BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0152.p (257 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogena... 174 9e-45 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 149 2e-37 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 126 2e-30 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 119 3e-28 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 117 1e-27 >gi|300311869|ref|YP_003775961.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 174 bits (440), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 109/254 (42%), Positives = 142/254 (55%), Gaps = 9/254 (3%) Query: 7 KVAFVTGASRGIGREIATRLAANGATVAIHYGGNLQAAQETLATIEQTGGRAFVIQADLH 66 ++A +TG SRG+G+ A LA +GA V + Y GN QAA+ T+A ++ G A ++ DL Sbjct: 9 RIALITGGSRGLGKNTALHLARDGAGVILTYVGNQQAAEATVAELQALGAVAAALRLDLA 68 Query: 67 SSDASFAATFEEFDRSLLTCSGQTGLDILVNNAGQIMFGSVEVLSSAEFDKAFAVDVKAP 126 S ASF A + +L T LVNN G M S+E S A FD F V +K P Sbjct: 69 DS-ASFPAFAQAVREALKTHWQAEHFHYLVNNGGMGMHASIEETSEAVFDALFKVHLKGP 127 Query: 127 FFLTQAALPRLRDGGRIINISSGTSRIVNPNVVSYAMAKGALEVFSKTAALQLGARGITV 186 FFLTQA LP L GGRIINISSG +R P +YA KG +EV ++ A + GARGI V Sbjct: 128 FFLTQALLPLLAQGGRIINISSGLARFSLPGYAAYASMKGGVEVMTRYMAKEFGARGIVV 187 Query: 187 NAVAPGPTAT----AEFLRMAEGNPAFIEHAKAQSALRRLGTPEDIANIVAFLASDEGGW 242 N VAPG T AE N +F+ H Q+AL R G P+DI +A L + GW Sbjct: 188 NTVAPGAIETDFGGGRVRDNAELN-SFVAH---QTALGRAGLPDDIGAAIASLLRPDNGW 243 Query: 243 ITGQVIDATGGSLL 256 I Q ++ +GG + Sbjct: 244 INAQRVEVSGGMFI 257 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 149 bits (376), Expect = 2e-37, Method: Compositional matrix adjust. Identities = 108/254 (42%), Positives = 142/254 (55%), Gaps = 18/254 (7%) Query: 4 LKGKVAFVTGASRGIGREIATRLAANGATVAIHYGGNLQAAQETLATIEQTGGRAFVIQA 63 L KVAFV G SRGIG I RLAA GATVA Y + AQ +++++ GGRA IQA Sbjct: 15 LVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALAIQA 74 Query: 64 DLHSSDASFAATFEEFDRSLLTCSGQTGLDILVNNAGQIMFGSVEVLSSAEFDKAFAVDV 123 D S+D AA ++ R G+ LDILVNNAG + G ++ + D+ AV+V Sbjct: 75 D--SAD---AAALQQAIRLAAETFGR--LDILVNNAGVLAMGPLDQFKLEDLDRTLAVNV 127 Query: 124 KAPFFLTQAALPRLRDGGRIINI-SSGTSRIVNPNVVSYAMAKGALEVFSKTAALQLGAR 182 ++ F TQ A + +GGR+I+I S+ RI YAM+K AL +K A LG R Sbjct: 128 RSVFVATQEAARYMGEGGRVISIGSTNADRIPFAGGAVYAMSKSALVGLTKGLARDLGPR 187 Query: 183 GITVNAVAPGPTATAEFLRMAEGNPAFIEHAKAQS---ALRRLGTPEDIANIVAFLASDE 239 GITVN V PGP T + NPA + A + + AL R G E+IA+ VA+LA E Sbjct: 188 GITVNNVQPGPVDT-------DMNPASGDFAASLTGLMALPRYGKAEEIASFVAYLAGPE 240 Query: 240 GGWITGQVIDATGG 253 G+ITG + GG Sbjct: 241 SGYITGANLMIDGG 254 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 126 bits (316), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 88/258 (34%), Positives = 141/258 (54%), Gaps = 18/258 (6%) Query: 4 LKGKVAFVTGASRGIGREIATRLAANGATVAIHYGGNLQAAQETLATIEQTGGRAFVIQA 63 L GKVA +TG GIGR +A A GA +AI Y + A++T ++ ++ Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 64 DLHSSDASFAATFEEFDRSLLTCSGQTGLDILVNNAGQIMFG-SVEVLSSAEFDKAFAVD 122 D+ S + +A R+++ G+ LD+LVNNA Q S+E +S A+ + F + Sbjct: 183 DVGSPEFCRSAV-----RAVMDAYGR--LDVLVNNAAQQYPAQSIEEISQAQLEHTFRTN 235 Query: 123 VKAPFFLTQAALPRLRDGGRIINISSGTSRIVNPNVVSYAMAKGALEVFSKTAALQLGAR 182 + + F++ + A+P L G RIIN +S T+ + ++V YA KGA+ F+++ + QL + Sbjct: 236 IFSMFYMVKEAMPFLTRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASA 295 Query: 183 GITVNAVAPGPTAT----AEFLRMAEGNPAFIEHAKAQSALRRLGTPEDIANIVAFLASD 238 GI VNAVAPGP T A F AE +F ++ L R G P+++A FLA++ Sbjct: 296 GIHVNAVAPGPIWTPLIPASF--SAEEVASFGKNVP----LGRPGQPDEVAPAYVFLATE 349 Query: 239 EGGWITGQVIDATGGSLL 256 ++TGQV+ GG ++ Sbjct: 350 GASYMTGQVLHPNGGEIV 367 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 119 bits (297), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 99/260 (38%), Positives = 134/260 (51%), Gaps = 18/260 (6%) Query: 3 DLKGKVAFVTGASRGIGREIATRLAANGATV---AIHYGGNLQAAQETLATIEQTGGRAF 59 +L+G+VA VTGASRGIGR IAT LA GA V A G A E LA + GR Sbjct: 5 NLQGQVALVTGASRGIGRAIATELARQGAKVIGTATSESG-AAAITEYLAALGPEAGRGA 63 Query: 60 VIQADLHSSDASFAATFEEFDRSLLTCSGQTGLDILVNNAGQIMFGSVEVLSSAEFDKAF 119 V+ +++ ++AS A E+ + + L ILVNNAG + E+D Sbjct: 64 VL--NVNDAEASVA-LVEQVQKDFGS------LSILVNNAGITQDQLAMRMKDEEWDSVI 114 Query: 120 AVDVKAPFFLTQAALPRLRDG--GRIINISSGTSRIVNPNVVSYAMAKGALEVFSKTAAL 177 A ++ A L++A L + GRIINI+S NP ++YA AK + S+ A Sbjct: 115 ATNLTAVGRLSRAVLRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAR 174 Query: 178 QLGARGITVNAVAPGPTATAEFLRMAEGNPAFIEHAKAQSALRRLGTPEDIANIVAFLAS 237 ++G+R ITVN VAPG T + E A I Q L RLG E+IA VAFLAS Sbjct: 175 EIGSRNITVNCVAPGFIDTDMTRALNEQQSAAILQ---QIPLGRLGAAEEIAYAVAFLAS 231 Query: 238 DEGGWITGQVIDATGGSLLG 257 + +ITG ++ GG +G Sbjct: 232 PQAAYITGTTLNVNGGMYMG 251 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 117 bits (293), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 82/257 (31%), Positives = 132/257 (51%), Gaps = 14/257 (5%) Query: 4 LKGKVAFVTGASRGIGREIATRLAANGATVAIHYGGNLQAAQETLATIEQTGGRAFVIQA 63 + ++A+VTG GIG I TRL +G TV G N + LAT+ + G Sbjct: 1 MSKRIAYVTGGMGGIGTAICTRLCKDGYTVVAGCGPNSTRKDKWLATMREQG-------F 53 Query: 64 DLHSSDASFAATFEEFDRSLLTCSGQTG-LDILVNNAGQIMFGSVEVLSSAEFDKAFAVD 122 D+H+S+ + + +E + + G +D+LVNNAG G +S ++D + Sbjct: 54 DIHASEGN-VSNWESTKAAFEKVRAEIGEVDVLVNNAGITRDGQFRKMSKDDWDAVIDTN 112 Query: 123 VKAPFFLTQAALPRL--RDGGRIINISSGTSRIVNPNVVSYAMAKGALEVFSKTAALQLG 180 + + F +T+ + + R GRIINISS + +Y+ AK + F+ A ++ Sbjct: 113 LNSLFNVTKQVIEGMCERGFGRIINISSVNGQKGQFGQTNYSTAKAGIHGFTMALAQEVA 172 Query: 181 ARGITVNAVAPGPTATAEFLRMAEGNPAFIEHAKAQSALRRLGTPEDIANIVAFLASDEG 240 +G+TVN V+PG T + +R P +E + ++RLG PE+IA+IVA++AS EG Sbjct: 173 TKGVTVNTVSPGYIGT-DMVRAIR--PEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEG 229 Query: 241 GWITGQVIDATGGSLLG 257 G+ TG GG +G Sbjct: 230 GYATGSDFSLNGGLYMG 246 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.368 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 797,189 Number of extensions: 32536 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 46 Length of query: 257 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 174 Effective length of database: 1,226,865 Effective search space: 213474510 Effective search space used: 213474510 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)