BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0008.p (209 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313812|ref|YP_003777904.1| hypothetical protein Hsero_4530... 167 7e-43 >gi|300313812|ref|YP_003777904.1| hypothetical protein Hsero_4530 [Herbaspirillum seropedicae SmR1] Length = 195 Score = 167 bits (422), Expect = 7e-43, Method: Compositional matrix adjust. Identities = 103/199 (51%), Positives = 132/199 (66%), Gaps = 7/199 (3%) Query: 1 MDRSVVFTPREPVLPGGIFLNCLCLLAISGYLGLLLYLQSMALLPGTPLRQLQYTGIILT 60 M+RS+VFTPREPV+PGG+ LN LCLLA+S L LLY + +P L LQ TGIILT Sbjct: 1 MNRSIVFTPREPVMPGGLVLNGLCLLAVSNCLVALLYTEWYLRVPPCELCMLQRTGIILT 60 Query: 61 GIGFLRNLRFGIKSAHYGVALVGALLTGLMALRQISLEALAGQTPTGPLLQGMHFHTWMA 120 G+GF++NL G+KSAHYGVAL+GALLTGL+ALRQ+ ++ +A L + A Sbjct: 61 GMGFMKNLCQGVKSAHYGVALLGALLTGLIALRQLWMQPVAAMLSQPVCL-------FAA 113 Query: 121 LFSLAAMLTIAALLGVKSAEYPATLLLLQSAEKENEPRRIPWGRIGAGTIFVLLAGAHLV 180 LF+L A++ IA LLGVKSAEYP TLLLLQ+AE +E +PWGRI +F+ L Sbjct: 114 LFALVAIIVIACLLGVKSAEYPGTLLLLQTAEATDEKHGVPWGRILVCAVFIATVAVTLS 173 Query: 181 ASMGGCGRLVCGEGLIDLL 199 SM C CG +++ L Sbjct: 174 GSMINCTSATCGASVLERL 192 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.143 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 575,407 Number of extensions: 19666 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 1 Length of query: 209 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 128 Effective length of database: 1,236,335 Effective search space: 158250880 Effective search space used: 158250880 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 128 (53.9 bits)