BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0065.p (674 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313770|ref|YP_003777862.1| TonB-dependent siderophore rece... 1226 0.0 gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitr... 66 9e-12 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 65 1e-11 gi|300312552|ref|YP_003776644.1| TonB-dependent outer membrane i... 65 1e-11 gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor prote... 61 2e-10 >gi|300313770|ref|YP_003777862.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 1226 bits (3171), Expect = 0.0, Method: Compositional matrix adjust. Identities = 585/674 (86%), Positives = 635/674 (94%) Query: 1 VFKLTALSGLLALGTAPWALAQSASAAEDSSLTLGTVEVSARRSGPLQLNNVLTSVDLIG 60 + + TAL GLLA P ALAQS +++ED++LTLGTVEVS RRSGPLQL NVLTSVDLIG Sbjct: 23 LVRATALGGLLAFSAVPDALAQSTASSEDAALTLGTVEVSGRRSGPLQLRNVLTSVDLIG 82 Query: 61 ADRIQQQQAQYNWELFAQSPGVMLTQFRQGNESGKLSFRGFNGEGEVNAVKLLIDGIPSN 120 ADR+QQQQ YNWELFAQSPGVMLTQFRQGNESGKLSFRGFNGEGEVNAVKLLIDG+PSN Sbjct: 83 ADRLQQQQVLYNWELFAQSPGVMLTQFRQGNESGKLSFRGFNGEGEVNAVKLLIDGVPSN 142 Query: 121 DNAGGMPFLGTLFPLDIARIEVVRGTNDARYGLHNIAGNVNIVTRQGGNYDEARLTYGSF 180 DNAGGMPFLGTLFPLD+A IEVVRGTNDARYGLHNIAGNVNI+TRQGGNYDEARLTYGSF Sbjct: 143 DNAGGMPFLGTLFPLDMAGIEVVRGTNDARYGLHNIAGNVNILTRQGGNYDEARLTYGSF 202 Query: 181 NTRQVQVVKGVENGNWSQNYFVGYEKSDGYRDHAQAENIAFSGKWFYTSDDGRYRTGLSI 240 NTRQVQV KGVENGNWSQNYF+GYEKSDGYR+HAQAENIAFSGKWFYTSDDGRYRTGLSI Sbjct: 203 NTRQVQVAKGVENGNWSQNYFLGYEKSDGYREHAQAENIAFSGKWFYTSDDGRYRTGLSI 262 Query: 241 RHANAYAQEPGYLTAQDAHSNPTSSYAYAQSDGGNRQMDQVSLHFDGDIDDQLSVGAKLY 300 RHANAYAQEPGYLTA+DA +NP +SYAYAQSDGGNRQ+DQVSLHFDGDIDDQL+VGAKLY Sbjct: 263 RHANAYAQEPGYLTAEDARANPRTSYAYAQSDGGNRQLDQVSLHFDGDIDDQLAVGAKLY 322 Query: 301 LNRVRDQRWLRYSSTTSQQERVIDETHYGLLTNATYRPRWKALEGMSDLALEGGFSMERQ 360 LNRVRDQRWLRYSSTTSQQER+IDETHYGL+T ATYRP+WKALEGM+DLALEGGF++ERQ Sbjct: 323 LNRVRDQRWLRYSSTTSQQERLIDETHYGLMTTATYRPQWKALEGMNDLALEGGFNVERQ 382 Query: 361 EDQSPRYNTVNQVRVSQTRDHHFVFDTVGAYVQAVIKPVESLRIIPGYRVDHVGGRFNDP 420 EDQSPR+NTVN+VRV+QTRDHHF FDT GAY+QAVIKPVES +IIPGYRVD VGG F+DP Sbjct: 383 EDQSPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVIKPVESFKIIPGYRVDKVGGSFSDP 442 Query: 421 SRGVSYGIQDYGYIRQPKLSVVYAPIREASLYANWGRTFQVGAGAAAYKSNNNDLRPSIN 480 SRGV YGIQDYG IRQPKLSVVYAPI++ASLYANWGRTFQVGAGAAAYKSNNNDLRPSIN Sbjct: 443 SRGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWGRTFQVGAGAAAYKSNNNDLRPSIN 502 Query: 481 DGWETGVKFTPVDWLDGRVAVWEQRASDEVKRRLNDPTGDSENVGRTQRKGVDLQINLRA 540 DGWE+GVKFTPV+WLDGR+A WEQRASDEVKRRLNDPTGDSENVGRT+R GVDLQ+NLRA Sbjct: 503 DGWESGVKFTPVEWLDGRLAWWEQRASDEVKRRLNDPTGDSENVGRTKRSGVDLQLNLRA 562 Query: 541 SKRTTLWLAYSRQRSEIITPDPTAPNTLGKEIDHVPRQVYSGGIDYQATADLKLSASVNG 600 KRTT+WLAYSRQRSEIITPDPTAP T+GKEIDHVPR ++SGG+DYQAT LKLS S+NG Sbjct: 563 GKRTTMWLAYSRQRSEIITPDPTAPATIGKEIDHVPRYLFSGGVDYQATDYLKLSTSLNG 622 Query: 601 QGSYYLTTANTGGKYGGYTLFNASATYQVNDKLSLELQLKNLANRYYEYVWINDQTRHAP 660 QGSYYLTTANTGGK+GGYTL +ASA+YQV+ L+LELQ+KNLANRYYEYVWINDQTRHAP Sbjct: 623 QGSYYLTTANTGGKFGGYTLVDASASYQVSPTLALELQVKNLANRYYEYVWINDQTRHAP 682 Query: 661 GDGRAVYLSANVKY 674 GDGRA YLSA + Y Sbjct: 683 GDGRAAYLSAKLTY 696 >gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitrate transport protein [Herbaspirillum seropedicae SmR1] Length = 703 Score = 66.2 bits (160), Expect = 9e-12, Method: Compositional matrix adjust. Identities = 151/709 (21%), Positives = 270/709 (38%), Gaps = 63/709 (8%) Query: 5 TALSGLLALGTAPWALAQSASAAEDSSLTLGTVEVSARRSGPLQLNNVLT---SVDLIGA 61 TAL+ L L LAQ AS + D L ++VS G ++V T + ++ Sbjct: 19 TALASLAILAFPVAGLAQQASPS-DGQPGLEPIQVSGDWLGTGLESSVKTYPGARTVVKK 77 Query: 62 DRIQQQQAQYNWELFAQSPGVMLTQFRQGNESGKLSF----RGFNGEGEVNAVKLLIDGI 117 I+ A + + PGV + G ++ RG G + +L+DGI Sbjct: 78 QEIESTGAVSIGDAMRRIPGVQSSD-NSGTAGSAIALNIGVRGLAGRYSPRST-ILLDGI 135 Query: 118 PSNDNAGGMPFLGTLFPL---DIARIEVVRGTNDARYGLHNIAGNVNIVTRQ----GGNY 170 P G P L + PL +I I+VVRG R+G N+ G +N TR G Sbjct: 136 PMAVAPYGQPQL-SFAPLSLNNIESIDVVRGGGAVRFGPQNVGGIINFKTRSIPTTPGIS 194 Query: 171 DEARLTYGSF-----NTRQVQVVKGVENGNWSQNYFVGYEKSDGYRDHA--QAENIAFSG 223 +A + Y S+ NT+ G + + ++ G+RD + + A Sbjct: 195 GDAAVRYNSYGQGGGNTQYTAFAGGQAENGFGMAFLYSGQEGSGWRDKSDEHLNDFALKM 254 Query: 224 KWFYTSDDGRYRTGLSIRHANAYAQEPGYLTAQDAHSNPTSSYAYAQSDGGNRQMDQVSL 283 ++ + Y I + + ++ PG L+ ++P S + GG R+ V Sbjct: 255 RYEISPTSELY---AKINYYDVLSRTPGGLSVAQYAADPFQSTRQRDNWGGTRKGFDVG- 310 Query: 284 HFDGDIDDQLSVGAKLYLNRVRDQRWLRYSSTTSQQERVIDETHYGLLTNATYRPRWKAL 343 + I D+ + Y ++ Q L + T + + G+ T R W Sbjct: 311 -YLNTISDRQEFEIRTYFSQATRQSTLINPAGTRLSHQPRNYETLGIEPRYTQRFAWGGT 369 Query: 344 EGMSDLALEGGFSMERQEDQSPRYNTVNQVRVSQTRDHHFVFDTVGAYVQAVIKPVESLR 403 D+ + + ER +D + V T+ + D Y+ I R Sbjct: 370 --THDVTVGYRYVRERGDDNVYSEAVLTGVLSGFTQFQNST-DAHAGYIDDKIA-FGKWR 425 Query: 404 IIPGYRVDHVGGRFNDPSRGVSYGIQDYGYIRQPKLSVVYAPIREASLYANWGRTFQVGA 463 I PG R +HV D + SY + + + P ++V Y + +L++N+ +F V Sbjct: 426 ITPGLRFEHVQSTRYDVANNASYQVSNNKPL--PSINVAYLLTEQLTLFSNYNTSFGVVQ 483 Query: 464 GAAA-YKSNNNDLRPSINDGWETGVKFTPVDWLDGRVAVWEQRASDEVKRRLNDPTGDS- 521 + S+ N L P + E GV+ W R + + ++ G + Sbjct: 484 NSQLNTMSSANPLSPELAKTIEGGVR-----WKSARASAEATVFHIDFDNQIVSVQGTNP 538 Query: 522 ---ENVGRTQRKGVDLQINLRASKRTTL--WLAYSR-QRSEIITPDPTAPNTLGKEIDHV 575 N+G+TQ G++L ++ +++ L W Y+ + + P GK++ Sbjct: 539 SVFRNIGKTQHDGIELAMDYAFDQQSVLKGWSVYANYTYTRALQKSGANP---GKDLPFY 595 Query: 576 PRQVYSGGIDYQATADLKLSASVNGQGSYYLTTANT--------GGKYGGYTLFNASATY 627 R + G ++ + + S QG + ANT G+ G+ L+NA + Sbjct: 596 ARTTDTVGTRFK-SGPWGFNLSTTHQGRQFSDEANTIAESADGSIGEIPGFRLWNAQVDW 654 Query: 628 QVNDKLSLEL--QLKNLANRYYEYVWINDQTRHAPGDGRAVYLSANVKY 674 +V ++ + NL +R Y ++ G R VY+ + Sbjct: 655 KVPGTKGFDVFAGINNLTDRRYFTRTTDNNLGKLVGAPRTVYVQGRYSF 703 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 150/639 (23%), Positives = 249/639 (38%), Gaps = 101/639 (15%) Query: 98 FRGFNGEGEVNAVKLLIDGIPSNDNAGGMPFLGTLFPLDIARIEVVRGTNDARYGLHNIA 157 F F G +A LL+DG+ D+ + LG +I R+EV+RG YG + Sbjct: 118 FDAFRIRGFSSANDLLLDGM--RDDPQYVRGLG-----NIERVEVLRGPASVLYGRGSGG 170 Query: 158 GNVNIVTRQGGN--YDEARLTYGSFNTRQVQVVKGVENG-NWSQNYFVGYEKSDGYRDHA 214 G +N +++Q G +A L+ GS + +W+ G E + +RD Sbjct: 171 GVINRISKQPGQDVASQATLSLGSAGLLGAALDWNRPLAPHWALRINTGREHAGSFRDTV 230 Query: 215 QAENIAF-------SGK--WFYTSDDGRYR-------TGLSIRHAN----AYAQE----- 249 F SG+ W ++ Y G +I AN AYA Sbjct: 231 NGTRQYFAPALKWESGRDSWLLQAEYDEYERVPDRGIPGRAIATANGEPTAYALPSAGHE 290 Query: 250 -----PGYLTAQDAHSNPTSSYAYAQSDGGNRQ----MDQVSLHFDGDIDDQLSVGAKLY 300 G +D H + S+ A S + + ++ +FD Q A L Sbjct: 291 QFFGAAGRDVIRDIHMSLRSTLTRALSQDWTLRHALALMDLNSNFDNTFVTQAYQSAPLD 350 Query: 301 LNRVRDQRWLRYSSTTSQQERVIDETHYGLLTNATYRPRWKALEGMSDLALEGGFSMERQ 360 L+RV+ R+ + Q+R + + + LL G DL L S +++ Sbjct: 351 LDRVQRTRY-----QQNLQQRSV-QNNLELLGQL----------GAHDLLLGVEHSWQKR 394 Query: 361 EDQ--SPRYNTVNQVRVSQTRD----------HHFVFDTVGAYVQAVIKPVESLRIIPGY 408 E + S V+ V TR+ ++ +G YVQ I +++ G Sbjct: 395 EPRLWSVAAPAVSAVTPENTRNGDDPTTPFQMNYHKASNLGLYVQDKITLAAHWKMLAGL 454 Query: 409 RVDHVGGRFNDPSRGVSYGIQD--YGYIRQPKLSVVYAPIREASLYANWGRTFQVGAG-- 464 R D RF SR G++ + P++ VV+ + Y +W + F G Sbjct: 455 RWD----RFEVDSRNRRNGVRSERTAHALSPRIGVVWEALPGQHAYLSWSKNFAPVGGDV 510 Query: 465 ---AAAYKSNNNDLRPSINDGWETGVKFTPVDWLDGRVAV---WEQRASDEVKRRLNDPT 518 + N NDL+P + E G+K +WLD R++ W Q + R + DP Sbjct: 511 IGITPDARGNFNDLKPQFSRQVEAGIK---SEWLDRRLSTTLAWFQ--LELFNRNVADPV 565 Query: 519 --GDSENVGRTQRKGVDLQINLRASKRTTLWLAYSRQRSEIITPDPTAPNTLGKEIDHVP 576 G E G + +G++L + + + ++ Q ++I+ + P +GK V Sbjct: 566 RPGFFEQSGLERNRGIELSVEGELAAPWFVRAGWAIQSAKIVQAE---PKFVGKRASGVS 622 Query: 577 RQVYSGGIDYQATADLKLSASVNGQGSYYLTTANTGGKYGGYTLFNASATYQVNDKLSLE 636 + S + Y T + +G+ Y N + YT ++ Y++ D+ Sbjct: 623 GRGGSLFLSYAPTLGWFAELGLTVEGARYADRDNL-LELPAYTRWDGKMGYRL-DEAEFT 680 Query: 637 LQLKNLAN-RYYEYVWINDQTRHAPGDGRAVYLSANVKY 674 L + NLAN RYYE Q R PG RA+ L+A K+ Sbjct: 681 LAVTNLANRRYYESATGLSQVR--PGAPRALLLTAAYKF 717 >gi|300312552|ref|YP_003776644.1| TonB-dependent outer membrane iron monomeric catechols receptor protein [Herbaspirillum seropedicae SmR1] Length = 799 Score = 65.5 bits (158), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 83/328 (25%), Positives = 137/328 (41%), Gaps = 52/328 (15%) Query: 386 DTVGAYVQAVIKPVESLRIIPGYRVDHVGGRFNDPSRGVSYG------------------ 427 DTV AY+ IK + G RVDH + R VS G Sbjct: 485 DTVSAYLFDTIKVNQQWSFNGGVRVDHYDTSLANGYRCVSSGRGTLCPTGVAAGTAFRQS 544 Query: 428 IQDYGYIRQPKLSVVYAPIREASLYANWGRTFQVGAGAAAYK------SNNNDLRPSIND 481 G + KL+ +Y P +SLYA + + Q G++ ++N P Sbjct: 545 FSTSGNLLNWKLAGIYKPTDYSSLYALYATSQQPPGGSSFSLSASNSSADNPKFDPQKAK 604 Query: 482 GWETGVKFTPVDWLDGRVAV----WEQRASDEVKRRLNDPTGDSENVGRTQRKGVDLQIN 537 E G K+ D L+ ++A+ + ++EV++ + +G G+ +GV+L + Sbjct: 605 TAELGTKW---DLLNKKLALTAALYRTEITNEVEQ--DATSGQYFQTGKKTVQGVELGVT 659 Query: 538 LRASKRTTLWLAYSRQRSEIITPDPTAPNTLGKE--IDHVPRQVYSGGIDYQATADLKLS 595 + +L Y+ ++++ D A T E + + PRQ ++ Y+ L Sbjct: 660 GEVTPDLSLTAGYTYMKTKV---DSGAIVTASGENTLAYTPRQAFTSWATYRLPKGFTL- 715 Query: 596 ASVNGQGSYYLTTANTGG--------KYG-GYTLFNASATYQVNDKLSLELQLKNLANRY 646 G G+ Y+++ G KY Y +F+A A Y VN L L+L L N+ N+ Sbjct: 716 ----GGGARYVSSLVRGSDNPTSGTPKYADSYWVFDAMAGYVVNKNLDLQLNLYNIFNKD 771 Query: 647 YEYVWINDQTRHAPGDGRAVYLSANVKY 674 Y R+ PG RAV L+AN+K+ Sbjct: 772 YVAAINKSGYRYTPGTPRAVTLTANLKF 799 >gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 755 Score = 61.2 bits (147), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 71/303 (23%), Positives = 121/303 (39%), Gaps = 20/303 (6%) Query: 386 DTVGAYVQAVIKPVESLRIIPGYRVDHVGGRFNDPSRGVSYGIQDYGYIRQPKLSVVYAP 445 DTV Y +K E + G R D F+D + G + K++VV+ P Sbjct: 459 DTVSLYALDTLKFNERWSLSLGLREDFYRTSFDDTKPNAGGPLHKSGGLFNAKVAVVFKP 518 Query: 446 IREASLYANWGRTFQVGAGA----AAYKSN--NNDLRPSINDGWETGVKFTPVD-WLDGR 498 +SLYA + Q GA +A +SN + D P D E G K+ +D L Sbjct: 519 TSYSSLYALYASAQQPPGGANFNLSASRSNASHTDYAPQKTDTGEIGTKWELLDRKLIAT 578 Query: 499 VAVWEQRASDEVKRRLNDPTGDSENVGRTQRKGVDLQINLRASKRTTLWLAYSRQRSEI- 557 A++ ++EV+ L+ T G+ +G++L + S +L YS +S++ Sbjct: 579 AALYRTEVNNEVE--LDSLTNTYFQTGKKITQGLELGVTGDLSPTVSLTAGYSHMQSQVS 636 Query: 558 --ITPDPTAPNTLGKEIDHVPRQVYSGGIDYQATADLKLSASVNGQGSYY----LTTANT 611 T N L + PR+ ++ + ++ L + G T Sbjct: 637 RGALVSATGENFLA----YAPRRSFTSWVSWRLPQGFTLGGGLRYSGGLLRGRDTNAIGT 692 Query: 612 GGKYGGYTLFNASATYQVNDKLSLELQLKNLANRYYEYVWINDQTRHAPGDGRAVYLSAN 671 G Y + + A+Y ++ ++L + NL N Y R+ PGD R + L+ N Sbjct: 693 PKDTGAYWVADVMASYVIHQHAEIQLNVNNLFNNSYVRSINKSGYRYLPGDARNISLTGN 752 Query: 672 VKY 674 + Sbjct: 753 FHF 755 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,146,666 Number of extensions: 93767 Number of successful extensions: 276 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 8 Length of query: 674 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 582 Effective length of database: 1,184,250 Effective search space: 689233500 Effective search space used: 689233500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)