BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0131.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 359 e-100 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 117 8e-28 gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase o... 117 1e-27 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 107 1e-24 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 102 4e-23 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 359 bits (921), Expect = e-100, Method: Compositional matrix adjust. Identities = 172/262 (65%), Positives = 215/262 (82%), Gaps = 2/262 (0%) Query: 1 MRLDNKVAIVTGAASGIGKEIALTFARAGAKVAIADLNLQAAVATAVEIEAIAGKGSAIG 60 M L +KVA++TG+ASGIGKEIA+ +AR GAKV IADL L+AA ATA EI + G+A+ Sbjct: 1 MLLKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEI--VKEGGTAMA 58 Query: 61 VAMDVSNEEQVNTGIDNVVSHFGRLDVLVSNAGIQIVAPLDQFDFAKWKQMLAIHLDGAF 120 VAM+V++E QV+ G+ + V+ +G LD+++SNAGIQI++P+ + W++MLAIHLDGAF Sbjct: 59 VAMNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAF 118 Query: 121 LTTKAALRHMYELGNGGSIIYMGSVHSKEASLLKAPYVTAKHGLIGLAKAVAKEGAKHGV 180 LTT+AA++ M + G GGSIIYMGSVHS AS LKAPYVTAKHGL+GLAK VAKEGAK + Sbjct: 119 LTTRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKI 178 Query: 181 RANVVCPGFVRTPLVEKQIPEQARTLGISEQDVVKNVMLKETVDGEFTTVQDVAEATLFF 240 R NV+CPGFVRTPLVEKQIPEQA+ GISE+DV+K +MLK+TVDGEFTT QDVA+ +F Sbjct: 179 RTNVICPGFVRTPLVEKQIPEQAKEFGISEEDVIKKIMLKDTVDGEFTTTQDVAQTAVFL 238 Query: 241 AGFGSNALTGQSLVVSHGWFMQ 262 A F +NALTGQS+VVSHGW MQ Sbjct: 239 AAFPTNALTGQSVVVSHGWHMQ 260 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 117 bits (294), Expect = 8e-28, Method: Compositional matrix adjust. Identities = 81/258 (31%), Positives = 137/258 (53%), Gaps = 8/258 (3%) Query: 1 MRLDNKVAIVTGAASGIGKEIALTFARAGAKVAIADLNLQAAVATAVEIEAIAGKGSAIG 60 + L ++ IVT A GIG I F AGA V I D++ + +A+A A G Sbjct: 3 LNLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVS-EDFLASA---RARFGHAPVSY 58 Query: 61 VAMDVSNEEQVNTGIDNVVSHF-GRLDVLVSNAGIQ-IVAPLDQFDFAKWKQMLAIHLDG 118 DVS+E +V+ ++ + GRLDVL++NAGI +P+++ + W Q LA++L G Sbjct: 59 SRTDVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTG 118 Query: 119 AFLTTKAALRHMYELGNGGSIIYMGSVHSKEASLLKAPYVTAKHGLIGLAKAVAKEGAKH 178 FL T+ A+ + + G GG+I+ + SV + L+ PY +K+G+IGL + A E Sbjct: 119 PFLCTRRAVPLLKKNG-GGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPS 177 Query: 179 GVRANVVCPGFVRTPLVEKQIPEQARTLGISEQDVVKNVMLKETVDGEFTTVQDVAEATL 238 +R N V PG V E+ + +A GI +++ + ++ + ++ + T +D+A + Sbjct: 178 HIRVNAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLR-KMVTAEDIANQVI 236 Query: 239 FFAGFGSNALTGQSLVVS 256 F +++GQSL V Sbjct: 237 FLCSPAGASISGQSLSVC 254 >gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 708 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 98/264 (37%), Positives = 139/264 (52%), Gaps = 14/264 (5%) Query: 3 LDNKVAIVTGAASGIGKEIALTFARAGAKVAIADLNLQAAVATAVEIEAIAGKGSAIGVA 62 L ++A+VTG GIG+ +A GA V + D++ Q+A+ +A + G AIGV Sbjct: 444 LAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDID-QSALDSAQQSLLKHGGPDAIGVV 502 Query: 63 M-DVSNEEQVNTGIDNVVSHFGRLDVLVSNAGIQIVAPLDQFDFAKWKQMLAIHLDGAFL 121 D+++E V T +++V FG +D+LVSNAGI +PL W++ L++ G FL Sbjct: 503 RADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFL 562 Query: 122 TTKAALRHMYELGNGGSIIYMGSVHSKEASLLKAPYVTAKHGLIGLAKAVAKEGAKHGVR 181 ++AA M G GGSI+Y+ S + AS + Y TAK I LA+ +A EGA HG+R Sbjct: 563 VSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIR 622 Query: 182 ANVVCP-GFVRTPLVE--KQIPEQARTLGISEQDV----VKNVMLKETVDGEFTTVQDVA 234 NVV P +R + K E+A + I DV + MLK +V E D+A Sbjct: 623 VNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPE-----DIA 677 Query: 235 EATLFFAGFGSNALTGQSLVVSHG 258 EA FFA S TG L V G Sbjct: 678 EAVYFFASEKSAKSTGNILNVDAG 701 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 76/254 (29%), Positives = 123/254 (48%), Gaps = 14/254 (5%) Query: 6 KVAIVTGAASGIGKEIALTFARAGAKVAIADLNLQAAVATAVEIEAIAGKGSAIGVAMDV 65 +VA+VTG A GIG I+ A G V +AD+NL AA TA + +A A + MD+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADAL--VAAGHRAAPLQMDL 66 Query: 66 SNEEQVNTGIDNVVSHFGRLDVLVSNAGIQIVAPLDQFDFAKWKQMLAIHLDGAFLTTKA 125 + E + ++ +GR DVLV+NAG+ + W Q + +++ G L + Sbjct: 67 GSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQH 126 Query: 126 ALRHMYELGNGGSIIYMGSVHSKEASLLKAPYVTAKHGLIGLAKAVAKEGAKHGVRANVV 185 A R M + G I+ + S+ A + Y T+K +IGL + +A E A++G+ N V Sbjct: 127 AARLMVKQ-QWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSV 185 Query: 186 CPGFVRTPLVEKQIPEQARTLGISEQDVVKNVMLKETVDGEFTTVQDVAEATLFFAGFGS 245 PG V TP+ + EQ R + + V ++ + T ++A A + A S Sbjct: 186 APGPVDTPMTQAMHSEQTR------ESYYRLVPMR-----RYGTTDEIASAVCYLAAEES 234 Query: 246 NALTGQSLVVSHGW 259 +TG + V G+ Sbjct: 235 AYITGHVIPVDGGF 248 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 102 bits (254), Expect = 4e-23, Method: Compositional matrix adjust. Identities = 79/267 (29%), Positives = 129/267 (48%), Gaps = 23/267 (8%) Query: 1 MRLDNKVAIVTGAA--SGIGKEIALTFARAGAKVAIADLNLQAAVATAVEIEAIAGKGSA 58 M L +KVA++TG A +G+G A A GA+V + DL A A I + Sbjct: 1 MLLKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGS-----QH 55 Query: 59 IGVAMDVSNEEQVNTGIDNVVSHFGRLDVLVSNAGIQIVAPLDQFDFAKWKQMLAIHLDG 118 +G+ DV+++ T ++ FGR+D+LV+NAGI + A + ++L + L G Sbjct: 56 LGLVADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRG 115 Query: 119 AFLTTKAALRHMYELGNGGSIIYMGSVHSKE-ASLLKAP-YVTAKHGLIGLAKAVAKEGA 176 ++A L M N GSII + SV ++ +L P Y AK G++GLA+A+A+E Sbjct: 116 TLYMSQAVLPAMRAQQN-GSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFG 174 Query: 177 KHGVRANVVCPGFVRTPLVEKQIPEQARTLGISEQDVVKNVMLKETVD-GEFTTVQDVAE 235 G+R N + PG + T +++ ++ E+ K + ET+ D+ Sbjct: 175 PEGIRVNSITPGLIGTDIIKGKLTEE------------KKAEIAETIPLARLGRADDIGG 222 Query: 236 ATLFFAGFGSNALTGQSLVVSHGWFMQ 262 A +F S TG +L V+ G + Sbjct: 223 ACVFLGSDLSQYCTGITLDVNGGMLIH 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 729,759 Number of extensions: 28155 Number of successful extensions: 254 Number of sequences better than 1.0e-08: 45 Number of HSP's gapped: 176 Number of HSP's successfully gapped: 45 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)