BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0011.0021.p (584 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300314042|ref|YP_003778134.1| signal transduction protein con... 308 1e-84 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 300 3e-82 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 294 2e-80 gi|300311453|ref|YP_003775545.1| signal transduction containing ... 294 2e-80 gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodies... 292 6e-80 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 308 bits (788), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 168/430 (39%), Positives = 248/430 (57%), Gaps = 10/430 (2%) Query: 137 LQLLAENDPLTGLANRYCFDNKLATTLERARQEDMRFGILFMDLDNFKLLNDCYGHSAGN 196 L+ A D LTGL NR+ + L + A ++ ++F+DLD FK +ND GH AG+ Sbjct: 515 LEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKFVNDTLGHKAGD 574 Query: 197 MLLKIVAKRLLGALHPGEMAARLGGDEFAIIAQDISGTQDLIDLSAR----IMKAMEDPF 252 LL+ VA RL A+ + ARLGGDEF +I + QD + L R IM A+ P Sbjct: 575 TLLRTVAGRLRAAVREADTVARLGGDEFIMILPE----QDELTLDTRSLQRIMDAVAAPV 630 Query: 253 VFDNIEQIVTCSIGIASYPECARSAADLCRYADLAMYQVKNTHRNGFYFYSEQLQAKVLY 312 +TCS+G+A YP L ++AD+AMY+ K T RN F FY+ + + L Sbjct: 631 TLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQFYTPAMNEQALE 690 Query: 313 RMGIENALRSKDLAEQLRVFYQPFVDANSLAICGAEVLVRWQHPLLGLLAPDRFISIAEE 372 R+ IE LR+ + + YQP VD S + G+E L+RWQHP LG+++P RFI +AEE Sbjct: 691 RLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGMVSPARFIGLAEE 750 Query: 373 CGMMAIIDGHTRQASLRQLRAWREEGMIDKSFQMAINISASTLRDRSFPDAFRRDLERTD 432 G++ I +A+ Q R W+ G D S +++N+S + + LE T Sbjct: 751 TGLILPIGAWVLRAACTQCRQWQLGGYADIS--VSVNLSVGQFLQKDLVSSIADVLEDTG 808 Query: 433 LDDVRLSVEVTENILVADFTNAAAALTQLREMGVIIALDDFGTGYSSMSYLRQLPISVLK 492 L L +E+TE++++ D +A L L+++GV +++DDFGTGYSS++YL++ PI VLK Sbjct: 809 LPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLAYLKRFPIDVLK 868 Query: 493 IDKSFMQQVPQSPEDVRLLRSIITLAKSLDLEVIVEGVETLAQMQLCQALGADILQGYYF 552 ID+SF++ + +D + SII+LA+ L L VI EGVET Q+ + G D +QGY F Sbjct: 869 IDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRHYGCDEIQGYLF 928 Query: 553 SKPLIASEFE 562 S P+ A +FE Sbjct: 929 SPPVPADKFE 938 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 300 bits (767), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 162/436 (37%), Positives = 250/436 (57%), Gaps = 3/436 (0%) Query: 126 LKLQLLDAHRRLQLLAENDPLTGLANRYCFDNKLATTLERARQEDMRFGILFMDLDNFKL 185 L L A+ L + +D LT L NR +++L + +A +E F ++FMDLD FK Sbjct: 251 LARSLAAANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMFMDLDGFKA 310 Query: 186 LNDCYGHSAGNMLLKIVAKRLLGALHPGEMAARLGGDEFAIIAQDISGTQDLIDLSARIM 245 +ND GH G+ LL VA+RL + + ARLGGDEF I+ D++ +D + ++ +++ Sbjct: 311 INDSLGHHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILV-DLNTPEDAMGVAEKLV 369 Query: 246 KAMEDPFVFDNIEQIVTCSIGIASYPECARSAADLCRYADLAMYQVKNTHRNGFYFYSEQ 305 +A+ PF D E V+ S+GIA YPE + DL AD AMY K + RNG++F+ Sbjct: 370 EAVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPS 429 Query: 306 LQAKVLYRMGIENALRSKDLAEQLRVFYQPFVDANSLAICGAEVLVRWQHPLLGLLAPDR 365 + A ++ LR Q R+ YQP + + GAE L+RW+HP+ GL+APD+ Sbjct: 430 MNANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQ 489 Query: 366 FISIAEECGMMAIIDGHTRQASLRQLRAWREEGMIDKSFQMAINISASTLRDRSFPDAFR 425 FI +AE G++ I + RQ+R W G +D + M++N+SA D + R Sbjct: 490 FIGLAERSGLILPIGAWVLDEACRQMREWFALGYVD--WTMSVNLSALQFADPHLLEVVR 547 Query: 426 RDLERTDLDDVRLSVEVTENILVADFTNAAAALTQLREMGVIIALDDFGTGYSSMSYLRQ 485 LER L L++EVTE+ + D + L Q+ ++GV I++DDFGTGYSS+ YL++ Sbjct: 548 TTLERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKR 607 Query: 486 LPISVLKIDKSFMQQVPQSPEDVRLLRSIITLAKSLDLEVIVEGVETLAQMQLCQALGAD 545 LP + LKID+ F++++ Q ED ++ +I+ L ++L+L ++ EGVET+ Q A G D Sbjct: 608 LPANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCD 667 Query: 546 ILQGYYFSKPLIASEF 561 LQGY +P+ F Sbjct: 668 ALQGYLMGRPMTPELF 683 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 294 bits (752), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 153/440 (34%), Positives = 255/440 (57%), Gaps = 3/440 (0%) Query: 133 AHRRLQLLAENDPLTGLANRYCFDNKLATTLERARQEDMRFGILFMDLDNFKLLNDCYGH 192 A R++ LA D LTGL NR+ ++ + A + ++ MDLD FK +ND +GH Sbjct: 662 ADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGH 721 Query: 193 SAGNMLLKIVAKRLLGALHPGEMAARLGGDEFAIIAQDISGTQDLIDLSARIMKAMEDPF 252 G +L+ +A+R +L ++ AR+GGDEF ++ D L +++ +++ PF Sbjct: 722 ETGEAVLQEIAQRFRNSLRELDILARVGGDEFIVLVDDFDDPVQLGEIAQKLLTEARKPF 781 Query: 253 VFDNIEQIVTCSIGIASYPECARSAADLCRYADLAMYQVKNTHRNGFYFYSEQLQAKVLY 312 + D E +++ SIGIA+YP +A L + AD+AMY+ K+ ++ + F+S+++ + Sbjct: 782 IIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEINTNTVE 841 Query: 313 RMGIENALRSKDLAEQLRVFYQPFVDANSLAICGAEVLVRWQHPLLGLLAPDRFISIAEE 372 R+ +E LR + V YQP ++ I GAE LVRWQHPL GLL P FI++AEE Sbjct: 842 RIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFITLAEE 901 Query: 373 CGMMAIIDGHTRQASLRQLRAWREEGMIDKSFQMAINISASTLRDRSFPDAFRRDLERTD 432 ++ I + R + R++ M +++IN++ S D D + ++R Sbjct: 902 TRLIDQIGLLVLDVACRDIGLMRQQQM--TCGRISINLTGSQFGDERLLDDIKEVVDRHG 959 Query: 433 LDDVRLSVEVTENILVADFTNAAAALTQLREMGVIIALDDFGTGYSSMSYLRQLPISVLK 492 L E+TE++++ + A A + +R G I++DDFGTGYSS++YL++ P++ LK Sbjct: 960 TPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLK 1019 Query: 493 IDKSFMQQVPQSPEDVRLLRSIITLAKSLDLEVIVEGVETLAQMQLCQALGADILQGYYF 552 IDKSF+ +P +P ++++II ++ +L+L+V+ EGVET Q+Q + G+D QGYYF Sbjct: 1020 IDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYF 1079 Query: 553 SKPLIASEFEYSLLAADSNR 572 SKP+ ++F LLA +R Sbjct: 1080 SKPVPYAQF-VQLLAGQQDR 1098 >gi|300311453|ref|YP_003775545.1| signal transduction containing PAS/GGDEF/EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 683 Score = 294 bits (752), Expect = 2e-80, Method: Compositional matrix adjust. Identities = 175/438 (39%), Positives = 247/438 (56%), Gaps = 7/438 (1%) Query: 132 DAHRRLQLLAENDPLTGLANRYCFDNKLATTLERARQEDMRFGILFMDLDNFKLLNDCYG 191 +A + + LA D LTGL NR + LE AR E++ ++F+DL+ FK +ND G Sbjct: 235 NAEKLIHHLAYYDALTGLPNRTLLTRTVDQALEEARHENLHGCVMFIDLNRFKPINDTLG 294 Query: 192 HSAGNMLLKIVAKRLLGALHPGEMAARLGGDEFAIIAQDISGTQDLIDLSARIMKAMEDP 251 H AG+MLL VA RL AL ++ ARLG DEFA+ I ++ +++ +DP Sbjct: 295 HVAGDMLLVEVAMRLRRALREQDVVARLGADEFAVALFGIDKDSPAY-VAQKLLALFDDP 353 Query: 252 FVFDNIEQIVTCSIGIASYPECARSAADLCRYADLAMYQVKNTHRN---GFYFYSEQLQA 308 F D E V SIG++ YPE A L R AD+AMY+ K N G+ YSE++ Sbjct: 354 FFIDGHELRVGASIGVSLYPENATDTETLLRLADIAMYRAKQGGENNEGGYACYSEEMNR 413 Query: 309 KVLYRMGIENALRSKDLAEQLRVFYQPFVDANSLAICGAEVLVRWQHPLLGLLAPDRFIS 368 L + IE L +L ++YQP VD +LAI GAE LVRWQHP GLL P FI Sbjct: 414 NTLDLLRIETGLLHAFEYNELLLYYQPKVDMQTLAITGAEALVRWQHPERGLLLPGEFIP 473 Query: 369 IAEECGMMAIIDGHTRQASLRQLRAWREEGMIDKSFQMAINISASTLRDRSFPDAFRRDL 428 IAEE G++ + A RQ RAW++ G+ ++A+N++A RS PD L Sbjct: 474 IAEETGLIVQLSDWVLDAVCRQARAWKDAGL--PPIRIALNVTAREF-TRSLPDRVGAAL 530 Query: 429 ERTDLDDVRLSVEVTENILVADFTNAAAALTQLREMGVIIALDDFGTGYSSMSYLRQLPI 488 ER L L +E+TE++L+ + + ++ +G+ ++LDDFGTGYSS+SYL++ PI Sbjct: 531 ERHMLSGEWLELEITESMLMHSTDRVISIMEKICGLGITVSLDDFGTGYSSLSYLKRFPI 590 Query: 489 SVLKIDKSFMQQVPQSPEDVRLLRSIITLAKSLDLEVIVEGVETLAQMQLCQALGADILQ 548 + LKID+SF +P D + +II +AK L +VI EGVE Q + G + LQ Sbjct: 591 NTLKIDRSFTMGIPDDTNDCAIASAIIGIAKQLRHKVIAEGVEDEKQFNFLREAGCNELQ 650 Query: 549 GYYFSKPLIASEFEYSLL 566 GY FS+P+ A +F LL Sbjct: 651 GYIFSRPVPAPDFHAMLL 668 >gi|300312904|ref|YP_003776996.1| diguanylate cyclase/phosphodiesterase with PAS/PAC sensor domains protein [Herbaspirillum seropedicae SmR1] Length = 701 Score = 292 bits (747), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 158/422 (37%), Positives = 244/422 (57%), Gaps = 4/422 (0%) Query: 141 AENDPLTGLANRYCFDNKLATTLERARQEDMRFGILFMDLDNFKLLNDCYGHSAGNMLLK 200 A +D LTGL NR L L ARQ+ + F+DLD FK +ND GH G+ LLK Sbjct: 266 ATHDALTGLPNRNMLHEHLQRALRVARQKGALTAVAFVDLDQFKFINDSLGHQVGDELLK 325 Query: 201 IVAKRLLGALHPGEMAARLGGDEFAIIAQD-ISGTQDLIDLSARIMKAMEDPFVFDNIEQ 259 VA+RL L +M AR GGDEF ++ Q+ G + ++ RI+ A+ P+ + E Sbjct: 326 TVAQRLQACLREADMVARQGGDEFVLVLQNQTEGEAGIAEVMQRILAAVARPWQAGDREF 385 Query: 260 IVTCSIGIASYPECARSAADLCRYADLAMYQVKNTHRNGFYFYSEQLQAKVLYRMGIENA 319 VT SIG++ YP + L ++AD AMY+ K RN F F++ + ++V R+ + Sbjct: 386 QVTASIGVSRYPADGKDVESLLKHADSAMYRAKEQGRNNFQFFAPWMDSQVSNRLEMLIN 445 Query: 320 LRSKDLAEQLRVFYQPFVDANSLAICGAEVLVRWQHPLLGLLAPDRFISIAEECGMMAII 379 LR ++ +++YQP + + GAE L+RW+ P G++ PDRFI AEE G++ I Sbjct: 446 LRRALDQDEFKLYYQPKLSLQDGRLIGAEALIRWESPQQGMVPPDRFIPFAEEAGLIVPI 505 Query: 380 DGHTRQASLRQLRAWREEGMIDKSFQMAINISASTLRDRSFPDAFRRDLERTDLDDVRLS 439 + + RQ +AW++ G+ +A+N+S L ++S PD L ++ L L Sbjct: 506 GEWVLRTACRQNQAWQQAGL--PPIPVAVNLSPRQL-NQSLPDFVAGVLAQSGLAASWLE 562 Query: 440 VEVTENILVADFTNAAAALTQLREMGVIIALDDFGTGYSSMSYLRQLPISVLKIDKSFMQ 499 +E+TEN+++ D + A L L+ +G+ I++DDFGTGYSS+SYLR+ P+ LKIDKSF++ Sbjct: 563 LEITENVVMKDAEKSVATLHALKRLGLQISVDDFGTGYSSLSYLRRFPVDALKIDKSFVR 622 Query: 500 QVPQSPEDVRLLRSIITLAKSLDLEVIVEGVETLAQMQLCQALGADILQGYYFSKPLIAS 559 + + + ++++II+LA L+L VI EGVE Q + D +QGYYF KP+ + Sbjct: 623 DIVRDADSAAIVKAIISLAHILNLRVIAEGVEDEEQHAFLKENACDEVQGYYFGKPMEVA 682 Query: 560 EF 561 F Sbjct: 683 VF 684 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.388 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,640,742 Number of extensions: 64406 Number of successful extensions: 347 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 298 Number of HSP's successfully gapped: 44 Length of query: 584 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 494 Effective length of database: 1,193,720 Effective search space: 589697680 Effective search space used: 589697680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)