BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0019.p (132 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313805|ref|YP_003777897.1| two component response regulato... 164 3e-42 gi|300310669|ref|YP_003774761.1| two component response regulato... 68 2e-13 gi|300313791|ref|YP_003777883.1| response regulator protein [Her... 65 1e-12 gi|300311805|ref|YP_003775897.1| two component hybrid sensor his... 54 3e-09 >gi|300313805|ref|YP_003777897.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 125 Score = 164 bits (414), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 91/124 (73%), Positives = 101/124 (81%) Query: 9 PPSHRPIAVIDDDLGVRAALCNLLDSAGHRHCSFDGGEDFLTSTCLPVAACAIVDLNLPG 68 P SH IAVIDDD GVRAALCNLLDSAG+R C F GE+FL S C+PVA CAI+DL L Sbjct: 2 PSSHGAIAVIDDDHGVRAALCNLLDSAGYRSCRFSSGEEFLASDCMPVAVCAIIDLQLAC 61 Query: 69 MSGFELAEQLRSLRPDLPVLFISAQVTPLQRQRATAMGMALLVKPVDADTLLVLLTSTLL 128 MSGFELAE+L RPDLP+LFISAQVTP Q+QRA+A+G ALL KPVDADTLL LLT+ LL Sbjct: 62 MSGFELAERLALARPDLPLLFISAQVTPRQQQRASALGGALLAKPVDADTLLALLTACLL 121 Query: 129 YGPP 132 YG P Sbjct: 122 YGRP 125 >gi|300310669|ref|YP_003774761.1| two component response regulator protein [Herbaspirillum seropedicae SmR1] Length = 214 Score = 68.2 bits (165), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 42/125 (33%), Positives = 67/125 (53%), Gaps = 4/125 (3%) Query: 6 ETTPPSHRPIAVIDDDLGVRAALCNLLDSAGHRHCSFDGGEDFLTSTCLPVAACAIVDLN 65 ET PP + ++DDD VR +L LL S R F+G E FL + +C I+D+ Sbjct: 3 ETNPPV---VYIVDDDEAVRTSLAWLLSSVNIRTECFEGPEAFLKAYDANSLSCLILDVR 59 Query: 66 LPGMSGFELAEQLRSLRPDLPVLFISAQVTPLQRQRATAMG-MALLVKPVDADTLLVLLT 124 +P +SGF+L ++L+ + D+PV+F+S RA G + L KP ++ +L + Sbjct: 60 MPEISGFQLQDRLKEIGADIPVIFVSGHGDIPMSVRALHNGAVDFLEKPYNSQQMLERIQ 119 Query: 125 STLLY 129 TL + Sbjct: 120 MTLKH 124 >gi|300313791|ref|YP_003777883.1| response regulator protein [Herbaspirillum seropedicae SmR1] Length = 206 Score = 65.5 bits (158), Expect = 1e-12, Method: Compositional matrix adjust. Identities = 40/110 (36%), Positives = 56/110 (50%), Gaps = 1/110 (0%) Query: 12 HRPIAVIDDDLGVRAALCNLLDSAGHRHCSFDGGEDFLTSTCLPVAACAIVDLNLPGMSG 71 R + V+DDD VR AL LL SAG+ F ++FL + V C ++D+ +PG G Sbjct: 2 ERTVFVVDDDNMVRTALLRLLASAGYAAHGFASADEFLQARLPAVPCCLLLDMKMPGSDG 61 Query: 72 FELAEQLRSLRPDLPVLFISAQVT-PLQRQRATAMGMALLVKPVDADTLL 120 FE+ +L +PV+FIS T P Q A L KP+ + LL Sbjct: 62 FEVVRRLERRGDAIPVIFISGYGTIPTTVQAMKAGAHEFLTKPIQDERLL 111 >gi|300311805|ref|YP_003775897.1| two component hybrid sensor histidine kinase/response regulator protein [Herbaspirillum seropedicae SmR1] Length = 1278 Score = 54.3 bits (129), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 36/107 (33%), Positives = 52/107 (48%), Gaps = 2/107 (1%) Query: 17 VIDDDLGVRAALCNLLDSAGHRHCSFDGGEDFLTSTCLPVAACAIVDLNLPGMSGFELAE 76 V++D++ + +C LL++ GH+ GE+ L + D+NLPGMSG ELA+ Sbjct: 1168 VVEDNIDSQQLVCELLETLGHQARGVISGEEALQALEEQGFDVLFTDVNLPGMSGVELAK 1227 Query: 77 QLRSLRPDLPVLFISAQVTPLQRQRATAMGMALLVKPVDADTLLVLL 123 PDL V+F S T + R L KP D D L +L Sbjct: 1228 AALGRFPDLAVIFASGYGTMVARN--VGFAAHALPKPYDIDQLQEIL 1272 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.138 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 414,669 Number of extensions: 16210 Number of successful extensions: 125 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 134 Number of HSP's successfully gapped: 6 Length of query: 132 Length of database: 1,619,870 Length adjustment: 75 Effective length of query: 57 Effective length of database: 1,264,745 Effective search space: 72090465 Effective search space used: 72090465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 125 (52.8 bits)