BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0077.p (262 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 114 7e-27 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 112 3e-26 gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 111 8e-26 gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reduc... 105 5e-24 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 101 8e-23 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 114 bits (285), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 84/261 (32%), Positives = 135/261 (51%), Gaps = 25/261 (9%) Query: 2 KDLEGKVAVVTGGATLIGAGVVRRLRSYGVKVALFDIDADGGQR-----VAGEDRSACRF 56 D +VA+VTGGA IGA + ++L + G V + DI+ D + VA R+A Sbjct: 4 NDSGARVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAP-- 61 Query: 57 WRVDITDDAQVAKAVEEVAAHFGRLDFLVNLAA-----SYLDDGAASSRQDWLKTLDVNL 111 ++D+ +A A + +GR D LVN A S+LD ++WL+T++VN+ Sbjct: 62 LQMDLGSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLD----YPLENWLQTMNVNV 117 Query: 112 VSAVMAAHAVRPYLVKAGGGAIVNFTSISSKVAQTGRWLYPVSKAALVQVTRNMAMDFAG 171 ++A +VK G IVN +SIS A GR Y SKAA++ +TR MA++ A Sbjct: 118 TGVLLAGQHAARLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQ 177 Query: 172 DAIRVNSVSPGWTWSRVMDELTGGDRAKTDRVAADFHLL---KRVGNPEEVADVVAFLLS 228 I VNSV+PG + + + +++ ++ L +R G +E+A V +L + Sbjct: 178 YGITVNSVAPGPVDTPMTQAM------HSEQTRESYYRLVPMRRYGTTDEIASAVCYLAA 231 Query: 229 GHASFVTGADYAVDGGYSAMG 249 ++++TG VDGG+ A G Sbjct: 232 EESAYITGHVIPVDGGFVAGG 252 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 112 bits (280), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 85/251 (33%), Positives = 121/251 (48%), Gaps = 9/251 (3%) Query: 1 MKDLEGKVAVVTGGATLIGAGVVRRLRSYGVKVALFDIDADGGQRV---AGEDRSACRFW 57 ++ EGKV +VTG A+ IG RR G +V L D DA +V +R+A R Sbjct: 9 LQRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAAR-- 66 Query: 58 RVDITDDAQVAKAVEEVAAHFGRLDFLVNLAASYLDDGAAS-SRQDWLKTLDVNLVSAVM 116 D++ QV + V+ FG+LD LV+ A + + S +DW + N+ Sbjct: 67 ETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFY 126 Query: 117 AAHAVRPYLVKAGGGAIVNFTSISSKVAQTGRWLYPVSKAALVQVTRNMAMDFAGDAIRV 176 A P+L K G IVN S+S A Y SK A+ +TR MA+DF +R+ Sbjct: 127 GAREALPHLEKTRG-CIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRI 185 Query: 177 NSVSPGWTWSRVMDELTGGDRAKTDRVAADFHLLKRVGNPEEVADVVAFLLSGHASFVTG 236 N+V P T + + ++ D D+ A L R +P E+A V+ FL S ASFV G Sbjct: 186 NAVCPSLTHTAMTADM-ADDPPLLDKFAERI-ALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 237 ADYAVDGGYSA 247 + VDGG A Sbjct: 244 VNLPVDGGLMA 254 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 111 bits (277), Expect = 8e-26, Method: Compositional matrix adjust. Identities = 83/253 (32%), Positives = 130/253 (51%), Gaps = 10/253 (3%) Query: 3 DLEGKVAVVTGGATLIGAGVVRRLRSYGVKVALFDIDADGGQ----RVAGEDRSACRFWR 58 +L+ KV +VTGGA+ IGA + +L + G +F A Q A + R+A ++ Sbjct: 4 NLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPESQFWERLTAVQPRAA--LFQ 61 Query: 59 VDITDDAQVAKAVEEVAAHFGRLDFLVNLAASYLDDGAASSRQDWLKTLDVNLVSAVMAA 118 +++ DDA+ +AV FGRLD LVN A G + R +++ +L+ NL+ + A Sbjct: 62 LELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVMA 121 Query: 119 HAVRPYLVKAGGGAIVNFTSISSKVAQTGRWLYPVSKAALVQVTRNMAMDFAGDAIRVNS 178 H P+L KA GAI+N +S ++ Q Y SK A + +TR A D +RVN+ Sbjct: 122 HYCVPHL-KATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 Query: 179 VSPGWTWSRVMDELTGG---DRAKTDRVAADFHLLKRVGNPEEVADVVAFLLSGHASFVT 235 + P + + ++ + K D + + L KR EE+AD+ FLLS +S T Sbjct: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 Query: 236 GADYAVDGGYSAM 248 G VDGGY+ + Sbjct: 241 GQWIFVDGGYTHL 253 >gi|300309917|ref|YP_003774009.1| short chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 105 bits (261), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 82/242 (33%), Positives = 122/242 (50%), Gaps = 11/242 (4%) Query: 4 LEGKVAVVTGGATLIGAGVVRRLRSYGVKVALFDIDADGGQRVAGEDRSACRFWRVDITD 63 L+GK A+VTGG IGA V R+L + G +V + + AG + + +++ Sbjct: 8 LKGKTALVTGGTQGIGAAVARQLVALGARVIAAGLPPFAHEEFAGSEVT---LVEMNVAS 64 Query: 64 DAQVAKAVEEVAAHFGRLDFLVNLAASYLDDGAASSRQDWLKTLDVNLVSAVMAAHAVRP 123 + V A+ A +LD +VN A + GA + + + + VNL + A R Sbjct: 65 EESVRAAL----AGLTQLDIVVN-CAGIIKRGAELETEVFEQVIAVNLTGTMRVCAAAR- 118 Query: 124 YLVKAGGGAIVNFTSISSKVAQTGRWLYPVSKAALVQVTRNMAMDFAGDAIRVNSVSPGW 183 L++ G IVN S+ S Y SK + Q+T+++A+ +A D IRVN+V+PGW Sbjct: 119 ELLRQSRGCIVNTASMLSFFGGGLVPGYSASKGGVAQLTKSLAIAYAADGIRVNAVAPGW 178 Query: 184 TWSRVMDELTGGDRAKTDRVAADFHLLKRVGNPEEVADVVAFLLSGHASFVTGADYAVDG 243 + + L D + + A L R G PE+V VVAFL S ASF+TGA VDG Sbjct: 179 IATPLTQALQD-DPVRAGPILARTPL-GRWGQPEDVGQVVAFLCSPAASFMTGAIVPVDG 236 Query: 244 GY 245 GY Sbjct: 237 GY 238 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 101 bits (251), Expect = 8e-23, Method: Compositional matrix adjust. Identities = 92/266 (34%), Positives = 124/266 (46%), Gaps = 36/266 (13%) Query: 1 MKDLEGKVAVVTGGATLIGAGVVRRLRSYGVKVALFD------------IDADGGQR--V 46 M L+GKVA++TG + IG +G + L I A GG+ V Sbjct: 1 MNTLQGKVALITGASAGIGRATALLFARHGAALILTARRQDALEQVAALICAQGGRASVV 60 Query: 47 AGEDRSACRFWRVDITDDAQVAKAVEEVAAHFGRLDFLVNLAASY--LDDGAASSRQDWL 104 AG+ A T + VA A++E FG LD +N A + A S Q W Sbjct: 61 AGDVGEAA-------THERGVAMALDE----FGGLDIAINNAGAVGACKPLAEISPQQWQ 109 Query: 105 KTLDVNLVSAVMAAHAVRPYLVKAGGGAIVNFTS--ISSKVAQTGRWLYPVSKAALVQVT 162 TL NL SA + A P ++ G GAIV FTS + S G Y +KA L+ + Sbjct: 110 DTLHANLTSAFLGARCQIPAMLARGAGAIV-FTSSFVGSSAGLPGMAAYGAAKAGLMGLV 168 Query: 163 RNMAMDFAGDAIRVNSVSPGWTWSRVMDELTGGDRAKTDRVAADFHLLKRVGNPEEVADV 222 + +A D+A IR N++ PG +D GGD + + AA H +KR+ PEE+A Sbjct: 169 KGIAADYAVQGIRANALLPGG-----VDTDMGGDP-QQKQWAAGLHAMKRIAQPEEIAAA 222 Query: 223 VAFLLSGHASFVTGADYAVDGGYSAM 248 FL S ASFVTG DGG SA+ Sbjct: 223 ALFLASPMASFVTGTALYADGGNSAV 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 843,115 Number of extensions: 33455 Number of successful extensions: 279 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 46 Length of query: 262 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 179 Effective length of database: 1,226,865 Effective search space: 219608835 Effective search space used: 219608835 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)