BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0032.p (256 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein... 60 1e-10 gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrog... 60 3e-10 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 60 3e-10 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 57 1e-09 >gi|300313009|ref|YP_003777101.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 245 Score = 60.5 bits (145), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 67/245 (27%), Positives = 103/245 (42%), Gaps = 42/245 (17%) Query: 7 LLLADIRVEHAQAAAAVLSDAGFDAEVTSVDISSRE-SIQVLVGKASSLGNVSGLIHAAG 65 +L R + A AA ++ G V D+++RE + QVL+ G G++ AG Sbjct: 34 VLHCHARRDAADQVAAEIAALGRQVRVLQFDVANREQAAQVLLADVEQHGCYYGVVCNAG 93 Query: 66 VSPSQASP-------QTILKVDLYGTAVILEEFGNVIERGGSGVVIASQSGHRLGPLSVE 118 ++ A P ++L+ +L G F NV L PL + Sbjct: 94 ITRDNAFPAMPGEDWDSVLRTNLDG-------FYNV-----------------LNPLVMP 129 Query: 119 QSRSLATTPTEELLSLPFLAPEKVTDSLHAYQLSKRGNALRVMAEAVRWGKRGARVNTIS 178 R A L S+ L + + Y +K G A A+ KR VN ++ Sbjct: 130 MVRRRAAGRIVTLASVSGLIGNRGQVN---YSAAKAGVIGATKALALELAKRDITVNCVA 186 Query: 179 PGIIITPLANDELRGPRSEGYRRMLELSPVGRAGTPDEVASVAALLLGADGAFITGSDFL 238 PG+I T + D P E +L+L P RAG P+EVA+ A L+ D A+IT Sbjct: 187 PGLIDTDMTED---APIDE----ILKLIPARRAGKPEEVAAAVAFLMSPDAAYITRQVIS 239 Query: 239 MDGGV 243 ++GG+ Sbjct: 240 VNGGL 244 >gi|300313865|ref|YP_003777957.1| 3-alpha-hydroxysteroid dehydrogenase/carbonyl reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 59.7 bits (143), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 55/212 (25%), Positives = 97/212 (45%), Gaps = 9/212 (4%) Query: 37 DISSRESIQVLVGKASSLGNVSGLIHAAGVSPSQASPQTILKVDLYGTAVILEEFGNVIE 96 D+SS E+I +V + + L + AGV P A Q + +V+ G +++ + Sbjct: 51 DLSSAEAIDAMVAQLPE--RIDALCNIAGV-PGTAEAQLVARVNYLGLRHLVQRLTARLS 107 Query: 97 RGGSGVVIASQSGHRLGPLSVEQSRSLATTPTEELLSLPFLAPEKVTDSLHAYQLSKRGN 156 GG+ V +AS G P +EQ ++L+ + +LA V H YQ K Sbjct: 108 PGGAIVNVASILGAEW-PQRLEQHKALSQAASFAE-GAAWLAQHPVAQE-HCYQYFKEAL 164 Query: 157 ALRVMAEAV-RWGKRGARVNTISPGIIITPLANDELRGPRSEGYRRMLELSPVGRAGTPD 215 + M +A + + R+N ++PG + TP+ D + E ++ + + R D Sbjct: 165 IVWSMTQAQPMFLQHSVRMNCVAPGPVFTPILGDFVTMLGQERVQK--DAHRMKRPAYAD 222 Query: 216 EVASVAALLLGADGAFITGSDFLMDGGVTSSY 247 E+A+ L D +I G + +DGG+ S+Y Sbjct: 223 EIAAAIVWLCSDDSRWINGVNLPVDGGLASTY 254 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 59.7 bits (143), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 61/226 (26%), Positives = 101/226 (44%), Gaps = 32/226 (14%) Query: 22 AVLSDAGFDAEVTSVDISSRESIQVLVGKA-SSLGNVSGLIHAAGVSPSQASPQTILKVD 80 A + + GFD + ++S+ ES + K + +G V L++ AG++ + + K D Sbjct: 46 ATMREQGFDIHASEGNVSNWESTKAAFEKVRAEIGEVDVLVNNAGIT-RDGQFRKMSKDD 104 Query: 81 LYGTAVI---LEEFGNVIERGGSGVVIASQSGHRLGPLSVEQSRSLATTPTEELLSLPFL 137 AVI L NV ++ VI G ++++ + Sbjct: 105 W--DAVIDTNLNSLFNVTKQ-----VIEGMCERGFG----------------RIINISSV 141 Query: 138 APEKVTDSLHAYQLSKRGNALRVMAEAVRWGKRGARVNTISPGIIITPLANDELRGPRSE 197 +K Y +K G MA A +G VNT+SPG I T D +R R E Sbjct: 142 NGQKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGT----DMVRAIRPE 197 Query: 198 GYRRMLELSPVGRAGTPDEVASVAALLLGADGAFITGSDFLMDGGV 243 +++ PV R G P+E+AS+ A + ++G + TGSDF ++GG+ Sbjct: 198 VLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGGL 243 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 57.4 bits (137), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 64/240 (26%), Positives = 102/240 (42%), Gaps = 25/240 (10%) Query: 4 GKKLLLADIRVEHAQAAAAVLSDAGFDAEVTSVDISSRESIQVLVGK-ASSLGNVSGLIH 62 G +L+ADI ++ A A L AG A +D+ S ESI G L++ Sbjct: 32 GHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGSPESIAAAFAHIEKDYGRCDVLVN 91 Query: 63 AAGVSPSQASPQTILKVDLYGTAVILEEFGNVIERGGSGVVIASQSGHRLGPLSVEQSRS 122 AGV+ +T +D LE + + +GV++A Q RL V+Q Sbjct: 92 NAGVA------RTYSFLDY-----PLENWLQTMNVNVTGVLLAGQHAARL---MVKQQWG 137 Query: 123 LATTPTEELLSLPFLAPEKVTDSLHAYQLSKRGNALRVMAEAVRWGKRGARVNTISPGII 182 ++++ ++ + AY SK A+ + G VN+++PG + Sbjct: 138 -------RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPGPV 190 Query: 183 ITPLANDELRGPRSEGYRRMLELSPVGRAGTPDEVASVAALLLGADGAFITGSDFLMDGG 242 TP+ E Y R++ P+ R GT DE+AS L + A+ITG +DGG Sbjct: 191 DTPMTQAMHSEQTRESYYRLV---PMRRYGTTDEIASAVCYLAAEESAYITGHVIPVDGG 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.314 0.132 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 847,290 Number of extensions: 36519 Number of successful extensions: 174 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 194 Number of HSP's successfully gapped: 6 Length of query: 256 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 173 Effective length of database: 1,226,865 Effective search space: 212247645 Effective search space used: 212247645 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 129 (54.3 bits)