BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0102.p (364 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 664 0.0 gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 282 2e-77 gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 280 1e-76 gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 270 1e-73 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 664 bits (1712), Expect = 0.0, Method: Compositional matrix adjust. Identities = 327/333 (98%), Positives = 331/333 (99%) Query: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY Sbjct: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR Sbjct: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 Query: 121 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDAF 180 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAEL DAF Sbjct: 121 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELADAF 180 Query: 181 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGQGEVIALGVRDCSVQRRNQKVLEE 240 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDG+GEVIALGVRDCSVQRRNQKVLEE Sbjct: 181 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEE 240 Query: 241 TPAPNLPEGMADALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT 300 TPAPNLP+GMA+ALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT Sbjct: 241 TPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT 300 Query: 301 EQVWGVDLVRWMIELAAGDLPPLAQLAANLQPK 333 EQVWGVDLVRWMIELAAGDLPPLAQLAA LQP+ Sbjct: 301 EQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPR 333 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 282 bits (722), Expect = 2e-77, Method: Compositional matrix adjust. Identities = 160/328 (48%), Positives = 218/328 (66%), Gaps = 2/328 (0%) Query: 5 LLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTYLAVD 64 LLIANRG IA RI+R + L +T V S+AD SL AD +G A ++YL VD Sbjct: 8 LLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYLNVD 67 Query: 65 KILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTARALAR 124 +L AARDSGA A+HPGYGFLSENAAFAEA AAG+ FVGP +R G K AR A Sbjct: 68 ALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARRTAA 127 Query: 125 EQGVPMLEGT-DLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDAFDSV 183 GVP++ G+ LE + ALA A +GYP+++K++AGGGG G+R+ R EL F Sbjct: 128 AAGVPVVPGSAGELEDLATALACADEIGYPLLIKASAGGGGRGIRMARDATELAREFPLA 187 Query: 184 RRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGQGEVIALGVRDCSVQRRNQKVLEETPA 243 + Q F A V++E++I RARH+EVQ+ GDG+ + L R+CS+QRR QKVLEE P+ Sbjct: 188 QAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEEAPS 246 Query: 244 PNLPEGMADALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVTEQV 303 P L ALC +A++LA+ ++YR AGT+E+++D +G+F+F+E+NTR+QVEH VTE + Sbjct: 247 PALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVTEMI 306 Query: 304 WGVDLVRWMIELAAGDLPPLAQLAANLQ 331 G+DLV+ M+ +A G+ L Q +Q Sbjct: 307 TGIDLVQAMLRIAGGEPLGLQQSDIRMQ 334 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 280 bits (717), Expect = 1e-76, Method: Compositional matrix adjust. Identities = 174/367 (47%), Positives = 228/367 (62%), Gaps = 14/367 (3%) Query: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MF +LIANRG IACR+ T R L + V VYS+AD + HV D ++ LG PA ++Y Sbjct: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 L +KI+AAA +GAQAIHPGYGFLSENA FA+AC AG+ F+GP + + G K A+ Sbjct: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAK 120 Query: 121 ALAREQGVPMLEGTDLLESIDDAL-AAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDA 179 AL + VP++ G E L + A R+GYPV+LK++AGGGG GMRV + A Sbjct: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA 180 Query: 180 FDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGQGEVIALGVRDCSVQRRNQKVLE 239 S +R +F D V +EKY+ R RH+E+QVF DG G + L RDCSVQRR+QKVLE Sbjct: 181 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE 240 Query: 240 ETPAPNLPEGMADALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGV 299 E PAP + E A+ AA+ A+AV Y AGTVEF+ + G FYF+E+NTRLQVEH V Sbjct: 241 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQ-DGSFYFMEMNTRLQVEHPV 299 Query: 300 TEQVWGVDLVRWMIELAAGDLPPLAQ---------LAANLQPKNPLFLKFNKVPALXTCM 350 TE + G+DLV W + +AAG PL Q + A + +NP + +PA+ T Sbjct: 300 TEMITGLDLVEWQLRVAAGQALPLKQEQLRIHGHAIEARIYAENP---EKGFLPAIGTLT 356 Query: 351 RKTRAAS 357 R AA+ Sbjct: 357 RMRSAAA 363 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 270 bits (691), Expect = 1e-73, Method: Compositional matrix adjust. Identities = 142/321 (44%), Positives = 208/321 (64%), Gaps = 4/321 (1%) Query: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MF+ +LIANRG IA RI R R L + V V+SEAD + +V AD ++ +G P+ +Y Sbjct: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 L + I++AA + AQAIHPGYGFLSENA FAE E +G F+GP +R+ G K +A+ Sbjct: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAK 120 Query: 121 ALAREQGVPMLEGTD--LLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGD 178 GVP + G+D L ++ + + A ++GYPV++K+ GGGG GMRV ++A L + Sbjct: 121 QAMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 Query: 179 AFDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGQGEVIALGVRDCSVQRRNQKVL 238 A + F + V++EKY+E RH+E+Q+ D + I LG RDCS+QRR+QKV+ Sbjct: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAIWLGERDCSMQRRHQKVI 240 Query: 239 EETPAPNLPEGMADALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHG 298 EE PAP +P + + + + + +NYR AGT EF+Y++ +FYF+E+NTR+QVEH Sbjct: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYEN--EEFYFIEMNTRVQVEHP 298 Query: 299 VTEQVWGVDLVRWMIELAAGD 319 VTE + GVD+V+ I +AAG+ Sbjct: 299 VTEMITGVDIVQEQIRIAAGE 319 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,025,549 Number of extensions: 40745 Number of successful extensions: 148 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 142 Number of HSP's successfully gapped: 4 Length of query: 364 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 278 Effective length of database: 1,212,660 Effective search space: 337119480 Effective search space used: 337119480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)