BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0142.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309656|ref|YP_003773748.1| outer membrane drug efflux prot... 94 8e-21 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 90 2e-19 gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprot... 90 2e-19 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 86 2e-18 gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipo... 84 1e-17 >gi|300309656|ref|YP_003773748.1| outer membrane drug efflux protein [Herbaspirillum seropedicae SmR1] Length = 504 Score = 94.4 bits (233), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 83/257 (32%), Positives = 128/257 (49%), Gaps = 33/257 (12%) Query: 32 EQAAVTLPAQWREPLASTQ---------------GISLSRWWEAFADPVLNQLVDQVLAG 76 E+ +V PA ++E +TQ I+ WW+ F D LN L D Sbjct: 38 ERPSVDTPAAFKEAAPATQTGENGSQWKTAQPAEDIARGEWWKIFGDEKLNALEDTAQQA 97 Query: 77 NSDLAMAAARVQQARASSRLATAQRMPSLSASGMGA--------HQGDVDAFGHARVQDA 128 N DL AAAR+ QARA +R A A P + A G+GA QG + A R Sbjct: 98 NQDLKAAAARLGQARALTRDARAGYFPQVDA-GVGATRQRPSPASQG-LPADADTRPNTL 155 Query: 129 WQAELSLSYELDLFGRLADQDAAARASLLATDAARSTLRLALIATATSTYIALRAQQARL 188 ++A++++SYE+DLFGR+A AA A ++A ++ LAL A Y +R A Sbjct: 156 YRAQVNVSYEVDLFGRVASTVDAATADAQRSEALFHSVLLALQADVAQQYFLVRELDAAA 215 Query: 189 EVLDQTLEMRAQSLQLARRRAQQGYASRLEVEQSQSEYAATEQLIASTRMSISRN----E 244 + T+++R +SL+L +RR +G S ++V ++++E A+ + S + I+R E Sbjct: 216 ALYRGTVDLRQESLRLVQRRFDEGDISEVDVARARAELASAQ----SEALGIARQRATAE 271 Query: 245 HALALLAXRLHSHEHQP 261 HALA+L R + P Sbjct: 272 HALAILLGRTPAEFSLP 288 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 75/250 (30%), Positives = 132/250 (52%), Gaps = 16/250 (6%) Query: 14 VTLAAMLAACA-GP---RPGQPEQAAVTLPAQWREPLASTQGISLSRWWEAFADPVLNQL 69 + +A +L C+ P RP P +++ A R A Q ++L W + F D L QL Sbjct: 19 LVMAGVLGGCSLAPTYERPQAPVESSYPADAAGR---AELQAVNLG-WRQFFPDQRLQQL 74 Query: 70 VDQVLAGNSDLAMAAARVQQARASSRLATAQRMPSLSASGMGAHQ---GDVDAFGHARVQ 126 + L N DL A +++ARA ++A A +P+L+ SG A G + Sbjct: 75 IAAALENNRDLRTAVLNIEEARAQYQIARADLLPNLNLSGNAARTRTAASQSLTGQPTIS 134 Query: 127 DAWQAELSL-SYELDLFGRLADQDAAARASLLATDAARSTLRLALIATATSTYIALRAQQ 185 +++Q L++ +YELD FGR+ + AA AS L+T+ A+ + +++L+A Y+A R+ Sbjct: 135 NSYQVGLAVPAYELDFFGRVRNLKDAALASYLSTEEAQKSAQISLVAQVAQAYLAERSYA 194 Query: 186 ARLEVLDQTLEMRAQSLQLARRRAQQGYASRLEVEQSQSEYAATEQLIASTRMSISR--N 243 +L++ D+TL+ R +L LA++R G ++ L+ +Q+ + +A T++ R Sbjct: 195 EQLKLADETLKSRQSNLDLAQKRLDVGASTALDFRLTQTLVESAR--VAKTQLERQRAQA 252 Query: 244 EHALALLAXR 253 E+AL LL + Sbjct: 253 ENALTLLVGK 262 >gi|300311796|ref|YP_003775888.1| outer membrane channel lipoprotein [Herbaspirillum seropedicae SmR1] Length = 510 Score = 90.1 bits (222), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 86/259 (33%), Positives = 146/259 (56%), Gaps = 16/259 (6%) Query: 4 PSRSAQIISLVTLAAMLAACA-GPRPGQPEQAAVTLPAQWRE----PLASTQGISLSR-W 57 P R+ + ++ + LAA+L CA GP +P A+ LP ++E LA+ + I + W Sbjct: 28 PFRATKGMTALVLAALLGGCAVGPDYARP---ALDLPQAYKETGPWKLAAPRQIDDHQPW 84 Query: 58 WEAFADPVLNQLVDQVLAGNSDLAMAAARVQQARASSRLATAQRMPSLSASGMGAHQGDV 117 WEA+ DPVLN L+DQ A N + AAA+ +QA+A++ +A A P++ A GA + Sbjct: 85 WEAYGDPVLNALMDQANAANQTIQQAAAQYRQAQATAEVARASLWPTIGAQA-GASRAQT 143 Query: 118 DAFGHARVQDAWQAELSLSYELDLFGRLADQDAAARASLLATDAARSTLRLALIATATST 177 + G R+ +++ L+ S+E DL+GR+ A A+ A+ A+ + RL++ AT Sbjct: 144 NTNGVQRLGNSYSVGLNASWEADLWGRIRRGAEAGEANAQASAASLAAARLSIQATLAQD 203 Query: 178 YIALRAQQARLEVLDQTLEMRAQSLQLARRRAQQGYASRLEVEQSQSEY-AATEQLIAST 236 Y+ LR + ++ +T+ +SLQL+ + + G A R +V Q++++ AA QLI Sbjct: 204 YLQLRVTDLQKDLYRRTVAAYTRSLQLSTHQYEAGTALRSDVAQAETQLRAAQAQLI--- 260 Query: 237 RMSISRN--EHALALLAXR 253 + +RN EHA+A+L + Sbjct: 261 DLDDTRNQLEHAIAMLIGK 279 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 86.3 bits (212), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 62/201 (30%), Positives = 102/201 (50%), Gaps = 4/201 (1%) Query: 57 WWEAFADPVLNQLVDQVLAGNSDLAMAAARVQQARASSRLATAQRMPSLSASGMGAHQ-- 114 W + F DP L Q++ L N DL +A +++ARA + A PS+ A+ Sbjct: 66 WRDVFTDPSLQQVIALALENNRDLRVAVLNIEKARAQYGVQRAALFPSIKAATSETASRT 125 Query: 115 -GDVDAFGHARVQDAWQAELSLS-YELDLFGRLADQDAAARASLLATDAARSTLRLALIA 172 GD+ + G A ++ A L S YELDLFGR+ A S L+T AR + +++LIA Sbjct: 126 PGDLSSTGSALTSRSYTATLGFSAYELDLFGRVRSLKEQALQSFLSTAEARRSTQISLIA 185 Query: 173 TATSTYIALRAQQARLEVLDQTLEMRAQSLQLARRRAQQGYASRLEVEQSQSEYAATEQL 232 + ++ L + Q RL + TLE ++ S L +R + G +S L + Q+Q+ + Sbjct: 186 EVATAWLTLASDQDRLRLAQDTLESQSSSYALTQRSFELGSSSALTLRQAQTSVESARVD 245 Query: 233 IASTRMSISRNEHALALLAXR 253 + S + ++ +AL LL + Sbjct: 246 VESYTAQVDQDRNALVLLVGK 266 >gi|300311360|ref|YP_003775452.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 470 Score = 83.6 bits (205), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 70/216 (32%), Positives = 110/216 (50%), Gaps = 11/216 (5%) Query: 39 PAQWRE----PLASTQGISLSRWWEAFADPVLNQLVDQVLAGNSDLAMAAARVQQARASS 94 PA W++ P A Q RWW+ F D L L+D+ L N+DLA+AA ++++A+A + Sbjct: 42 PASWQQRGSQPEADVQ-----RWWQGFGDATLTALIDEALRKNNDLALAAIKLRRAQAQA 96 Query: 95 RLATAQRMPSLSASGMGAHQGDVDAFGHARVQDAWQAELSLSYELDLFGRLADQDAAARA 154 LA +P+LSAS + +D G A Q+ + LSYE+DL+GRLA +R Sbjct: 97 GLADDAFVPALSASLGASSSKQLD--GDRISTRASQSSVKLSYEVDLWGRLAAASDLSRW 154 Query: 155 SLLATDAARSTLRLALIATATSTYIALRAQQARLEVLDQTLEMRAQSLQLARRRAQQGYA 214 LAT+ R + L LI T + Y R+ +Q++ ++L+L R + Q G Sbjct: 155 EALATEQDRQSTALTLIGTTANLYWTGAYLNQRVATAEQSIAYAEKTLELVRTQYQAGAV 214 Query: 215 SRLEVEQSQSEYAATEQLIASTRMSISRNEHALALL 250 S +EV +Q + + N++ALA+L Sbjct: 215 SSVEVLSAQQNLYSQQSSYTELVQQRVENDNALAIL 250 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.123 0.339 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 571,002 Number of extensions: 17053 Number of successful extensions: 131 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 17 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)