BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0105.p (477 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 798 0.0 gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 330 1e-91 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 111 1e-25 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 102 6e-23 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 91 2e-19 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 798 bits (2062), Expect = 0.0, Method: Compositional matrix adjust. Identities = 406/455 (89%), Positives = 421/455 (92%), Gaps = 10/455 (2%) Query: 1 VIGRLRESAIALNPQYRLYIHLLSEEETEPYLQALEGQSPHSLPLYGVPFSIKDNIDLAG 60 V+ RLRESA ALNP YRLYIHLLS+ ETEPYLQALE QSPHSLPLYGVPFSIKDNIDLAG Sbjct: 26 VMQRLRESAAALNPTYRLYIHLLSQAETEPYLQALEQQSPHSLPLYGVPFSIKDNIDLAG 85 Query: 61 IPTTAACPAFSYVPRESATLVAQLIALGAIPIGKCNLDQFATGLNGTRSPYGACRNSVHP 120 +PTTAACPAFSYVPRESATLVAQLIALGAIP+GKCNLDQFATGLNGTRSPYGACRNSVHP Sbjct: 86 VPTTAACPAFSYVPRESATLVAQLIALGAIPVGKCNLDQFATGLNGTRSPYGACRNSVHP 145 Query: 121 DYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPAC 180 DYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPAC Sbjct: 146 DYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPAC 205 Query: 181 RTLDCVTFFTATAAEASHLLALCTQAMQPDERDEYSRANLPHNDAAAFQPPRKGLRVGVP 240 RTLDCVTFFTATAAEASHLLALCTQ+ QPDERDEYSRANLPHNDA+AFQPP +GLR GVP Sbjct: 206 RTLDCVTFFTATAAEASHLLALCTQSGQPDERDEYSRANLPHNDASAFQPPPQGLRFGVP 265 Query: 241 RQLEFLGCSESPSLFVEALGQLEAIGGVSVSIDFSPFLEAARLLYEGPWVAERYSTIEAL 300 RQLEFLGCSESPSLFVEAL Q+EAIGGV+V IDFSPFL AARLLYEGPWVAERYSTIEAL Sbjct: 266 RQLEFLGCSESPSLFVEALAQMEAIGGVAVPIDFSPFLAAARLLYEGPWVAERYSTIEAL 325 Query: 301 LHEKPEEILPVIRDVLRKAPQFSAVDTFRAQYRLQQLKAECDRILATVDCVVTPAYPRPV 360 L E+P++ILPVIRDVLRKAPQ+SAVDTFRAQYRLQQLKAECDRILATVDCVVTPAYPRPV Sbjct: 326 LQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQQLKAECDRILATVDCVVTPAYPRPV 385 Query: 361 TLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPMNFMKNGLPWGITVFGPAFSDQRLLG 420 TLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPMNFMKNGLPWGITVFG AFSDQ LLG Sbjct: 386 TLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPMNFMKNGLPWGITVFGRAFSDQALLG 445 Query: 421 VADALQGEEFTGVHARGHQVAAIAAGRRIHRRSRA 455 VADALQ H R + R+I R RA Sbjct: 446 VADALQ-----RAHGR-----PLVGQRQIEDRRRA 470 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 330 bits (847), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 181/389 (46%), Positives = 259/389 (66%), Gaps = 10/389 (2%) Query: 41 HSLPLYGVPFSIKDNIDLAGIPTTAACPAFSYVPRESATLVAQLIALGAIPIGKCNLDQF 100 S+PL+G+PF++KDNID+AG+PTTAACP FS V SAT+V +L A GA+ +GK NLDQF Sbjct: 79 ESMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVVQRLQAAGALLVGKTNLDQF 138 Query: 101 ATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAALNN 160 ATGL G RSPYGA RN++ PDY SGGSSSGS++AVALG FALGTDTAGSGR+PAA N+ Sbjct: 139 ATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVMFALGTDTAGSGRIPAAFNH 198 Query: 161 LVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQAMQPDERDEYSRANL 220 LVGLK ++GL+S G+VPACRTLDC++ F A+A H+L + + + L Sbjct: 199 LVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVL----HSAAGYDAADAYARKL 254 Query: 221 PHNDAAAFQPPRKGLRVGVPRQLEFLGCSESPSLFVEALGQLEAIGGVSVS-IDFSPFLE 279 P R+G R+ +P EF G + + L ++ + GV+++ I F+PF + Sbjct: 255 PM-----LGLKRRGYRIAIPAATEFYGDLAAQDAWDRTLARIAELPGVTLARIPFAPFAD 309 Query: 280 AARLLYEGPWVAERYSTIEALLHEKPEEILPVIRDVLRKAPQFSAVDTFRAQYRLQQLKA 339 AARLLY+GPWVAER + + ++P+ + PV+R++ +A + AV F+ QYRL +L+ Sbjct: 310 AARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAADYDAVAAFQGQYRLAELRR 369 Query: 340 ECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPMNFMK 399 + +++L D ++ P T+ E++ PV+RNS LG+YTNF+N++D +A+A P + Sbjct: 370 QAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYTNFVNLMDMSALAIPGLWRN 429 Query: 400 NGLPWGITVFGPAFSDQRLLGVADALQGE 428 +GLP G+T+ GPA +D L LQ + Sbjct: 430 DGLPAGVTLIGPAGADHLLAEAGARLQHQ 458 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 111 bits (278), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 131/436 (30%), Positives = 199/436 (45%), Gaps = 41/436 (9%) Query: 15 QYRLYIHLLSE-EETEPYLQALEGQSPHSLPLYGVPFSIKDNIDLAGIPTTAACPAF-SY 72 Q+ L +L + E+ + L+ Q H PL G+P +IKDNID+AGI +TA A + Sbjct: 36 QWDLNCYLQRDPEQLRSQARMLDEQGGHG-PLSGIPIAIKDNIDVAGIASTAGTAALRNN 94 Query: 73 VPRESATLVAQLIALGAIPIGKCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSS 132 VP+ AT+V L GA+ GK N+ + A G+ +G CRN + +GGSS G Sbjct: 95 VPQRHATVVEYLFGAGALHAGKANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCG 154 Query: 133 LAVALGLSSFALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTAT 192 +A L+ A+GTDT GS R+PAAL +VGL+ T S GVVP T D + Sbjct: 155 ALLAARLAPAAVGTDTGGSVRIPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARS 214 Query: 193 AAEASHLLALCTQAMQPDERDEYSRANLPHNDAAAFQPPRKGLRVGVPRQLEFLGCSESP 252 A+ +AL Q M RD LP + LR+GV R + G E Sbjct: 215 VAD----VALLDQIMA-GSRDSAPLLALPPSQ----------LRLGVLRNPSWQGLEEEV 259 Query: 253 SLFVE-ALGQLEAIGGVSVSID---FSPFLEAA-------RLLYEGP---WVAERYSTIE 298 AL QL G V +D +P AA L + P ++ I+ Sbjct: 260 EANANAALMQLREAGVELVDVDLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIK 319 Query: 299 ALLHE-KPEEILPVIRDVLRK-APQFSAVDTFRAQYRLQQLKAECDRILATVDCVVTPA- 355 +L E ++ +I+ L + P+ + + +Q L +C R VD ++ P Sbjct: 320 RVLAEVGSPDVARIIQATLDQPVPEHAYRLALEQRRTMQALYRDCFRSQG-VDALIFPTT 378 Query: 356 --YPRPVTLEE---LREQPVQRNSDLGWYTNFMNMLDYAAVATPMNFMKNGLPWGITVFG 410 RP+ ++ LR + V + ++ +++ P+ + GLP G+ + G Sbjct: 379 ALTARPLGEDDTVALRGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDG 438 Query: 411 PAFSDQRLLGVADALQ 426 P SD+RLL VA +L+ Sbjct: 439 PEGSDRRLLQVAASLE 454 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 102 bits (254), Expect = 6e-23, Method: Compositional matrix adjust. Identities = 113/395 (28%), Positives = 172/395 (43%), Gaps = 29/395 (7%) Query: 44 PLYGVPFSIKDNIDLAGIPTTAACPAFSYVPRESA--TLVAQLIALGAIPIGKCNLDQFA 101 PL GVP S+KD D+AG T A P SA +V L+ GAI IGK N+ +FA Sbjct: 68 PLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFA 127 Query: 102 TGLNGTRSPYGACRNSVHPDYPSG----GSSSGSSLAVALGLSSFALGTDTAGSGRVPAA 157 G YG +N+ + G GSSSG++++V G++ A+G+DT GS R+P+A Sbjct: 128 YSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSA 187 Query: 158 LNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQAMQPDERDEYSR 217 LN L G K T +S GV+P LD + + C M E Sbjct: 188 LNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVSVQ--------CCAIMDAVLAGEDYV 239 Query: 218 ANLPHNDAAAFQPPRKGLRVGVPRQLEFLGCSES-PSLFVEALGQLEAIGGVSVSIDFSP 276 A L H P +GLR+ P + G ++ + ALG+L A G + V + Sbjct: 240 AVLAH--------PLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPA 291 Query: 277 FLEAARLLYEGPWV-AERYSTIEALLHEKPEEILPVIRDVLRKAPQFSAVDTFRAQYRLQ 335 F A + +G + AE Y+ AL+ E + + + SA D + Sbjct: 292 FNALAAINAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARP 351 Query: 336 QLKAECDRILATVDCVVTPAY----PRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAV 391 + A+ + LA D +V P PR L+ + N + +N LD A+ Sbjct: 352 RWIAQVEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCAL 411 Query: 392 ATPMNFMKNGLPWGITVFGPAFSDQRLLGVADALQ 426 + P + P G+++ D+RLL + A++ Sbjct: 412 SLPCH-APGSAPVGLSLAACGGQDKRLLSIGLAVE 445 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 90.9 bits (224), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 107/402 (26%), Positives = 182/402 (45%), Gaps = 30/402 (7%) Query: 44 PLYGVPFSIKDNIDLAGIPTTAACPAFSYVPRES-ATLVAQLIALGAIPIGKCNLDQFAT 102 PL+G+P +KDN D +PTT A + + ++ A V +L GA+ +GK + + A Sbjct: 118 PLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVILGKTTMHELAA 177 Query: 103 GLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVPAALNNLV 162 G+ S G RN P GGSS G+ AVA ++ +G+DT GS R+PAA NL Sbjct: 178 GVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGSIRIPAAHQNLF 237 Query: 163 GLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQAMQPDERDEYSRANLPH 222 GL+ T+GL S +GV+P T D + + + +L + D + ++P Sbjct: 238 GLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDSSTVDANGHIPK 297 Query: 223 NDAAAFQPPR-KGLRVGVPRQLEFLGC----SESPSLFVEALGQLEAIGGVSVSIDFSPF 277 + + +G R+GV R L G +E + +AL QL+ G + + P Sbjct: 298 SYRDGLRADSLQGARIGVLRAL--FGAAPEDAEVSAAINKALQQLKDQGAIVTDVTI-PE 354 Query: 278 LEAARLLYEGPWVA--ERYSTIEALLHEKPEEILPVIRDVLRKAPQFSAVDT---FRAQY 332 L+ L G + E + A L P + + ++L + + ++ R Sbjct: 355 LDG---LLSGSSIIPYEFKYDLGAYLQSHPGAPVGSLGEILARGMEHELLEAGLRLRNSV 411 Query: 333 RLQ--QLKAECDRIL---ATVDCVVTPAYPRPVTLEELREQPVQRNSDL-----GWYTN- 381 LQ + K E ++++ + + ++T + L+ L +QR + L G N Sbjct: 412 DLQNPKDKEELEKVMLKRSALKSLMTDVMQKN-HLDTLVYPTIQRKAALIGEPQGGAMNC 470 Query: 382 -FMNMLDYAAVATPMNFMKNGLPWGITVFGPAFSDQRLLGVA 422 A+A P+ F ++GLP + + P F++ LLG+A Sbjct: 471 QLSATTGLPALALPVGFTEDGLPVSLELLAPEFAEPALLGLA 512 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,428,802 Number of extensions: 57308 Number of successful extensions: 182 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 170 Number of HSP's successfully gapped: 10 Length of query: 477 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 388 Effective length of database: 1,198,455 Effective search space: 465000540 Effective search space used: 465000540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)