BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0010.p (323 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 553 e-159 gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 86 5e-18 gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 62 7e-11 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 553 bits (1425), Expect = e-159, Method: Compositional matrix adjust. Identities = 276/323 (85%), Positives = 289/323 (89%) Query: 1 MQASASGARAGQQEVRRVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVAR 60 MQ S + + RVAVVGTGVIGASWAAYFLA+GLEVSA DPMP AE KLRAA+AR Sbjct: 1 MQQQVSIKGKTKSGIARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIAR 60 Query: 61 HWPSLELLGLATGASPERLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAH 120 HWPSL+ LGLA AS ERLRF LE ALEGADFVQENGPEREDLKIDLFRRMDAALPA Sbjct: 61 HWPSLQALGLAEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQ 120 Query: 121 VVLASSSSGLLMSKVQAACRHPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFY 180 VVLASSSSGLLMSKVQAACRHP RVVLGHPFNPPHLIPLVEVIGGAQT+PEAVQ ALDFY Sbjct: 121 VVLASSSSGLLMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFY 180 Query: 181 TAIGKKAIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG 240 AIGKK IHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG Sbjct: 181 AAIGKKPIHVRKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMG 240 Query: 241 PYLNLHMSGGEGGIGHMLDHLGPPIESWWADLGAPSITAALKAQIRAGVEEQMSGKTSAG 300 PYLNLHMSGGEGGI H+LDHLG PIESWWADLGAP++T ALKA +RAGVEEQM+GKTS Sbjct: 241 PYLNLHMSGGEGGIQHLLDHLGAPIESWWADLGAPALTDALKATVRAGVEEQMAGKTSTD 300 Query: 301 IAAERDQLLVQLLAAKSKTQVIP 323 IAAERDQLLVQLLAAKS+ +VIP Sbjct: 301 IAAERDQLLVQLLAAKSRARVIP 323 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 85.5 bits (210), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 84/285 (29%), Positives = 129/285 (45%), Gaps = 18/285 (6%) Query: 18 VAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSL----ELLGLATG 73 VAV+G+G +GA A G V D A K A+ + L +L Sbjct: 16 VAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNLDKLVSKDKLTPALAQ 75 Query: 74 ASPERLRFSASLEEALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSGLLMS 133 A+ +R+ + SLEE L AD V E E+ K +LF +++A + +LA+++S L ++ Sbjct: 76 AASQRISVAQSLEE-LHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSSLSIT 134 Query: 134 KVQAACRHPGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKAIHVRKE 193 ++ A +HP RVV H FNP ++ LVE+I G + Q D A GK + R Sbjct: 135 QIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDTALAWGKTPVMARS- 193 Query: 194 VKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPYLNLHMSGGEGG 253 G I NRL + E ++ +G A ID + G R MGP+ + + Sbjct: 194 TPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFR---MGPFELMDL------ 244 Query: 254 IGHMLDHLGPPIESWWADLGAPSITAALKAQ--IRAGVEEQMSGK 296 IGH ++ + A P T +L Q + AG + SG+ Sbjct: 245 IGHDVN-FAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGR 288 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 61.6 bits (148), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 57/195 (29%), Positives = 90/195 (46%), Gaps = 8/195 (4%) Query: 15 VRRVAVVGTGVIGASWAAYFLARGLEVSATDPMPDAEVKLRAAVARHWPSLELLGLATGA 74 V++VAV+G GV+GA AA+ + + V D +P E V R +L+ L A Sbjct: 16 VKKVAVLGAGVMGAQIAAHCINARVPVVLFD-LPAKEGPKNGIVQRAIENLKKLSPAPLG 74 Query: 75 SPER--LRFSASLEE---ALEGADFVQENGPEREDLKIDLFRRMDAALPAHVVLASSSSG 129 + + L A+ E+ LEG D + E ER D K DL++++ + + AS++SG Sbjct: 75 NKDDAALIQVANYEDDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNTSG 134 Query: 130 LLMSKVQAACRH--PGRVVLGHPFNPPHLIPLVEVIGGAQTAPEAVQAALDFYTAIGKKA 187 L ++ + R H FNPP + LVE+I T P + F T K Sbjct: 135 LSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLGKG 194 Query: 188 IHVRKEVKGHIANRL 202 + + +ANR+ Sbjct: 195 VVRAFDTPNFVANRV 209 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,085,226 Number of extensions: 49401 Number of successful extensions: 230 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 227 Number of HSP's successfully gapped: 3 Length of query: 323 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 238 Effective length of database: 1,217,395 Effective search space: 289740010 Effective search space used: 289740010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)