BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0103.p (800 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 1468 0.0 gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2... 179 7e-46 gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2... 134 3e-32 gi|300313400|ref|YP_003777492.1| allophanate hydrolase subunit 1... 82 2e-16 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 1468 bits (3800), Expect = 0.0, Method: Compositional matrix adjust. Identities = 726/771 (94%), Positives = 746/771 (96%) Query: 1 LRIDTWVEAGCEIPPYFDPMIAKLISWQPTREAARVALNSALRETALYGVETNQHYLRQI 60 LRIDTWVEAGCEI PYFDPMIAKLISWQPTREAAR+AL++ALR T LYGVETNQHYLRQI Sbjct: 370 LRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVETNQHYLRQI 429 Query: 61 LDDVPFASGQPWTRCLEHLVYRADTVEVLSPGTQTTVQDHPGRIGYWAVGVPPSGPMDER 120 L DVPFASGQPWTRCLE LVYRADTVEVL+PGTQTTVQDHPGRIGYWAVGVPPSGPMDER Sbjct: 430 LADVPFASGQPWTRCLEQLVYRADTVEVLAPGTQTTVQDHPGRIGYWAVGVPPSGPMDER 489 Query: 121 ALRLGNRLLGNAEDAAGLEVTMMGPVLRFNTAASVVVTGAVIPVLLDGVAQPMATVLHIA 180 ALRLGNRLLGNAE+AAGLEVTM GP+LRFNTAA VVVTGAVIPVLLDGVAQPMATVLHIA Sbjct: 490 ALRLGNRLLGNAEEAAGLEVTMSGPILRFNTAAMVVVTGAVIPVLLDGVAQPMATVLHIA 549 Query: 181 AGSTLKLGTISGAGARSYLAVRGGVQVPAYLGSRSTFTLGQFGGHAGRALASGDILHLLP 240 AGSTLKLG ISGAGARSYLAVRGGVQVP YLGSRSTFTLGQFGGHAGRALASGDILHL P Sbjct: 550 AGSTLKLGAISGAGARSYLAVRGGVQVPTYLGSRSTFTLGQFGGHAGRALASGDILHLAP 609 Query: 241 LAGDEMPAAGQVLPADLYPALETVRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSS 300 LA D M AGQ LPA LYPALE VRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSS Sbjct: 610 LAADAMTQAGQTLPAALYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSS 669 Query: 301 RTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVC 360 RTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVC Sbjct: 670 RTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGFVC 729 Query: 361 PVTVIEADLWQIGQLKAGDKVRFIPVDVSTARRIALAAQAGIASLQAQPVEWSPAPLASP 420 PVTVIEADLWQIGQLKAGDKVRF+PV+V+TARRIALAAQAG+ASLQ QPV+WSPAPLASP Sbjct: 730 PVTVIEADLWQIGQLKAGDKVRFVPVEVNTARRIALAAQAGVASLQPQPVDWSPAPLASP 789 Query: 421 IVLDLGQDEQRLVARLSGDANLLLEVGSPQLDLVLRFRVHALIAALEEQALPGVIDLTPG 480 IVLDLGQDE+RLVARLSGDANLLLEVGSPQLDLVLRFRVHALI ALEEQALPGVIDLTPG Sbjct: 790 IVLDLGQDERRLVARLSGDANLLLEVGSPQLDLVLRFRVHALITALEEQALPGVIDLTPG 849 Query: 481 IRSLQVHYQPEALPLPRLLETIAGLWDAVCARQNLTVSSRIVHLPLSWDDPACQLAIEKY 540 IRSLQVHYQPEALPLP LL+TIAGLWDAVCA +NLTVSSRIVHLPLSWDDPACQLAIEKY Sbjct: 850 IRSLQVHYQPEALPLPTLLQTIAGLWDAVCASENLTVSSRIVHLPLSWDDPACQLAIEKY 909 Query: 541 MTTVRKDAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGAPVATPLDPR 600 MTTVRKDAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGAPVATPLDPR Sbjct: 910 MTTVRKDAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGAPVATPLDPR 969 Query: 601 HRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRYREVAAFRGK 660 HRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRYREVAAFRGK Sbjct: 970 HRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRYREVAAFRGK 1029 Query: 661 PWLLRFFDQIRFYPVTADELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQREAEGIAAF 720 PWLLRFFDQIRFYPVTA+ELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQREA+GIAAF Sbjct: 1030 PWLLRFFDQIRFYPVTAEELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQREADGIAAF 1089 Query: 721 RQHQQAAFDAERQRWIASGQANYESEESSAVVQDHQPLQAGQLSVDAPIAG 771 RQHQQAAFDAERQRWIASGQANYESEESSA+ Q+ PLQ GQ++VDAPIAG Sbjct: 1090 RQHQQAAFDAERQRWIASGQANYESEESSAIAQEAAPLQDGQMAVDAPIAG 1140 >gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 325 Score = 179 bits (454), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 123/312 (39%), Positives = 166/312 (53%), Gaps = 13/312 (4%) Query: 85 TVEVLSPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEDAAGLEVTMMG 144 T++V PG T+VQD GR GY+ +G+PPSG +D+ ALR N L+GN + LE +++G Sbjct: 2 TIKVKQPGLATSVQDG-GREGYYHLGIPPSGALDQYALRSANLLVGNHASTSALECSLLG 60 Query: 145 PVLRFNTAASVVVTGAVIPVLLDGVAQPMATVLHIAAGSTLKLGTISGAGARSYLAVRGG 204 P L F + A V VTGA + +DG +P+ T + +G L AGARSYLA+ GG Sbjct: 61 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSF-DYPKAGARSYLAIAGG 119 Query: 205 VQVPAYLGSRSTFTLGQFGGHAGRALASGDILHLLPLAGDEMPAA--GQVLPADLYPALE 262 + VP L SRST+ LG GG GR LA D L L G P A G+ LP L P + Sbjct: 120 IDVPVVLESRSTYALGALGGWQGRKLAKDDELPL----GRASPKAIEGRTLPLSLVPVYD 175 Query: 263 TVRQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSSRTGVRLIGPKPEWV--RDSGGE 320 +RV+ G + T +E FF W V + R G R G + R+ Sbjct: 176 REATLRVVPGLY--IHRLTDEAVERFFDDTWTVGSEADRIGYRFKGGQAMQFRPREQPFG 233 Query: 321 AGLHPSNIHDNPYAVGAVDFTGDM-PVILGPDGPSLGGFVCPVTVIEADLWQIGQLKAGD 379 AG PSNI D Y +G++ G + P++L D S GG+ TVI ADL IGQL+ Sbjct: 234 AGSDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNH 293 Query: 380 KVRFIPVDVSTA 391 K +F+ V + A Sbjct: 294 KAQFVRVTLEQA 305 >gi|300311090|ref|YP_003775182.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 344 Score = 134 bits (337), Expect = 3e-32, Method: Compositional matrix adjust. Identities = 122/361 (33%), Positives = 171/361 (47%), Gaps = 50/361 (13%) Query: 86 VEVLSPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEDAAGLEVTMMGP 145 ++++ PG +VQD GR G+ +G+ P G ++ AL N L+GN DAAGLE+TM Sbjct: 2 IDIIQPGMLASVQDM-GRYGHRRLGIHPGGALNTLALASANLLVGNPVDAAGLEITMGVA 60 Query: 146 VLRFNTAASVVVTGAVIPVLLDGVAQPMATV--LHIAAGSTLKLGTISG--------AGA 195 LRF A + + G I LDG +P+A + + AGS LKL + Sbjct: 61 ELRFTRATRIALGGDDIGATLDG--KPIAACWSIPVRAGSILKLSLAAADSPEQAPRHSM 118 Query: 196 RSYLAVRGGVQVPAYLGSRSTFTLGQFGGHAGRALASGDILHL-----------LPLAGD 244 R+YLAV GG+ VP LGSRST FGGH GRAL GD L + P G Sbjct: 119 RTYLAVAGGIDVPLVLGSRSTDLKAGFGGHQGRALKRGDKLPIGDAQAAAAAVHGPAFGV 178 Query: 245 EMPA-AGQVLPADLYPALETVRQVRVIYGPHGAPEY--FTPAYMETFFATDWEVHFNSSR 301 P +G L AD + T+R + APE+ F+ + E ++ W V NS+R Sbjct: 179 RSPRWSGLALRADEHSQHVTLRVLP-------APEFDQFSKSAREALWSDTWRVTPNSNR 231 Query: 302 TGVRLIGPKPEWVRDSGGEA---GLHPSNIHDNPYAVGAVDFTGDMPVILGPDGPSLGGF 358 G RL G PE R G + G+ P I P P+IL D + GG+ Sbjct: 232 MGYRLEG--PELKRKQGRDMLSHGVVPGVIQVPPSG---------QPIILMGDAQTTGGY 280 Query: 359 VCPVTVIEADLWQIGQLKAGDKVRFIPVDVSTARRIALAAQAGIASLQAQ--PVEWSPAP 416 +I ADLW++ Q +R P +++ A A Q + S++A ++WS A Sbjct: 281 PKIGVIIGADLWKLAQAPLNATLRLEPCEMADALAAAEEQQRYLDSIRATVAALDWSRAT 340 Query: 417 L 417 L Sbjct: 341 L 341 >gi|300313400|ref|YP_003777492.1| allophanate hydrolase subunit 1 [Herbaspirillum seropedicae SmR1] Length = 299 Score = 81.6 bits (200), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 76/276 (27%), Positives = 128/276 (46%), Gaps = 13/276 (4%) Query: 436 LSGDANLLLEVGSPQLDLVLRFRVHALIAALEEQALPGVIDLTPGIRSLQVHYQPEALPL 495 ++GD +L EV S + L FR A+ A+E+ ALPGV+D+ S Q+ + P+ + Sbjct: 16 IAGDEHLFAEV-SESMSLEAFFRAMAITRAVEKLALPGVLDVCLANASFQIRFNPDLIHP 74 Query: 496 PRLLETIAGLWDAVCARQNLTVSSRIVHLPLSWDDPACQLAIEKYMTTVRKDAPWCPS-- 553 LLE + L + A + + +RI+ +P+ +DDP ++ +D PS Sbjct: 75 LALLEQLEALESSTTAERR--IDTRIIEVPVLYDDPWTNETQSRF-----RDRHQDPSST 127 Query: 554 NLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGAPVATPLDPRHR-LVTTKYNPAR 612 +L++ RIN + A + V +G V G P + + R L KY R Sbjct: 128 DLQYAARINGYDETQAFIAAHSGTPWFVSMVGFV-AGLPFMYQMVEQDRQLQVPKYLRPR 186 Query: 613 TWTAENSVGIGGAYLCIYGMEGPGGYQFVGRT-LQMWNRYREVAAFRGKPWLLRFFDQIR 671 T T + ++G GG + CIY + G GGYQ G T +++ + + R R D ++ Sbjct: 187 TDTPKLTLGHGGCFGCIYSVRGAGGYQMFGVTPAPIYDPQQGLPYLRESMVFFRAGDIVQ 246 Query: 672 FYPVTADELQTIRRDFPLGRFEVGIEETQLNLADYQ 707 F P+ + GRF++ I + +L +Q Sbjct: 247 FKPIDRTAYDAAIVEVEAGRFDLRIRAVRFDLDAFQ 282 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,750,137 Number of extensions: 127645 Number of successful extensions: 384 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 377 Number of HSP's successfully gapped: 4 Length of query: 800 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 707 Effective length of database: 1,179,515 Effective search space: 833917105 Effective search space used: 833917105 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)