BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0141.p (230 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipo... 113 1e-26 gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbas... 112 4e-26 gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipo... 108 5e-25 gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipo... 104 6e-24 gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspiri... 102 3e-23 >gi|300311155|ref|YP_003775247.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 491 Score = 113 bits (283), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 86/201 (42%), Positives = 110/201 (54%), Gaps = 8/201 (3%) Query: 14 AGLQALTLPAFRPDQPAALLRQRPDIFQAEQQLVAADHTLDAARAALMPSLRIGLSGGVV 73 AGL A+ P +P PA LL +RPD+ AE +L+AA+ + AARAA PS I L+G V Sbjct: 277 AGLDAVFAP-LQPGLPALLLTRRPDVAAAEHRLMAANANIGAARAAFFPS--ISLTGNVG 333 Query: 74 G-----SNLLPDPLSIFSAGGSILAPLLDGGRLRAQADATAARRDEAAYAYQKAVLVAWR 128 S L D ++ SI P+ GRLR + RRD A AY+K V A+R Sbjct: 334 TASAELSGLFRDGSHAWTFSPSITLPIFTAGRLRNNLALSEVRRDLAVAAYEKTVQTAFR 393 Query: 129 DVEDALAAARGAQAQQQAVQEQEVALREALAMSRKRYRAGYSPYLEQLDAQRGLLSAQLL 188 +V DALAA + Q + Q RE +S RY G +P+L+ LDAQR LLSAQ Sbjct: 394 EVADALAARQWLTQQLEIAQSAVRVQRERARLSALRYDNGAAPFLDVLDAQRDLLSAQQQ 453 Query: 189 EVQARADRLSAMVSLAQALGG 209 VQ R LS+ V+L ALGG Sbjct: 454 LVQIRRALLSSSVALYAALGG 474 >gi|300309818|ref|YP_003773910.1| drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 483 Score = 112 bits (279), Expect = 4e-26, Method: Compositional matrix adjust. Identities = 77/188 (40%), Positives = 108/188 (57%), Gaps = 9/188 (4%) Query: 29 PAALLRQRPDIFQAEQQLVAADHTLDAARAALMPSLRIGLSGGVVGSN-----LLPDPLS 83 P+ LL RPDI AEQ L+AA+ + AARAA P RI L+G ++ L Sbjct: 287 PSDLLTNRPDIRSAEQSLLAANANIGAARAAFFP--RISLTGNFGSASSTLGGLFESGSG 344 Query: 84 IFSAGGSILAPLLDGGRLRAQADATAARRDEAAYAYQKAVLVAWRDVEDALAAARGAQAQ 143 +S G S+ P+ D GR A D R+++A Y+KA+ A+R+V DAL A RG+ + Sbjct: 345 AWSFGPSLSLPIFDFGRNSANLDLAEVRKNKAVVTYEKAIQTAFREVADALVA-RGSLDE 403 Query: 144 QQAVQEQEVALR-EALAMSRKRYRAGYSPYLEQLDAQRGLLSAQLLEVQARADRLSAMVS 202 Q QE +A + E L ++ R++ G + L+QLDAQR L SAQ +QAR RL+ + Sbjct: 404 QVKAQEAFLAAQQERLKLTDLRFKNGIASSLDQLDAQRDLFSAQQALIQARQLRLNNAID 463 Query: 203 LAQALGGG 210 L +ALGGG Sbjct: 464 LYKALGGG 471 >gi|300310691|ref|YP_003774783.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 498 Score = 108 bits (269), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 80/216 (37%), Positives = 113/216 (52%), Gaps = 25/216 (11%) Query: 20 TLPAFRPDQPAALLRQRPDIFQAEQQLVAADHTLDAARAALMPSLRIGLSGGVVGSNLLP 79 T+P P P+ LL++R DI QAE+ L AA+ + AARAA PS+ + ++ G + L Sbjct: 290 TIP---PGLPSDLLQRRADIVQAERDLRAANANIGAARAAFYPSITLTVNAGTASARLA- 345 Query: 80 DPLSIFSAG-GS------ILAPLLDGGRLRAQADATAARRDEAAYAYQKAVLVAWRDVED 132 +F G GS I P+ DGG +A D RD + Y+KA+ A+R+V D Sbjct: 346 ---GLFKGGSGSWSFVPQISLPIFDGGANQANLDIATVSRDISVAQYEKAIQTAFREVSD 402 Query: 133 ALAA----ARGAQAQQQAVQEQEVALREALAMSRKRYRAGYSPYLEQLDAQRGLLSAQLL 188 ALA R QAQ+ VQ + EA +S+ RY +G YL LD+QR L SAQ Sbjct: 403 ALAQRNTLGRQLQAQEALVQASD----EAFRLSQARYHSGVDSYLTVLDSQRTLYSAQKN 458 Query: 189 EVQARADRLSAMVSLAQALGGGWEAERGQDLSPQAS 224 + + R + +V+ + LGGGW G +P AS Sbjct: 459 LIGTQLSRWTNLVTFYKTLGGGWVERTG---TPAAS 491 >gi|300313892|ref|YP_003777984.1| outer membrane drug efflux lipoprotein [Herbaspirillum seropedicae SmR1] Length = 497 Score = 104 bits (259), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 82/220 (37%), Positives = 110/220 (50%), Gaps = 16/220 (7%) Query: 12 LSAGL--QALTLPAFRPDQPAALLRQRPDIFQAEQQLVAADHTLDAARAALMPSLRIGLS 69 L AGL A LPA P+ +L +RPD+ AE L +A+ + AARAA PS+ + + Sbjct: 280 LPAGLPDTASVLPATPAGLPSQVLLRRPDVLAAEHTLKSANADIGAARAAFFPSISLTAN 339 Query: 70 GGVVGSNLLPDPLSIFSAGG-------SILAPLLDGGRLRAQADATAARRDEAAYAYQKA 122 GG L +F AG SI P+ + G LRA D+ RD Y+KA Sbjct: 340 GGTASRTLG----GLFKAGSGSWTFSPSINLPIFNAGSLRASLDSAKITRDIDVANYEKA 395 Query: 123 VLVAWRDVEDALAAARGAQAQQQAVQEQEVALRE-ALAMSRKRYRAGYSPYLEQLDAQRG 181 + A+R+V DALA R Q+ Q + A E A +S RYR G YL L AQR Sbjct: 396 IQTAFREVADALAV-RATLDQRLDAQGKATAASEKAFRLSDARYRNGVDSYLTTLVAQRT 454 Query: 182 LLSAQLLEVQARADRLSAMVSLAQALGGGWEAERGQDLSP 221 L SAQ + R S ++L + LGGGW +E+ Q + P Sbjct: 455 LYSAQQTLISLRLTEQSNRITLYKVLGGGW-SEQTQPMQP 493 >gi|300310854|ref|YP_003774946.1| NodT family protein [Herbaspirillum seropedicae SmR1] Length = 507 Score = 102 bits (253), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 78/228 (34%), Positives = 116/228 (50%), Gaps = 16/228 (7%) Query: 7 EHALALSAGLQ-----------ALTLPAFRPDQPAALLRQRPDIFQAEQQLVAADHTLDA 55 EHA+AL G A P P+ LL +RPD+ AE+++ +A+ + Sbjct: 260 EHAIALLVGKTPAQFALPRAPLAAVAPPVPQGVPSMLLERRPDVAAAERRVASANAKIGV 319 Query: 56 ARAALMPSLRIGLSGGVVGSNL---LPDPLSIFSAGGSILAPLLDGGRLRAQADATAARR 112 A+AA PSL + SGG S P ++S G + + DGG A D A Sbjct: 320 AKAAYFPSLTLSASGGYQSSTFADWFTLPGRVWSLGPQLAQTIFDGGARAAATDQAIATY 379 Query: 113 DEAAYAYQKAVLVAWRDVEDALAAARGAQAQQQAVQEQEV-ALREALAMSRKRYRAGYSP 171 D Y++ VL A+++VED LAA R + Q+ AVQ + + A R+A+ + +Y+AG Sbjct: 380 DGTVATYKQTVLTAFQEVEDNLAALRILE-QEAAVQGEALQAARQAVTLVNNQYKAGTVS 438 Query: 172 YLEQLDAQRGLLSAQLLEVQARADRLSAMVSLAQALGGGWEAERGQDL 219 Y+ + AQ LSA+L + R++A V LA+ALGGGW+ DL Sbjct: 439 YVNVITAQATALSAELSSLNILNRRMAASVLLAKALGGGWDERSLDDL 486 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.129 0.353 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 503,394 Number of extensions: 13516 Number of successful extensions: 121 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 108 Number of HSP's successfully gapped: 17 Length of query: 230 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 148 Effective length of database: 1,231,600 Effective search space: 182276800 Effective search space used: 182276800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)