BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0120.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 551 e-158 gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 227 7e-61 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 121 5e-29 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 551 bits (1419), Expect = e-158, Method: Compositional matrix adjust. Identities = 269/309 (87%), Positives = 287/309 (92%), Gaps = 6/309 (1%) Query: 1 MVNRRQFLAGSLLACTAARSAIAQSIPNSAGDQPPRLKAPALACDSHHHIYDARFPVSPH 60 M++RR F+AG++LAC RSA AQS+PNSAG QPPRLKAP LACDSHHHIYDARFPVSPH Sbjct: 1 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 Query: 61 WRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 WRGGRPDGATVADYR LMAKLGIAR+VVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD Sbjct: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 Query: 121 DVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLMSGAQIA 180 DV+L QLKEMD+LGVRGVRVNFLS QSWG+TTV+RL RTA+R+APLGWHVQVLMSGAQIA Sbjct: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 Query: 181 EHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEPYADTLQGPP 240 +HEAVLA LPT VVIDHLGRIPQP GLDHPGAQA+LRLLAKGNTWIKLSEPYADTLQGPP Sbjct: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 Query: 241 DYPDTSLLARX------QRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRHQILVS 294 DYPDTSLLA+ QRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALR QILVS Sbjct: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 Query: 295 NPASLFGFS 303 NPA+LFGF+ Sbjct: 301 NPAALFGFA 309 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 227 bits (579), Expect = 7e-61, Method: Compositional matrix adjust. Identities = 135/314 (42%), Positives = 178/314 (56%), Gaps = 18/314 (5%) Query: 3 NRRQFL------AGSLLACTAARSAIAQS--IPNSAGDQPPRLKAPALACDSHHHIYDAR 54 +RR FL A SL A A+AQ+ P+S+G Q + P ACD H HIYD R Sbjct: 6 SRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMHIYDPR 65 Query: 55 FPVSPHWRGGRPDGATVADYRRLMAKLGIARNVVVQPSTYGSDNRCLLDALTQFGAQARG 114 F +P + ATVA YR+L +LG RNVVV PS YG DN C LDAL Q G ARG Sbjct: 66 FAWAPGAKLVHAP-ATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGRSARG 124 Query: 115 IVVIDDDVELAQLKEMDQLGVRGVRVNFLSPQSWGITTVDRLLRTAKRVAPLGWHVQVLM 174 + V++D V A+L+ + Q GVRG+R N +TTV+ + A+R+APLGWH+Q M Sbjct: 125 VAVVNDQVSDAELQRLHQAGVRGLRFNL---AVGSVTTVEMMEPLARRMAPLGWHLQANM 181 Query: 175 SGAQIAEHEAVLAGLPTPVVIDHLGRIPQPQGLDHPGAQAILRLLAKGNTWIKLSEPYAD 234 + H A+LA LP P+V DH RIP HP + L+ + +KLS Y Sbjct: 182 PNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYLY 241 Query: 235 TLQGPPDYPDTSLLARX------QRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALR 288 + +G PDY D + LAR + +WGSDWPHPTE+ KPDDA L D++ +W + Sbjct: 242 SKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEMV 301 Query: 289 HQILVSNPASLFGF 302 +LV+NPA L+ F Sbjct: 302 RTVLVANPARLYQF 315 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 121 bits (304), Expect = 5e-29, Method: Compositional matrix adjust. Identities = 90/281 (32%), Positives = 123/281 (43%), Gaps = 15/281 (5%) Query: 33 QPPRLKAPALACDSHHHIYDARFPVSPHWRGGRPDGATVADYRRLMAKLGIARNVVVQPS 92 QP A L D+H H++ P++ R TV Y + G++ V+VQPS Sbjct: 3 QPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQPS 62 Query: 93 TYGSDNRCLLDALTQFGAQARGIVVIDDDVELAQLKEMDQLGVRGVRVNFLS----PQSW 148 GSDNR LL AL ++ + RGI ++D DV QL + GV G+R N + P Sbjct: 63 FLGSDNRYLLAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFT 122 Query: 149 GITTVDRLLRTAKRVAPLGWHVQVLMSGAQIAEHEAVLAGLPTPVVIDHLGRIPQPQGLD 208 G+ T R+A LGW V++ + L + VV+DH GR+ G+D Sbjct: 123 GVWR-----STLARIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVD 177 Query: 209 HPGAQAILRLLAKGNTWIKLSEPYAD--TLQGPPDYPDTSLLARX----QRTLWGSDWPH 262 PG + +L A G W+KLS Y + QG L R QR LWGSDWPH Sbjct: 178 DPGFRYLLSSAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPH 237 Query: 263 PTEKIKPDDAVLFDLLAAWAPDPALRHQILVSNPASLFGFS 303 + D + D R I A LFG + Sbjct: 238 TQHESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGIN 278 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,031,348 Number of extensions: 46330 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 139 Number of HSP's successfully gapped: 3 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)