BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0076.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway... 127 9e-31 gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003... 87 1e-18 gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway... 83 3e-17 >gi|300312311|ref|YP_003776403.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 127 bits (320), Expect = 9e-31, Method: Compositional matrix adjust. Identities = 91/294 (30%), Positives = 141/294 (47%), Gaps = 15/294 (5%) Query: 22 ALACLPAHAIDGSGLPSYPDGLENFGSGALPPPGVYGMVYTGFAQYD--HIRGNNGQDIG 79 A LPA A++ G P G+ +FG+G LPP +G V + Y ++ G+ I Sbjct: 26 AFFSLPAQAVE-QGATITPIGITDFGAGMLPPSSPFGTVGVRASYYSASTLKDGAGKSI- 83 Query: 80 PPDFRVQVGVLAPRVVWVTQQSILGGQLAFHALAPLLDVD------VRAGGRNFHSR--G 131 P DF + V LA ++T +LG + + P +D+ AG + R G Sbjct: 84 PNDFHIDVKTLALAYFYMTDVEVLGARFGVGGVLPFIDISGGLKVGTPAGPLSVSGRDSG 143 Query: 132 LGDITVGP-ALAYHASPALHYAVGFDITMPSGQYSADNPSSLGKNIWVYQPLVALSYIQR 190 LGD+ V P LA+ P++ G I P+G YS + G N W Y P V +YI Sbjct: 144 LGDMQVLPFILAWQLPPSVFVNAGLMIQAPTGAYSTSKAFNAGVNHWTYSPFVGATYITS 203 Query: 191 EGVNADVKLMYDFNGRNGATQTRSGQAIHADYALGWGFGNGLVAGVGGYVYQQVTNDSGP 250 G+ ++ + N N AT+ RSG ++A+G AG+ GYVY+Q+++DSGP Sbjct: 204 SGLELSTQVSLNVNTVNPATRYRSGVEYRQEFAIGQHI-QSWTAGLAGYVYRQLSDDSGP 262 Query: 251 NAAAG-KSQALAIGPNLKYDNGKGLVVTAKFQQEYDVRNRPEGKQFYVKAILPF 303 + G + + A+GP + + +VT QE+ RNR EG ++ + F Sbjct: 263 GSGDGNRGRVYALGPAVSFAQPGLPLVTLHGYQEFGARNRAEGYNLALRLAMTF 316 >gi|300310336|ref|YP_003774428.1| hypothetical protein Hsero_1003 [Herbaspirillum seropedicae SmR1] Length = 305 Score = 87.4 bits (215), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 71/292 (24%), Positives = 134/292 (45%), Gaps = 11/292 (3%) Query: 17 CSLGLALACLPAHAIDGSGLPSYPDGLENFGSGALPP-PGVYGMVYTGFAQYDHIRGNNG 75 C+L A A+A +G G+ + G + ALPP PG++ + ++ + + NNG Sbjct: 14 CALLTMAAGPAAYAFEG-GVSPFLTGSTSEYIAALPPIPGLFAVEQFNYSSSNGLYDNNG 72 Query: 76 QDIGPPDFRVQVGVLAPRVVWVTQQSILGGQLAFHALAPLLDVDVRAGGRNFHSRGLGDI 135 + P F+ R++ LG ++ + P++ + G + GL ++ Sbjct: 73 NKL-PIPFKSSAYSATTRLLASYGVQWLGAKVYSQLVLPVVSLHTEVAGHSETHNGLSNV 131 Query: 136 TVGPAL-AYHASPALHYAVGFDITMPSGQYSADNPS-SLGKNIWVYQPLVALSYIQREGV 193 T P L + + + GFDI++ +G YS PS ++G W QP++A+ + +G+ Sbjct: 132 TATPVLLRWDVAAHTNVTFGFDISLETGSYSPTRPSVAVGYTSW--QPVLAVRHNNPDGL 189 Query: 194 NADVKLMYDFNGRNGATQTRSGQAIHADYALGWGFGNGLVAGVGGYVYQQVTND--SGPN 251 + + N RN +T RSG A AD+ GW G G Y+ Q T+D +G + Sbjct: 190 DLGISNRLLINDRNSSTNYRSGTAYVADFIGGWNVGKWKFGVTGSYL-NQFTDDRQNGAD 248 Query: 252 AAAGKSQALAIGPNLKYDNGKGLVVTAKFQQEYDVRNRPEGKQFYVKAILPF 303 ++++L++GP + Y+ G + +Q+ N + ++ +P Sbjct: 249 ITGNRARSLSLGPTVAYNAGP-FSINMNYQRGLYAANTAKSDAIWINFAMPL 299 >gi|300313864|ref|YP_003777956.1| enzyme involved in meta-pathway of phenol degradation protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 83.2 bits (204), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 77/287 (26%), Positives = 130/287 (45%), Gaps = 26/287 (9%) Query: 37 PSYPDGLENFGSGALPP--PGVYGMVYTGFAQYDHIRGNNGQDIGPPDFRVQVGVLAP-- 92 P+ G +F GA P PG Y + Y +++ + NNG +G P + + V AP Sbjct: 26 PTVNMGGTSFFDGAPLPGGPGFYFVEYLTYSKATKLVDNNGNRLGFPATQ-DISVFAPVT 84 Query: 93 RVVWVTQQSILGG-QLAFHALAPLL-DVDVRAGGRNFHSRG---LGDITVGPALAYHASP 147 + ++V + G QL F L P + V G N RG +GDI+ G ++ Y Sbjct: 85 QFIYVPPGAKFGNKQLGFQVLVPWVASAKVDDGANNAVLRGNTGIGDISAGVSVQYDPIM 144 Query: 148 ALH---YAVGFDI--TMPSGQYSADNPSSLGKNIWVYQPLVALSYIQREGVNADVKLMYD 202 YA F++ +P+G Y + + G N W + P A + + ++ +L Y Sbjct: 145 GAQGPLYAQRFELQFILPTGAYDRNATVNPGSNTWAFDPYWAGTLWASQNLSFSWRLHYL 204 Query: 203 FNGRNGA---------TQTRSGQAIHADYALGWGFGNGLVAGVGGYVYQQVTND--SGPN 251 +N RN A + T++GQA HA++A + G GY QQVT+ +G + Sbjct: 205 WNARNNAPSAAFGNNVSSTQAGQAAHANFAAEYVLNPTWTVGAAGYWLQQVTDTKVNGAD 264 Query: 252 AAAGKSQALAIGPNLKYDNGKGLVVTAKFQQEYDVRNRPEGKQFYVK 298 + + + + +GP + ++ QE+ VRNR E +F ++ Sbjct: 265 VSGRRERVVGLGPAAMMRLSERDLLFFNVYQEFAVRNRAENSKFQIR 311 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.139 0.429 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,008,342 Number of extensions: 46306 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 93 Number of HSP's successfully gapped: 3 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)