BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0055.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogen... 662 0.0 gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspi... 170 2e-43 gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase... 80 3e-16 gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herb... 67 3e-12 >gi|300313771|ref|YP_003777863.1| Zn-dependent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 662 bits (1709), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/349 (94%), Positives = 343/349 (98%) Query: 1 MPVHAYGAHAADKPLEHLEITRRATGPHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVP 60 M VHAYGAHAADKPLEHL ITRRATG HDVQIDIAYCGICHSDLHQ+RSEWAGTQYPCVP Sbjct: 1 MQVHAYGAHAADKPLEHLHITRRATGAHDVQIDIAYCGICHSDLHQIRSEWAGTQYPCVP 60 Query: 61 GHEIVGRVAAVGNHVSKFKVGDLVGVGCIVDSCQTCPDCEEGLENYCDNMVGTYNGKTDD 120 GHEIVGRVAAVG HVSKFKVGD+VGVGCIVDSCQ+CPDC+EGLENYCDNMVGTYNGKTDD Sbjct: 61 GHEIVGRVAAVGAHVSKFKVGDVVGVGCIVDSCQSCPDCDEGLENYCDNMVGTYNGKTDD 120 Query: 121 APGWTMGGYSQQIVVHERYVLTVRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV 180 APGWTMGGYSQQIVVHERYVL +RHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV Sbjct: 121 APGWTMGGYSQQIVVHERYVLNIRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGV 180 Query: 181 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDF 240 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDF Sbjct: 181 VGIGGLGHMGIKIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFDF 240 Query: 241 ILNTVAAPHDLDAFLSLLKREGTMTLVGAPATPHPSPNVFSLIMKRRSLAGSLIGGIPET 300 ILNTVAAPHDLDAFL+LLKREGTMTLVGAPATPHPSPNVF+LI+KRRSLAGSLIGGIPET Sbjct: 241 ILNTVAAPHDLDAFLALLKREGTMTLVGAPATPHPSPNVFNLILKRRSLAGSLIGGIPET 300 Query: 301 QEMLDFCAKHNIVADIELIRAEEINTAYERMLKGDIKYRFVIDNASMAA 349 QEMLDFCA+H IVADIELIRA+EINTAYERMLKGD+KYRFVIDNAS+AA Sbjct: 301 QEMLDFCAEHGIVADIELIRADEINTAYERMLKGDVKYRFVIDNASLAA 349 >gi|300310297|ref|YP_003774389.1| alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 341 Score = 170 bits (430), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 112/335 (33%), Positives = 159/335 (47%), Gaps = 14/335 (4%) Query: 13 KPLEHLEITRRATGPHDVQIDIAYCGICHSDLHQVRSEW-AGTQYPCVPGHEIVGRVAAV 71 KPL ++ P + + G+CH+DLH +W P +PGHE G VAAV Sbjct: 15 KPLSIEQVPVPTPAPGQILVKFEASGVCHTDLHAAHGDWPVKPTPPFIPGHEGTGYVAAV 74 Query: 72 GNHVSKFKVGDLVGVGCIVDSCQTCPDCEEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQ 131 G V K GD VGV + +C C C G E C T G+++ G Sbjct: 75 GAGVKHVKEGDRVGVPWLHTACGCCSPCRTGWETLCAEQQNT---------GYSVNGSFA 125 Query: 132 QIVVHERYVLTVRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMGI 191 + + + + D AP+LCAG+T Y L+ PG+ V + GIGGLGHM + Sbjct: 126 EYGLADPKFVGHLPDNLDFGPAAPVLCAGVTVYKGLKETEVRPGEWVVISGIGGLGHMAV 185 Query: 192 KIAHAMGAHVVAFTTSDSKRADAKALGADEVVVSRNAEEMAAHAKSFD---FILNTVAAP 248 + A AMG HVVA + K A AK LGAD V RN +A + L T +P Sbjct: 186 QYAKAMGMHVVAADIHEDKLALAKKLGADLTVDGRNHNAVAEVQRIIGGAHGALVTAVSP 245 Query: 249 HDLDAFLSLLKREGTMTLVGAPATPHPSPNVFSLIMKRRSLAGSLIGGIPETQEMLDFCA 308 ++ L+ GTM LVG P P VF+ ++KR ++ GS++G + +E L F A Sbjct: 246 KAMEQAFGFLRARGTMALVGLPPGDISLP-VFNTVLKRITVRGSIVGTRQDLEESLVFAA 304 Query: 309 KHNIVADIELIRAEEINTAYERMLKGDIKYRFVID 343 + + A + + IN + RM +G I R V+D Sbjct: 305 EGKVAAHFTWDKLDNINAIFARMEEGKIDGRIVLD 339 >gi|300312106|ref|YP_003776198.1| zinc-type alcohol dehydrogenase transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 374 Score = 80.1 bits (196), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 89/308 (28%), Positives = 133/308 (43%), Gaps = 27/308 (8%) Query: 10 AADKPLEHLEITRRATGPHDVQIDIAYCGICHSDLHQVRSEWAGTQYPCVPGHEIVGRVA 69 A +PL+ E+ GP +V + + G+CHSDL V + P GHE V Sbjct: 18 AVSQPLKIEELDLDPPGPGEVLVRMKAAGLCHSDL-SVITGVRPRPVPMALGHEASAEVV 76 Query: 70 AVGNHVSKFKVGDLVGVGCIVDSCQTCPDCEEGLENYCD-----NMVGTYNGKTDDAPGW 124 VG+ V+ + GDLV V V SC C C EG C+ N VG Sbjct: 77 EVGSGVNDLRAGDLV-VLVFVPSCGHCLPCMEGRPALCEPGAATNTVGELLSGQRRLRDK 135 Query: 125 T--------MGGYSQQIVVHERYVLTVRHPEADLAAVAPLL--CAGITTY-SPLRHWGAG 173 T + ++ VV R + + EAD+ V L CA +T + + Sbjct: 136 TQPVHHHLGVSAFADHAVVSRRSCVKI---EADIDPVQAALFGCAVLTGVGAAVNTAEVK 192 Query: 174 PGKKVGVVGIGGLGHMGIKIAHAMGAH-VVAFTTSDSKRADAKALGADEVVVSRN---AE 229 G V+G+GG+G + A A GA V+A DSK A A++LGA V +R+ E Sbjct: 193 AGTTAAVLGLGGVGLCAMLGALASGAREVIAVDLHDSKLALARSLGATATVNARDPDAVE 252 Query: 230 EMAAHAK-SFDFILNTVAAPHDLDAFLSLLKREGTMTLVGAPAT-PHPSPNVFSLIMKRR 287 ++ A + D+ + ++A + +R G G PA H + SL+ + R Sbjct: 253 KVRALTRGGVDYAFEMAGSVQAMEAAYRMTRRGGMTVTAGLPAPGQHWALQQVSLVAEER 312 Query: 288 SLAGSLIG 295 ++ GS IG Sbjct: 313 TVKGSYIG 320 >gi|300310427|ref|YP_003774519.1| L-idonate 5-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 66.6 bits (161), Expect = 3e-12, Method: Compositional matrix adjust. Identities = 67/255 (26%), Positives = 108/255 (42%), Gaps = 18/255 (7%) Query: 24 ATGPHDVQIDIAYCGICHSDLHQVRSEWAGT---QYPCVPGHEIVGRVAAVGNHVSKFKV 80 A P +++ + GIC SDLH G + P V GHE+ G++A VG V+ Sbjct: 22 ALQPQQLKVKVKAGGICGSDLHYYHHGGFGVVRIKEPMVLGHEVSGQIAEVGTAVTDIAP 81 Query: 81 GDLVGVGCIVDSCQTCPDCEEGLENYCDNMVGTYNGKTDDAPGWTMGGYSQQIVVH--ER 138 G + + C C C+EG +N+C +M + G P G + Q+I++ + Sbjct: 82 GTRIAISP-SRPCGACKYCQEGKQNHCLDM--RFYGSAMRTP-HVQGAFRQEIIIERSQA 137 Query: 139 YVLTVRHPEADLAAVAPLLCAGITTYSPLRHWGAGPGKKVGVVGIGGLGHMGIKIAHAMG 198 +V+ + + A PL A +R G GK+V V G G +G + + A G Sbjct: 138 HVVADHVSDGEAAMAEPLSVA----LHAVRRAGPLVGKRVLVTGCGPIGALVVVAARRAG 193 Query: 199 AHVVAFTTSDSKRADAK-ALGADEVV-VSRNAEEMAAHAK---SFDFILNTVAAPHDLDA 253 A V T S A +GAD + ++ + A+A +FD + L Sbjct: 194 ALEVVVTDVASMPLQAALQVGADSAINMAETPAALKAYAADKGTFDVLFEASGNERALVG 253 Query: 254 FLSLLKREGTMTLVG 268 L L+ + + VG Sbjct: 254 ALEALRPQAIIVQVG 268 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.419 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,068,621 Number of extensions: 43113 Number of successful extensions: 155 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 150 Number of HSP's successfully gapped: 6 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)