BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0114.p (315 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 546 e-157 gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 231 6e-62 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 87 2e-18 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 546 bits (1407), Expect = e-157, Method: Compositional matrix adjust. Identities = 267/315 (84%), Positives = 288/315 (91%) Query: 1 MQDLPSRRAFLRHSTRLALGLGAIAQLPLARAQTEPYAHSAGTQHAASRLPADACDCHMH 60 MQDLPSRR FLR S +LAL LGA LP A AQ + Y HS+G+Q AASRLP DACDCHMH Sbjct: 1 MQDLPSRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMH 60 Query: 61 IYDPRFAWAPGAKLLHAPATVPMYRRLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGA 120 IYDPRFAWAPGAKL+HAPATV MYR+LQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLG Sbjct: 61 IYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGR 120 Query: 121 TARGVAVVNDQVSDAELQRLHQAGVRGLRFNLALGSVTTVEMMEPLARRVAALGWHLQVN 180 +ARGVAVVNDQVSDAELQRLHQAGVRGLRFNLA+GSVTTVEMMEPLARR+A LGWHLQ N Sbjct: 121 SARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARRMAPLGWHLQAN 180 Query: 181 MPNDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMREQRASVKLSGAYL 240 MPN+VLLA+RA+L+ LPVPLVFDHFARIPLQA HPVFDFVTGLMRE+RASVKLSGAYL Sbjct: 181 MPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYL 240 Query: 241 YSKRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHKPDDARLLDVMTTWLGSDAM 300 YSKRGAPDY+DVAPLAR LVQ AP+Q+VWGSDWPHPTEQHKPDDARLLDVM +WLGS+ M Sbjct: 241 YSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEM 300 Query: 301 LRTILVDNPARLYQF 315 +RT+LV NPARLYQF Sbjct: 301 VRTVLVANPARLYQF 315 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 231 bits (589), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 137/313 (43%), Positives = 181/313 (57%), Gaps = 12/313 (3%) Query: 7 RRAFLRHSTRLALGLGAIAQLPLARAQTEPYAHSAGTQHAASRLPADACDCHMHIYDPRF 66 RR+F+ A + A A + A AQ+ P +SAG+Q + P ACD H HIYD RF Sbjct: 4 RRSFI------AGAMLACAGVRSATAQSVP--NSAGSQPPRLKAPMLACDSHHHIYDARF 55 Query: 67 AWAPGAKLLHAP-ATVPMYRRLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGATARGV 125 +P + ATV YR L +LG R+VVV PS YG DN C LDAL Q GA ARG+ Sbjct: 56 PVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGI 115 Query: 126 AVVNDQVSDAELQRLHQAGVRGLRFNLALG---SVTTVEMMEPLARRVAALGWHLQVNMP 182 V++D V +L+ + + GVRG+R N VTTVE + A R+A LGWH+QV M Sbjct: 116 VVIDDDVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMS 175 Query: 183 NDVLLANRALLSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMREQRASVKLSGAYLYS 242 + + A+L+ALP +V DH RIP G HP V L+ + +KLS Y + Sbjct: 176 GAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADT 235 Query: 243 KRGAPDYEDVAPLARTLVQTAPSQVVWGSDWPHPTEQHKPDDARLLDVMTTWLGSDAMLR 302 +G PDY D + LA+ +Q AP + +WGSDWPHPTE+ KPDDA L D++ W A+ + Sbjct: 236 LQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQ 295 Query: 303 TILVDNPARLYQF 315 ILV NPA L+ F Sbjct: 296 QILVSNPAALFGF 308 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 87.0 bits (214), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 79/268 (29%), Positives = 117/268 (43%), Gaps = 15/268 (5%) Query: 55 CDCHMHIYDPRFAWAPGAKLLHAPA---TVPMYRRLQQRLGTTRNVVVTPSAYGVDNSCT 111 D H H++ A + +AP TV Y G + V+V PS G DN Sbjct: 14 IDTHAHVFTRDLPLADVRR--YAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71 Query: 112 LDALAQLGATARGVAVVNDQVSDAELQRLHQAGVRGLRFNLALGSVT--TVEMMEPLARR 169 L ALA+ RG+A+V+ VS+ +L L GV G+RFNL G+ + R Sbjct: 72 LAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTGVWRSTLAR 131 Query: 170 VAALGWHLQVNMPNDVLLANRAL--LSALPVPLVFDHFARIPLQAGPGHPVFDFVTGLMR 227 +A LGW QV + + + R L L A+ + +V DHF R+ G P F ++ Sbjct: 132 IAQLGW--QVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYLLSSAA 189 Query: 228 EQRASVKLSGAYLYSKRGAPDYEDVAPLARTLVQT--APSQVVWGSDWPHPTEQHKPDDA 285 + VKLS + Y GA + A A L++ +++WGSDWPH + D Sbjct: 190 GGQVWVKLSAS--YRNGGAEQGQRFARQAWPLLREHFGAQRLLWGSDWPHTQHESLTDYG 247 Query: 286 RLLDVMTTWLGSDAMLRTILVDNPARLY 313 T + + R I A+L+ Sbjct: 248 HSWQQFTQLVSDEEQRRIITGQTAAQLF 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.134 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,083,007 Number of extensions: 48390 Number of successful extensions: 164 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 160 Number of HSP's successfully gapped: 3 Length of query: 315 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 230 Effective length of database: 1,217,395 Effective search space: 280000850 Effective search space used: 280000850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)