BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0043.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 520 e-149 gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 223 2e-59 gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxido... 108 4e-25 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 107 8e-25 gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herb... 107 1e-24 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 520 bits (1340), Expect = e-149, Method: Compositional matrix adjust. Identities = 252/263 (95%), Positives = 255/263 (96%) Query: 1 MTAATPTVFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIATEPSEALV 60 MTAAT FEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIA EPS+ALV Sbjct: 1 MTAATTPAFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALV 60 Query: 61 ASLKDAAVAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKSEDVTPAYW 120 ASLKDA +APRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHK+ DVTPAYW Sbjct: 61 ASLKDAPIAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYW 120 Query: 121 DERLAVNLRHQFFCAQAVLPGMRERKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 DERLAVNLRHQFFCAQAVLPGMRERK GVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH Sbjct: 121 DERLAVNLRHQFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTH 180 Query: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALF 240 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLP RVDPHDVAALALF Sbjct: 181 GMARDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALF 240 Query: 241 LSSDSGAKCTGREYYVDAGWLGA 263 LSSDSGAKCTGREYYVDAGWLGA Sbjct: 241 LSSDSGAKCTGREYYVDAGWLGA 263 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 223 bits (567), Expect = 2e-59, Method: Compositional matrix adjust. Identities = 126/258 (48%), Positives = 165/258 (63%), Gaps = 1/258 (0%) Query: 5 TPTVFEHALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIATEPSEALVASLK 64 TP + A + SL GKRV ITGGG+GIGAA+VEAF QGA V F+DIATE SEAL + Sbjct: 4 TPQNVQLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVA 63 Query: 65 DAA-VAPRFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKSEDVTPAYWDER 123 A P F C+L ++ A +AT E++ +G D+L+NNAAND RHK E+VT YW++R Sbjct: 64 AAGHPKPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDR 123 Query: 124 LAVNLRHQFFCAQAVLPGMRERKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMA 183 +A+N R FF Q+V+ GM+ R GG I+NF SISWH +Y TAKA G+T G+A Sbjct: 124 IAINQRPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTRGLA 183 Query: 184 RDFGRDGVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALFLSS 243 RD G +RVN + PG + T RQ LW E + I QCL + P +A + LFL++ Sbjct: 184 RDLGPHKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAA 243 Query: 244 DSGAKCTGREYYVDAGWL 261 D A CT +E+ VDAGW+ Sbjct: 244 DDSAMCTAQEFIVDAGWV 261 >gi|300310426|ref|YP_003774518.1| gluconate 5-dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 264 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 74/244 (30%), Positives = 112/244 (45%) Query: 16 SLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIATEPSEALVASLKDAAVAPRFFPC 75 SL GK +ITG GIG AL GA + A+ A L+ Sbjct: 16 SLEGKLALITGSSGGIGLALARGMAQAGAAIVLNGRDAGKLAAVAAQLRAEGHTIHESSF 75 Query: 76 NLMNLEALRATFTEIETVMGGVDILINNAANDDRHKSEDVTPAYWDERLAVNLRHQFFCA 135 + + E ++A +IE+ +G + IL+NNA R+ E+ + W + ++ NL FF A Sbjct: 76 DATSSEEVKAAVQKIESEIGPIGILVNNAGIQRRNPLEEFKESDWHDLISTNLNSVFFAA 135 Query: 136 QAVLPGMRERKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRVNA 195 QAV M R G I+N S+ LG P + Y K ++ T GMA D+G+ G++VN Sbjct: 136 QAVARHMIPRGAGKIINICSVQSELGRPGIAPYTATKGAVKMFTKGMAIDWGKYGIQVNG 195 Query: 196 IIPGAIRTPRQTLLWHTPEEEAKILAAQCLPVRVDPHDVAALALFLSSDSGAKCTGREYY 255 + PG +T L + + ++ D D+ A+FL+SD+ G Y Sbjct: 196 LGPGYFKTEMNMALVNDAKFTDWLVGRTPSRRWGDVEDLVGAAVFLASDASRFVNGHILY 255 Query: 256 VDAG 259 VD G Sbjct: 256 VDGG 259 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 107 bits (268), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 87/256 (33%), Positives = 124/256 (48%), Gaps = 22/256 (8%) Query: 17 LAGKRVVITGGG--SGIGAALVEAFVGQGAQVCFLDIATEPSEALVASLKDAAVAPRFFP 74 L K VITGG +G+G A QGA+V LD+A A A + + Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGSQHLG---LV 59 Query: 75 CNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKSEDVTPAYWDERLAVNLRHQFFC 134 ++ + + I G +DIL+NNA K+ ++T A +D L V+LR + Sbjct: 60 ADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLYM 119 Query: 135 AQAVLPGMRERKGGVILNFGSISWH-----LGLPDLTLYMTAKAGIEGMTHGMARDFGRD 189 +QAVLP MR ++ G I+ S+S LG P Y AKAG+ G+ MAR+FG + Sbjct: 120 SQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPE 176 Query: 190 GVRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPV----RVDPHDVAALALFLSSDS 245 G+RVN+I PG I T ++ EE K A+ +P+ R D D+ +FL SD Sbjct: 177 GIRVNSITPGLIGTD---IIKGKLTEEKKAEIAETIPLARLGRAD--DIGGACVFLGSDL 231 Query: 246 GAKCTGREYYVDAGWL 261 CTG V+ G L Sbjct: 232 SQYCTGITLDVNGGML 247 >gi|300312738|ref|YP_003776830.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 75/252 (29%), Positives = 124/252 (49%), Gaps = 13/252 (5%) Query: 16 SLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIATEPSEALVASLKDAAVAPRFFPC 75 +L ++V++T G GIG A+ AFV GA V D++ + + A A V+ + Sbjct: 4 NLKDQKVIVTAGAQGIGLAITAAFVEAGAHVHICDVSEDFLASARARFGHAPVS--YSRT 61 Query: 76 NLMNLEALRATFTEI-ETVMGGVDILINNAA-NDDRHKSEDVTPAYWDERLAVNLRHQFF 133 ++ + + A F ++ + G +D+LINNA E+V + WD+ LAVNL F Sbjct: 62 DVSSEREVDAMFADLAQRWSGRLDVLINNAGIAGPTSPVEEVALSDWDQTLAVNLTGPFL 121 Query: 134 CAQAVLPGMRERKGGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDGVRV 193 C + +P +++ GG I+N S++ LG T Y +K G+ G+T A + G +RV Sbjct: 122 CTRRAVPLLKKNGGGAIVNISSVAGRLGFALRTPYSASKYGVIGLTETWAIELGPSHIRV 181 Query: 194 NAIIPGAIRTPRQTLLWHTP---------EEEAKILAAQCLPVRVDPHDVAALALFLSSD 244 NA++PG + RQ + E ++L+ L V D+A +FL S Sbjct: 182 NAVLPGIVEGARQERIVAAKAAAYGIGHEEMRQRLLSRVSLRKMVTAEDIANQVIFLCSP 241 Query: 245 SGAKCTGREYYV 256 +GA +G+ V Sbjct: 242 AGASISGQSLSV 253 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 890,997 Number of extensions: 38613 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 46 Number of HSP's gapped: 200 Number of HSP's successfully gapped: 46 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)