BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0123.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [H... 339 1e-94 gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [H... 107 2e-24 gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [H... 95 8e-21 gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [H... 87 2e-18 >gi|300310813|ref|YP_003774905.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 339 bits (870), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 182/308 (59%), Positives = 227/308 (73%), Gaps = 4/308 (1%) Query: 4 QAIRYHQYGPPDVMALEAIAQFPLEPGQVRVSLRAAGVAPLDAKLRAGLLQAQLALTLPK 63 +AI YHQYGPP V+ L + + PL PG VRV+++AAGVAP+D KLRAG LQ +AL LPK Sbjct: 2 RAICYHQYGPPSVLHLCDLDEQPLPPGHVRVAMQAAGVAPVDTKLRAGRLQHLVALDLPK 61 Query: 64 VPGRDGAGVIVESRAEG---LPVGEPVCVMADMFGQGSYADSIVVPAARVVRQPSTLSTR 120 VPGRDGAGVI + EG L VGE VCVM M G+G+ +V+P +VVR+P LS + Sbjct: 62 VPGRDGAGVIT-ALGEGVHTLQVGEAVCVMPSMAGRGTTLAELVLPQEQVVRKPDVLSMQ 120 Query: 121 QAAALLQPGASAWIPIVRTAQVRSGMKVLVHAGSGAVGSLMIQLAAHLGAEVWATCSSAN 180 QAAALLQP SAWI +++TA + +GMKV +HAGSGAVGSLM+QLAAHLGAEV A+C S+N Sbjct: 121 QAAALLQPACSAWIALMQTAPIAAGMKVFIHAGSGAVGSLMVQLAAHLGAEVTASCRSSN 180 Query: 181 RDDVLALGATGVIAYDKEDFSALRGVDVVFDLVGGDTHDRSYPVLKPGGQLVWLVAAPIR 240 + V ALGA+ V+AYD+++ +R DVV DLVGG HD+SY +L+ GGQLVWLVAAPIR Sbjct: 181 VEYVAALGASRVLAYDRDELGRVRDQDVVIDLVGGAAHDQSYRMLRRGGQLVWLVAAPIR 240 Query: 241 ERGEEFKVRVVRAMIDDAPECLHAVAKLAEERVWVPTVGQCFPLAEAARAHALLESGKAA 300 ERGEEF V V RA+I + L VA LA++ VW P V P+ E ARAH +E+G+ Sbjct: 241 ERGEEFGVSVRRALIVEDQSILQQVAALAQQGVWRPAVSGVLPMQEIARAHQRIEAGEVT 300 Query: 301 RGRIVLDI 308 RGR+VLDI Sbjct: 301 RGRLVLDI 308 >gi|300311093|ref|YP_003775185.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 325 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 102/331 (30%), Positives = 151/331 (45%), Gaps = 33/331 (9%) Query: 4 QAIRYHQYGPPDVMALEAIAQFPLEPGQVRVSLRAAGVAPLDAKLRAGLLQAQLALTLPK 63 +AI ++G P+V+ L+ G+V + + AAGV D R G + Sbjct: 2 RAIEIKEFGGPEVLQACERPVPELKAGEVLIKVHAAGVNRPDVFQRTGNYPVPPGAS--D 59 Query: 64 VPGRDGAGVIVESRA--EGLPVGEPVCVMADMFGQGSYADSIVVPAARVVRQPSTLSTRQ 121 +PG + AG IV G +G+ VC + G YA+ P A+ + P LS + Sbjct: 60 LPGLEVAGEIVAGDLGDSGFKLGDLVCALVQ---GGGYAEFCAAPLAQCLPVPKGLSALE 116 Query: 122 AAALLQPGASAWIPIVRTAQVRSGMKVLVHAGSGAVGSLMIQLAAHLGAEVWATCSSANR 181 AAAL + + W + Q+ +LV GS +G IQ+A LG V+AT SA + Sbjct: 117 AAALPENYFTVWSNVFDRGQLSGEETLLVQGGSSGIGVTAIQIARALGHRVFATAGSAEK 176 Query: 182 DDVL-ALGATGVIAYDKEDFSAL-------RGVDVVFDLVGGDTHDRSYPVLKPGGQLVW 233 LGA I Y EDF A+ RGVDV+ D+VGGD R L G+LV+ Sbjct: 177 CRACEELGAERAINYKTEDFEAVVKELTGGRGVDVILDMVGGDYLPREIKALADDGRLVF 236 Query: 234 LVAAPIRERGEEFKVRVVRAMIDDAPECLH--------AVAKLAEERVW--------VPT 277 +A +G+ +V+R + L A+A E VW P Sbjct: 237 -IAQLGGAKGQLDMGQVMRRRLTITGSTLRPRPVAFKAAIAAKLREHVWPLIEAGKIRPV 295 Query: 278 VGQCFPLAEAARAHALLESGKAARGRIVLDI 308 + FPL +AA+AHA++E G G+I+L++ Sbjct: 296 IHHRFPLEQAAQAHAMME-GSTHIGKIMLEL 325 >gi|300312848|ref|YP_003776940.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 94.7 bits (234), Expect = 8e-21, Method: Compositional matrix adjust. Identities = 102/334 (30%), Positives = 149/334 (44%), Gaps = 45/334 (13%) Query: 4 QAIRYHQYGPPDVMALEAIAQFPLEPGQVRVSLRAAGVAPLDAKLRAGLLQAQLALTLPK 63 +AIR + G P+VM + G+VR+ A G+ +D RAGL LP Sbjct: 3 KAIRMSKTGGPEVMEYVDVEVGEPGAGEVRIRHAAVGLNFIDCYFRAGLYPQ----PLPA 58 Query: 64 VPGRDGAGVI--VESRAEGLPVGEPVCVMADMFGQGSYADSIVVPAARVVRQPSTLSTRQ 121 G++ AG I V + G VG+ V G+YA+ V+PA +V+ P +S Sbjct: 59 GLGQEAAGTIEAVGAGVSGFKVGDRVAYAGRP--NGAYAEERVMPADILVKLPDAISFDT 116 Query: 122 AAALLQPGASAWIPIVRTAQVRSGMKVLVHAGSGAVGSLMIQLAAHLGAEVWATCSSANR 181 AAA++ G + + +T V+ G +L HA +G +G + Q A LG + T SS + Sbjct: 117 AAAMMLQGMTVQYLLRQTYAVQPGDTILFHAAAGGIGLIACQWARALGVNLIGTVSS-DE 175 Query: 182 DDVLAL--GATGVIAYDKEDF-------SALRGVDVVFDLVGGDTHDRSYPVLKPGGQLV 232 LAL GA VI Y E+F + RGV VV+D +G DT S L+P G +V Sbjct: 176 KAALALQHGAAHVINYKTENFVERVKELTGGRGVQVVYDSIGKDTFLGSLDCLRPRGMMV 235 Query: 233 WLVAAP----------IRERGEEFKVR-------VVRAMIDDAPECLHAVA---KLAEER 272 A + RG F R RA +D L + K++ E Sbjct: 236 SFGNASGPVAPFGVSELASRGSLFLTRPSLGNYTSTRAELDATAGDLFDMVLGNKISIE- 294 Query: 273 VWVPTVGQCFPLAEAARAHALLESGKAARGRIVL 306 + Q + L + A+AHA LE G+ G +L Sbjct: 295 -----INQRYALKDVAQAHADLE-GRKTTGSTIL 322 >gi|300312794|ref|YP_003776886.1| NADPH:quinone oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 342 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 95/337 (28%), Positives = 145/337 (43%), Gaps = 33/337 (9%) Query: 2 NTQAIRYHQYGPPDVMALEAIAQFPLEPGQVRVSLRAAGVAPLDAKLRAGLL----QAQL 57 +AI+ H +G P+V+ E L+PG+V V + A G+ P D LR G + Q Sbjct: 5 TMKAIQQHAFGGPEVLRYEDAPVPELQPGEVLVRVHAVGLNPPDWYLREGYKMLPPEWQP 64 Query: 58 ALTLPKVPGRDGAGVI--VESRAEGLPVGEPVCVMADM-----FGQGSYADSIVVPAARV 110 + P + G D +GV+ V + VG+ V M G +YA + V A+ + Sbjct: 65 TVAFPLILGTDISGVVEAVADDVKDFAVGDEVYSMVRFPSGLAGGSRAYAQYVSVAASEI 124 Query: 111 VRQPSTLSTRQAAALLQPGASAWIPIVRTAQ-VRSGMK-------------VLVHAGSGA 156 +P +S QAAA +AW ++ +++ ++ V+V+ +G Sbjct: 125 ALKPQRISHAQAAAAPMSLLTAWQFMIELGHDIQNPLQPHRHEPVPLKDKTVVVNGAAGG 184 Query: 157 VGSLMIQLAAHLGAEVWATCSSANRDDVLALGATGVIAYDK---EDFSALRGVDVVFDLV 213 VG +QLA GA V A S + + LGA I Y + ED L VD+V D V Sbjct: 185 VGHFALQLAKWKGARVIAIGSGGHEAFLRDLGADEFIDYTRSAAED--VLSDVDLVIDAV 242 Query: 214 GGDTHDRSYPVLKPGGQL--VWLVAAPIRERGEEFKVRVVRAMIDDAPECLHAVAKLAEE 271 GG R LK GG L ++ + E V V + + L +A L + Sbjct: 243 GGPASGRFLRTLKRGGALFPIFPLGFSGAEEAARLGVNVSTTQVRSSGAQLQQLAGLLDS 302 Query: 272 RVWVPTVGQCFPLAEAARAHALLESGKAARGRIVLDI 308 V + F L +A +AH G RG+IVL + Sbjct: 303 GVVRVAIDSTFALQDAGKAHERAAQGH-IRGKIVLSV 338 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 924,298 Number of extensions: 35942 Number of successful extensions: 182 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 179 Number of HSP's successfully gapped: 5 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)