHHWAGFNVKLAADGLREAAAMTTQIAGEVIPSD 127 Query: 138 IPGRESRVYRRPLGVVGVISPWNWPMQLSNRSIAPALAVGNAVVVKPADDTPVTGGLLLA 197 +PG + R+P GVV I+PWN P+ L R+IA LA GN VV K ++ P T GL++A Sbjct: 128 VPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGNTVVFKGSEICPATHGLIIA 187 Query: 198 KIYEEAGLPPGLLNIVVGAVSEIGDAFTLH---PTPKFISFTGSTRVGRHIGALAMTGPT 254 + +EAGLPPG++N V A + P + ++FTGSTRVGR I A Sbjct: 188 AL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNFTGSTRVGRIIAA--QCARH 244 Query: 255 MKRVGLELGGNAPCVVLDDADLERAVDGAILGRFLHQGQICMSSNRIIVDASLYPRFVDA 314 +K LELGG AP +VL DADL+ AV+ A G F + GQICMS+ RIIVD S+ FV Sbjct: 245 LKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMSTERIIVDDSVADEFVRR 304 Query: 315 FVEKARALKHGNPNDPDTLIGPLINERQLQAAQGRLDAARQAGQRQVLGGEAEGLVLPPQ 374 KA +L G+P ++G +++ ++ +D A G V GG A ++P Sbjct: 305 LAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKGATLVCGGRASDTLMPAT 364 Query: 375 VFVDVTNDSPLAQAEQFAPIAPLIKAADEAEALHLANQTEYGLSSCVFTRDEARGVRFAQ 434 + V+ + + + E F P+ P+++ A + A+ AN EYGLSS +F+RD AR + ++ Sbjct: 365 LLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGLSSALFSRDVARALALSR 424 Query: 435 GIDAGMTHVNAISPCDDPNNMFGGEKNSGIGRFNSDWIVAEMTMEHWVSIQS 486 I++G+ H+N + D+ FGG K+SG G F +AE T WV++Q+ Sbjct: 425 RIESGICHINGPTVHDEAQMPFGGVKSSGFGHFGGKAGIAEFTELRWVTVQT 476 >gi|300310772|ref|YP_003774864.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 237 bits (605), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 144/452 (31%), Positives = 234/452 (51%), Gaps = 7/452 (1%) Query: 17 YIGGQWRDGTAASLLQDHDPYTGQLLTEIRQASLADLDAAYAAAAQAQQAWAAALPAERA 76 +I G+W + + +P T +++ +I A ADLD A AA++ + W ER+ Sbjct: 8 FINGEWTASASGRTIDVINPATEEVIGKIAHADRADLDRALEAASKGFETWRNMSAFERS 67 Query: 77 ALFLRAAAIMDQRHTEIVDWLIRESGSTRIKAEIEWASVKSVMLAATALPGRVHGRILPI 136 + RAA ++ +R E+ + E G +A++E + R +GR++P Sbjct: 68 KIMRRAADLLRERAEEVARLMTMEQGKPLAEAKMETLGAADTIDWFAEEARRTYGRLVPA 127 Query: 137 DIPGRESRVYRRPLGVVGVISPWNWPMQLSNRSIAPALAVGNAVVVKPADDTPVTGGLLL 196 +PG V + P+G V +PWN+P+ R ++ ALA G +++VK ++TP + L+ Sbjct: 128 RVPGAYQMVIKEPVGPVAAFTPWNFPLNQVVRKLSAALAAGCSIIVKAPEETPASPAELI 187 Query: 197 AKIYEEAGLPPGLLNIVVGAVSEIGDAFTLHPTPKFISFTGSTRVGRHIGALAMTGPTMK 256 + Y +AG+P G++N+V G +EI + HP K ISFTGST VG+ + ALA G MK Sbjct: 188 -RAYADAGVPAGVINLVYGVPAEISEYLIPHPVIKKISFTGSTPVGKQLAALA--GQHMK 244 Query: 257 RVGLELGGNAPCVVLDDADLERAVDGAILGRFLHQGQICMSSNRIIVDASLYPRFVDAFV 316 R +ELGG+AP +V DDAD++ A +F + GQ+C+S R +V ++ FVD FV Sbjct: 245 RATMELGGHAPAMVFDDADVDVAAKMLATAKFRNAGQVCVSPTRFLVQKGVFNAFVDKFV 304 Query: 317 EKARALKHGNPNDPDTLIGPLINERQLQAAQGRLDAARQAGQRQVLGGE---AEGLVLPP 373 A LK GN + +GP+ N+R++ A + +D A G + GG+ +G P Sbjct: 305 TYAEQLKVGNGLEAGITMGPVANKRRIPALEALIDDAVSQGAQLRTGGKRIGNKGYFFQP 364 Query: 374 QVFVDVTNDSPLAQAEQFAPIAPLIKAADEAEALHLANQTEYGLSSCVFTRDEARGVRFA 433 V ++ + E F P+A + E + AN+ YGL++ F + A Sbjct: 365 TVLTNLPLQARAMNEEPFGPLALINSFETLDEVITEANRLPYGLAAYAFAKSAHTKHALA 424 Query: 434 QGIDAGMTHVNAISPCDDPNNMFGGEKNSGIG 465 +++GM +N + P FGG K+SG G Sbjct: 425 ARVESGMLTINHLG-LALPELPFGGIKDSGYG 455 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,580,290 Number of extensions: 69652 Number of successful extensions: 309 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 235 Number of HSP's successfully gapped: 20 Length of query: 492 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 403 Effective length of database: 1,198,455 Effective search space: 482977365 Effective search space used: 482977365 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)