BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0035.p (447 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein... 376 e-105 gi|300312345|ref|YP_003776437.1| cell wall surface anchor protei... 255 4e-69 gi|300310420|ref|YP_003774512.1| inner membrane protein [Herbasp... 232 6e-62 gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088... 209 4e-55 >gi|300313785|ref|YP_003777877.1| autotransporter adhesin protein [Herbaspirillum seropedicae SmR1] Length = 3631 Score = 376 bits (965), Expect = e-105, Method: Compositional matrix adjust. Identities = 208/441 (47%), Positives = 286/441 (64%), Gaps = 8/441 (1%) Query: 10 SLKSYQMFDKLGSGATSFSLLQQGSSLDTLGAWLRLGLKPATAGGRDNVTLEMLGGFKRN 69 +LKSY +F++ + +++QQ S D LG+WL L D+V L LG F N Sbjct: 3196 NLKSYTLFNQPLNPGQEVAVMQQAGS-DALGSWLSFTLVRQAGRQADDVYLNHLGSFVLN 3254 Query: 70 GVDQHKVQPDLETGLSDVGLANIGLLYQLSVANTSSRPVIRVRFDSSNNVAGNVIELYEG 129 +D ++ + + S LAN+GLLYQ+ + P ++V +D+S G+V+ L+EG Sbjct: 3255 HLDLYRSETSRDKSQSGYDLANVGLLYQIDTHRPNKTPTLQVGYDASRAAVGDVVALFEG 3314 Query: 130 GRLVGSKTLVASDLGGGPKEVDVAVFQSLSAGRHDLEVRYTDTGGNSVN-SNLSVNVAAG 188 G ++ K L A+DLG G K V++ V QSL+ G HD+ RYTD GN+VN + V+VA G Sbjct: 3315 GTMLARKVLTAADLGSGTKTVNLTVAQSLAPGEHDIVARYTDLAGNTVNGTAQHVSVAGG 3374 Query: 189 GAPVTLSDLGVRGSKQSASEAQSLGQSGASYAVISDADTSFASGHGNQGPVFVGTVGGGS 248 G PVTL+DL VR SKQ+ + Q+L S YAVI+D DTS SGH GPV G VGGG Sbjct: 3375 GDPVTLTDLAVRSSKQAPAAVQALNASENRYAVINDLDTSDISGHSASGPVISGKVGGGR 3434 Query: 249 ASDRYLVTIQMGGKVVAFEDVGAGSFSIGIPAGVLPPGYYQDLSISATLLTPGARLGQTT 308 SDRY+VT++MGGKV+AF++VGAG FSI + AG L PG Y+DL+++A+ + G GQ+T Sbjct: 3435 DSDRYVVTVEMGGKVLAFDEVGAGDFSISLSAGSLAPGLYRDLTVTASRAS-GNEPGQST 3493 Query: 309 SMQGLKLGWYWAAQAGTSIVGGNGNDELVVGAAADPEATGGILVQTGAGDDRITVGSFGK 368 ++QGLKLGWYWAAQ+ + I GGNGND++V+G + VQTGAG D++ VG+FG+ Sbjct: 3494 AVQGLKLGWYWAAQSVSDIFGGNGNDDIVIGTT---HYSAASFVQTGAGQDKVIVGAFGR 3550 Query: 369 TSALRATVTDFTVGMDRVKVFGQIVNQDFVKNFVTASD-LN-GSTQLQIDLDGAGAGRLY 426 + L ATV DF +G+D+V+VF Q + Q + FVTAS +N T+L IDLDGAG G L Sbjct: 3551 SDNLAATVGDFVLGVDKVEVFNQSLTQANLSRFVTASAGVNPNDTRLSIDLDGAGPGTLT 3610 Query: 427 YHLTLQGVQYNPSTIGSIFGV 447 Y L LQ V YN + +IFG+ Sbjct: 3611 YTLMLQNVAYNSANTATIFGL 3631 >gi|300312345|ref|YP_003776437.1| cell wall surface anchor protein [Herbaspirillum seropedicae SmR1] Length = 7175 Score = 255 bits (652), Expect = 4e-69, Method: Compositional matrix adjust. Identities = 173/407 (42%), Positives = 230/407 (56%), Gaps = 18/407 (4%) Query: 51 TAGGRDNVTLEMLGGFKRNGVDQHKVQPD---LETGLSDVGLANIGLLYQLSVANTSSRP 107 TA + L GF D PD ++ G D+G N+ LL+Q AN +++P Sbjct: 6777 TAPDAKTLVLNAQAGFDDVNTDVFAFNPDGVPMDKGY-DIG--NLRLLFQAPTANVNTQP 6833 Query: 108 VIRVRFDSSNNVAGNVIELYEGGRLVGSKTLVASDLGGGPKEVDVAVFQSLSAGRHDLEV 167 I+V FD + AG+VI L EGG+++ S+TL +DLG + + SL AG+H L Sbjct: 6834 TIQVSFDGTRAAAGDVIRLMEGGQVLASRTLSDADLGSSNVTLSLTPASSLGAGQHTLSS 6893 Query: 168 RYTDTGGNSVNSN-LSVNVAAGGAPVTLSDLGVRGSKQSASEAQSLGQSGASYAVISDA- 225 +Y D GN+V N + NV G LS+L V G Q+A Q++ S YA++S A Sbjct: 6894 KYIDVAGNTVTGNDIVFNVQGGATAPVLSNLKVSGDGQAA---QAINDSTTRYAMVSAAA 6950 Query: 226 -DTSFASGHGNQGPVFVGTVGGGSASDRYLVTIQMGGKVVAFEDVGAGSFSIGIPAGVLP 284 D++ +SG +Q F GTVG + YLVT+ MGGK++AF AG F + PA VL Sbjct: 6951 GDSTGSSGLQHQLS-FTGTVGTAGSGHSYLVTVSMGGKLLAFNQFAAGDFKLEAPANVLA 7009 Query: 285 PGYYQDLSISATLLTPGARLGQTTSMQGLKLGWYWAAQAGTSIVGGNGNDELVVGAAADP 344 PG+Y+DLSI AT +T G GQ+T++ G LGWYWA Q+ T++ G G+D + +GAAA Sbjct: 7010 PGFYKDLSIVATDVTDGPSNGQSTAIGGQTLGWYWAPQSLTNLTAGAGDDMIPLGAAATA 7069 Query: 345 EATGGILVQTGAGDDRITVGSFGKTSALR--ATVTDFTVGMDRVKVFGQIVNQDFVKNFV 402 VQTGAG D + VG+ G T + R A V+DF VGMD+V V GQ V + FV Sbjct: 7070 NGIYNTEVQTGAGKDTLVVGASGATDSARLMARVSDFMVGMDKVSVAGQTVTAANLDRFV 7129 Query: 403 TAS--DLNGSTQLQIDLDGAGAGRLYYHLTLQGVQYNPSTIGSIFGV 447 TAS NG T L IDLDG G G L Y L L VQY S +IFGV Sbjct: 7130 TASAAPANG-TILSIDLDGPGPGSLTYTLLLVNVQYAQSNTHTIFGV 7175 >gi|300310420|ref|YP_003774512.1| inner membrane protein [Herbaspirillum seropedicae SmR1] Length = 1882 Score = 232 bits (591), Expect = 6e-62, Method: Compositional matrix adjust. Identities = 159/452 (35%), Positives = 244/452 (53%), Gaps = 25/452 (5%) Query: 6 TSIGSLKSYQMFDKLGSGATSFSLLQQGSSLDTLGAWLRLGLKP--ATAGGRDNVTLEML 63 T+ KSY +FDK + SF + Q GA+++ + ATA G+ + L Sbjct: 1446 TTFAEYKSYLVFDKPLTSGQSFGMAHQS------GAYVKGNMFSFTATADGK-GLVLTNK 1498 Query: 64 GGFKRNGVDQHKVQPDLETGLSDVGLANIGLLYQLSVANTSSRPVIRVRFDSSNNVAGNV 123 GG + +D ++ ++ + + NI +LYQ+ ++ P ++V+F++S G++ Sbjct: 1499 GGSYASNLDMYQFGGSEQSTNGNYDIGNIRILYQVDTGMSNLNPTVKVKFNASEASVGDI 1558 Query: 124 IELYEGGRLVGSKTLVASDLGGGPKEVDVAVFQSLSAGRHDLEVRYTDTGGNSVNSNLSV 183 + + G V SKTL A+DL V + + +S+ + L Y + GN ++N Sbjct: 1559 VSVRSGNS-VASKTLTANDLASPEASVSLTLAESILGSDNYLYTEYKEASGNIASANEIT 1617 Query: 184 NVAAGG----APVTLSDLGVRGSKQSASEAQSLGQSGASYAVISDADTSFASGHGNQGPV 239 A APV LSDL V + Q+L S YA ISD +A G+ +G + Sbjct: 1618 AYADRPQSVVAPV-LSDLNVASPNKGG--VQALNDSTTQYASISDPGAPWAPGNYERGLL 1674 Query: 240 FVGTVGGGSASDRYLVTIQMGGKVVAFEDVGAGSFSIGIPAGVLPPGYYQDLSISATLLT 299 F G VG +SD+YL+T++MG KV+AF+ V AG F++ A ++ PG ++DLS +AT +T Sbjct: 1675 FGGKVGAEGSSDKYLITVKMGSKVLAFDTVNAGDFTLSSAANLIAPGLHEDLSFTATNIT 1734 Query: 300 PGARLGQTTSMQGLKLGWYWAAQAGTSIVGGNGNDELVVGAAADPEATGGILVQTGAGDD 359 G G T+++ GLKLG YWA Q GG+GND ++GA + T L+QT GDD Sbjct: 1735 SGNNNGMTSTVTGLKLGHYWATQFLGDTRGGHGNDNFLLGATKNGLNT---LIQTNGGDD 1791 Query: 360 RITVGSFGKTSALRATVTDFTVGMDRVKVFGQIVN----QDFVKNFVTASDLNGSTQLQI 415 +T+G+FGK AT+TDFT G D++ VFG+ ++ Q FVK NG T++ + Sbjct: 1792 TLTLGAFGKQGNFAATITDFTAGADKIAVFGKSIDLGNLQQFVKEVAPIQGGNG-TKVVV 1850 Query: 416 DLDGAGAGRLYYHLTLQGVQYNPSTIGSIFGV 447 DLDGA AG Y L LQ V Y P+ ++FG+ Sbjct: 1851 DLDGAAAGNQTYTLHLQNVAYQPANTHTLFGI 1882 >gi|300310421|ref|YP_003774513.1| hypothetical protein Hsero_1088 [Herbaspirillum seropedicae SmR1] Length = 8016 Score = 209 bits (532), Expect = 4e-55, Method: Compositional matrix adjust. Identities = 137/374 (36%), Positives = 215/374 (57%), Gaps = 19/374 (5%) Query: 89 LANIGLLYQLSVANTSSRPVIRVRFDSSNNVAGNVIELYEGGRLVGSKTLVASDLGG-GP 147 + N+ LLYQ + AN +S P V +D + G+VI LYEG +L+ SKTL ++D+GG G Sbjct: 7647 MGNLRLLYQTTTANVNSTPTFAVSYDRARVAVGDVIGLYEGNKLLASKTLESADVGGTGT 7706 Query: 148 KEVDVAVFQSLSAGRHDLEVRYTD-TGGNSVNSNLSVNVAAGGAPVTLSDLGVRGSKQSA 206 +++ + SL+ G H + +YT TG S ++ +VN+ L+DL V+ Sbjct: 7707 ATLNLKISDSLAPGNHAIVSKYTSATGLTSQSTATTVNITGDAKTPLLTDLKVKAGYGEE 7766 Query: 207 SEAQSLGQSGASYAVISDADTSFASGHGNQGPVFVGTVG--GGSASDRYLVTIQMGGKVV 264 S A+++ G +Y I+D G ++G +F G+V G + +YLVT+ +GGK++ Sbjct: 7767 STAKAILLGGDAYTSITDPGRVGTEGTYDKGLIFSGSVNTPGLTGPQKYLVTVSLGGKLL 7826 Query: 265 AFE--------DVGAGSFSIGIPAGVLPPGYYQDLSISATLLTPGA-RLGQTTSMQGLKL 315 ++ D F++ + +L PG Y+DL+ + T +T G+ GQT+ ++ L Sbjct: 7827 GYDTFTLSTTDDPATSKFTLQAASNLLAPGLYRDLTATVTNVTEGSIHNGQTSVIKDASL 7886 Query: 316 GWYWAAQAGTSIVGGNGNDELVVGAAADPEATGGILVQTGAGDDRITVGSFGKTSALRAT 375 GWYW Q +++GG GND +++GA+ + T V TGAG +T+G+FGK+S L AT Sbjct: 7887 GWYWVGQGMGNLIGGQGNDGMLLGAS---QTTSPFTVTTGAGAGTLTLGAFGKSSNLTAT 7943 Query: 376 VTDFTVGMDRVKVFGQIVNQDFVKNF--VTASDLNGSTQLQIDLDGAGAGRLYYHLTLQG 433 VTDF +G+D+V+V+GQ + + +F TASD ST L +DLDG G L Y L LQ Sbjct: 7944 VTDFQLGLDKVQVWGQSITSANLSSFASATASDNGRSTTLTVDLDGVRGG-LSYTLQLQN 8002 Query: 434 VQYNPSTIGSIFGV 447 V +N + +IFGV Sbjct: 8003 VAFNAANTQTIFGV 8016 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.133 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,508,877 Number of extensions: 70707 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 198 Number of HSP's successfully gapped: 4 Length of query: 447 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 359 Effective length of database: 1,203,190 Effective search space: 431945210 Effective search space used: 431945210 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)