BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0012.0006.p (362 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC trans... 702 0.0 gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC trans... 256 2e-69 gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC trans... 236 3e-63 gi|300312694|ref|YP_003776786.1| sugar ABC transporter ATPase [H... 224 1e-59 gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [H... 223 1e-59 >gi|300313814|ref|YP_003777906.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 362 Score = 702 bits (1812), Expect = 0.0, Method: Compositional matrix adjust. Identities = 343/362 (94%), Positives = 351/362 (96%) Query: 1 MTELSVNDLHLDYGTGASANPILKGVSMQLQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 MTELSVNDLHLDYGTGA ANPILKGVSM+LQRGEVVALLGPSGSGKTTLLRAVAGLESPK Sbjct: 1 MTELSVNDLHLDYGTGAHANPILKGVSMELQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 Query: 61 AGTIDIGTRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSAEI 120 AG IDIG RRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPS EI Sbjct: 61 AGAIDIGKRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSGEI 120 Query: 121 AKRVKEVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 A RVK VLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE Sbjct: 121 ATRVKAVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 Query: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQTPNTLFT 240 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQ PNTLFT Sbjct: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQAPNTLFT 240 Query: 241 AEFMGSNNKLAAEVVSREGDRVTVKLDGGTAVATLRGREDAKPGEQLTGIIRVEQVRIAG 300 AEFMGSNNKLAAEV+SREG+RVTVKLDGGTAVATLRGR D +PGEQLTGIIRVEQVRI+G Sbjct: 241 AEFMGSNNKLAAEVLSREGERVTVKLDGGTAVATLRGRSDVRPGEQLTGIIRVEQVRISG 300 Query: 301 EQTGNAIRLPLSTCMYLGDRWECLFKVEGGPAGSVGLRAYAPQRLDQGEYWLSLPEQALW 360 E + NAIRLPLSTCMYLGDRWECLFKV+GGPAGSVGLRAYAPQRLDQGEYWLSLPEQ+LW Sbjct: 301 EASDNAIRLPLSTCMYLGDRWECLFKVDGGPAGSVGLRAYAPQRLDQGEYWLSLPEQSLW 360 Query: 361 AF 362 AF Sbjct: 361 AF 362 >gi|300310414|ref|YP_003774506.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 376 Score = 256 bits (655), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 137/325 (42%), Positives = 195/325 (60%), Gaps = 9/325 (2%) Query: 20 NPILKGVSMQLQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGTIDIGTRRVFDGARNFE 79 N I+K + + ++RGE + LLGPSGSGKTT L +AG E P G I + DG Sbjct: 35 NLIVKNLDLDIRRGEFLTLLGPSGSGKTTCLMMLAGFEFPTGGEIRL------DGQLLNR 88 Query: 80 MPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSAEIAKRVKEVLSQLGLGHLGER 139 +P +RN+G+VFQ+YAL+PH T+A NVAY L +RK A +VK+ L + +G G+R Sbjct: 89 VPPHKRNIGMVFQNYALFPHMTIAQNVAYPLSVRKMDRATREAKVKQALDMVQMGKFGDR 148 Query: 140 YPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGLSALM 199 YP QLSGGQQQRVA+AR LV++PQ++L+DEPL LD +LRE + L+ L RLG++ + Sbjct: 149 YPTQLSGGQQQRVALARTLVFDPQLVLMDEPLGALDKQLREHMQLELKALHKRLGVTFVY 208 Query: 200 VTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQTPNTLFTAEFMGSNNKLAAEVVSREG 259 VTHDQ EA+ +SDR+ + + G I+Q +Y+ P+ F A F+G NN+ V S +G Sbjct: 209 VTHDQSEALTMSDRVAVFDQGVIQQLAPVTELYEYPDNQFVANFIGDNNRFKGSVESVQG 268 Query: 260 DRVTVKLDGGTAVATLRGREDAKPGEQLTGIIRVEQVRIA--GEQTGNAIRLPLSTCMYL 317 + TV+L G A+ L A G+ +T +R E++R+A G N+IR ++ +Y Sbjct: 269 EHCTVRLTDGAALTGLNVHR-AHAGQSITACVRPERIRLAAGGSAEPNSIRASIAGLIYF 327 Query: 318 GDRWECLFKVEGGPAGSVGLRAYAP 342 GD V G V L P Sbjct: 328 GDHVRVRCTVAGQEDCFVKLSLSDP 352 >gi|300309936|ref|YP_003774028.1| spermidine/putrescine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 361 Score = 236 bits (601), Expect = 3e-63, Method: Compositional matrix adjust. Identities = 139/317 (43%), Positives = 194/317 (61%), Gaps = 20/317 (6%) Query: 17 ASANPILKGVSMQLQRGEVVALLGPSGSGKTTLLRAVAGLESPKAGTIDIGTRRVFDGAR 76 A+ L+ + +Q+ GE + LLGPSG GKTT LR +AGLE P G RV G Sbjct: 20 ANGTRALQPLDLQIHPGETLVLLGPSGCGKTTTLRMIAGLEFPDEGG------RVLFGEE 73 Query: 77 NFEM-PAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSAEIAKRVKEVLSQLGLGH 135 + M P E+R +G+VFQ+YAL+P+ +V +N+AYG+K+RK + E A+RV+ +L + L Sbjct: 74 DVTMLPIEKRGVGMVFQNYALFPNMSVGENIAYGMKIRKIAAGERAERVERLLEMVHLQG 133 Query: 136 LGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLREEARAFLRELIVRLGL 195 L R QLSGGQ+QRVA+ARAL P+V+LLDEPL+ LDAKLRE R+ L +L+ LG+ Sbjct: 134 LSHRRVDQLSGGQKQRVALARALAMEPRVLLLDEPLTALDAKLREAVRSDLNKLLRSLGI 193 Query: 196 SALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQTPNTLFTAEFMGSNNKLAAEVV 255 +A+ VTHDQGEAMA+ DRI+++ G+I Q GTPQ +Y P F AEF+G+ N++ V Sbjct: 194 TAIYVTHDQGEAMALGDRIVVMERGKISQIGTPQEIYYHPANDFVAEFIGAMNRVDGIVA 253 Query: 256 SREGDRVTVKLDGGTAVATLRGREDAKPGEQLTGIIRVEQVRIAG--EQTGNAIRLPLST 313 GDR+ + TL DA G+ + R E V + E + +R L Sbjct: 254 ---GDRLQLP------TGTLPLPPDAVAGQPAQALFRPEDVEVIDITEADAHCLRGKLIN 304 Query: 314 CMYLGDRWECLFKVEGG 330 +LGDR F++E G Sbjct: 305 TFFLGDRTR--FEIEIG 319 >gi|300312694|ref|YP_003776786.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 224 bits (570), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 135/312 (43%), Positives = 186/312 (59%), Gaps = 28/312 (8%) Query: 1 MTELSVNDLHLDYGTGASANPILKGVSMQLQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 M ++ L Y G P+L + + + GE V LLGPSG GK+T+LR +AGLE Sbjct: 1 MADIHCQALAKHYAGGP---PVLHPLDLHIGDGEFVVLLGPSGCGKSTMLRMIAGLEDIS 57 Query: 61 AGTIDIGTRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSAEI 120 GT+ IG V D +PA ERN+ +VFQ+YAL+PH +V DN+A+GL+ K P+AEI Sbjct: 58 GGTLRIGGTVVND------LPARERNVAMVFQNYALYPHMSVYDNIAFGLRRLKRPAAEI 111 Query: 121 AKRVKEVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 +RV+EV + L L L ER P +SGGQQQR AIARA++ P V L DEPLSNLDAKLR Sbjct: 112 DRRVREVAALLNLEALLERKPRAMSGGQQQRAAIARAIIKTPSVFLFDEPLSNLDAKLRA 171 Query: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQTPNTLFT 240 + R ++ L RL + + VTHDQ EAM ++DR++L+ +GRI Q G+P +Y+ P LF Sbjct: 172 QLRGDIKRLHQRLRTTTVYVTHDQLEAMTLADRVILMQDGRIVQAGSPAELYRYPRNLFA 231 Query: 241 AEFMGSNN-KLAAEVVSREGDRVTVKLDGGTAVATLRGREDAKPGEQLTGI--------- 290 A F+G+ + V R+ DG + T R A GE+ + + Sbjct: 232 AGFIGTPAMNFLSGTVQRQ--------DGQLFIETAHQRW-ALTGERFSRLRHAMAVKLA 282 Query: 291 IRVEQVRIAGEQ 302 +R + VRIAGE+ Sbjct: 283 VRPDHVRIAGER 294 >gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 223 bits (569), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 137/348 (39%), Positives = 199/348 (57%), Gaps = 17/348 (4%) Query: 1 MTELSVNDLHLDYGTGASANPILKGVSMQLQRGEVVALLGPSGSGKTTLLRAVAGLESPK 60 M +S+ +L Y N +++ ++++++ GE V +GPSG GK+TLLR +AGLE Sbjct: 1 MAAVSIRNLAKRYDD----NEVMRDINLEIEDGEFVVFVGPSGCGKSTLLRMIAGLEEIS 56 Query: 61 AGTIDIGTRRVFDGARNFEMPAEERNLGLVFQSYALWPHKTVADNVAYGLKLRKTPSAEI 120 G +DIG RR+ E+PA +R + +VFQSYAL+PH ++ DN+A+GLK+ AEI Sbjct: 57 DGDLDIGARRMN------EVPASKRGVAMVFQSYALYPHMSLYDNMAFGLKIAGKSKAEI 110 Query: 121 AKRVKEVLSQLGLGHLGERYPHQLSGGQQQRVAIARALVYNPQVILLDEPLSNLDAKLRE 180 V+ L + HL +R P LSGGQ+QRVAI RA+ P V L DEPLSNLDA LR Sbjct: 111 DAAVQHAAKILHIDHLLDRKPRALSGGQRQRVAIGRAITRQPSVFLFDEPLSNLDAALRV 170 Query: 181 EARAFLRELIVRLGLSALMVTHDQGEAMAISDRILLLNNGRIEQQGTPQSMYQTPNTLFT 240 + R +L L + + VTHDQ EAM ++D+I++L+ GRIEQ G+PQ +Y P F Sbjct: 171 KMRLEFAKLHDDLKTTMIYVTHDQIEAMTLADKIVVLSEGRIEQVGSPQQLYHHPANRFV 230 Query: 241 AEFMGSN--NKLAAEVVSREGDRVTVKLDGGTAVATLRGREDAKPGEQLTGIIRVEQVRI 298 A F+GS N + V + + D V V+L GG + G+++T +R E + I Sbjct: 231 AGFIGSPKMNFIDGTVAAIQADGVQVQLPGGGLQWAAVDGSTLQVGQKVTLGVRPEHLNI 290 Query: 299 AGEQTGNAIRLPLSTCMYLGDRWECLFKVEGGPAGSVGLRAYAPQRLD 346 A Q A++ + LGD + L+ G ++ LR P LD Sbjct: 291 AQGQA--ALQARCTALELLGD-FSYLYAAYEGSEDALILR--VPDSLD 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.135 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,203,268 Number of extensions: 53108 Number of successful extensions: 632 Number of sequences better than 1.0e-08: 133 Number of HSP's gapped: 390 Number of HSP's successfully gapped: 150 Length of query: 362 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 276 Effective length of database: 1,212,660 Effective search space: 334694160 Effective search space used: 334694160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)