BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0115.p (435 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 688 0.0 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 313 2e-86 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 306 2e-84 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 291 6e-80 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 269 4e-73 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 688 bits (1776), Expect = 0.0, Method: Compositional matrix adjust. Identities = 344/358 (96%), Positives = 350/358 (97%) Query: 23 EEAIMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 82 E+A MKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 83 FFEIPSNLALHRFGARMWISRIMISWGVISMATAFVVGPKSFALARFLLGMAEAGFTPGI 142 FFEIPSNLALHRFGARMWISRIMISWG+ISMATAFVVGPKSFALARFLLGMAEAGFTPGI Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 143 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 202 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 203 AVLLGVLCLFLLPDRPSKAAWLTPSEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 262 AVLLGV+CLFLLPDRP+KA WL+ EKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 Query: 263 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSAH 322 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRS Sbjct: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSER 319 Query: 323 RLFFVAGAIVLAALSLAISAFLHTPLLKMLAITIAVASILSFQATFWAIPSGFLTGPA 380 RLFFVAGAIVLAALSL ISAFLHTPLLKMLAIT+AVASILSFQATFWAIPSGFLTG A Sbjct: 320 RLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRA 377 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 313 bits (801), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 154/359 (42%), Positives = 227/359 (63%), Gaps = 1/359 (0%) Query: 23 EEAIMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 82 E+ ++KV RL+PF++LCY+ +++DR N+G AAL MN DLGL+P FG + LFFI YF Sbjct: 11 EQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYF 70 Query: 83 FFEIPSNLALHRFGARMWISRIMISWGVISMATAFVVGPKSFALARFLLGMAEAGFTPGI 142 FE+PSNLAL +FGAR W++RIMI+WG+IS TA V GP S RF+LG AEAGF PG+ Sbjct: 71 IFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFFPGV 130 Query: 143 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 202 LY T W P A+R + A F++ IP A+ + SP+S L+++ G+ G +GW L +LE LP Sbjct: 131 VLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLEGLP 190 Query: 203 AVLLGVLCLFLLPDRPSKAAWLTPSEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 262 V+LG++CL L DRP +A WL+ ++QWL ++ E AA+ L F+ V+ Sbjct: 191 TVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWG 250 Query: 263 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSAH 322 +A C + +W PQ+++ FGL Q GLV +IPYL+ +V M W R ++R+ Sbjct: 251 MALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRHSDRTGE 310 Query: 323 RLFFVAGAIVLAALSLAISAFLHTPLLKMLAITIAVASILSFQATFWAIPSGFL-TGPA 380 R + A ++L LA+++ ++ +L +T+ + SF+ FWA+ S +L TG A Sbjct: 311 RRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMASSWLATGSA 369 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 306 bits (785), Expect = 2e-84, Method: Compositional matrix adjust. Identities = 164/361 (45%), Positives = 230/361 (63%), Gaps = 6/361 (1%) Query: 23 EEAIMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 82 E+A KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 83 FFEIPSNLALHRFGARMWISRIMISWGVISMATAFVVGPKSFALARFLLGMAEAGFTPGI 142 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 143 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 201 LY T W+P A RGK TA F+ GI ++ +IG PLSG +M + G+WG GWQ + +LEA+ Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 202 PAVLLGVLCLFLLPDRPSKAAWLTPSEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 261 P+++LGV+ + + DR A WL+ EK+ LE +S E++ + L F N RV+ Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEES---QKEHLSLGAMFANGRVW 253 Query: 262 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLANRS 320 A FC ++G + WLP II+ G+ VGL+ AIPY A AM L R A+ Sbjct: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 Query: 321 AHRLFFVAGAIVLAALSLAISA-FLHTPLLKMLAITIAVASILSFQATFWAIPSGFLTGP 379 R + VA + + L +S + H L M ++T+A IL+ FW++P+ FL+G Sbjct: 314 RERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGT 373 Query: 380 A 380 A Sbjct: 374 A 374 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 291 bits (745), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 151/361 (41%), Positives = 231/361 (63%), Gaps = 9/361 (2%) Query: 22 SEEAIMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGY 81 S++ + KKV R++PF++LCYV++YLDR+NVGFA L M++DLG + + FGLGAGLFFIGY Sbjct: 17 SKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGY 76 Query: 82 FFFEIPSNLALHRFGARMWISRIMISWGVISMATAFVVGPKSFALARFLLGMAEAGFTPG 141 F FE+PSN+ +H+ GAR+WI+RIMI+WG++S A FV F + RFLLG+AEAGF PG Sbjct: 77 FLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPG 136 Query: 142 IYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEA 200 I LY T WFP R K A F+ GIPVA I+G+PLSG +M+ H G +GWQ + L+EA Sbjct: 137 IILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEA 196 Query: 201 LPAVLLGVLCLFLLPDRPSKAAWLTPSEKQWLENELSTEQNVLAARH--GNKLKDAFTNW 258 +PAVL+GV + L + A WL EK L+ ++ + ++H G +KDA Sbjct: 197 IPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDA---- 252 Query: 259 RVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAIPYLVGAVAMTLWARLA 317 RV+ + F ++G + LW+P +++ G+ + ++GL++AIP+ +AM L R A Sbjct: 253 RVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSA 312 Query: 318 NRSAHRLFFVAGAIVLAALSLAISA-FLHTPLLKMLAITIAVASILSFQATFWAIPSGFL 376 +R R + + ++ + +A F + + ++++A A +L+ FW++P+ FL Sbjct: 313 DRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFL 372 Query: 377 T 377 + Sbjct: 373 S 373 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 269 bits (687), Expect = 4e-73, Method: Compositional matrix adjust. Identities = 157/370 (42%), Positives = 224/370 (60%), Gaps = 17/370 (4%) Query: 19 AAVSEEAIMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFF 78 +AV + + KKV +++P++LL YV++YLDR+NVGFA L+M DLG++ + +GLGAG+FF Sbjct: 8 SAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFF 67 Query: 79 IGYFFFEIPSNLALHRFGARMWISRIMISWGVISMATAF---------VVGPKSFALARF 129 +GYF FE+PSNL LHR GA++WI+RIMI+WG+IS AF V F RF Sbjct: 68 LGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRF 127 Query: 130 LLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMW-G 188 LLG +EAGF PG+ LY T W+P A + A L+G P + I+G P+SGALM+ G Sbjct: 128 LLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDS 187 Query: 189 FKGWQVLLLLEALPAVLLGVLCLFLLPDRPSKAAWLTPSEKQWLENELSTEQNVLAARHG 248 GWQ + ++EALPA LLG++ LF+L + + A WL P EK L L+ E + Sbjct: 188 LAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK---EKVN 244 Query: 249 NKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPS-HQVGLVAAIPYLVGA 307 LK + ++ LA +IGS I W P IIR+ G+ S +G+++AIPY++GA Sbjct: 245 VPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGA 304 Query: 308 VAMTLWARLANRSA-HRLFFVAGAIVLAALSLAI-SAFLHTPLLKMLAITIAVASILSFQ 365 AM R A ++ HR + VA ++V A L+L + + F H + + I IAV L+ Sbjct: 305 TAMVFITRHAEKAGKHRSYSVAVSMV-AGLALVLCTLFPHNIYITLAGIAIAVGGSLTSI 363 Query: 366 ATFWAIPSGF 375 A FW P + Sbjct: 364 ALFWGFPGAY 373 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,339,026 Number of extensions: 57256 Number of successful extensions: 314 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 278 Number of HSP's successfully gapped: 20 Length of query: 435 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 347 Effective length of database: 1,203,190 Effective search space: 417506930 Effective search space used: 417506930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)