BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0119.p (440 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 822 0.0 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 345 4e-96 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 310 1e-85 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 309 3e-85 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 297 1e-81 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 822 bits (2124), Expect = 0.0, Method: Compositional matrix adjust. Identities = 403/428 (94%), Positives = 418/428 (97%) Query: 1 METIQDDKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDIGLSPKMFGF 60 METIQ++KRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHD+GLSPKMFGF Sbjct: 1 METIQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF 60 Query: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMVTWGLISACTAFVQGPTSLYFVRFILG 120 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIM+TWGLISACTA VQGP SLYFVRFILG Sbjct: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILG 120 Query: 121 AAEAGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 AAEAGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW Sbjct: 121 AAEAGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 Query: 181 HWLFLLEGLPTVLLGVVCLFFLTDRPDQARWLNDAQRQWLVATMNKEAASRTAKQHIPLW 240 HWLF+LEGLPTV+LG+VCL FLTDRPDQARWL+D QRQWL TMNKEAASR AKQHIPLW Sbjct: 181 HWLFMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLW 240 Query: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLTVMQTGLVNSIPYLVASVLMVW 300 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGL+VMQTGLVNSIPYL+ASVLMVW Sbjct: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW 300 Query: 301 WGRHSDRTGERRWHTAIPLILIGGGLALASLANTLAPVVLLLTMVLIGAYSFKGPFWAMA 360 WGRHSDRTGERRWHTAIPL+LIGGGLALASLAN+LAPVVLLLTMVLIGAYSFKGPFWAMA Sbjct: 301 WGRHSDRTGERRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMA 360 Query: 361 STWLATGSAAAGLAGINAVSNLIGGGLMVNAYGWIKDATGSYAMALLPLVGLSVVSVAIL 420 S+WLATGSAAAGLAGINAVSNLIGGGLMVN YGW+KDATGSYAMALLP+VGLSVVSV IL Sbjct: 361 SSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGIL 420 Query: 421 LVLSRKSR 428 L LSRKSR Sbjct: 421 LFLSRKSR 428 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 345 bits (885), Expect = 4e-96, Method: Compositional matrix adjust. Identities = 179/422 (42%), Positives = 261/422 (61%), Gaps = 1/422 (0%) Query: 11 EQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDIGLSPKMFGFASSLFFIAYF 70 E T++KV RL+PF++LCY+ +++DR N+G AAL MN D+GL+P FG + LFFI YF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 71 IFEVPSNLALQKFGARKWLARIMVTWGLISACTAFVQGPTSLYFVRFILGAAEAGFFPGV 130 FE+PSNLAL +FGAR W++RIM++WGLIS TAFV GP S RF+LG AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 131 VLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFLLEGLP 190 LY T W P A+R + A F++ IP A+ + SP+S L+++ G+ G +GW L LLE LP Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 191 TVLLGVVCLFFLTDRPDQARWLNDAQRQWLVATMNKEAASRTAKQHIPLWKLFSQPHVWG 250 VLLGV+CLF L DRP +A+WL+ ++QWL ++ E A+ L F+ V+ Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 Query: 251 MALVCACASAAGSVLNVWQPQLLKSFGLTVMQTGLVNSIPYLVASVLMVWWGRHSDRTGE 310 +A C + +W PQ+++ FGL Q GLV +IPYLV +V M W R ++R+ Sbjct: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSER 319 Query: 311 RRWHTAIPLILIGGGLALASLANTLAPVVLLLTMVLIGAYSFKGPFWAMASTWLATGSAA 370 R + A ++L L +++ +T +L +T+ + SF+ FWA+ S +L +AA Sbjct: 320 RLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAA 379 Query: 371 AGLAGINAVSNLIGGGLMVNAYGWIKDATGSYAMALLPLVGLSVVSVAILLVLSRKSRVA 430 GLA I ++ NL GG + + G+I++AT + L+ + G ++ I L L SRV Sbjct: 380 GGLALIVSIGNL-GGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSRVP 438 Query: 431 AT 432 T Sbjct: 439 PT 440 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 310 bits (795), Expect = 1e-85, Method: Compositional matrix adjust. Identities = 169/422 (40%), Positives = 255/422 (60%), Gaps = 11/422 (2%) Query: 10 LEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDIGLSPKMFGFASSLFFIAY 69 E T KVTWRLLP L LCY+ +++DR N+G A LQM D+ S ++G + +FF+ Y Sbjct: 16 FEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGY 75 Query: 70 FIFEVPSNLALQKFGARKWLARIMVTWGLISACTAFVQGPTSLYFVRFILGAAEAGFFPG 129 F+FEVPSN+ L K GAR W+ARIM+TWG+IS A+V P Y +RF+LG AEAGFFPG Sbjct: 76 FVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPG 135 Query: 130 VVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFLLEG 188 V+LYLTYW P+A R +I ALFM I + + P+S ++ M G+ G GW W+F+LE Sbjct: 136 VILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEA 195 Query: 189 LPTVLLGVVCLFFLTDRPDQARWLNDAQRQWLVATMNKEAASRTAKQHIPLWKLFSQPHV 248 +P+++LGV+ + ++ DR A WL++ +++ L A ++ E + K+H+ L +F+ V Sbjct: 196 IPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHE---ESQKEHLSLGAMFANGRV 252 Query: 249 WGMALVCACASAAGSVLNVWQPQLLKSFGLTV-MQTGLVNSIPYLVASVLMVWWGRHSDR 307 W AL+ C ++ W P ++K+ G++ + GL+ +IPY A+ MV GR +D Sbjct: 253 WLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADA 312 Query: 308 TGERRWHTAIPLILIGGGLALAS-LANTLAPVVLLLTMVLIGAYSFKGPFWAMASTWLAT 366 ERRWH AIP + GL L++ + + LT+ +G + FW++ + +L+ Sbjct: 313 RRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSG 372 Query: 367 GSAAAGLAGINAVSNLIGGGLMVNAY--GWIKDATGSYAMALLPLVGLSVVSVAILLVLS 424 +AAAG+A IN++ NL G V+ + GW+KDAT S + L V+ + L L Sbjct: 373 TAAAAGIALINSLGNLAG---FVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLP 429 Query: 425 RK 426 K Sbjct: 430 AK 431 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 309 bits (791), Expect = 3e-85, Method: Compositional matrix adjust. Identities = 169/415 (40%), Positives = 256/415 (61%), Gaps = 15/415 (3%) Query: 7 DKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDIGLSPKMFGFASSLFF 66 + + + +KV WR++PFLMLCY+ A++DR N+G A LQM+ D+G S +FG + LFF Sbjct: 14 NDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFF 73 Query: 67 IAYFIFEVPSNLALQKFGARKWLARIMVTWGLISACTAFVQGPTSLYFVRFILGAAEAGF 126 I YF+FEVPSN+ + K GAR W+ARIM+TWG++SA FVQ T Y +RF+LG AEAGF Sbjct: 74 IGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGF 133 Query: 127 FPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFL 185 +PG++LYLTYW PS RA+++A+FM IP A + +P+S ++ GGL GW W+FL Sbjct: 134 YPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFL 193 Query: 186 LEGLPTVLLGVVCLFFLTDRPDQARWLNDAQRQWLVATMNKEAASRTAKQHIPLWKLFSQ 245 +E +P VL+GV + +L + A+WLND ++ L A ++ +A + +K + Sbjct: 194 IEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFG--AIVKD 251 Query: 246 PHVWGMALVCACASAAGSVLNVWQPQLLKSFGLT-VMQTGLVNSIPYLVASVLMVWWGRH 304 VW M L+ L +W P L+K+ G+ ++ GL+++IP+ A + M GR Sbjct: 252 ARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRS 311 Query: 305 SDRTGERRWHTAIPLILIGGGLALASL-ANTLAPVVLLLTMVLIGAYSFKGPFWAMASTW 363 +DR ERRWH IP ++ G G A+L A+ A + L++ G + FW++ + + Sbjct: 312 ADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAF 371 Query: 364 LATGSAAAGLAGINAVSNLIGGGLMVNAY--GWIKD-----ATGSYAMALLPLVG 411 L+ +AA G+A IN+V NL G V+ Y G++KD ATG Y +A++ +VG Sbjct: 372 LSGAAAAVGIAAINSVGNLAG---FVSPYLIGYLKDLTHNNATGMYMLAVMLVVG 423 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 297 bits (760), Expect = 1e-81, Method: Compositional matrix adjust. Identities = 171/420 (40%), Positives = 253/420 (60%), Gaps = 10/420 (2%) Query: 12 QETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDIGLSPKMFGFASSLFFIAYFI 71 + T RK+ WRL+PFL+ ++ A+IDR N+G A LQM D+ S ++G + +FFI YFI Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 72 FEVPSNLALQKFGARKWLARIMVTWGLISACTAFVQGPTSLYFVRFILGAAEAGFFPGVV 131 FEVPSNL L+K GARK LARI + WGL S A+V PTS Y +RF+LG EAGFFPGVV Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 132 LYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFLLEGLP 190 LYLTYW P+ RAR+ LFM + A V P++ L++ M+G+G L W WLF+LEG+P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 191 TVLLGVVCLFFLTDRPDQARWLNDAQRQWLVATMNKEAASRTAKQHIPLWKLFSQPHVWG 250 ++L G L +L +RP A+WL++A++ + + E + +H + VW Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAE--NSAPGKHFSFKDACTNYRVWI 259 Query: 251 MALVCACASAAGSVLNVWQPQLLKSFGLT-VMQTGLVNSIPYLVASVLMVWWGRHSDRTG 309 A V C + + + W P ++K G+ +Q GL+++IP++ ++ M+W G HSD+TG Sbjct: 260 CAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTG 319 Query: 310 ERRWHTAIPLILIGGGLALASLANTLAPVVLL-LTMVLIGAYSFKGPFWAMASTWLATGS 368 ERR H+AI ++ GL + A + L LT+ IG + FW++ +LA + Sbjct: 320 ERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTA 379 Query: 369 AAAGLAGINAVSNLIGGGLMVNAY--GWIKDATGSYAMALLPLVGLSVVSVAILLVLSRK 426 AA G+A IN++ NL G V Y G +K +TGS + L + L +V+ +++V RK Sbjct: 380 AAGGIALINSIGNLAG---FVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.139 0.443 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,177,256 Number of extensions: 41944 Number of successful extensions: 298 Number of sequences better than 1.0e-08: 21 Number of HSP's gapped: 263 Number of HSP's successfully gapped: 21 Length of query: 440 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 352 Effective length of database: 1,203,190 Effective search space: 423522880 Effective search space used: 423522880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)