BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0042.p (636 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 1122 0.0 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 164 2e-41 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 155 9e-39 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 149 9e-37 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 148 1e-36 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 1122 bits (2903), Expect = 0.0, Method: Compositional matrix adjust. Identities = 567/634 (89%), Positives = 601/634 (94%) Query: 1 LTRSERFHEVFAAAKEARRPLLFGQIIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGA 60 LTRSERFHEVFAAAKEARRPL+FGQ+IIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGA Sbjct: 432 LTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGA 491 Query: 61 MILSVTFIPAAIALFIGEKVAEKENFIMVGAKRLYEPALKVIMENKAVVLTFAAALIVVC 120 MILSVTFIPAAIALFIGEKVAEKEN +M+ AKRLYEP LK IMENK VVLTFA ALI+VC Sbjct: 492 MILSVTFIPAAIALFIGEKVAEKENILMLWAKRLYEPMLKTIMENKPVVLTFAGALILVC 551 Query: 121 GVVATRLGAEFVPSLNEGDIAIQALRIPGTSLTQSIEMQKSIEKALVKKFPEVERVFARS 180 GVVA+RLG+EFVPSLNEGD+AIQALRIPGTSL+QS+EMQK IEK L +KFPEVERVFAR+ Sbjct: 552 GVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIEKTLKQKFPEVERVFART 611 Query: 181 GTAEVASDPMPPNISDGYIMLKPEAQWPEPKKTHAELLTAIQAEVAKIPGNNYEFSQPIQ 240 GTAEVASDPMPPNISDGYIMLKP+ QWPEPKK+ ELL AIQ EVAKIPGN+YEFSQPIQ Sbjct: 612 GTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSREELLEAIQDEVAKIPGNSYEFSQPIQ 671 Query: 241 LRFNELISGVRSDVAVKIFGDDMNVLNDTAGKISAVLEKIPGATEVKIEQTTGLPMLTVN 300 LRFNELISGVRSDVAVK+FGDDM VLNDTA KISAVLEKIPGATEVKIEQTTGLPMLTV Sbjct: 672 LRFNELISGVRSDVAVKVFGDDMKVLNDTASKISAVLEKIPGATEVKIEQTTGLPMLTVE 731 Query: 301 IDRQKTARYGLNVSDIQDTIATAIGGKEAGTLFQGDRRFGIVVRLPDNSRTDLEAIRRLP 360 IDR+KTARYGLNVSDIQDT+ATAIGGKEAGT+FQGDRRFGIVVRLPD +RTDLEAIRRLP Sbjct: 732 IDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLP 791 Query: 361 IALPLQDGNTRTSYVPLGEVAALDIAPGPNQISREDGKRRIVVSANVRGRDLGGFVADAE 420 IALP Q+G TR SYVPLGEVA+L IAPGPNQISREDGKRRIVVSANVRGRDLGGFVA+AE Sbjct: 792 IALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSANVRGRDLGGFVAEAE 851 Query: 421 RQLQAQVKIPTGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFVMFNNVKDGMLVF 480 RQLQ+ VK+P GYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLF MF+NVKDG+LVF Sbjct: 852 RQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVF 911 Query: 481 TGIPFALTGGILALALRDIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRSLDQA 540 TGIPFALTGGILALA+R IPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGR+LD A Sbjct: 912 TGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAA 971 Query: 541 IHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLP 600 IHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLP Sbjct: 972 IHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLP 1031 Query: 601 LLYKLAHNKDEDEAPAEAGTRRPFWKFWAKRSST 634 LLYKLAH+KDE++ PA +R PFWKFW K+SST Sbjct: 1032 LLYKLAHDKDEEDNPAGPPSRPPFWKFWVKQSST 1065 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 164 bits (415), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 173/635 (27%), Positives = 304/635 (47%), Gaps = 43/635 (6%) Query: 7 FHE-VFAAAKEARRPLLFGQIIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSV 65 H+ + A E P + I IV++PMF L+GV +F P+A VV A+ + +LS Sbjct: 420 LHDAIIVGAGEIGVPAFVATLCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASYLLSR 479 Query: 66 TFIPAAIALFIGE--KVAEKENFIMVGAKRLYEPALKVIMENKAVVLT--------FAAA 115 T +P + L +G +V + ++ R ++ + + +VL+ FA A Sbjct: 480 TLVPTLVMLLMGSHGQVDSAKQSLLQRLYRAFDNRFERVRHGYTLVLSSLLAHRRRFALA 539 Query: 116 LIVVC---GVVATRLGAEFVPSLNEGDIAIQALRIP-GTSLTQSIEMQKSIEKALVKKFP 171 + C ++ LG +F P+++ G I + +R+P GT + ++ +E+ + P Sbjct: 540 FLGFCLLSCLLYPFLGRDFFPNVDGGQIRLH-MRMPTGTRIEETARAADEVERVIRTLIP 598 Query: 172 --EVERVF-----ARSGTAEVASDPMPPNISDGYIMLKPEAQWPEPKKTHAELLTAIQAE 224 E+E + SG S+ DG I++ E + L++ ++AE Sbjct: 599 ASELETILDNLGVPNSGINLSYSNAGTIGTLDGEILMA----LKEGHRPTEALVSMLRAE 654 Query: 225 VAK-IPGNNYEFSQPIQLRFNELISGVRSDVAVKIFGDDMNVLNDTAGKISAVLEKIPGA 283 + + PG + F QP + L G+ + + V+ G +M A +++ + +IPGA Sbjct: 655 LPRRFPGIEF-FFQPADIVTQILNFGLPAAIDVQFTGPNMEANARLAAELTKQIRQIPGA 713 Query: 284 TEVKIEQTTGLPMLTVNIDRQKTARYGLNVSDIQDTIATAIGGK-EAGTLFQGDRRFGIV 342 + + Q P L + +DR + ++GL+ S++ + A+ G + F + G+V Sbjct: 714 VDAHVHQRLDGPSLDLRMDRTRLQQFGLSASNVGQNLLIALAGSSQTQPAFWLNPNNGVV 773 Query: 343 ----VRLPDNSRTDLEAIRRLPIALPLQDGNTRTSYVPLGEVAALDIAPGPNQI--SRED 396 V+ P L+A+ +P++ P + +T + LG + +D+ G SR + Sbjct: 774 YNIAVQSPQYQVDSLDALLNIPVS-PGANASTTPPQL-LGNL--VDVHAGRQMAVASRYN 829 Query: 397 GKRRIVVSANVRGRDLGGFVADAERQLQAQVK--IPTGYWTTWGGQFENLQSATQRLQIV 454 I V +V+G DL VA L Q+K + G GQ E +QS+ L + Sbjct: 830 IAPAIDVYVSVQGTDLAS-VAGKVSALVEQIKPKLARGSQVVIRGQVETMQSSFTGLGVG 888 Query: 455 IPVALLMVFVLLFVMFNNVKDGMLVFTGIPFALTGGILALALRDIPMSISAAVGFIALSG 514 + +A+++V++L+ V F + D ++ +P AL G L + +S+ A G I G Sbjct: 889 LAMAIVLVYLLVVVNFQSWIDAAIIIAALPAALAGIAWMLFITGTTLSVPALTGAIMTMG 948 Query: 515 VAVLNGLVMISFIRSLREEGRSLDQAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGA 574 VA N +++++F R REEG + A E TR+RPVLMTAL +G +PMAL G GA Sbjct: 949 VATANSILIVAFARQRREEGATPLAAALEAGSTRIRPVLMTALAMIIGMIPMALGLGEGA 1008 Query: 575 EVQRPLATVVIGGILSSTALTLLVLPLLYKLAHNK 609 E PL VIGG++ +T TL +P+++ H + Sbjct: 1009 EQNAPLGRAVIGGLMLATVSTLFFVPVVFAGVHQR 1043 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 155 bits (392), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 144/595 (24%), Positives = 266/595 (44%), Gaps = 38/595 (6%) Query: 26 IIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFIG--EK--VA 81 ++++ V++PM G G ++ + ++VA+++ +++++ T PA A + EK Sbjct: 447 LVLIAVFIPMAFFGGAVGAIYRQFSLSMVASMVFSVLMAFTLTPALCATILKPVEKGHHH 506 Query: 82 EKENFIMVGAKRLYEPALKVI-----MENKAVVLTFAAALIVVC-GVVATRLGAEFVPSL 135 EK+ F ++ A M NKA LIV C G++ RL A F+P+ Sbjct: 507 EKKGFFGWFNRKFNSTASGYQGIIGGMLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNE 566 Query: 136 NEGDIAIQALRIPGTSLTQSIEMQKSIEKALVKKFPEVERVFARSGTAEVASDPMPPNIS 195 ++G I PG S +++ + K IE+ +K P VE A G + N Sbjct: 567 DQGYIITNVQLPPGASTSRTTAVVKQIEEYYLKD-PAVEHAIAVLGYS------FSGNGQ 619 Query: 196 DGYIMLKPEAQWPEPKKTHAELLTAIQAEVAKIPGNNYEFSQPIQLRFNEL-ISGVRSDV 254 + ++ P W + K+ + A K G + I N I + + Sbjct: 620 NAALVFTPLKDWSQRDKSQSA-----DAIAGKAFGAFSQIKDAIVFPLNPPPIPELGNAT 674 Query: 255 AVKIFGDDMNVLNDTA-----GKISAVLEKIPGATEVKIEQTTGLPMLTVNIDRQKTARY 309 D L TA +I + + V+ E P L ++IDR K Sbjct: 675 GFTFRLQDRAGLGHTALVQARNQILGMASQSKVLAGVRPEGLEDAPQLQLDIDRDKANAL 734 Query: 310 GLNVSDIQDTIATAIGGKEAGTLFQGDRRFGIVVRLPDNSRTDLEAIRRLPIALPLQDGN 369 G+ S I ++TA+G R+ ++V+ R E + L N Sbjct: 735 GVPFSTINSVLSTALGSSYVNDFPNLGRQQRVIVQADAKRRLQPEDLTNL------YARN 788 Query: 370 TRTSYVPLGEVAALDIAPGPNQISREDGKRRIVVSANVR-GRDLGGFVADAERQLQAQVK 428 + + VP GP Q+ R +G + ++ GR G +A+ E + K Sbjct: 789 SSGTMVPFSAFVTAKWINGPVQLIRYNGYPAMKIAGGAAPGRSTGEAMAEMESFV---AK 845 Query: 429 IPTGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFVMFNNVKDGMLVFTGIPFALT 488 +P G+ W GQ +++ + + ++L+ VF++L ++ + + V +P + Sbjct: 846 LPPGFGFEWTGQSLEEKTSGNQAPALYALSLIAVFLVLAALYESTSIPLAVMLVVPLGIL 905 Query: 489 GGILALALRDIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRSLDQAIHEGAMTR 548 G +L + LR +P + VG IA+ G++ N +++I F + L+ +G+ L +A E R Sbjct: 906 GALLGVTLRAMPNDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLEAVHLR 965 Query: 549 LRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLY 603 RP++MT+L LG +P+A+A+G G+ QR + T V+GG++++T L + ++P+ + Sbjct: 966 FRPIIMTSLAFILGVLPLAIASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFF 1020 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 149 bits (375), Expect = 9e-37, Method: Compositional matrix adjust. Identities = 148/600 (24%), Positives = 267/600 (44%), Gaps = 43/600 (7%) Query: 26 IIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFI----GEKVA 81 +++ V++PM G G ++ + T+V+A+ ++++++T PA A + G V Sbjct: 449 MVLSAVFIPMAFFGGSTGVIYRQFSITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQ 508 Query: 82 EKENFIMVG-----AKRLYEPALKVIMENKAVVLTFAAALIVVCGVVATRLGAEFVPSLN 136 + F + Y+ + +++ L A + V V+ RL F+P + Sbjct: 509 QGRFFTWFNRSFDRSATRYQGGVAGLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEED 568 Query: 137 EGDIAIQALRIPGTSLTQSIEMQKSIEKALVKKFPEVERVFARSGTAEVASDPMPPNISD 196 +G + Q G + Q+ + K++++ + K VE V A G A V + N + Sbjct: 569 QGVLMAQIQLPTGATAEQTATVVKTVQEYFMNKPESVESVLAIVG-AGVGGNSQ--NGAR 625 Query: 197 GYIMLKPEAQWPEPKKTHAELLTAIQAEVAKIPGNNYEFSQPIQLRFNELISGVRSDVAV 256 +I LK Q ++ +EL+ A+++KI G P +R SG D+ + Sbjct: 626 AFIRLKDWEQRTGAGQSASELVRRANADLSKIRGARVFLMNPPAVRGLGQSSGF--DLEL 683 Query: 257 KIFG----DDMNVLNDT---AGKISAVLEKIPGATEVKIEQTTGL---PMLTVNIDRQKT 306 K G D + D + +A+L + +TTGL P L V ID +K Sbjct: 684 KDVGGVGHDALLQARDQFLQLARSNALLANV---------RTTGLDDTPQLRVAIDDRKA 734 Query: 307 ARYGLNVSDIQDTIATAIGGKEAGTLFQGDRRFGIVVRLPDNSRTDLEAIRRLPIALPLQ 366 + SDI T+ATA+GG R + V+ R + + R + Sbjct: 735 DALSIATSDINATLATAMGGTYVNDFLSNGRVKKVYVQGDSAFRMQADDLNRWYVR---- 790 Query: 367 DGNTRTSYVPLGEVAALDIAPGPNQISREDGKRRI-VVSANVRGRDLGGFVADAERQLQA 425 N + VP + GP+ + R +G +V G G +A+ E+ L A Sbjct: 791 --NAASQMVPFAAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAGVSSGTAMAEVEK-LVA 847 Query: 426 QVKIPTGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFVMFNNVKDGMLVFTGIPF 485 Q +P G W G + + + ++ +++L VF+ L ++ + V +P Sbjct: 848 Q--LPAGIGFEWTGSSYQERLSGNQAPMLYAISILFVFLCLAALYESWSVPFAVILAVPL 905 Query: 486 ALTGGILALALRDIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRSLDQAIHEGA 545 + G +L + + + VG + G++ N ++++ F + L E G+SL +A E Sbjct: 906 GIIGTVLLTGIFGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHESGKSLWEATLEAV 965 Query: 546 MTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLYKL 605 RLRP+LMT+L G +P+A+A+G G+ +R + T V+GG+LS+T L + +PL Y L Sbjct: 966 QLRLRPILMTSLAFMFGVLPLAVASGAGSASRRAIGTGVLGGMLSATLLGIFFVPLFYYL 1025 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 148 bits (374), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 96/319 (30%), Positives = 168/319 (52%), Gaps = 13/319 (4%) Query: 291 TTGLPM---LTVNIDRQKTARYGLNVSDIQDTIATAIGGKEAGTLFQGDRRFGIVVRLPD 347 + GLP + + IDR K A G++ S I DT++ A+G R ++++ Sbjct: 711 SDGLPAGASVQLEIDRHKAAALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQA 770 Query: 348 NSRTDLEAIRRLPIALPLQDGNTRTSYVPLGEVAALDIAPGPNQISREDGKRRIVVSANV 407 ++R LE + +L + N VPL E+ P Q+ R G + +S + Sbjct: 771 SARMQLEDVMKLYVR------NQSGGMVPLAELVKASWREAPTQLQRYQGFPAVRISGSA 824 Query: 408 R-GRDLGGFVADAERQLQAQVKIPTGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLL 466 G G +A+ ER Q ++P G+ W GQ + + + +++ +++L+VF++L Sbjct: 825 APGVASGEAMAEMERLAQ---QLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVL 881 Query: 467 FVMFNNVKDGMLVFTGIPFALTGGILALALRDIPMSISAAVGFIALSGVAVLNGLVMISF 526 ++ + + V +P L G + A+ LRD+P + VG I + G++ N ++++ F Sbjct: 882 AALYESWSIPLSVMLVVPLGLVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEF 941 Query: 527 IRSLREEGRSLDQAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIG 586 R LR EG L +A + ++ RLRP+LMT+L +LG VP+ LA+G AE QR + T V G Sbjct: 942 ARQLRSEGMGLVEAAIKASVLRLRPILMTSLAFALGVVPLMLASGASAETQRAIGTGVFG 1001 Query: 587 GILSSTALTLLVLPLLYKL 605 G++S+T L +L +P+ + L Sbjct: 1002 GMVSATVLAVLFVPVFFVL 1020 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,795,542 Number of extensions: 72835 Number of successful extensions: 315 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 294 Number of HSP's successfully gapped: 15 Length of query: 636 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 545 Effective length of database: 1,188,985 Effective search space: 647996825 Effective search space used: 647996825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)