BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0033.p (242 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herb... 387 e-109 gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [H... 67 2e-12 gi|300309498|ref|YP_003773590.1| sugar transferase [Herbaspirill... 65 5e-12 gi|300309784|ref|YP_003773876.1| phosphatase/phosphohexomutase [... 60 2e-10 >gi|300313787|ref|YP_003777879.1| HAD-superfamily hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 387 bits (994), Expect = e-109, Method: Compositional matrix adjust. Identities = 188/215 (87%), Positives = 198/215 (92%) Query: 27 PITHFLFDLDGTLMDSDALHHAAFNTILSRWGRSVDVDYYKTHIMGASNAMIFDHLFPGM 86 PITHFLFDLDGTLMDSDALHHAAFNTIL+RW R +DV+YYKTHIMGASNAMIF HLFPGM Sbjct: 61 PITHFLFDLDGTLMDSDALHHAAFNTILARWDRHIDVEYYKTHIMGASNAMIFGHLFPGM 120 Query: 87 PASEYQPLAEEKEQLFRSQLDQQVAPTPGIERLLDHIARIGGRTAVVTNAPRANAELMLK 146 PA +Y PLAEEKE LFRSQLDQ+V PTPGIERLL+HI RIGGR+AVVTNAPRANAELMLK Sbjct: 121 PAEQYLPLAEEKESLFRSQLDQRVEPTPGIERLLEHIRRIGGRSAVVTNAPRANAELMLK 180 Query: 147 ATGLAGRFDTLVIGDELERAKPDPLPYLTALQLLGGTPDQAVAFEDSSSGVKAASSAQVW 206 ATGLAG+FDTLVIGDEL RAKPDPLPYLT LQLLGG AVAFEDSSSGVKAASSA +W Sbjct: 181 ATGLAGQFDTLVIGDELARAKPDPLPYLTGLQLLGGEAGAAVAFEDSSSGVKAASSAGIW 240 Query: 207 TFGMLGGLDEARLRAAGAHAVIRDFNGQALWDKIG 241 TFGMLGGLDEARLRAAGAHAVIRDFN ALWDK+G Sbjct: 241 TFGMLGGLDEARLRAAGAHAVIRDFNAAALWDKLG 275 >gi|300313180|ref|YP_003777272.1| phosphoglycolate phosphatase [Herbaspirillum seropedicae SmR1] Length = 226 Score = 66.6 bits (161), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 56/184 (30%), Positives = 81/184 (44%), Gaps = 7/184 (3%) Query: 26 TPITHFLFDLDGTLMDSDALHHAAFNTILSRWGRSVDVDYYKTHIMGASNAMIFDHL--- 82 T I + DLDGT++D+ A H A N + G + + +G + + Sbjct: 5 TNIKAAIIDLDGTMLDTAADFHVAVNRMRDDLGLTPLSQETIVNFVGKGTENLIRRVLAV 64 Query: 83 -FPGMPASEY--QPLAEEKEQLFRSQLDQQVAPTPGIERLLDHIARIGGRTAVVTNAPRA 139 + A++Y Q LA E D + PG+ L + G R A VTN P A Sbjct: 65 DYAEDEAAQYFQQALAACTEHYLAINGDYS-SLYPGVLEGLQALRNKGLRLACVTNKPLA 123 Query: 140 NAELMLKATGLAGRFDTLVIGDELERAKPDPLPYLTALQLLGGTPDQAVAFEDSSSGVKA 199 A +L+ GL F+ + GD + KPDP+P L Q P Q VA DSS+ +A Sbjct: 124 FAVPLLQKKGLRDFFEIVYGGDSFPKKKPDPMPLLQVCQDFALAPAQVVAIGDSSNDAQA 183 Query: 200 ASSA 203 A +A Sbjct: 184 ARAA 187 >gi|300309498|ref|YP_003773590.1| sugar transferase [Herbaspirillum seropedicae SmR1] Length = 215 Score = 65.1 bits (157), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 57/209 (27%), Positives = 93/209 (44%), Gaps = 21/209 (10%) Query: 22 NTHSTPITHFLFDLDGTLMDSDALHHAAFNTILSRWGRSVDVDYYKTHIMGASNAMIFDH 81 N +T +FD DGTL+DS+ + A++ ++ +G + + G S + H Sbjct: 2 NDFATSPALVIFDCDGTLVDSEVVAARAWSEYVATYGVQLTPEDALARFRGVSMSWCIAH 61 Query: 82 LFPGMPASEYQPLAEEKEQLFRSQ----LDQQVAPTPGIERLLDHIARIGGRTAVVTNAP 137 + Q L EQ R++ L+Q + P G L+ + ++ A+ +NAP Sbjct: 62 V----AQLRGQALPAHFEQELRARMGVMLEQHLQPINGA---LEMVEQLQIPFALASNAP 114 Query: 138 RANAELMLKATGLAGRFDTLVIGD-ELERAKPDPLPYLTALQLLGGTPDQAVAFEDSSSG 196 EL L+ TGL F + +++R KPDP +L A + LG P + EDS G Sbjct: 115 HHKIELCLRVTGLLPHFHGRIFSAYDVQRWKPDPALFLFAAERLGVPPARCAVVEDSLPG 174 Query: 197 VKAASSAQVWTFGMLGGLDEARLRAAGAH 225 V+A G+ G+ L+ G H Sbjct: 175 VQA---------GLAAGMKVIALQEHGVH 194 >gi|300309784|ref|YP_003773876.1| phosphatase/phosphohexomutase [Herbaspirillum seropedicae SmR1] Length = 244 Score = 59.7 bits (143), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 62/228 (27%), Positives = 93/228 (40%), Gaps = 26/228 (11%) Query: 28 ITHFLFDLDGTLMDSDALH----HAAFNTILSRW--------GRSVDVDYYKTHIMGASN 75 ITH + D DG L+DS+++ H +L W G + H A Sbjct: 7 ITHLICDCDGVLLDSESIALRVLHRELLPLLPAWSGAGGADDGEQEEQRSAALHAAIAHR 66 Query: 76 AMIFDHLFPGMPASEYQ-----PLAEEKEQLFRSQLDQQVAPTPGIERLLDHIARIGGRT 130 ++ + G E++ AE Q + P PG+ L I R+ Sbjct: 67 LGMYTDILLGEVDMEFELGLDAAQAERIHQTVALACGDEALPVPGVVEALQAI-RLPRAV 125 Query: 131 AVVTNAPRANAELMLKATGLAGRFDTLVI-GDELERAKPDPLPYLTALQLLGGTPDQAVA 189 A ++APR A L + GL +F + G ++ KP P YL A G P + VA Sbjct: 126 ASNSDAPRIEAGL--RRCGLYPQFAGRIYSGHDVAHPKPAPDVYLAAAAGFGVDPSRCVA 183 Query: 190 FEDSSSGVKAASSAQVWTFGMLGGLDE-----ARLRAAGAHAVIRDFN 232 +DS +GV+AA +A + G G + +L+ AGAH V D Sbjct: 184 VDDSVTGVRAAVAAGMHVLGFTGVAHDRKTMARKLKDAGAHQVFEDMK 231 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 830,848 Number of extensions: 35381 Number of successful extensions: 127 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 125 Number of HSP's successfully gapped: 4 Length of query: 242 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 159 Effective length of database: 1,226,865 Effective search space: 195071535 Effective search space used: 195071535 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)