BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0156.p (373 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 391 e-110 gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxi... 259 2e-70 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 238 9e-64 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 229 3e-61 gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrog... 226 2e-60 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 391 bits (1004), Expect = e-110, Method: Compositional matrix adjust. Identities = 198/374 (52%), Positives = 270/374 (72%), Gaps = 2/374 (0%) Query: 1 LFHSAA-LFIEPEGHVLRSANSGRLSIARRVPLGVVGVITPWNFPLLLAMRSVAPALALG 59 L H AA + ++ +G +L S GRLSIARR PLGV+GVI+P+NFPL+L +R+VAPALA G Sbjct: 115 LCHLAAGMPLQAQGQLLPS-TPGRLSIARRAPLGVIGVISPFNFPLILTLRTVAPALAAG 173 Query: 60 NAVVLKPDPQTPITGGVVLAQIFAEAGLPEGVFQIVNGGADVGEALVGAPAVRLVTFTGS 119 NAVV+KPD +TP++GG +LA+IF +AGLP G+ ++ GGA+ GEALV AP V ++ FTGS Sbjct: 174 NAVVVKPDLRTPVSGGFMLARIFEQAGLPAGLLHVLPGGAETGEALVTAPGVPMIAFTGS 233 Query: 120 TRVGRRVGELAGQHVKKVSLELGGKSPFIVLDDADLDAAASAGAWASFLHQGQICMASGR 179 VGRR+GELAG+H+KK+SLELGG + ++L+DADLD AAS AW ++LHQGQICMA+ R Sbjct: 234 PAVGRRIGELAGRHLKKLSLELGGANALVILEDADLDVAASHAAWGAWLHQGQICMAANR 293 Query: 180 HIVLRSVAKTYLELLRDHTERLAVGNPFEENVAIGPIINRRQIERIHDLVTSSVASGAKI 239 +V S+ + L+ L D L VG+ VA+GP+I+ RQ++R+H LV SVA+GA++ Sbjct: 294 ILVHASLEEGLLQRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQL 353 Query: 240 ITGGTYAHPYYRPTVLSGVTPGMAAYEQEIFGPVAPVVIAENEEHAIQLANDTEYGLSAA 299 + GG Y +Y+PTVL GV PGM +E+EIFGPVA V+ +N+E AI LAN GL+A Sbjct: 354 VAGGRYEQLFYQPTVLGGVRPGMRVFEEEIFGPVASVIRFDNDEDAIALANRHAGGLAAG 413 Query: 300 VQTASLERGLRVAQRIRAGMVHINDQTISDLPGIPMGGMGQSGNGSRFGSTTNVDEFTEW 359 V + S+ R + VA R+R GMVH+NDQT++D P GG G +GNG+ G + +E+T+W Sbjct: 414 VLSPSVGRAMAVAARLRQGMVHVNDQTVNDECVNPFGGPGIAGNGASVGGPADWEEYTQW 473 Query: 360 QWMTIGATPTRYAF 373 +W TI Y F Sbjct: 474 RWTTIRQQAMPYPF 487 >gi|300310482|ref|YP_003774574.1| vanillin: NAD dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 483 Score = 259 bits (662), Expect = 2e-70, Method: Compositional matrix adjust. Identities = 156/377 (41%), Positives = 222/377 (58%), Gaps = 6/377 (1%) Query: 1 LFHSAALFIEPEGHVLRSANSGRLSIARRVPLGVVGVITPWNFPLLLAMRSVAPALALGN 60 L +AA+ + G V+ S G L++ R P GVV I PWN P++L +R++A LA GN Sbjct: 109 LREAAAMTTQIAGEVIPSDVPGNLAMGVRQPAGVVLGIAPWNAPVILGVRAIALPLACGN 168 Query: 61 AVVLKPDPQTPITGGVVLAQIFAEAGLPEGVFQIVNGGAD----VGEALVGAPAVRLVTF 116 VV K P T G+++A + EAGLP GV V + + EA++ PAVR V F Sbjct: 169 TVVFKGSEICPATHGLIIAAL-QEAGLPPGVVNFVTNAPEDAAAIVEAIIAEPAVRRVNF 227 Query: 117 TGSTRVGRRVGELAGQHVKKVSLELGGKSPFIVLDDADLDAAASAGAWASFLHQGQICMA 176 TGSTRVGR + +H+K LELGGK+PFIVL DADLDAA A A+ +F + GQICM+ Sbjct: 228 TGSTRVGRIIAAQCARHLKPAVLELGGKAPFIVLHDADLDAAVEAAAFGAFANSGQICMS 287 Query: 177 SGRHIVLRSVAKTYLELLRDHTERLAVGNPFEENVAIGPIINRRQIERIHDLVTSSVASG 236 + R IV SVA ++ L L +G+P + V +G +++ +ER + L+ ++A G Sbjct: 288 TERIIVDDSVADEFVRRLAAKAASLPLGDPRQGPVVLGSVVDMATVERCNALIDDALAKG 347 Query: 237 AKIITGGTYAHPYYRPTVLSGVTPGMAAYEQEIFGPVAPVVIAENEEHAIQLANDTEYGL 296 A ++ GG + T+L V+ M Y +E FGPV P+V +E AI AND EYGL Sbjct: 348 ATLVCGGRASDTLMPATLLDHVSANMLIYREESFGPVKPIVRVAGDEAAIACANDNEYGL 407 Query: 297 SAAVQTASLERGLRVAQRIRAGMVHINDQTISDLPGIPMGGMGQSGNGSRFGSTTNVDEF 356 S+A+ + + R L +++RI +G+ HIN T+ D +P GG+ SG G FG + EF Sbjct: 408 SSALFSRDVARALALSRRIESGICHINGPTVHDEAQMPFGGVKSSGFG-HFGGKAGIAEF 466 Query: 357 TEWQWMTIGATPTRYAF 373 TE +W+T+ TP Y F Sbjct: 467 TELRWVTVQTTPRHYPF 483 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 238 bits (606), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 134/349 (38%), Positives = 201/349 (57%), Gaps = 8/349 (2%) Query: 21 SGRLSIARRVPLGVVGVITPWNFPLLLAMRSVAPALALGNAVVLKPDPQTPITGGVVLAQ 80 S R+ + + +GV ITPWNFP + R PALA G +VLKP TP + + LA Sbjct: 138 SNRIVVIKEA-IGVCAAITPWNFPAAMITRKAGPALAAGCPMVLKPAEATPFSA-LALAV 195 Query: 81 IFAEAGLPEGVFQIVNGGAD-VGEALVGAPAVRLVTFTGSTRVGRRVGELAGQHVKKVSL 139 + AG+P GVF +V G +G + P VR ++FTGST VG+ + + + +KK+SL Sbjct: 196 LAERAGIPAGVFSVVTGTPKGIGGEMTSNPIVRKISFTGSTGVGKLLMQQSASSIKKLSL 255 Query: 140 ELGGKSPFIVLDDADLDAAASAGAWASFLHQGQICMASGRHIVLRSVAKTYLELLRDHTE 199 ELGG +PFIV DDADLDAA + + + GQ C+ + R V V + L + + Sbjct: 256 ELGGNAPFIVFDDADLDAAVEGAIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVK 315 Query: 200 RLAVGNPFEENVAIGPIINRRQIERIHDLVTSSVASGAKIITGGT---YAHPYYRPTVLS 256 + VG EE V GP+IN + ++++ V +VA GA+++ GG H ++ PTVL+ Sbjct: 316 KFKVGQGTEEGVTQGPLINEQAVQKVEQHVADAVAKGARVLLGGKRHALGHSFFEPTVLA 375 Query: 257 GVTPGMAAYEQEIFGPVAPVVIAENEEHAIQLANDTEYGLSAAVQTASLERGLRVAQRIR 316 VTP M +E FGP+AP+ + +E + LANDTE+GL++ + + R RVA+ + Sbjct: 376 DVTPAMQVAREETFGPMAPLFRFKTDEEVLALANDTEFGLASYFYSRDIGRIWRVAEGLE 435 Query: 317 AGMVHINDQTISDLPGIPMGGMGQSGNGSRFGSTTNVDEFTEWQWMTIG 365 +GMV IN IS+ P GG+ QSG G R GS +D++ +++ +G Sbjct: 436 SGMVGINTGLISNEVA-PFGGVKQSGLG-REGSHYGIDDYLVVKYLCMG 482 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 229 bits (584), Expect = 3e-61, Method: Compositional matrix adjust. Identities = 140/337 (41%), Positives = 193/337 (57%), Gaps = 11/337 (3%) Query: 29 RVPLGVVGVITPWNFPLLLAMRSVAPALALGNAVVLKPDPQTPITGGVVLAQIFAEAGLP 88 R P+GV +ITPWN+P+ V PALA G VVLKP +P++ ++ A+I EAG+P Sbjct: 145 REPIGVCALITPWNWPIYQITAKVGPALAAGCTVVLKPSELSPLSA-LLFAEIVHEAGVP 203 Query: 89 EGVFQIVNG-GADVGEALVGAPAVRLVTFTGSTRVGRRVGELAGQHVKKVSLELGGKSPF 147 GVF +VNG GA+VG AL P V +++ TGSTR G V + A VK+V+ ELGGKSP Sbjct: 204 AGVFNLVNGSGAEVGAALSAHPQVDMISITGSTRAGVLVAQAAAVTVKRVAQELGGKSPN 263 Query: 148 IVLDDADLDAAASAGAWASFLHQGQICMASGRHIVLRSVAKTYLELLRDHTERLAVGNPF 207 +VL DADL+ A G A+ + GQ C A R IV R+ EL ++ VG+P Sbjct: 264 LVLPDADLERAIPPGVAAALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMKVGDPN 323 Query: 208 EENVAIGPIINRRQIERIHDLVTSSVASGAKIITGGT-----YAHPYY-RPTVLSGVTPG 261 + GP+ NR Q R+ ++ + + GA+++ GG A YY RPT+ S V Sbjct: 324 DAATTHGPLANRAQFLRVQQMIEAGLQDGARLLAGGPGRPDGLAQGYYARPTIFSEVHTD 383 Query: 262 MAAYEQEIFGPVAPVVIAENEEHAIQLANDTEYGLSAAVQTASLERGLRVAQRIRAGMVH 321 M ++EIFGPV ++ + E AI +ANDT YGL A VQ ++ VA RI++G VH Sbjct: 384 MVIAQEEIFGPVLAILPYDTVEEAIAIANDTVYGLGAHVQGRDKDQVRAVAARIQSGQVH 443 Query: 322 INDQTISDLPGIPMGGMGQSGNGSRFGSTTNVDEFTE 358 +N P P GG QSGNG +G ++E+ E Sbjct: 444 LNYPAWD--PQAPFGGYKQSGNGREYG-VEGMEEYME 477 >gi|300313241|ref|YP_003777333.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 226 bits (577), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 136/346 (39%), Positives = 200/346 (57%), Gaps = 9/346 (2%) Query: 24 LSIARRVPLGVVGVITPWNFPLLLAMRSVAPALALGNAVVLKPDPQTPITGGVVLAQIFA 83 LS R P+GVVG I PWN PL++ VA LA G VV+KP +TP + + LA++ Sbjct: 139 LSYTVREPIGVVGAILPWNAPLMITSWKVAALLAAGCTVVIKPAEETPQSA-LHLARLAQ 197 Query: 84 EAGLPEGVFQIVNG-GADVGEALVGAPAVRLVTFTGSTRVGRRVGELAGQHVKKVSLELG 142 EAG P+GV +V G G VG AL P V ++FTGS GR + +AG+ K+V+LELG Sbjct: 198 EAGFPDGVINVVTGYGNSVGRALCEHPDVAKISFTGSPEAGRAIQRIAGEQFKRVTLELG 257 Query: 143 GKSPFIVLDDADLDAAASAGAWASFLHQGQICMASGRHIVLRSVAKTYLELLRDHTERLA 202 GKSP IV DDA + A F++QGQ+C A R +V RS+A+ + + L + + Sbjct: 258 GKSPQIVFDDAPFEDALFGCTLGLFVNQGQVCAAGSRILVQRSLAQRFAKALAEAAAGIT 317 Query: 203 VGNPFEENVAIGPIINRRQIERIHDLVTSSVASGAKIITGGTYAHP----YYRPTVLSGV 258 VG+P + V +GP+ + Q ER++ + + GA ++ GG + P + +PT+ + Sbjct: 318 VGDPSQPGVRMGPVAKKAQFERVNRYIQQGLDEGATLLAGGV-SRPDKGWFVQPTIFADA 376 Query: 259 TPGMAAYEQEIFGPVAPVVIAENEEHAIQLANDTEYGLSAAVQTASLERGLRVAQRIRAG 318 GM+ +EIFGPV ++ + EE AI LAND++YGL+A V T +L R RVA ++ G Sbjct: 377 NNGMSIAREEIFGPVGTIISFDTEEEAIALANDSKYGLAATVWTTNLARAHRVAAGVKVG 436 Query: 319 MVHINDQTISDLPGIPMGGMGQSGNGSRFGSTTNVDEFTEWQWMTI 364 V +N + D +P GG+ SG G R G +TE + +T+ Sbjct: 437 AVGVNCWSPLD-ANLPWGGIKDSGMG-REGGLAGALAYTEEKTVTV 480 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,255,733 Number of extensions: 56668 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 20 Length of query: 373 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 286 Effective length of database: 1,207,925 Effective search space: 345466550 Effective search space used: 345466550 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)