BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0042.p (320 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 573 e-165 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 238 4e-64 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 234 6e-63 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 104 1e-23 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 59 4e-10 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 573 bits (1476), Expect = e-165, Method: Compositional matrix adjust. Identities = 278/282 (98%), Positives = 282/282 (100%) Query: 1 MNKPHATPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGISQSGSDIS 60 MNKP+ATPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGI+QSGSDIS Sbjct: 1 MNKPNATPRRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDIS 60 Query: 61 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP 120 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP Sbjct: 61 PCNRIHLELAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYP 120 Query: 121 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS 180 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS Sbjct: 121 IDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLS 180 Query: 181 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAIAEALGMSLTGCSAIPAPYRERGQMA 240 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNA+AEALGMSLTGCSAIPAPYRERGQMA Sbjct: 181 AGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMA 240 Query: 241 YETGRRIVGMAYEDLRPSAILTREAFLDAIVVNAAIGGSTNA 282 YETGRRIVGMAYEDLRPSAILTR+AFLDAIVVNAAIGGSTNA Sbjct: 241 YETGRRIVGMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNA 282 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 238 bits (608), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 138/297 (46%), Positives = 185/297 (62%), Gaps = 4/297 (1%) Query: 9 RRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGISQSGSDISPCNRIHLE 68 R RS WF D +Y N GI E + G+P+IGI + S+++PCN + Sbjct: 10 RTLRSAGWFGTADKNGF--MYRSWMKNQGIPDHEFQ-GKPVIGICNTWSELTPCNAHFRK 66 Query: 69 LAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTT 128 +A+ VR GI +AGG P+EFP+ E+ RPTA + RNLA + + E + G PIDAVVL T Sbjct: 67 IAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLT 126 Query: 129 GCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEK 188 GCDKTTP+ +M AA+ D+PAIV++GGPML+G G +GSG+ +W+ + + AG I Sbjct: 127 GCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEITIHD 186 Query: 189 FLEIAAASAPSSGHCNTMGTASTMNAIAEALGMSLTGCSAIPAPYRERGQMAYETGRRIV 248 F+ A + S+G CNTMGTASTM +AE+LG+SL +AIPA R +A+ +G RIV Sbjct: 187 FMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIV 246 Query: 249 GMAYEDLRPSAILTREAFLDAIVVNAAIGGSTNAXGGQGAPCGRGAAFGRLDEVWLR 305 M +E L S ILTR+AF +AI NAAIGGSTNA A GR L++ W R Sbjct: 247 DMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELED-WTR 302 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 234 bits (598), Expect = 6e-63, Method: Compositional matrix adjust. Identities = 130/271 (47%), Positives = 171/271 (63%), Gaps = 3/271 (1%) Query: 12 RSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGISQSGSDISPCNRIHLELAK 71 RSQ WF D +Y N GI ++ GRP+IGI + S+++PCN LA+ Sbjct: 11 RSQAWFGRQDRDGF--IYRSWVKNRGIPHDQF-DGRPVIGICNTYSELTPCNSHFRALAE 67 Query: 72 RVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTTGCD 131 +V+ GI +AGG P+EFP+ + E RPTA + RNLA + + E + P+D VVL GCD Sbjct: 68 QVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLDGVVLLMGCD 127 Query: 132 KTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEKFLE 191 KTTP+ +M AA+VD+P I +SGGPML G G +GSG+ +W+ + + AG + E+F E Sbjct: 128 KTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAGKMTQEEFFE 187 Query: 192 IAAASAPSSGHCNTMGTASTMNAIAEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA 251 + S GHC TMGTASTM ++ EALG+ L +AIPA R +A GRRIV M Sbjct: 188 AESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARNAGRRIVQMV 247 Query: 252 YEDLRPSAILTREAFLDAIVVNAAIGGSTNA 282 EDL S ILTR+AF +AI VNAAIGGSTNA Sbjct: 248 EEDLVLSKILTRQAFENAIRVNAAIGGSTNA 278 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 77/253 (30%), Positives = 132/253 (52%), Gaps = 20/253 (7%) Query: 47 RPIIGISQSGSDISPCNRIHL-ELAKRVRDGIRDAGGIPMEFPLHPIFE-----NCRRPT 100 +PII + S + P +HL +L + V I AGG+ EF + + + R Sbjct: 34 KPIIAVVNSFTQFVP-GHVHLKDLGQLVAREIEAAGGVAKEFNTIAVDDSGTHGDARMLY 92 Query: 101 AAIDRNLAYLGLVEILHGYPIDAVVLTTGCDKTTPSQIMAAATVDIPAIVLSGGPMLDGW 160 + R+L + +++ + DA+V + CDK TP +MAA ++IP + +SGGPM G Sbjct: 93 SLPSRDLIADSVEYMVNAHCADAMVCISNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGK 152 Query: 161 MDGELVGSGSAIWK---GRKLLSAG--SIDNEKFLEIAAASAPSSGHCNTMGTASTMNAI 215 + + V + + K ++ AG +I + E+ ++ P+ G C+ M TA++MN + Sbjct: 153 VI-KTVNTDKKVIKLDLVDAMIQAGDKNISDADVAEVERSACPTCGSCSGMFTANSMNCL 211 Query: 216 AEALGMSLTGCSAIPAPYRERGQMAYETGRRIVGMA---YED----LRPSAILTREAFLD 268 E LG+SL G I A + +R ++ GR IV +A YE + P I T+E + + Sbjct: 212 TEVLGLSLPGNGTIVATHADRKELFLRAGRLIVELAKRHYEQDDYSILPRNICTKETWEN 271 Query: 269 AIVVNAAIGGSTN 281 A+ ++ ++GGSTN Sbjct: 272 AMTLDVSMGGSTN 284 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 59.3 bits (142), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 47/165 (28%), Positives = 77/165 (46%), Gaps = 11/165 (6%) Query: 121 IDAVVLTTGCDKTTPSQIMAAATV-DIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLL 179 DA + CDK P ++ A +PA+ + GPM G + + V K R+L Sbjct: 147 FDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSGMSNSDKV-------KIRQLY 199 Query: 180 SAGSIDNEKFLEIAAASAPSSGHCNTMGTASTMNAIAEALGMSLTGCSAIP--APYRE-R 236 +AG I + LE + S +G C GTA++ + EA+G+ L G + I P R+ Sbjct: 200 TAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAMGLHLPGAAFITPNTPLRDAL 259 Query: 237 GQMAYETGRRIVGMAYEDLRPSAILTREAFLDAIVVNAAIGGSTN 281 A + +I + + ++ ++ ++AIV A GGSTN Sbjct: 260 TAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALHATGGSTN 304 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,009,872 Number of extensions: 42365 Number of successful extensions: 145 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 141 Number of HSP's successfully gapped: 5 Length of query: 320 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 235 Effective length of database: 1,217,395 Effective search space: 286087825 Effective search space used: 286087825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)