BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0029.p (457 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 82 7e-17 gi|300310281|ref|YP_003774373.1| TonB-dependent siderophore rece... 80 2e-16 gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein... 80 2e-16 gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore rece... 80 4e-16 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 76 6e-15 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 82.4 bits (202), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 100/375 (26%), Positives = 165/375 (44%), Gaps = 34/375 (9%) Query: 33 PLLCLAAPVQAEESANALPAITIN-AQSPLPLQNQVSVGSNLDLTPLQ-TPASVSVINRQ 90 PL L P A++ ALPAI ++ A++ +V PL+ P +++V+ Q Sbjct: 20 PLAIL--PACAQQDTTALPAIEVSGARANDGYVAPTAVSGTKSEAPLRDVPQTINVVPAQ 77 Query: 91 QLEERGDASITDAITRAPGYSAMGHPGNSGSALSARGFTDATSVMRLYDGLRQYGGVGVS 150 + ++ +S+ D + PG G RGFT + DG R Sbjct: 78 VIRDQNASSLQDVLKNVPGLGLSTGDGQRDQVF-IRGFTAIGD--QFVDGFRD----DAL 130 Query: 151 FPFDTWNIDRIEVLSGPASVIYGDGAIGGVVNVIPKKPTRGKIENEIETTIGTHNTRRLG 210 + D N++ +EV+ GPA+V+YG G+ GG++N + KKP G +++ T + RR Sbjct: 131 YFRDLSNVESVEVIKGPAAVLYGRGSSGGLINRVTKKP--GVDVSDVSFTANSTAGRRAE 188 Query: 211 LGSGGAINEMLSYRVDIAGDRSDG-----WVDRGNTNNLSLSGALRLDVNRDLFVQLSYA 265 L G A LS+R+ A +R D ++DR ++ S ALR + L +Q+ Y Sbjct: 189 LDLGRAGEGALSWRLTGAVERGDSFRAQQFIDR---TAIAPSVALRFSADTKLLLQMDYL 245 Query: 266 QGHQRPMAYFGTPLINGQQVDALRERNY---NVADGTIDYKDRWAQLEAQWTP--DADTT 320 + R M FG P G+ VD Y N D + R A A +T + + + Sbjct: 246 E--DRRMMDFGIPSYRGRPVDVDPGTTYGARNARDADF-VQSRVASTTATFTHRFNDEFS 302 Query: 321 LRT--RLYQINSNRYWRNAESYVYNKTTGLVDRGDATEIRHDQSQTGVTTDATFKGHLAG 378 R R Y + NR N N+ T A +R D++ ++ T K L Sbjct: 303 FRNGLRYYDYHLNR--NNTNGGTVNEATATTTLNHAKLVR-DENGWFNQSELTQKLQLGS 359 Query: 379 LKNQVSVGFDINSTS 393 +++++ G ++ S S Sbjct: 360 IRHEILYGLELGSQS 374 >gi|300310281|ref|YP_003774373.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 715 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 62/186 (33%), Positives = 95/186 (51%), Gaps = 6/186 (3%) Query: 42 QAEESANALPAITINAQSPLPLQNQVSV-GSNLDLTPL-QTPASVSVINRQQLEERGDAS 99 Q +A ALP I+++A N S G+ TPL + P SV V+ QQ+++ G Sbjct: 41 QEGGTAAALPEISVSAGREKNSYNAPSTSGATRTDTPLLELPQSVRVMTTQQIDDLGAVR 100 Query: 100 ITDAITRAPGYSAMGHPGNSGSALSARGFTDATSVMRLYDGLRQYGGVGVSFPFDTWNID 159 + D + G S + G + + RGF+ +T + L +G G G + DT I+ Sbjct: 101 LADTVDYVSGISRLNDFGGTWDNFAIRGFS-STDMGFLVNGFP--GSRGYNPRRDTATIE 157 Query: 160 RIEVLSGPASVIYGDGAIGGVVNVIPKKPTRGKIENEIETTIGTHNTRRLGLGSGGAINE 219 RIE L GPAS +YG GG +N++ KKP + N E + GT RR + S G +++ Sbjct: 158 RIEFLKGPASALYGSSEPGGTINIVTKKP-QFSASNSAEVSAGTGGLRRATVDSTGPLSQ 216 Query: 220 MLSYRV 225 L+YR+ Sbjct: 217 NLAYRL 222 >gi|300311660|ref|YP_003775752.1| TonB-dependent receptor protein [Herbaspirillum seropedicae SmR1] Length = 705 Score = 80.5 bits (197), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 63/202 (31%), Positives = 97/202 (48%), Gaps = 13/202 (6%) Query: 17 LPAQLRRTIAYAPYALPLLCLAAPVQAEESANALPAITINAQSPLP-------LQNQVSV 69 +PA R T+ + LL LAA A ALP + I A + + + + Sbjct: 1 MPAN-RPTLTFLAVQCALLTLAA--PAWAQQQALPQVEIKADAETANGPVNGYVAKRTAS 57 Query: 70 GSNLDLTPLQTPASVSVINRQQLEERGDASITDAITRAPGYSAMGHPGN--SGSALSARG 127 + D L+ P SVSV+ R +++ RG S+ D + PG ++ H + + RG Sbjct: 58 ATKTDTPVLEVPQSVSVVTRDEMDARGVTSLLDVLRYMPGANSETHGVDPRGYEYFNLRG 117 Query: 128 FTDATSVMRLYDGLRQYGGVGVSFPFDTWNIDRIEVLSGPASVIYGDGAIGGVVNVIPKK 187 F +A +GLRQ G F +T+ ++RIEVL GP SV +G GGV+N + K Sbjct: 118 FINAQVTSNFLNGLRQIPGGFGQFRTETYGMERIEVLRGPGSVTFGQADPGGVINRVSKL 177 Query: 188 PTRGKIENEIETTIGTHNTRRL 209 P I NE+ IG+ N +++ Sbjct: 178 PGM-DIPNELGVDIGSFNRKQV 198 >gi|300312681|ref|YP_003776773.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 738 Score = 79.7 bits (195), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 67/252 (26%), Positives = 124/252 (49%), Gaps = 17/252 (6%) Query: 59 SPLPLQNQ----VSVGSNLDLTPLQ---TPASVSVINRQQLEERGDASITDAITRAPGYS 111 +P+ +Q Q S G+ L P P SV+V+N+ Q++ +G +S++DA+ G + Sbjct: 43 APVTVQGQRDDFASTGTALTKLPADLRDVPQSVTVVNKAQMQSQGASSLSDALRNVSGIT 102 Query: 112 AMGHPGNS-GSALSARGFTDATSVMRLYDGLRQYGGVGVSFPFDTWNIDRIEVLSGPASV 170 G G G+ ++ GF+ T + DG R G + DT+ ID +EVL GP+S+ Sbjct: 103 LGGAEGGQIGNNINLNGFSARTDIY--LDGFRDRG----QYYRDTFAIDEVEVLMGPSSM 156 Query: 171 IYGDGAIGGVVNVIPKKPTRGKIENEIETTIGTHNTRRLGLGSGGAINEMLSYRVD-IAG 229 ++G G+ GG++N + KK K EI +I T+ R + +++ + R++ +A Sbjct: 157 LFGRGSTGGIINQVSKKANL-KAATEISGSITTNGLVRSTVDVNRPLSDTSALRIEAMAQ 215 Query: 230 DRSDGWVDRGNTNNLSLSGALRLDVNRDLFVQLSYAQGHQRPMAYFGTPLINGQQVDALR 289 + + + + + L+G+ + + LS H + +G P +NG + R Sbjct: 216 NGAASTRKQTDVQDFGLAGSYVWGIGTPTEITLSALLQHNQDQPDYGLPPLNGHPANVGR 275 Query: 290 ERNYNV-ADGTI 300 + Y + +D TI Sbjct: 276 DTAYGLNSDRTI 287 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 75.9 bits (185), Expect = 6e-15, Method: Compositional matrix adjust. Identities = 70/260 (26%), Positives = 118/260 (45%), Gaps = 30/260 (11%) Query: 29 PYALPLLCLAAPVQAEE----------SANALPAITINAQ--SPLPLQNQVSVGSNLDLT 76 P L L LA P A E ++LP + + Q SP P +V + Sbjct: 17 PATLCLAFLALPAYAAERDGGNEGEPSGESSLPVVEVRDQRRSPDPRARRVGTATRSAAD 76 Query: 77 PLQTPASVSVINRQQLEERGDASITDAITRAPGYSAMGHPGNSGSALSARGFTDATSVMR 136 P++ P S+ ++ +Q+ G ++ A+ PG S++ A RGF+ A + Sbjct: 77 PMEVPQSIQSVSVEQVSVYGARTLAGALLGVPGISSISD--TRFDAFRIRGFSSANDL-- 132 Query: 137 LYDGLR---QY-GGVGVSFPFDTWNIDRIEVLSGPASVIYGDGAIGGVVNVIPKKPTRGK 192 L DG+R QY G+G NI+R+EVL GPASV+YG G+ GGV+N I K+P + Sbjct: 133 LLDGMRDDPQYVRGLG--------NIERVEVLRGPASVLYGRGSGGGVINRISKQPGQ-D 183 Query: 193 IENEIETTIGTHNTRRLGLGSGGAINEMLSYRVDIAGDRSDGWVDRGNTNNLSLSGALRL 252 + ++ ++G+ L + + R++ + + + D N + AL+ Sbjct: 184 VASQATLSLGSAGLLGAALDWNRPLAPHWALRINTGREHAGSFRDTVNGTRQYFAPALKW 243 Query: 253 DVNRD-LFVQLSYAQGHQRP 271 + RD +Q Y + + P Sbjct: 244 ESGRDSWLLQAEYDEYERVP 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,387,188 Number of extensions: 59510 Number of successful extensions: 208 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 196 Number of HSP's successfully gapped: 17 Length of query: 457 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 369 Effective length of database: 1,203,190 Effective search space: 443977110 Effective search space used: 443977110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 132 (55.5 bits)