BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0143.p (365 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312487|ref|YP_003776579.1| multidrug resistance transport ... 138 8e-34 gi|300312565|ref|YP_003776657.1| multidrug resistance transmembr... 127 2e-30 gi|300312016|ref|YP_003776108.1| multidrug resistance A (translo... 90 2e-19 gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pum... 84 1e-17 >gi|300312487|ref|YP_003776579.1| multidrug resistance transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 397 Score = 138 bits (347), Expect = 8e-34, Method: Compositional matrix adjust. Identities = 112/287 (39%), Positives = 166/287 (57%), Gaps = 2/287 (0%) Query: 52 LQHTDNAYVRGRTAVIAPQVSGYVVEVPVKDYQQVQRGQVLVRIDSRIYAARVEQAKAAV 111 L+ T++A+V+ + ++ +VSGYV +V V D Q V+ GQ LVRID+R Y+A+ QAKA + Sbjct: 66 LESTNDAFVQADSVTVSSKVSGYVDQVLVADNQIVKAGQPLVRIDARDYSAQAAQAKAQI 125 Query: 112 AAQIAQLENATQAKRARAAAITAQDAALANAQAQLQRARMDLARVDELVSDGSVALRERD 171 A A E A + AAI A LA A + A ++ R L + G+ RER Sbjct: 126 DAAAASEEAANAQLAEQQAAIAQAKAQLAAADEDARFATAEVERYTPLAASGA-ETRERL 184 Query: 172 QNL-AAHAQAEAQVRQASAALDMARQDLRTVEVGRDALVANVEAAHAQLRLAQIDLDNTV 230 L AQA + V AALD A+ +R+++ A E A AQ A ++L T Sbjct: 185 TTLHNQQAQARSTVAARRAALDAAQLRVRSLQAQVRQAQAQGETAKAQYDAASVNLGYTE 244 Query: 231 IRAAEDGQLGEVGVRLGQYVTNGTQLLQLVPAERWVVAHFKEAQTAAMRPGQYAEVRIDA 290 IRA+ DG++G+ VR+GQ+V+ GT+++ +VP +V A+FKE Q MR GQ A +++DA Sbjct: 245 IRASVDGRIGDKQVRVGQFVSPGTRMMTVVPTRFYVSANFKETQIGIMRIGQPAIIKVDA 304 Query: 291 LEGQRLSGRVQLLAPAAGSEFAAIKPDTTTGNFVKIPQRIGVKIVFD 337 L G G V+ L+P G++F+ + P TGNF KI QR+ V++ D Sbjct: 305 LPGVEFHGHVESLSPGTGAQFSLLPPQNATGNFTKIVQRVPVRVAID 351 >gi|300312565|ref|YP_003776657.1| multidrug resistance transmembrane efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 411 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 110/319 (34%), Positives = 157/319 (49%), Gaps = 30/319 (9%) Query: 43 WHLPPFDGPLQHTDNAYVRGRTAVIAPQVSGYVVEVPVKDYQQVQRGQVLVRIDSRIYAA 102 W+L TDNAY A + P ++G V V V D Q V++G VLVRID Sbjct: 54 WYL--IGSRYVETDNAYTATEIATVTPAINGIVAAVDVVDTQAVKKGDVLVRIDDADARL 111 Query: 103 RVEQAKAAVAAQIAQLENATQAKRARAAAITAQ-----------DAALANAQAQLQRARM 151 V+QA AA + + E + A A + AQ A L +AQA L+RA + Sbjct: 112 AVDQA----AADLDRTERKVKGFFANDAGLAAQVLAREAEQKRASAQLLSAQADLKRAEI 167 Query: 152 DLARVDELVSDGSVALRERDQNLAAHAQAEAQVRQASAALDMARQDLRTVEVGRDA---L 208 DL R + L GSV+ E A A+A ++ A AA +R +++ + + A L Sbjct: 168 DLQRREALAKSGSVSGEELSNARTALLTAQANLKAAEAAEVQSRANIKATQGAQKASTVL 227 Query: 209 VAN--------VEAAHAQLRLAQIDLDNTVIRAAEDGQLGEVGVRLGQYVTNGTQLLQLV 260 AN V A A+L A++DL+ TV+RA DG + V++GQ V +G LL +V Sbjct: 228 TANTTVDDNPEVVLARAKLEQAKLDLERTVLRAPVDGVIARRQVQVGQRVQSGATLLSVV 287 Query: 261 PAERWVV-AHFKEAQTAAMRPGQYAEVRIDALEGQ-RLSGRVQLLAPAAGSEFAAIKPDT 318 P ++ V A+FKE Q +R GQ ++ D G G V L+ GS FA I Sbjct: 288 PLQQMHVDANFKEGQLTKVRIGQPVTMKADLYGGSVEYHGVVTGLSGGTGSAFAVIPAQN 347 Query: 319 TTGNFVKIPQRIGVKIVFD 337 TGN++K+ QR+ V+I D Sbjct: 348 ATGNWIKVVQRLPVRISLD 366 >gi|300312016|ref|YP_003776108.1| multidrug resistance A (translocase) transmembrane secretion protein [Herbaspirillum seropedicae SmR1] Length = 406 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 100/310 (32%), Positives = 151/310 (48%), Gaps = 17/310 (5%) Query: 52 LQHTDNAYVRGRTAVIAPQVSGYVVEVPVKDYQQVQRGQVLVRIDSRIYAARVEQAKAAV 111 + TD+AYV G I+ QV G VV V D Q V+ GQ LV +D+ ++QA+AA+ Sbjct: 49 FEETDDAYVGGNVVQISAQVGGTVVAVKADDTQVVKAGQQLVALDAADTRLALDQAQAAL 108 Query: 112 AAQIAQLENATQAKRARAAAITAQDAALANAQAQLQRARMDLARVDELVSDGSVALRE-- 169 A + Q AA + A D+ LA RAR DL R +S G+V+ + Sbjct: 109 AQAVRQTRQLFLNNDTLAANVAAADSNLA-------RAREDLQRRQAGLSSGAVSQEDVS 161 Query: 170 RDQNLAAHAQAEAQVRQASAALDMARQDLRTVEVGRDALVANVEAAHAQLRLAQIDLDNT 229 ++ A A +A+AA + A D +V + L A +A L A+++ Sbjct: 162 HARDAVKSAVAALDQARAAAAANRALTDHTSVTEHPNVLQAATAVRNAYLNYARVN---- 217 Query: 230 VIRAAEDGQLGEVGVRLGQYVTNGTQLLQLVPAER-WVVAHFKEAQTAAMRPGQYAEVRI 288 I A G + + V++GQ + G L+ +VP E+ W+ A+FKE+Q +R GQ EV Sbjct: 218 -IVAPVSGFVSKRSVQVGQRIAAGNPLMAIVPLEQIWIDANFKESQLQHIRIGQPVEVIA 276 Query: 289 DALEGQ-RLSGRVQLLAPAAGSEFAAIKPDTTTGNFVKIPQRIGVKIVFD-DAASAALLR 346 D + G V + G F+ + TGN++K+ QR+ V+I D + A LR Sbjct: 277 DVYGSSVKYKGTVIGFSAGTGGAFSLLPAQNATGNWIKVVQRVPVRIALDPEQVRAHPLR 336 Query: 347 PGMSVEATVD 356 G+S ATVD Sbjct: 337 IGLSTTATVD 346 >gi|300311421|ref|YP_003775513.1| multidrug resistance efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 324 Score = 84.3 bits (207), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 101/351 (28%), Positives = 160/351 (45%), Gaps = 54/351 (15%) Query: 21 SRLRLLLALAVLAAAVLLILHAW----HLPPFDGPLQHTDNAYVRGRTAVIAPQVSGYVV 76 S R+L+ A+ A+LL W H P T + VR IAP VSG VV Sbjct: 5 SIFRVLITTAIFLLAILLAWSLWQHYMHSP-------WTRDGRVRAEVVNIAPDVSGQVV 57 Query: 77 EVPVKDYQQVQRGQVLVRIDSRIYAARVEQAKAAVAAQIAQLENATQAKRARAAAITAQD 136 +PV+D Q V++G +L+ ID Y ++QA+AAV A+ A+L+ R A + A+ Sbjct: 58 ALPVRDNQLVKKGDLLMEIDPARYTLALQQAEAAVTARKAELD-------VRRAEVEARR 110 Query: 137 AALANAQAQLQRARMDLARVDELVSDGSVALRERDQNLAAHAQAEAQVRQASAALDMARQ 196 A L N + R R D+ D V RE A A +QV + A L+ A+ Sbjct: 111 ADL-NMRRAQARRRADV--------DALVVSRE------AREDAGSQVTASQAQLEAAQA 155 Query: 197 DLRTVEVGRDALVANVEAAHAQLRLAQIDLDNTVIRAAEDGQLGEVGVRLGQYVTNGTQL 256 L+ + A ++ + A +++L+ T + + DG + + VR G Y G Sbjct: 156 QLQGAQAQYQAALSQRDTA-------RLNLERTKVFSPVDGYITNLNVRRGDYAAAGAAK 208 Query: 257 LQLVPAER-WVVAHFKEAQTAAMRPGQYAEVRIDALEGQRLSGRVQLLA--------PAA 307 + ++ + WV A+F+E + + G A RI + G + G V +A P + Sbjct: 209 IAVIDSHSFWVYAYFEETKLPLLSIGDRA--RIQLINGASMEGHVDSIARGIYDRDNPQS 266 Query: 308 GSEFAAIKPDTTTGNFVKIPQRIGVKIVFDDAASAALLRPGMSVEATVDPG 358 A + P T N+V++ QRI V+I D LL G++ ++PG Sbjct: 267 RELTADVNP---TFNWVRLAQRIPVRIQLDKVPENVLLASGLTCTVVLEPG 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.129 0.354 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 929,194 Number of extensions: 34694 Number of successful extensions: 224 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 211 Number of HSP's successfully gapped: 7 Length of query: 365 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 279 Effective length of database: 1,212,660 Effective search space: 338332140 Effective search space used: 338332140 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)