BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0041.p (280 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 573 e-165 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 180 1e-46 gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 155 3e-39 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 79 3e-16 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 64 1e-11 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 573 bits (1476), Expect = e-165, Method: Compositional matrix adjust. Identities = 275/280 (98%), Positives = 279/280 (99%) Query: 1 MQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREMIYA 60 MQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREMIY Sbjct: 315 MQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREMIYP 374 Query: 61 FSAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDY 120 F+APLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDY Sbjct: 375 FAAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDDY 434 Query: 121 HARINDPALQIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGISTLPTLGDGRQSG 180 HARINDPAL+IDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRG+STLPTLGDGRQSG Sbjct: 435 HARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLPTLGDGRQSG 494 Query: 181 TSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEELARRKSEGIPPVPP 240 TSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNML+SEEELARRKSEGIPPVPP Sbjct: 495 TSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRKSEGIPPVPP 554 Query: 241 SQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 280 SQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH Sbjct: 555 SQTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 180 bits (457), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 103/283 (36%), Positives = 165/283 (58%), Gaps = 18/283 (6%) Query: 1 MQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQGRASNDREMIYA 60 +QP+GK+L E F+ AGG+PA++ +L+ + +TA GKT+ EN Q + + E+I + Sbjct: 311 LQPSGKFLMEDFYYAGGLPAVIRQLESV--IDKTALTANGKTLWENCQDAPNWNEEVIRS 368 Query: 61 FSAPLRERAGFLVLKGNLF-DFAIMKTSVISETFRERYLSTPGQENIFECRAVVFDGSDD 119 F P +E AG +LKGNL D A++K S + + RAVVF+ SDD Sbjct: 369 FDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKH-----------RGRAVVFENSDD 417 Query: 120 YHARINDPALQIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGISTLPTLGDGRQS 179 H RI+D L +DE +L ++ GP G+PG AE NM P ++++GI+ + + D R S Sbjct: 418 LHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDARMS 477 Query: 180 GTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEELARRKSEGIPP-V 238 GT+ +L+ SPE+A GG LA +++GD + +D+ + ++ +S+EELARR+++ P + Sbjct: 478 GTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQKPEL 537 Query: 239 PPS-QTPWQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 280 PP Q W ++Y V Q GA ++ + G +P+ NH Sbjct: 538 PPQMQRGWVKLYVDHVQQANQGADLDFLVGKSG--PYVPKDNH 578 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 155 bits (393), Expect = 3e-39, Method: Compositional matrix adjust. Identities = 98/288 (34%), Positives = 156/288 (54%), Gaps = 23/288 (7%) Query: 1 MQPAGKYLGERFHRAGGVPAIMWELQQAGKL-RAERITATGKTMAENLQGRASNDREMIY 59 +QP+G+YL E F+ AGG+PA++ L +A L + +T G+TM +N++ + E++ Sbjct: 313 LQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIYNDEVVR 372 Query: 60 AFSAPLRERAGFLVLKGNLFDF-AIMKTSVIS-ETFRERYLSTPGQENIFECRAVVFDGS 117 + PL E G +L+GNL A++K S + E + R RAVVF+ Sbjct: 373 PLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHR------------GRAVVFEDF 420 Query: 118 DDYHARINDPALQIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGISTLPTLGDGR 177 + Y RINDP L +D + +L ++ GP G+PG AEV NM P ++ G+ + + D R Sbjct: 421 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR 480 Query: 178 QSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLISEEELARRKSEGIPP 237 SGT+ IL+ +PE+A GG L ++DGD + +D G+ + ISEEE+ RR E Sbjct: 481 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRL-EARAK 539 Query: 238 VPPSQTP-----WQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 280 V + P +Q +Y V Q + G + + +G A +P+H+H Sbjct: 540 VLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAA--VPKHSH 585 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 79.3 bits (194), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 75/266 (28%), Positives = 119/266 (44%), Gaps = 54/266 (20%) Query: 6 KYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTMAENLQG---RASNDREMIYAFS 62 KY E HRAGG+ +I+ EL +AG L R T KT+ E ++ R S+D + F Sbjct: 324 KYHIEDVHRAGGIISILGELARAGLLDTSRPTVHSKTLGEAIEKFDIRVSSDPAVHKLFR 383 Query: 63 AP---------------------------LRER-------AGFLVLKGNLFDF-AIMKTS 87 A +R+R G VL GN+ + I+KT+ Sbjct: 384 AAPGGVPTQVAFSQEERFEANDLDRANGCIRDREHAYSQDGGLAVLYGNIAEKGCIVKTA 443 Query: 88 VISETFRERYLSTPGQENIFECRAVVFDGSDDYHARINDPALQIDENTLLAIRGAGPVGW 147 + E+ L G +FE + +G + D ++ ++ +R GP G Sbjct: 444 GVDESI----LKFSGTARVFESQDAAVEGI------LGD---KVKAGDVVIVRYEGPKGG 490 Query: 148 PGSAEVVNMQPPDALIKRGISTLPTL-GDGRQSGTSDSPSILNASPESAVGGGLAYLRDG 206 PG E+ + P + +G+ L DGR SG S I +ASPE+A GG + + +G Sbjct: 491 PGMQEM--LYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHASPEAAEGGAIGLVEEG 548 Query: 207 DRVRIDLNTGECNMLISEEELARRKS 232 D + ID+ N+ IS ++LA+R++ Sbjct: 549 DMIDIDIPNRTINLRISSDDLAKRRT 574 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 63.9 bits (154), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 72/284 (25%), Positives = 113/284 (39%), Gaps = 40/284 (14%) Query: 1 MQPAGKYLGERFHRAGGVPAIMWELQQAGKLRAERITATGKTM----------AENLQGR 50 + P G FH AGG ++ EL AG + + T G+ + AE GR Sbjct: 338 IYPNGTADVNHFHAAGGTGFVLRELIDAGLMHDDVTTILGQGLRQHCKEPMLAAEGENGR 397 Query: 51 -----------ASNDREMIYAFSAPLRERAGFLVLKGNLFDFAIMKTSVISETFRERYLS 99 S D ++ + P G +L+GNL A++K S + R Sbjct: 398 PGIVTWHDAPAQSGDEGVLGTVAKPFAADGGLKLLQGNL-GRAVIKISAVKPEHR----- 451 Query: 100 TPGQENIFECRAVVFDGSDDYHARINDPALQIDENTLLAIRGAGPVGWPGSAEVVNMQPP 159 + E AVVF + + A A Q+D + + I GP G E+ + P Sbjct: 452 ------VVEAPAVVFHSQEAFMAAFK--AGQLDRDFVAVITFQGPRA-NGMPELHALTPA 502 Query: 160 DALIKRGISTLPTLGDGRQSGTSDS-PSILNASPESAVGGGLAYLRDGDRVRIDLNTGEC 218 +++ + + DGR SG S P+ ++ +PE GG L +RDGD +R+D G Sbjct: 503 LGILQDKGRHVALVTDGRMSGASGKVPAAIHVTPEVLGGGPLGRVRDGDIIRLDAEAGTL 562 Query: 219 NMLISEEELARRKSEGIPPVPPSQTPWQE---IYRSTVGQLETG 259 + E A R + +E ++R+ VG E G Sbjct: 563 EAKVDATEWAARSQDSSDLSGNQHGMGRELFSLFRNAVGTAEEG 606 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 920,764 Number of extensions: 39028 Number of successful extensions: 122 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 117 Number of HSP's successfully gapped: 5 Length of query: 280 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 196 Effective length of database: 1,222,130 Effective search space: 239537480 Effective search space used: 239537480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)