BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0126.p (416 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance ... 594 e-171 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 63 4e-11 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 62 8e-11 gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprote... 55 9e-09 >gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 594 bits (1531), Expect = e-171, Method: Compositional matrix adjust. Identities = 303/383 (79%), Positives = 332/383 (86%), Gaps = 13/383 (3%) Query: 46 HREEGGHKDKTAHDDDKDDEHK------EGEH------KEEGKIALSKEQMAAAGIRVDT 93 H+EEGGHKD H ++K EHK EGEH EEGKIAL+ EQ+A AGI V T Sbjct: 15 HQEEGGHKDNEHHGEEKGSEHKDAAEHGEGEHHDAEPSSEEGKIALNPEQIATAGIEVKT 74 Query: 94 AGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQKVKKGQVLAVISSSAVS 153 AGP+ + S+VQLPGEIRFNEDRTAHVVPQVAGIVESVSA LGQKV+KGQVLAVI+SSAVS Sbjct: 75 AGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVRKGQVLAVINSSAVS 134 Query: 154 EQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA 213 EQRS++LNAEQRL+LAR+AYQREK LWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA Sbjct: 135 EQRSEMLNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRA 194 Query: 214 IGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFLISDLSSVWAEIIVSPKD 273 IGAT SSG LNRYEIRAPFDG+VVEKHLGLGEAV+EDANAFLISDLSSVWAEIIVSPKD Sbjct: 195 IGAT-ASSGGLNRYEIRAPFDGVVVEKHLGLGEAVKEDANAFLISDLSSVWAEIIVSPKD 253 Query: 274 IQVVRVGEKVVVRATAFDASAEGKISYVGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNV 333 +Q VR GEKV VRATAFDASA GKISYVGSLIGEQSRTAKAHV+LPN D WRPGLFVNV Sbjct: 254 MQFVRTGEKVTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPNPDDSWRPGLFVNV 313 Query: 334 EVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFVAKAVSLGRSDGKSVEITSGLDAGT 393 EV++ E QVPVAV+A+A+QTVE ++V+FVR GF A+ V GRS+GK VEI SGL+AG Sbjct: 314 EVVSDEAQVPVAVSAEALQTVEEKSVIFVRVPGGFAAQQVVTGRSNGKFVEIRSGLNAGD 373 Query: 394 GYAATGSFAIKAELGKGSAEHSH 416 YAATGSFAIKAELGKGSAEHSH Sbjct: 374 EYAATGSFAIKAELGKGSAEHSH 396 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 73/301 (24%), Positives = 141/301 (46%), Gaps = 27/301 (8%) Query: 102 TVQLPGEIR-FNEDRTAHVVPQVAGIVESVSANLGQKVKKGQVLAVISSSAVSEQRSDLL 160 T+ LPG ++ F + + + + G ++ + ++G +VKKG++LA + + + +Q + L Sbjct: 83 TLALPGTLQGFVQ---SPISARSGGYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQAL 139 Query: 161 NAEQR----LSLARSAYQREKHLWEQKISAEQDYLQAQAALREAEVAVRNAQQKLRAIGA 216 A ++ + LARS+ R + L ++ ++Q+ + ++A +A + A + Sbjct: 140 AAREQAASSMELARSSMARWEALRKKDAVSQQELDERRSAFAQASANLAAADANV----- 194 Query: 217 TGTSSGNLNRYE----IRAPFDGIVVEKHLGLGEAVREDAN--AFLISDLSSVWAEIIVS 270 G L + E I APF GI+ +++ +G+ + A F++S + + V Sbjct: 195 -----GRLRQVEGFKRIVAPFSGIITRRNVDVGDLIDPGAGRALFVLSQTDPLRVYVNVP 249 Query: 271 PKDIQVVRVGEKVVVRATAFDASA-EGKISYVGSLIGEQSRTAKAHVVLPNTGDLWRPGL 329 +V+ G++V V + EGK++ I +R+ + V L N PG Sbjct: 250 QSYANLVKPGQQVTVTQSELRGRKFEGKVARTAGAIDAATRSMQVEVTLSNADHALLPGA 309 Query: 330 FVNVEVIAGETQVPVAVAADAIQTVEGENVVFVRDADGFV-AKAVSLGRSDGKSVEITSG 388 FV V + + + VAAD + +V D V K + LGR+ G++VE+ G Sbjct: 310 FVQVALPIKPSGA-LLVAADTLMFRRDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDG 368 Query: 389 L 389 L Sbjct: 369 L 369 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 62.0 bits (149), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 84/320 (26%), Positives = 140/320 (43%), Gaps = 31/320 (9%) Query: 94 AGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQKVKKGQVLAVISSS--- 150 A P+++ + +LPG R + + A V PQV GI+++ S G VK GQVL I+ + Sbjct: 48 AQPVELHT--ELPG--RTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQ 103 Query: 151 -AVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAALREAEVAVRNAQQ 209 A + L AE + AR R K L K ++QDY A A+L EAE V +A+ Sbjct: 104 AAYDSSVAALAKAEASVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARA 163 Query: 210 KLRAIGATGTSSGNLNRYEIRAPFDGIVVEKHLGLGEAVREDANAFL------------I 257 ++ TS NL+ + AP G + + + G V + L + Sbjct: 164 NVQ------TSRINLDYARLDAPISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDV 217 Query: 258 SDLSSVWAEIIVSPKDIQVVRVGEKVVVRATAFDA---SAEGKISYVGSLIGEQSRTAKA 314 + S+ W + + + Q+ + G VR + + EG + + + + + Sbjct: 218 TQSSTTWLNLKEALANGQLEKSGNGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITL 277 Query: 315 HVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAI-QTVEGENVVFVRDADGFV-AKA 372 + PN PG++V + G Q + V A+ + G+ V FV +A G V +A Sbjct: 278 RAIFPNPKTDLLPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRA 337 Query: 373 VSLGRSDGKSVEITSGLDAG 392 + R+ G ++SGL G Sbjct: 338 LVTDRAIGDQWLVSSGLKEG 357 >gi|300309820|ref|YP_003773912.1| acriflavin resistance lipoprotein A [Herbaspirillum seropedicae SmR1] Length = 402 Score = 55.1 bits (131), Expect = 9e-09, Method: Compositional matrix adjust. Identities = 87/334 (26%), Positives = 141/334 (42%), Gaps = 37/334 (11%) Query: 84 MAAAGIRVDTAGPLQIRSTVQLPGEIRFNEDRTAHVVPQVAGIVESVSANLGQKVKKGQV 143 M + + V T P ++ T +LPG R R A V +VAGIV+ G +VK+GQV Sbjct: 34 MPPSEVSVVTIAPQRVAITNELPG--RVEAFRVAQVRARVAGIVQKRVFKEGSEVKEGQV 91 Query: 144 LAVISS----SAVSEQRSDLLNAEQRLSLARSAYQREKHLWEQKISAEQDYLQAQAALRE 199 L I +++ ++ L A+ L+ QR K L E ++Q+Y A AA ++ Sbjct: 92 LFKIDPAQYKASLESAQATLARAQANLTQTTLTAQRYKPLVEVNAVSKQEYDNAVAAQKQ 151 Query: 200 AEVAVRNAQQKLRAIGATGTSSGNLNRYEIRAPFDG-----IVVEKHL-GLGEAVREDAN 253 AE V + A T+S NL + +P G +V E L G G+A + Sbjct: 152 AEADVAAGK------AAVQTASLNLGYATVTSPISGRIGRALVTEGALVGQGDATQ---- 201 Query: 254 AFLISDLSSVWAEIIVSPKDI----------QVVRVGE-KVVVRATAFDASA---EGKIS 299 L+ L V+ + S D+ Q+ VG+ K V + A GK+ Sbjct: 202 LALVQQLDPVYVNVTQSSADLLKLQQAMAAGQLKSVGDNKASVTLELENGQAYPLPGKLL 261 Query: 300 YVGSLIGEQSRTAKAHVVLPNTGDLWRPGLFVNVEVIAGETQVPVAVAADAIQTVEGENV 359 + + E + + PN L PG +V ++ + + V A+ G + Sbjct: 262 FSDISVDENTGSVSLRAEFPNPKRLLLPGTYVRAKIEQAVDEQALLVPQQAVVRDAGGST 321 Query: 360 VFVRDADGFV-AKAVSLGRSDGKSVEITSGLDAG 392 V V +A+ V + V G + G + ++ GL G Sbjct: 322 VMVVNAENKVEPRPVKTGGARGNNWQVLDGLKEG 355 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.128 0.346 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,066,690 Number of extensions: 39550 Number of successful extensions: 183 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 8 Length of query: 416 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 328 Effective length of database: 1,203,190 Effective search space: 394646320 Effective search space used: 394646320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)