BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0149.p
         (356 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300311873|ref|YP_003775965.1| poly(3-hydroxybutyrate) depolym...    68   1e-12

>gi|300311873|ref|YP_003775965.1| poly(3-hydroxybutyrate)
           depolymerase [Herbaspirillum seropedicae SmR1]
          Length = 369

 Score = 68.2 bits (165), Expect = 1e-12,   Method: Compositional matrix adjust.
 Identities = 70/277 (25%), Positives = 114/277 (41%), Gaps = 41/277 (14%)

Query: 87  PLIVMLHGCQQNAADFAQGTRMNAHAAKHGFAVLYPEQSLRAHPHRCWR-----WYAQDA 141
           PLI+ LH    + +  A+ T +   A   G+ + YP  +  A   R W       YAQ  
Sbjct: 74  PLIIALHASGSSGSLMARATGLTEIAEAAGYMIAYPNGTGLAIDARTWNSGGCCGYAQ-- 131

Query: 142 QKGEGDVALVASLIQEILKTHPIDPQRIYVCGISAGAALAHLLALTHPELIAAVALHAAP 201
                DVA + +L++++ K    DPQR+Y+ G+S G  +A+ +A   P L  AVA+ +A 
Sbjct: 132 MHKVDDVAFLRALVEKLAKDGLADPQRVYLVGVSNGGMMAYRMAAEAPGLFKAVAVVSAV 191

Query: 202 VSGVCSNAAGAYSVMQ-HGSLRA-----AAAAKRSLSRFPRTGALPAILIGGTDDKIVRP 255
           +            V+  HGS            K+ +S+ PR                   
Sbjct: 192 LDVPPETVKAPMPVLHIHGSEDPFIPFLGGIGKQEVSQLPR------------------- 232

Query: 256 VNQRQLLEQFLLLNHTQSLTEQPPRETPYGRTSKKQPRRRIVATRDYVRGRKLIIRSVLI 315
           ++  + +E ++  N      E  P  T    T+      R    R     + +++  V  
Sbjct: 233 LSVARTIETWVKANG----AEPRPAVTDIADTANDGTTIRQYTYRSKTDPQSVVLYEV-- 286

Query: 316 EGLGHAWSGGDTTL---SYNAAGPDASRLVVDFFSRH 349
           +G GHAW G    +     ++   DASR VV+FF+ H
Sbjct: 287 KGGGHAWPGAVIPIINGGKSSQNMDASRTVVEFFNAH 323


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.320    0.132    0.393 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,051,129
Number of extensions: 38411
Number of successful extensions: 161
Number of sequences better than 1.0e-08: 1
Number of HSP's gapped: 159
Number of HSP's successfully gapped: 1
Length of query: 356
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 270
Effective length of database: 1,212,660
Effective search space: 327418200
Effective search space used: 327418200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 131 (55.1 bits)