BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0012.0040.p
         (308 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313781|ref|YP_003777873.1| metal-dependent hydrolase [Herb...   503   e-144
gi|300310385|ref|YP_003774477.1| metal-dependent hydrolase [Herb...   385   e-108

>gi|300313781|ref|YP_003777873.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 299

 Score =  503 bits (1296), Expect = e-144,   Method: Compositional matrix adjust.
 Identities = 241/298 (80%), Positives = 264/298 (88%)

Query: 10  MPPAPVRRIKGPMRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHAS 69
           MP  P  R K P+RIDAHQHFWRYRSEDYPWIG GMDLLAQDRLPYQFQPLL+AQGLHAS
Sbjct: 1   MPTPPAGRCKTPLRIDAHQHFWRYRSEDYPWIGAGMDLLAQDRLPYQFQPLLEAQGLHAS 60

Query: 70  ITVQARPGHEETGFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQD 129
           ITVQAR G +ET FLLEMARRD RIAG+I W+D+++P LAE+ME+WGR K+LGLRH +Q+
Sbjct: 61  ITVQARQGRDETAFLLEMARRDKRIAGIIGWEDLSAPDLAEQMERWGRHKLLGLRHQLQE 120

Query: 130 EDDAAQALVQPQFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPA 189
            DDA   L     + GVRWLQER Y L+VL HERHLPQLRQ CA HDQHWVVLNHLGKPA
Sbjct: 121 HDDANTLLTPAPLDQGVRWLQERSYTLDVLAHERHLPQLRQFCARHDQHWVVLNHLGKPA 180

Query: 190 LKEFRKGRAAYERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTAL 249
           LKEFRKGRAA+ERWRQ+LREL AMPHVACKISGLLTEADWLRGLCTRDF+HIVQCLDTAL
Sbjct: 181 LKEFRKGRAAFERWRQELRELTAMPHVACKISGLLTEADWLRGLCTRDFEHIVQCLDTAL 240

Query: 250 ELFGPQRLMFGSDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGLM 307
           +LFGPQRLMFGSDWPVCLLAASY+RVV VVREW+A RL P+DQDALWGESSARIYGL+
Sbjct: 241 DLFGPQRLMFGSDWPVCLLAASYSRVVTVVREWSATRLCPSDQDALWGESSARIYGLL 298


>gi|300310385|ref|YP_003774477.1| metal-dependent hydrolase
           [Herbaspirillum seropedicae SmR1]
          Length = 289

 Score =  385 bits (989), Expect = e-108,   Method: Compositional matrix adjust.
 Identities = 184/286 (64%), Positives = 223/286 (77%)

Query: 21  PMRIDAHQHFWRYRSEDYPWIGTGMDLLAQDRLPYQFQPLLDAQGLHASITVQARPGHEE 80
           P RIDAHQHFW Y+ + YPWIG GM+LLA DR P Q QPLLDA+GL  SI VQAR G EE
Sbjct: 3   PPRIDAHQHFWFYQPQAYPWIGAGMELLACDRTPGQLQPLLDAEGLGQSIAVQARAGREE 62

Query: 81  TGFLLEMARRDARIAGVIAWDDIASPQLAERMEQWGRPKMLGLRHPMQDEDDAAQALVQP 140
           TGFLLE+AR + RIAGV+ W+D+ASP LA  +E+WGR K++G RH +QDE D    +  P
Sbjct: 63  TGFLLELARGNRRIAGVVGWEDLASPHLAANVERWGRDKLVGFRHQLQDEADVGGCVDAP 122

Query: 141 QFNHGVRWLQERDYVLEVLTHERHLPQLRQLCALHDQHWVVLNHLGKPALKEFRKGRAAY 200
            FN GV WLQ +  V +VL + R + +++  CA HD+HW+VL+HLGKPAL EF +G  A+
Sbjct: 123 AFNAGVAWLQRQGMVYDVLVYARQMAEVQAFCARHDRHWLVLDHLGKPALTEFGRGDTAF 182

Query: 201 ERWRQQLRELAAMPHVACKISGLLTEADWLRGLCTRDFDHIVQCLDTALELFGPQRLMFG 260
           E W++QLR LAA+PHVACK+SGL+TEADW RGL  +DFD+I  CLDTALE FGPQRLMFG
Sbjct: 183 EGWQRQLRALAALPHVACKLSGLVTEADWQRGLRQKDFDNISLCLDTALEAFGPQRLMFG 242

Query: 261 SDWPVCLLAASYARVVAVVREWAARRLSPADQDALWGESSARIYGL 306
           SDWPVCLLAASYA+V  VVREWAA RLS A+Q+ LWG ++AR YGL
Sbjct: 243 SDWPVCLLAASYAKVAGVVREWAATRLSAAEQELLWGATAARCYGL 288


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.325    0.138    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,045,920
Number of extensions: 44634
Number of successful extensions: 151
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 151
Number of HSP's successfully gapped: 2
Length of query: 308
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 223
Effective length of database: 1,217,395
Effective search space: 271479085
Effective search space used: 271479085
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 130 (54.7 bits)